Exploiting Microfluidics for Extracellular Vesicle Isolation and Characterization: Potential Use for Standardized Embryo Quality Assessment

On-chip fluorescence microscopy with a random microlens diffuser

(2020) Grace Kuo et al.   OPTICS EXPRESS

Comprehensive profiling of Small RNAs in human embryo-conditioned culture media by improved sequencing and quantitative PCR methods

(2020) Stewart J. Russell et al.   Systems Biology in Reproductive Medicine

MicroRNAs in Small Extracellular Vesicles Indicate Successful Embryo Implantation during Early Pregnancy

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Extracellular vesicles of follicular fluid from heat-stressed cows modify the gene expression of in vitro-matured oocytes

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Embryonic extracellular vesicles as informers to the immune cells at the maternal–fetal interface

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Oxygen tension modulates extracellular vesicles and its miRNA contents in bovine embryo culture medium

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(2019) Zheshun Xiong et al.   Scientific Reports

Oviductal extracellular vesicles interact with the spermatozoon’s head and mid-piece and improves its motility and fertilizing ability in the domestic cat

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Proteomic analysis of urinary extracellular vesicles reveal biomarkers for neurologic disease

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Extracellular vesicle-coupled miRNA profiles in follicular fluid of cows with divergent post-calving metabolic status

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Optimized Isolation of Extracellular Vesicles From Various Organic Sources Using Aqueous Two-Phase System

(2019) Oğuz Kaan Kırbaş et al.   Scientific Reports

Acoustic Enrichment of Extracellular Vesicles from Biological Fluids

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Label-free extraction of extracellular vesicles using centrifugal microfluidics

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Genome-wide DNA methylation patterns of bovine blastocysts derived from in vivo embryos subjected to in vitro culture before, during or after embryonic genome activation

(2018) Dessie Salilew-Wondim et al.   BMC GENOMICS

Extracellular vesicles

(2018) Diego Marin et al.   CURRENT OPINION IN OBSTETRICS & GYNECOLOGY

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(2018) Frederik Johannes Verweij et al.   JOURNAL OF CELL BIOLOGY

Evaluation of an ovary-on-a-chip in large mammalian models: Species specificity and influence of follicle isolation status

(2018) Jennifer B. Nagashima et al.   Journal of Tissue Engineering and Regenerative Medicine

Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma

(2018) Ruzena Stranska et al.   Journal of Translational Medicine

Shedding light on the cell biology of extracellular vesicles

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Rapid isolation and enrichment of extracellular vesicle preparations using anion exchange chromatography

(2018) Nikki Heath et al.   Scientific Reports

An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells

(2018) Suchi Gupta et al.   Stem Cell Research & Therapy

Isolation of Extracellular Vesicles: General Methodologies and Latest Trends

(2018) Maria Yu. Konoshenko et al.   Biomed Research International

Microfluidic affinity separation chip for selective capture and release of label-free ovarian cancer exosomes

(2018) Colin L. Hisey et al.   LAB ON A CHIP

Chromatographic Approaches for Purification and Analytical Characterization of Extracellular Vesicles: Recent Advancements

(2018) Sara Tengattini   CHROMATOGRAPHIA

Detection of extracellular vesicles in the mouse vaginal fluid: Their delivery of sperm proteins that stimulate capacitation and modulate fertility

(2018) Zeinab Fereshteh et al.   JOURNAL OF CELLULAR PHYSIOLOGY

An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming

(2018) Marcia A. M. M. Ferraz et al.   Nature Communications

Isolation of High-Purity Extracellular Vesicles by the Combination of Iodixanol Density Gradient Ultracentrifugation and Bind-Elute Chromatography From Blood Plasma

(2018) Zsófia Onódi et al.   Frontiers in Physiology

Improved bovine embryo production in an oviduct-on-a-chip system: prevention of poly-spermic fertilization and parthenogenic activation

(2017) Marcia A. M. M. Ferraz et al.   LAB ON A CHIP

A nano flow cytometer for single lipid vesicle analysis

(2017) Remo Friedrich et al.   LAB ON A CHIP

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Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation

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EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research

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Identification and characteristics of extracellular vesicles from bovine blastocysts produced in vitro

