Journal
PLOS ONE
Volume 12, Issue 9, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0184672
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Categories
Funding
- International Foundation for Research in Paraplegia
- Wings for Life Spinal Cord Research Foundation
- Dr. Miriam and Sheldon G. Adelson Medical Research Foundation [R01EY021242, R01GM069808]
- NIH [R01GM069808, P30 HD018655, P30EY012196]
- 3R Research Foundation Switzerland [129-11]
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [U54HD090255, P30HD018655] Funding Source: NIH RePORTER
- NATIONAL EYE INSTITUTE [R01EY021242, P30EY012196] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM069808] Funding Source: NIH RePORTER
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Improving axonal transport in the injured and diseased central nervous system has been proposed as a promising strategy to improve neuronal repair. However, the contribution of each cargo to the repair mechanism is unknown. DRG neurons globally increase axonal transport during regeneration. Because the transport of specific cargos after axonal insult has not been examined systematically in a model of enhanced regenerative capacity, it is unknown whether the transport of all cargos would be modulated equally in injured central nervous system neurons. Here, using a microfluidic culture system we compared neurons co-deleted for PTEN and SOCS3, an established model of high axonal regeneration capacity, to control neurons. We measured the axonal transport of three cargos (mitochondria, synaptic vesicles and late endosomes) in regenerating axons and found that the transport of mitochondria, but not the other cargos, was increased in PTEN/SOCS3 co-deleted axons relative to controls. The results reported here suggest a pivotal role for this organelle during axonal regeneration.
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