Article
Biochemistry & Molecular Biology
Kang Du, Liqiao Hu, Pei Wang, Yanhong Xue
Summary: This study presents an improved and simple protocol for isolating SVs from mouse brain, achieving high yield and maintaining the integrity of membrane-associated proteins. The isolated SVs closely resemble their in vivo counterparts, making them suitable for biochemical analysis.
Review
Pharmacology & Pharmacy
Marija Holcar, Masa Kanduser, Metka Lenassi
Summary: Blood is a rich source of disease biomarkers, including extracellular vesicles (EVs). However, contamination issues such as nonphysiological EVs, protein aggregates, lipoproteins, and viruses can affect the interpretation of downstream EV analysis, highlighting the importance of addressing contaminants in EV isolates from blood plasma for biomarker studies.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Monisha Samuel, Rahul Sanwlani, Mohashin Pathan, Sushma Anand, Ella L. Johnston, Ching-Seng Ang, Maria Kaparakis-Liaskos, Suresh Mathivanan
Summary: Milk contains extracellular vesicles (EVs) that have high-quality proteins and can modulate the immune system. This study found that EVs derived from buffalo milk induced higher cell death in colon cancer cells, highlighting their potential as immunoregulatory and anti-cancer agents.
Article
Biochemistry & Molecular Biology
Marina Alorda-Clara, Jose Reyes, Marita Grimanesa Trelles-Guzman, Monica Florido, Pilar Roca, Daniel Gabriel Pons, Jordi Oliver
Summary: Colorectal cancer is the third most common cancer worldwide, and the lack of early and specific biomarkers leads to late-stage detection. Tumors release extracellular vesicles (EVs) that have various functions and can be used as a source of biomarkers. Bowel lavage fluid (BLF) obtained during colonoscopy is an underutilized sample that represents EVs from tumor cells and has the potential to be a useful research tool. In this study, EVs from BLF were isolated and characterized, confirming their suitability for CRC study and potential as biomarkers for diagnosis and monitoring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Veterinary Sciences
Bahram Talebjedi, Nishat Tasnim, Mina Hoorfar, Gabriela F. Mastromonaco, Marcia De Almeida Monteiro Melo Ferraz
Summary: In recent decades, research on extracellular vesicles (EVs) has grown significantly, particularly in their role in cellular communication and potential as biomarkers of health and disease. However, the importance of embryonic EVs is still limited due to small sample volumes, low EV concentrations, and complex isolation procedures. The use of microfluidics technologies shows promise in optimizing the isolation and characterization of embryonic EVs.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Valentina Moccia, Cinzia Centelleghe, Ilaria Giusti, Antonella Peruffo, Vincenza Dolo, Sandro Mazzariol, Valentina Zappulli
Summary: In this study, extracellular vesicles (EVs) were isolated from a bottlenose dolphin and a Cuvier's beaked whale cell line using two different techniques. The EVs were characterized using nanoparticle tracking analysis, Western blotting, and scanning transmission electron microscopy. Further research on cell-derived EVs will enhance our understanding of cetacean pathophysiology and health status assessment.
Review
Chemistry, Multidisciplinary
Nikki Salmond, Karla C. Williams
Summary: EVs are nanometer sized particles released by cells, playing important roles in cell communication and therapeutic delivery. However, lack of standardization in isolating and characterizing EVs calls for more rigorous methods and standardization.
NANOSCALE ADVANCES
(2021)
Article
Medicine, Research & Experimental
Hyun-Kyung Woo, Young Kwan Cho, Chang Yeol Lee, Haeun Lee, Cesar M. Castro, Hakho Lee
Summary: Extracellular vesicles (EVs) have the potential for disease diagnosis, but are often contaminated by low density lipoproteins (LDLs) when isolated from blood. By studying the difference in electric charge between EVs and LDLs, we developed a method using ion-exchange chromatography to separate EVs from LDLs. This finding improved the analytical signals in EV molecular assays.
