Article
Biochemistry & Molecular Biology
Roberta Palmulli, Enzo Bresteau, Graca Raposo, Guillaume Montagnac, Guillaume van Niel
Summary: Extracellular vesicles (EVs) are actively involved in melanoma progression by modifying the tumor microenvironment and promoting the formation of pre-metastatic niche. The interaction of tumor-derived EVs with the extracellular matrix (ECM) plays a critical role in promoting persistent tumor cell migration. However, the direct interaction between EVs and ECM components is still uncertain. In this study, we utilized electron microscopy and a pull-down assay to demonstrate that sEVs derived from melanoma cell lines can physically interact with collagen I, forming collagen fibrils coated with sEVs, highlighting the heterogeneity of sEVs' interaction with collagen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Akiko Mammoto, Kienna Matus, Tadanori Mammoto
Summary: The aging population is rapidly increasing, with arterial aging leading to various age-related pathologies such as cardiovascular diseases. Age-related changes in the aortic wall affect stable circulation and lead to organ dysfunction. Remodeling of the extracellular matrix is a hallmark of aortic functional stiffening in aging.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Saskia V. Vijver, Akashdip Singh, Eline T. A. M. Mommers-Elshof, Jan Meeldijk, Ronald Copeland, Louis Boon, Sol Langermann, Dallas Flies, Linde Meyaard, M. Ines Pascoal Ramos
Summary: The tumor microenvironment contains collagen fragments that can mediate T cell suppression through LAIR-1, leading to poor outcomes in cancer patients with high expression levels of MMPs and LAIR-1. Therapeutic strategies targeting the interaction of LAIR-1 with collagen fragments may help to reverse T cell dysfunction and improve immunity in cancer patients.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cardiac & Cardiovascular Systems
S. Sajeesh, Shataakshi Dahal, Suraj Bastola, Simran Dayal, Jimmy Yau, Anand Ramamurthi
Summary: The extracellular matrix (ECM) plays a crucial role in maintaining the structure and function of vascular tissues. Changes in the ECM microenvironment can lead to altered tissue function and exacerbation of vascular pathologies. Stem cell (SC)-based therapies hold potential for ECM repair, but face challenges in cell processing, delivery, and maintaining phenotypic integrity.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Engineering, Biomedical
Claire Robertson, Aimy Sebastian, Aubree Hinckley, Naiomy D. Rios-Arce, William F. Hynes, Skye A. Edwards, Wei He, Nicholas R. Hum, Elizabeth K. Wheeler, Gabriela G. Loots, Matthew A. Coleman, Monica L. Moya
Summary: Breast cancers have limited immune activation and expression of immunotherapy markers. The extracellular matrix may contribute to this limitation. Collagen 4 suppresses T cell function, while fibronectin activates T cells.
Article
Engineering, Biomedical
Adam Rauff, Jason C. Manning, James B. Hoying, Steven A. LaBelle, Hannah A. Strobel, Gregory J. Stoddard, Jeffrey A. Weiss
Summary: This study investigated the influence of immediate extracellular cues on the growth trajectories of angiogenic neovessels, and obtained quantitative relationships describing their effects. The results showed that ECM density and nearby cellular bodies were the strongest predictors of neovessel trajectories, while fibril tracks were associated with direction changes in sprout trajectories.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Review
Biotechnology & Applied Microbiology
F. Urciuolo, G. Imparato, P. A. Netti
Summary: The extracellular microenvironment plays a crucial role in regulating cell functions and tissue development. Misregulation of this cell-ECM interaction can lead to dysfunctional tissues and diseases. Tissue engineering approaches should aim to recapitulate the native cell microenvironment to achieve functional tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Subhajit Konar, Scott M. Bolam, Brendan Coleman, Nicola Dalbeth, Sue R. McGlashan, Sophia Leung, Jillian Cornish, Dorit Naot, David S. Musson
Summary: Tendinopathy is characterized by pathological changes in tendon matrix composition, architecture, and stiffness, with inflammation also playing an important role. This study found that substrate stiffness affects tendon-derived cells and macrophages. Tendon-derived cells showed minor responses to substrate stiffness, while macrophages exhibited a more inflammatory phenotype on non-physiological stiffness substrates. These subtle variations in matrix stiffness may contribute to the onset and progression of tendinopathy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wan-Jing Chen, I-Hsuan Lin, Chien-Wei Lee, Yi-Fan Chen