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Isolation of exosomes from whole blood by integrating acoustics and microfluidics

(2017) Mengxi Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Immunoaffinity based methods are superior to kits for purification of prostate derived extracellular vesicles from plasma samples

(2017) Sabine I. Brett et al.   PROSTATE

A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle

(2017) Shuo Xiao et al.   Nature Communications

A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer

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Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies

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A Review on Micromixers

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Extracellular vesicle mediated intercellular communication at the porcine maternal-fetal interface: A new paradigm for conceptus-endometrial cross-talk

(2017) Mallikarjun Bidarimath et al.   Scientific Reports

An integrated double-filtration microfluidic device for isolation, enrichment and quantification of urinary extracellular vesicles for detection of bladder cancer

(2017) Li-Guo Liang et al.   Scientific Reports

Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side

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Reproducible and scalable purification of extracellular vesicles using combined bind-elute and size exclusion chromatography

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Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems

(2017) Soojeong Shin et al.   Scientific Reports

Micro-ribonucleic acids and extracellular vesicles repertoire in the spent culture media is altered in women undergoing In Vitro Fertilization

(2017) Masood Abu-Halima et al.   Scientific Reports

Human vaginal fluid contains exosomes that have an inhibitory effect on an early step of the HIV-1 life cycle

(2016) Johanna A. Smith et al.   AIDS

Sheathless Focusing and Separation of Diverse Nanoparticles in Viscoelastic Solutions with Minimized Shear Thinning

(2016) Chao Liu et al.   ANALYTICAL CHEMISTRY

MicroRNAs in spent blastocyst culture medium are derived from trophectoderm cells and can be explored for human embryo reproductive competence assessment

(2016) Antonio Capalbo et al.   FERTILITY AND STERILITY

Charge-based precipitation of extracellular vesicles

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Ultrasensitive microfluidic analysis of circulating exosomes using a nanostructured graphene oxide/polydopamine coating

(2016) Peng Zhang et al.   LAB ON A CHIP

A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

(2016) Zheng Zhao et al.   LAB ON A CHIP

Microfluidic devices for the study of sperm migration

(2016) S. S. Suarez et al.   MOLECULAR HUMAN REPRODUCTION

Microfluidics for mammalian embryo culture and selection: where do we stand now?

(2016) Séverine Le Gac et al.   MOLECULAR HUMAN REPRODUCTION

Nanoscale lateral displacement arrays for the separation of exosomes and colloids down to 20 nm

(2016) Benjamin H. Wunsch et al.   Nature Nanotechnology

Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device

(2016) Mitsuru Komeya et al.   Scientific Reports

Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation

(2015) Thomas Scherr et al.   BIOMEDICAL MICRODEVICES

Extracellular Vesicles: Composition, Biological Relevance, and Methods of Study

(2015) MikoŁaj P. Zaborowski et al.   BIOSCIENCE

Simple perfusion apparatus for manipulation, tracking, and study of oocytes and embryos

(2015) Stephanie L. Angione et al.   FERTILITY AND STERILITY

Omics as a window to view embryo viability

(2015) Rebecca L. Krisher et al.   FERTILITY AND STERILITY

Automated Vitrification of Embryos: A Robotics Approach

(2015) Jun Liu et al.   IEEE ROBOTICS & AUTOMATION MAGAZINE

Isolation of extracellular vesicles: Determining the correct approach (Review)

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(2015) Natasa Zarovni et al.   METHODS

Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

(2015) Joel Z. Nordin et al.   Nanomedicine-Nanotechnology Biology and Medicine

How should we choose the ‘best’ embryo? A commentary on behalf of the British Fertility Society and the Association of Clinical Embryologists

(2015) Virginia N. Bolton et al.   Human Fertility

Magnetic chemiluminescent immunoassay for human C-reactive protein on the centrifugal microfluidics platform

(2015) G. Czilwik et al.   RSC Advances

Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol

(2015) Mikhail A. Livshits et al.   Scientific Reports

Genome-Wide DNA Methylation Patterns of Bovine Blastocysts Developed In Vivo from Embryos Completed Different Stages of Development In Vitro