Article
Oncology
Gleb O. Skryabin, Svetlana V. Vinokurova, Sergey A. Galetsky, Danila S. Elkin, Alexey M. Senkovenko, Darya A. Denisova, Andrey V. Komelkov, Ivan S. Stilidi, Ivan N. Peregorodiev, Olga A. Malikhova, Oiatiddin T. Imaraliev, Adel D. Enikeev, Elena M. Tchevkina
Summary: This study validates the presence of extracellular vesicles (EVs) in gastric juice (GJ) and demonstrates the feasibility of isolating EVs from GJ using ultracentrifugation. The study also reveals the heterogeneity and composition differences of GJ-derived EVs, and proposes the potential of using GJ-derived EVs as miRNA markers for gastric cancer (GC) screening.
Article
Biophysics
Srinivas Bathini, Shanmugasundaram Pakkiriswami, Rodney J. Ouellette, Anirban Ghosh, Muthukumaran Packirisamy
Summary: A magnetic particle based liquid biopsy chip is proposed in this study for the isolation of EVs, with enhanced capture efficiency through the integration of a 3D mixer and a sedimentation unit. The prepared chip can effectively capture and isolate EVs without affecting their morphology.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Microbiology
Silindile Maphosa, Lucy Novungayo Moleleki
Summary: Research found that Pbr1692 releases various morphologies of OMVs, which transport diverse proteins that play critical roles in causing soft rot disease, increasing virulence in potato and Nicotiana benthamiana, and antibacterial activity against Dickeya dadantii.
Article
Parasitology
Sebastian Miles, Javier Magnone, Joaquin Garcia-Luna, Maria Eugenia Ancarola, Marcela Cucher, Sylvia Dematteis, Friedrich Frischknecht, Marek Cyrklaff, Gustavo Mourglia-Ettlin
Summary: This study provides an in-depth characterization of the tegument of Echinococcus ortleppi protoscoleces, revealing a novel structure termed the 'tegumental vesicular body' and vesicle-like structures possibly involved in endocytic/exocytic routes. Parasite antigens were unevenly localized within tegumental structures, with both being detected in vesicles found within the TVB. The presence of host IgG suggests a possible endocytic route in protoscoleces.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2021)
Article
Multidisciplinary Sciences
Steven J. Biller, Maria del Carmen Munoz-Marin, Steeve Lima, Jorge Matinha-Cardoso, Paula Tamagnini, Paulo Oliveira
Summary: Cyanobacteria are a diverse group of bacteria that produce extracellular vesicles, with their specific functional roles still largely unknown. This paper provides a detailed protocol for isolating, concentrating, and purifying cyanobacterial extracellular vesicles, along with successful methodologies and challenges. By discussing methodologies for quantifying and characterizing vesicle samples, the study aims to encourage further research in this area.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Immunology
Mehrdad Shokouhy, Hamzeh Sarvnaz, Yasaman Taslimi, Mahya Sadat Lajevardi, Sima Habibzadeh, Amir Mizbani, Faezeh Shekari, Mandana Behbahani, Ana Claudia Torrecilhas, Sima Rafati
Summary: This study reports for the first time that L. tarentolae can release EVs and provides evidence that tEVs are able to control the infection in human macrophages, making them a great potential platform for drug delivery, at least for parasitic infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Junli Zhang, Yifan Zhu, Mengting Guan, Yingying Liu, Min Lv, Chongwei Zhang, Hongling Zhang, Zhenzhong Zhang
Summary: Exosomes have potential as cancer biomarkers in clinical applications, but enrichment and detection from complex media remain challenging. This study used iodixanol density gradient centrifugation for exosome isolation and purification, and surface plasmon resonance (SPR) biochip for label-free detection of exosomal PD-L1. The method showed better specificity in distinguishing exosomes with different levels of PD-L1 compared to enzyme-linked immunosorbent assays.