Summary: Aging leads to decline in skeletal muscle function, with reduced regenerative capacity and changes in extracellular matrix structure. This can result in increased collagen deposition and decreased ECM degradation in aged skeletal muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Xiaodan Shi, Song Zhang, Yue Liu, Bryn Brazile, Jim Cooley, J. Ryan Butler, Sara R. McMahan, Karla L. Perez, Jiazhu Xu, Timothy Eastep, Kytai T. Nguyen, Pietro Bajona, Matthias Peltz, Huajian Gao, Yi Hong, Jun Liao
Summary: Cardiac extracellular matrices, particularly elastin, play important roles in cardiac mechanics. This study investigated the distribution and morphology of cardiac elastin in porcine left ventricles. The results showed location-dependent and depth-dependent epicardial elastin network, a thicker endocardial layer with higher elastin-collagen ratio and denser elastin fiber network, and the presence of elastin fibers in myocardial interstitium connecting cardiomyocyte bundles. The collagen-elastin combination provides mechanical strength and facilitates elastic recoil. The association between elastin fibers and Purkinje cells suggests a protective role during deformations. This study provides a structural basis for further biomechanical investigations and biomimicking of cardiac ECM.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Meng Zhang, Fenghua Zhao, Xue Zhang, Linda A. Brouwer, Janette K. Burgess, Martin C. Harmsen
Summary: This study found that MRC-5 fibroblasts promote collagen fiber remodeling and deposition of fibronectin in the hydrogel, leading to modifications in the ECM microenvironment and promoting vascularization.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Ki Wung Chung, Seh Hyon Song, Man-seok Kim
Summary: The study demonstrated that a mixture of amino acids and copper ions significantly increased the expression of genes and proteins related to ECM in human dermal fibroblasts. Copper ions dose-dependently increased gene expression of elastin and collagen I, while the amino acid-Cu2+ mixture enhanced protein expression of these components. Furthermore, the synergistic effects of amino acids and copper ions on ECM protein synthesis in dermal fibroblasts were highlighted in the results.
MOLECULAR BIOLOGY REPORTS
(2021)
Review
Physiology
Corey Wittig, Robert Szulcek
Summary: This review explores how the ratios of ECM proteins change in cardiovascular diseases and the aged individuum, with a focus on collagen-I, collagen-III, and elastin. The study reveals a tendency for increased collagen-I/collagen-III and decreased elastin/collagen ratios in cardiovascular diseases, highlighting the potential implications for pathological states and differences between human heart and aortic ECM protein ratios.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell & Tissue Engineering
Faiza Farhan, Manjari Trivedi, Priscilla Di Wu, Wei Cui
Summary: This study found that HLCs cultured with collagen showed increased albumin and alpha-1 anti-trypsin expression, reduced AFP, and higher urea secretion compared to HLCs cultured with Matrigel. However, collagen-cultured HLCs exhibited lower CYP3A4 activity and glycogen storage than Matrigel-cultured HLCs. The functional differences in HLCs between collagen and Matrigel cultures resemble hepatic zonation in the liver lobules.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Karen G. Soans, Ana Patricia Ramos, Jaydeep Sidhaye, Abhijeet Krishna, Anastasia Solomatina, Karl B. Hoffmann, Raimund Schluessler, Jochen Guck, Ivo F. Sbalzarini, Carl D. Modes, Caren Norden
Summary: Cell migration is crucial for organismal development, but the influence of physical properties on migration phenomena in vivo remains unclear. In this study, the researchers used zebrafish optic cup formation to investigate the role of extracellular matrix (ECM) properties in cell migration. They found that rim cells migrate over an immobile ECM and require cryptic lamellipodia for movement. The topology of the ECM changes along the migration path and is accompanied by changes in cell-matrix interactions. Matrix porosity is linked to efficient migration.
