Article
Biochemistry & Molecular Biology
Kazuaki Okawa, Eri Tabata, Yuta Kida, Kyohei Uno, Hidetoshi Suzuki, Minori Kamaya, Peter O. O. Bauer, Fumitaka Oyama
Summary: This study investigates the loss of enzymatic activity in the Ym1 protein compared to Chia. It identifies three protein segments responsible for the loss: the catalytic motif residues, exons 6 and 7, and exon 10. The study also finds extensive gene duplication events specific to rodent lineages at the Ym1 locus and suggests that amino acid substitutions in regions involved in chitin recognition, binding, and degradation lead to irreversible inactivation of the protein.
Article
Entomology
Seyedeh Reyhaneh Golzan, Reza Talaei-Hassanloui, Mohammad Homayoonzadeh, Seyed Ali Safavi
Summary: Entomopathogenic fungi play an important role in pest management by producing enzymes that disrupt insect cuticles. In this study, the virulence of different isolates of two EPF species was evaluated against Plodia interpunctella larvae. The results showed that protease and lipase activities were positively correlated with the virulence of the fungal isolates.
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
(2023)
Article
Materials Science, Paper & Wood
Marwah M. Bakri, Aisha M. H. Al-Rajhi, Emad Abada, Olfat M. A. Salem, Abdel-Rahman M. Shater, Mohamed S. Mahmoud, Tarek M. Abdel Ghany
Summary: This study investigated the effects of dead fungal biomass (DFB) on the production of chitinase and antibacterial activity by Aspergillus fumigatus. The results showed that DFB stimulated the synthesis of various secondary metabolites and significantly enhanced chitinase activity. DFB also exhibited antimicrobial activity against various bacteria and fungi.
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Chitinases play a crucial role in the survival of microbial pathogens both during host infection and in the environment. They facilitate adhesion to host cells, act as virulence factors, and provide protection from the host immune system. Understanding their mechanisms can help design new therapeutics to control microbial infections.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agricultural Engineering
Hao Tang, Yuan-Qiu Li, Ming-Jun Wang, Chao-Bing Luo
Summary: This study investigates the mechanisms behind efficient lignocellulosic biomass degradation by Cyrtotrachelus buqueti through transcriptome and microbiome analysis. The gut secretory protein and gut microbiota both show efficiency in degrading cellulose, hemicellulose, and lignin. The composition and abundance of bacteria in different gut regions play a crucial role in lignocellulosic biomass degradation.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biology
Tingting Zhang, Yanjun Huo, Qing Dong, Weiwei Liu, Lu Gao, Jiannan Zhou, Daqi Li, Xueyao Zhang, Jianzhen Zhang, Min Zhang
Summary: This paper focuses on the role of chitinase 5-1 (LmCht5-1) and chitinase 5-2 (LmCht5-2) in the degradation of the serosal and pro-nymphal cuticles during locust embryonic development. RNAi experiments showed that both LmCht5-1 and LmCht5-2 contribute to the failure of development in early and late embryogenesis. Our results suggest that both LmCht5-1 and LmCht5-2 play important roles in the degradation of the serosal and pro-nymphal cuticles during the locust embryonic stage.
Article
Biochemistry & Molecular Biology
Kristie D. Goughenour, Janice Whalin, Jason C. Slot, Chad A. Rappleye
Summary: This study investigated fungal chitinases, revealing that the diversity of chitinases is not supported by the previous assumption of being divided into three major clades, including bacterial-origin chitinases. Furthermore, chitin-binding domains are not confined to specific clades, and predicting chitinase functionality requires more sophisticated models.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Tingting Zhang, Pengjuan Ma, Jiannan Zhou, Yingying He, Weimin Liu, Xiaojian Liu, Xueyao Zhang, Rongrong Yu, Min Zhang, Bernard Moussian, Jianzhen Zhang
Summary: The study revealed the importance of chitin deacetylases CDA1 and CDA2 in the function of cuticle barrier in the migratory locust, affecting fungal infection efficiency and permeability to organophosphorus pesticides. This suggests that the barrier function of the locust cuticle is not only dependent on surface cuticular hydrocarbons but also on an intact procuticle.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Plant Sciences
Nadia Ab Razak, Alan C. Gange, Brian C. Sutton, Asyraf Mansor
Summary: Impatiens glandulifera, a highly invasive weed in Europe, can greatly reduce the diversity of native plants, particularly along river banks where it forms monocultures. The role of arbuscular mycorrhizal fungi in this competition with native species is investigated. The mycorrhizal association appears to have a limited effect on balsam growth during competition, but benefits the native species Plantago lanceolata in competing with balsam. This competition also hinders the colonization of beneficial fungi in other native species, making them more susceptible to insects.