Article
Biochemistry & Molecular Biology
Gulcin Pekkurnaz, Jonathan C. Trinidad, Xinnan Wang, Dong Kong, Thomas L. Schwarz
Article
Biochemistry & Molecular Biology
Rita O. Teodoro, Gulcin Pekkurnaz, Abdullah Nasser, Misao E. Higashi-Kovtun, Maria Balakireva, Ian G. McLachlan, Jacques Camonis, Thomas L. Schwarz
Article
Oncology
Glen W. Humphrey, Elena Mekhedov, Paul S. Blank, Antoine de Morree, Gulcin Pekkurnaz, Kanneboyina Nagaraju, Joshua Zimmerberg
EXPERIMENTAL CELL RESEARCH
(2012)
Article
Biochemistry & Molecular Biology
Julia Mazar, Molly Thomas, Ludmila Bezrukov, Alexander Chanturia, Gulcin Pekkurnaz, Shurong Yin, Sergei A. Kuznetsov, Pamela G. Robey, Joshua Zimmerberg
JOURNAL OF BIOLOGICAL CHEMISTRY
(2009)
Review
Biochemistry & Molecular Biology
Seungyoon B. Yu, Gulcin Pekkurnaz
JOURNAL OF MOLECULAR BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Romain Cartoni, Gulcin Pekkurnaz, Chen Wang, Thomas L. Schwarz, Zhigang He
Article
Biology
Anamika Agrawal, Gulcin Pekkurnaz, Elena F. Koslover
Article
Neurosciences
Meghan J. Rossi, Gulcin Pekkurnaz
CURRENT OPINION IN NEUROBIOLOGY
(2019)
Article
Biochemical Research Methods
Linjing Fang, Fred Monroe, Sammy Weiser Novak, Lyndsey Kirk, Cara R. Schiavon, Seungyoon B. Yu, Tong Zhang, Melissa Wu, Kyle Kastner, Alaa Abdel Latif, Zijun Lin, Andrew Shaw, Yoshiyuki Kubota, John Mendenhall, Zhao Zhang, Gulcin Pekkurnaz, Kristen Harris, Jeremy Howard, Uri Manor
Summary: Point-scanning imaging systems are widely used for high-resolution cellular and tissue imaging, but optimizing resolution, speed, sample preservation, and signal-to-noise ratio simultaneously is challenging. The use of deep learning-based supersampling, known as point-scanning super-resolution (PSSR) imaging, can mitigate these limitations. PSSR facilitates high-resolution, fast, and sensitive image acquisition with otherwise unattainable resolution.
Article
Cell Biology
Himanish Basu, Gulcin Pekkurnaz, Jill Falk, Wei Wei, Morven Chin, Judith Steen, Thomas L. Schwarz
Summary: Glucose influx leads to O-GlcNAcylation of TRAK protein, which activates the mechanism of anchoring mitochondria to the F-actin cytoskeleton by FHL2. Disruption of F-actin restores mitochondrial movement arrested by TRAK.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Mathematical & Computational Biology
A. Leung, D. Ohadi, G. Pekkurnaz, P. Rangamani
Summary: This study investigates the role of MERCs in regulating Ca2+ and ATP dynamics using a computational model, revealing the importance of organelle interactions in predicting Ca2+ dynamics in synaptic signaling. The findings demonstrate that the crosstalk between organelles plays a key role in modulating neuronal energy state.
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2021)
Article
Endocrinology & Metabolism
Gulcin Pekkurnaz, Xinnan Wang
Summary: Mitochondria are crucial organelles with distinct morphology and function. They exhibit heterogeneity within cells and divide into functionally distinct subpopulations. This heterogeneity is important for subcellular functions and is especially crucial for polarized cells like neurons. Understanding mitochondrial heterogeneity and homeostasis in neurons provides insights into their significance in other cell types.