Article
Dermatology
Jos P. H. Smits, Rene A. M. Dirks, Jieqiong Qu, Merel A. W. Oortveld, Arie B. Brinkman, Patrick L. J. M. Zeeuwen, Joost Schalkwijk, Huiqing Zhou, Hendrik Marks, Ellen H. van den Bogaard
Summary: This study aimed to assess the role of DNA methylation changes in regulating keratinocyte fate. The results suggest that in our in vitro experimental setup, changes in DNA methylation are unlikely to play a significant regulatory role in terminal keratinocyte differentiation.
EXPERIMENTAL DERMATOLOGY
(2021)
Correction
Immunology
Mihai G. Netea, Frances Balkwill, Michel Chonchol, Fabio Cominelli, Marc Y. Donath, Evangelos J. Giamarellos-Bourboulis, Douglas Golenbock, Mark S. Gresnigt, Michael T. Heneka, Hal M. Hoffman, Richard Hotchkiss, Leo A. B. Joosten, Daniel L. Kastner, Martin Korte, Eicke Latz, Peter Libby, Thomas Mandrup-Poulsen, Alberto Mantovani, Kingston H. G. Mills, Kristen L. Nowak, Luke A. O'Neill, Peter Pickkers, Tom van der Poll, Paul M. Ridker, Joost Schalkwijk, David A. Schwartz, Britta Siegmund, Clifford J. Steer, Herbert Tilg, Jos W. M. van der Meer, Frank L. van de Veerdonk, Charles A. Dinarello
Article
Physiology
Dennis M. L. W. Kruk, Marissa Wisman, Harold G. de Bruin, Monique E. Lodewijk, Danique J. Hof, Theo Borghuis, Willeke F. Daamen, Toin H. van Kuppevelt, Wim Timens, Janette K. Burgess, Nick H. T. Ten Hacken, Irene H. Heijink
Summary: This study investigated the repair capacity of lung-derived MSCs (LMSCs) from patients with emphysema, compared to controls, and found that COPD-derived LMSCs showed lower expression of FGF10 mRNA, HGF mRNA, and protein, as well as decorin protein. However, COPD-derived LMSCs demonstrated similar engraftment, proliferation, and functioning abilities on native lung tissue scaffolds compared to controls.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Chemistry, Medicinal
Cintia D. S. Horinouchi, Corien Oostendorp, Dennis Schade, Toin H. van Kuppevelt, Willeke F. Daamen
Summary: Small molecules have the potential to modulate cell fates and activate growth factor signaling in skin regeneration. SUN11602 and ONO-1301 show unique activity profiles in keratinocytes and fibroblasts, respectively, by enhancing the expression of specific genes.
ARCHIV DER PHARMAZIE
(2021)
Article
Chemistry, Applied
Patricia Suurs, Henry van den Brand, Willeke F. Daamen, Shai Barbut
Summary: The study investigated the properties of chicken skin collagen preparations from four types of birds and suggested that collagen from slower and fast growing broilers may be suitable sources for the co-extrusion process. Overall, it was feasible to create stronger films with broiler skins compared to skins of broiler breeders and laying hens.
FOOD HYDROCOLLOIDS
(2022)
Article
Pharmacology & Pharmacy
Rik Oude Egberink, Helen M. Zegelaar, Najoua El Boujnouni, Elly M. M. Versteeg, Willeke F. Daamen, Roland Brock
Summary: In this study, we developed porous collagen scaffolds incorporating peptide-mRNA nanoparticles as off-the-shelf functional biomaterials for regenerative medicine applications. The peptide-mRNA complexes showed uniform distribution in the scaffolds, and the matrices maintained cell attachment and viability. Importantly, the mRNA formulation retained activity after lyophilization and storage at 4 degrees C over two weeks. This research provides a viable alternative to lipid-based mRNA formulations, with better control and lower costs.