Article
Chemistry, Multidisciplinary
Chuchu Li, Hamed Rajabi, Stanislav N. Gorb
Summary: In this study, the microstructure, material composition, and material properties of the corneal cuticle of the desert locust were investigated using various microscopy techniques and nanoindentation. The results suggest that the fully helicoidal architecture and high proportion of resilin in the corneal cuticle are adaptations for light transmission.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Biology
Karen Stamm, Jan-Henning Dirks
Summary: Insect exoskeletons can react to prolonged exposure to hypergravity conditions, with increased elasticity at lower levels of hypergravity but decreased survivability, body mass, and endocuticle thickness at higher levels.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Ecology
Wanting Li, Lulu Xie, Chunzhang Zhao, Xuefeng Hu, Chunying Yin
Summary: This study investigated the effects of nitrogen fertilization under different water availability conditions on soil microbial biomass and composition. The results showed that nitrogen fertilization had a stronger impact on soil microbial biomass and composition compared to water regime. Especially under low soil water availability, nitrogen fertilization increased soil microbial biomass and altered the composition of soil microbial communities.
Article
Biochemistry & Molecular Biology
Chenyin Lv, Tianyan Gu, Rui Ma, Wei Yao, Yuyang Huang, Jingang Gu, Guogang Zhao
Summary: SaChiB, a chitinase of GH family 19, displays optimal activity at 45°C and pH 8.0, with good stability. It exhibits substrate specificity towards chitin and chitooligosaccharides and has potential applications in industrial waste conversion and agricultural biocontrol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Biomedical
Cailin Casey, Claire Yager, Mark Jankauski, Chelsea M. Heveran
Summary: The thorax is a crucial structure for insect flight, and the properties of the thorax cuticle play a significant role in flight efficiency. This study provides a comprehensive assessment of the thorax cuticle in flying insects with different types of flight muscles. The results reveal variations in cuticle properties based on anatomical region and species. The findings highlight the importance of high-resolution assessment of cuticle for understanding the structural and material heterogeneity of the thorax and its impact on flying insects. Additionally, this research sheds light on the potential benefits of material gradation in the design of biomimetic technologies.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Charlie Woodrow, Darron A. Cullen, Fernando Montealegre-Z, Jose Gonzalez-Rodriguez
Summary: In this study, a novel method using non-invasive Raman spectroscopy was used to identify resilin-rich regions in the insect cuticle. The Raman spectrum of resilin-rich regions was compared to nearby low-resilin cuticle, revealing differences in the peaks associated with tyrosine and dityrosine. Principal component analysis showed differences in the light-scattering properties between resilin-rich and resilin-poor regions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Multidisciplinary Sciences
Pacharaporn Angthong, Tanaporn Uengwetwanit, Sopacha Arayamethakorn, Panomkorn Chaitongsakul, Nitsara Karoonuthaisiri, Wanilada Rungrassamee
SCIENTIFIC REPORTS
(2020)
Article
Biotechnology & Applied Microbiology
Nattapol Arunrattanamook, Rungtiva Wansuksri, Tanaporn Uengwetwanit, Verawat Champreda
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2020)
Article
Physiology
Yuwares Malila, Tanaporn Uengwetwanit, Sopacha Arayamethakorn, Yanee Srimarut, Krittaporn V. Thanatsang, Francesca Soglia, Gale M. Strasburg, Wanilada Rungrassamee, Wonnop Visessanguan
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Multidisciplinary Sciences
Tanaporn Uengwetwanit, Umaporn Uawisetwathana, Sopacha Arayamethakorn, Juthatip Khudet, Sage Chaiyapechara, Nitsara Karoonuthaisiri, Wanilada Rungrassamee
Article
Agriculture, Dairy & Animal Science
Krittaporn Thanatsang, Yuwares Malila, Sopacha Arayamethakorn, Yanee Srimarut, Nantawat Tatiyaborworntham, Tanaporn Uengwetwanit, Atikorn Panya, Wanilada Rungrassamee, Wonnop Visessanguan
Article
Biotechnology & Applied Microbiology
Dede Abdulrachman, Lily Eurwilaichitr, Verawat Champreda, Duriya Chantasingh, Kusol Pootanakit
Summary: A new class 2 CRISPR-Cas system, Cpf1, optimized from Francisella tularensis subsp. novicida U112, was developed as a versatile platform for precise genome modification in filamentous fungi like Aspergillus aculeatus. The study demonstrated that FnCpf1 efficiently induced site-specific double-strand breaks and insertions or deletions through the endogenous NHEJ DNA repair pathway, highlighting the potential of CRISPR/Cpf1 technology for strain improvements and functional genomics in fungi.