(2015) Dessie Salilew-Wondim et al.   PLoS One

Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay

(2014) Arnold Chen et al.   Biomicrofluidics

Human embryos secrete microRNAs into culture media—a potential biomarker for implantation

(2014) Evan M. Rosenbluth et al.   FERTILITY AND STERILITY

Clinical validation of embryo culture and selection by morphokinetic analysis: a randomized, controlled trial of the EmbryoScope

(2014) Irene Rubio et al.   FERTILITY AND STERILITY

Slow and steady cell shrinkage reduces osmotic stress in bovine and murine oocyte and zygote vitrification

(2014) D. Lai et al.   HUMAN REPRODUCTION

Which set of embryo variables is most predictive for live birth? A prospective study in 6252 single embryo transfers to construct an embryo score for the ranking and selection of embryos

(2014) A. Rhenman et al.   HUMAN REPRODUCTION

A novel “salting-out” procedure for the isolation of tumor-derived exosomes

(2014) Zachary Brownlee et al.   JOURNAL OF IMMUNOLOGICAL METHODS

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(2014) Sofia Nahavandi et al.   LAB ON A CHIP

Integrated immunoisolation and protein analysis of circulating exosomes using microfluidic technology

(2014) Mei He et al.   LAB ON A CHIP

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(2014) Shailender Singh Kanwar et al.   LAB ON A CHIP

Extracellular Vesicles in Luminal Fluid of the Ovine Uterus

(2014) Gregory Burns et al.   PLoS One

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(2013) Fatemeh Momen-Heravi et al.   BIOLOGICAL CHEMISTRY

Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery rates: a randomized controlled trial

(2013) Richard T. Scott et al.   FERTILITY AND STERILITY

Time-lapse parameters as predictors of blastocyst development and pregnancy outcome in embryos from good prognosis patients: a prospective cohort study

(2013) K. Kirkegaard et al.   HUMAN REPRODUCTION

Extracellular vesicles: Exosomes, microvesicles, and friends

(2013) Graça Raposo et al.   JOURNAL OF CELL BIOLOGY

Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles

(2013) Zongxing Wang et al.   LAB ON A CHIP

On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids

(2013) Hendrik Deschout et al.   Nanoscale

Expression and Secretion of Plasma Membrane Ca2+-ATPase 4a (PMCA4a) during Murine Estrus: Association with Oviductal Exosomes and Uptake in Sperm

(2013) Amal A. Al-Dossary et al.   PLoS One

Full in vitro fertilization laboratory mechanization: toward robotic assisted reproduction?

(2012) Marcos Meseguer et al.   FERTILITY AND STERILITY

Microfluidic filtration system to isolate extracellular vesicles from blood

(2012) Ryan T. Davies et al.   LAB ON A CHIP

Microfluidic Western blotting

(2012) A. J. Hughes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Impact of Biofluid Viscosity on Size and Sedimentation Efficiency of the Isolated Microvesicles

(2012) Fatemeh Momen-Heravi et al.   Frontiers in Physiology

Wide-field lensless fluorescent microscopy using a tapered fiber-optic faceplate on a chip

(2011) Ahmet F. Coskun et al.   ANALYST

Cell-Secreted Vesicles in Equine Ovarian Follicular Fluid Contain miRNAs and Proteins: A Possible New Form of Cell Communication Within the Ovarian Follicle1

(2011) Juliano C. da Silveira et al.   BIOLOGY OF REPRODUCTION

The use of morphokinetics as a predictor of embryo implantation

(2011) M. Meseguer et al.   HUMAN REPRODUCTION

Biodegradable Porous Silicon Barcode Nanowires with Defined Geometry

(2010) Ciro Chiappini et al.   ADVANCED FUNCTIONAL MATERIALS

Comparison of three methods for isolation of urinary microvesicles to identify biomarkers of nephrotic syndrome

(2010) Ilse M. Rood et al.   KIDNEY INTERNATIONAL

Centrifugal microfluidics for biomedical applications

(2010) Robert Gorkin et al.   LAB ON A CHIP

Microfluidic isolation and transcriptome analysis of serum microvesicles

(2009) Chihchen Chen et al.   LAB ON A CHIP