Article
Pharmacology & Pharmacy
Merel Gansevoort, Jona Merx, Elly M. M. Versteeg, Isidora Vuckovic, Thomas J. Boltje, Toin H. van Kuppevelt, Willeke F. Daamen
Summary: In this study, the feasibility of using click-to-release chemistry to develop pro-regenerative biomaterials was demonstrated. The release of anti-fibrotics from tunable biomaterials was achieved, thus promoting regeneration in wound healing.
Article
Acoustics
J. Janssen, J. van Drongelen, W. F. Daamen, J. P. C. Grutters
Summary: Early-stage health economic modeling was used to estimate the potential value of an advanced sealing plug for fetal endoscopic tracheal occlusion (FETO) in the context of congenital diaphragmatic hernia (CDH). The model showed that the plug strategy has the potential to be cost-effective, as only a minor reduction in the risk of preterm prelabor rupture of membranes (PPROM) and preterm delivery (PTD) is needed. Continued research and development of the plug strategy is deemed to provide value for money.
ULTRASOUND IN OBSTETRICS & GYNECOLOGY
(2023)
Article
Engineering, Biomedical
Rob T. C. Meuwese, Elly M. M. Versteeg, Joris van Drongelen, Danielle de Hoog, Debora Bouwhuis, Frank P. H. A. Vandenbussche, Toin H. van Kuppevelt, Willeke F. Daamen
Summary: A biocompatible collagen plug with shape memory was developed to prevent premature birth after fetal surgery. The plug expands upon application and seals fetal membrane defects, preventing amniotic fluid leakage and potential premature rupture of fetal membranes (iPPROM).
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Applied
Patricia Suurs, Henry van den Brand, Robin ten Have, Willeke F. Daamen, Shai Barbut
Summary: Co-extrusion is an automated sausage production process that uses a continuous collagen dispersion to encase meat and form sausages. This study investigated the properties of collagen preparations from different sources for co-extrusion. The dispersions showed different rheological and mechanical properties, with American Calf, Dutch Heavy Veal, and Danish Ox and/or Heifer being potentially suitable alternatives.
FOOD HYDROCOLLOIDS
(2023)
Review
Materials Science, Biomaterials
Nancy Avila-Martinez, Merel Gansevoort, Juul Verbakel, Haarshaadri Jayaprakash, Ines Maria Araujo, Marta Vitorino, Gustavo Tiscornia, Toin H. van Kuppevelt, Willeke F. Daamen
Summary: Achieving regeneration in humans has been a long-standing goal, and recent research has focused on the African spiny mouse's injury resistance and tissue regeneration capabilities. This review compares the wound healing and regeneration processes of the African spiny mouse and the axolotl, and identifies consistently upregulated matrisome genes that could be potential candidates for biomaterials aiming to promote regeneration.
BIOMATERIALS SCIENCE
(2023)
Meeting Abstract
Cell Biology
Merel Gansevoort, Denis Barritault, Toin van Kuppevelt, Willeke Daamen
WOUND REPAIR AND REGENERATION
(2022)
Meeting Abstract
Cell Biology
Rob Meuwese, Elly Versteeg, Joris van Drongelen, Aarti Ramchandran, Toin van Kuppevelt, Willeke Daamen
WOUND REPAIR AND REGENERATION
(2022)
Meeting Abstract
Cell & Tissue Engineering
Rob T. C. Meuwese, Elly M. M. Versteeg, Joris van Drongelen, Danielle de Hoog, Debora Bouwhuis, Toin H. van Kuppevelt, Willeke F. Daamen
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Dermatology
H. Niehues, G. Rikken, I. M. van Vlijmen-Willems, D. Rodijk-Olthuis, P. V. Erp, P. L. Zeeuwen, J. Schalkwijk, E. H. van den Bogaard
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.