Article
Biochemistry & Molecular Biology
Tanaporn Uengwetwanit, Wirulda Pootakham, Intawat Nookaew, Chutima Sonthirod, Pacharaporn Angthong, Kanchana Sittikankaew, Wanilada Rungrassamee, Sopacha Arayamethakorn, Thidathip Wongsurawat, Piroon Jenjaroenpun, Duangjai Sangsrakru, Rungnapa Leelatanawit, Jutatip Khudet, Jasper J. Koehorst, Peter J. Schaap, Vitor Martins dos Santos, Frederic Tangy, Nitsara Karoonuthaisiri
Summary: The study presents the first chromosome-level whole-genome assembly of P. monodon, utilizing long-read Pacific Biosciences (PacBio) and long-range Chicago and Hi-C technologies. This high-quality genome assembly serves as an invaluable resource for genetic improvement and breeding of penaeid shrimp in aquaculture.
MOLECULAR ECOLOGY RESOURCES
(2021)
Article
Multidisciplinary Sciences
Pacharaporn Angthong, Tanaporn Uengwetwanit, Sopacha Arayamethakorn, Wanilada Rungrassamee
Summary: The study identified the establishment of the immune system in shrimp during early larval stages through gene analysis. The Toll pathway was found as a baseline immune system established in the nauplius stage, with immune defense mechanisms like pathogen pattern-recognition proteins, antimicrobial peptides, and heat shock proteins established in later larval stages.
SCIENTIFIC REPORTS
(2021)
Article
Physiology
Yuwares Malila, Tanaporn Uengwetwanit, Krittaporn V. Thanatsang, Sopacha Arayamethakorn, Yanee Srimarut, Massimiliano Petracci, Francesca Soglia, Wanilada Rungrassamee, Wonnop Visessanguan
Summary: The study compared the transcriptomes associated with wooden breast in broilers at two different market ages, revealing differential expression of numerous transcripts in chickens of different ages, particularly impacting metabolism and signaling pathways. Several genes with differential expression related to WB pathology were identified in the FA signaling pathway, indicating the potential involvement of disrupted cell-cell communication in the development of WB myopathy.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biology
Zulema Udaondo, Kanchana Sittikankaew, Tanaporn Uengwetwanit, Thidathip Wongsurawat, Chutima Sonthirod, Piroon Jenjaroenpun, Wirulda Pootakham, Nitsara Karoonuthaisiri, Intawat Nookaew
Summary: This study compares the performance of PacBio and ONT platforms for transcriptomic analysis, finding that read-length bias and sequencing throughput differences are influential factors in transcriptome studies.
Article
Microbiology
Narongrit Jaipolsaen, Siwat Sangsritavong, Tanaporn Uengwetwanit, Pacharaporn Angthong, Vethachai Plengvidhya, Wanilada Rungrassamee, Saowaluck Yammuenart
Summary: Forage preservation for livestock feeding is commonly achieved through drying the plant material or ensiling it into silage. This study compares fermentation quality and bacterial dynamics in whole-plant corn and Napier grass with and without starter inoculation, and finds that the abundance and diversity of epiphytic LAB in plant raw materials may determine whether LAB starter cultures are necessary for silage fermentation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tanaporn Uengwetwanit, Nopporn Chutiwitoonchai, Kanin Wichapong, Nitsara Karoonuthaisiri
Summary: This study analyzed the amino acid sequence conservation of RNA-dependent RNA polymerase (RdRp) across coronaviruses and identified compounds with promising antiviral activity. Molecular dynamics simulations and binding free-energy calculations were performed to elucidate critical interactions, providing a foundation for developing lead compounds effective against SARS-CoV-2.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Multidisciplinary Sciences
Pacharaporn Angthong, Tanaporn Uengwetwanit, Wirulda Pootakham, Kanchana Sittikankaew, Chutima Sonthirod, Duangjai Sangsrakru, Thippawan Yoocha, Intawat Nookaew, Thidathip Wongsurawat, Piroon Jenjaroenpun, Wanilada Rungrassamee, Nitsara Karoonuthaisiri
Article
Microbiology
Kentaro Miyazaki, Apirak Wiseschart, Kusol Pootanakit, Kei Kitahara
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
Article
Environmental Sciences
Wirulda Pootakham, Tanaporn Uengwetwanit, Chutima Sonthirod, Kanchana Sittikankaew, Nitsara Karoonuthaisiri
FRONTIERS IN MARINE SCIENCE
(2020)