Article
Chemistry, Physical
Eva D. Schuiten, Christoffel P. S. Badenhorst, Gottfried J. Palm, Leona Berndt, Michael Lammers, Jan Mican, David Bednar, Jiri Damborsky, Uwe T. Bornscheuer
Summary: The study identified an epoxide hydrolase called CorEH that exhibits dehalogenase activity, suggesting a rare example of a protein with dual enzymatic functions. Despite the much lower dehalogenase activity compared to epoxide hydrolase activity, the presence of multiple activities in one naturally occurring protein demonstrates significant diversity.
Article
Chemistry, Medicinal
Steffen Brunst, Julia Schoenfeld, Peter Breunig, Luisa D. Burgers, Murphy DeMeglio, Johanna H. M. Ehrler, Felix F. Lillich, Lilia Weizel, Jasmin K. Hefendehl, Robert Fuerst, Ewgenij Proschak, Kerstin Hiesinger
Summary: In this study, a small fluorescent sEH inhibitor was designed and showed excellent potency in vitro and the ability to visualize sEH in living cells.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Plant Sciences
Jang Hoon Kim, Ji Hyeon Park, Sung Cheol Koo, Yun-Chan Huh, Mok Hur, Woo Tae Park, Youn-Ho Moon, Tae Il Kim, Byoung Ok Cho
Summary: This study evaluated the efficacy of cis-khellactone, a compound isolated from Peucedanum japonicum, as an anti-inflammatory agent. The compound was found to be a competitive inhibitor of sEH and could inhibit pro-inflammatory cytokines. Molecular docking and dynamics simulations revealed the binding pose of cis-khellactone within the active site of sEH, suggesting its dependency on the Trp336-Gln384 loop. These findings highlight the potential of cis-khellactone as a natural therapeutic candidate for inflammation treatment.
Article
Biotechnology & Applied Microbiology
Vijaya P. P. Godase, V. Ravi Kumar, Ameeta Ravi Kumar
Summary: The recombinant Yleh from Yarrowia lipolytica NCIM 3589 exhibited high epoxide hydrolase activity, showing potential as a biocatalyst for the biosynthesis of enantiopure synthons. Optimal reaction conditions were determined for the conversion of racemic 1,2-epoxyoctane to enantiopure (R)-1,2-octanediol. Deep eutectic solvents were used as co-solvents in the Yleh-catalyzed hydrolysis, leading to increased diol production.
Article
Chemistry, Analytical
Ting Wu, Xiaoyun Xi, Ying Chen, Chao Jiang, Qian Zhang, Guoliang Dai, Yongtao Bai, Weidong Zhang, Ting Ni, Jiandong Zou, Wenzheng Ju, Meijuan Xu
Summary: This study developed a proteomics assay for the simultaneous quantification of microsomal and soluble epoxide hydrolase in rats. The protein quantification was realized through targeted proteomics using liquid chromatography with tandem mass spectrometry. The developed assay was successfully used to quantify the protein levels of microsomal and soluble epoxide hydrolase in rat liver, kidney, and heart S9 samples.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Cell Biology
Menglu Fu, Jing Yu, Zhihui Chen, Ying Tang, Ruolan Dong, Yan Yang, Jinlan Luo, Shuiqing Hu, Ling Tu, Xizhen Xu
Summary: Studies have shown that epoxyeicosatrienoic acids (EETs) have a positive effect on regulating glucose homeostasis in diabetic states by reducing glucose reabsorption and suppressing the expression of SGLT2. Additionally, EETs can ameliorate insulin resistance and diabetes by preventing NF-κB-mediated transcription of SGLT2.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hayley L. Whitfield, Sining He, Yinghong Gu, Colleen Sprigg, Hui -Fen Kuo, Tzyy-Jen Chiou, Andrew M. Riley, Barry V. L. Potter, Andrew M. Hemmings, Charles A. Brearley
Summary: This study describes the differences in substrate specificity between two isoforms of ITPK enzymes (ITPK1 and ITPK4) in Arabidopsis, and provides a molecular explanation for the diverse phosphotransferase activity of ITPK4 by analyzing its ATP-coordinated structure. Furthermore, the study reveals a previously unreported N-terminal haloacid dehalogenase-like fold in ITPK4 and its homologues in other plants.
BIOCHEMICAL JOURNAL
(2023)
Article
Mathematical & Computational Biology
Qiong Wu, Minlin Lin, Peng Wu, Chongyan Zhao, Shuang Yang, Haiying Yu, Wenjiao Xian, Jingfang Song
Summary: This study focused on the protective effect of TPPU on oxidative stress-induced PC-12 cell injury, showing that TPPU increased cell viability and promoted BDNF expression.
COMPUTATIONAL AND MATHEMATICAL METHODS IN MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Ya. P. Kuznetsov, D. A. Pitushkin, E. A. Eshtukova-Shcheglova, V. V. Burmistrov, G. M. Butov, I. A. Novakov
Summary: We have developed an improved synthesis method that simplifies the isolation and purification steps of the target compound. The synthesized compounds exhibit excellent antioxidant properties and antiradical activity.
RUSSIAN CHEMICAL BULLETIN
(2022)
Article
Chemistry, Physical
You-Yi Liu, Meng-Di Wu, Xiu-Xiu Zhu, Xia-Ding Zhang, Chen Zhang, Yao-Hui Xu, Min-Chen Wu
Summary: In this study, regioselectivity of GmEH3 for (S)-1a was improved by reshaping its substrate-binding pocket (SBP), leading to the development of E. coli transformants capable of efficiently synthesizing (R)-1b. Mutagenesis was used to enhance the enzyme's selectivity towards the (R)-1b product, laying the foundation for the synthesis of (R)-hexane-1,2-diol with high ee values.
MOLECULAR CATALYSIS
(2021)
Article
Engineering, Chemical
Zifei Yan, Yubin Wang, Chencan Du, Jian Deng, Guangsheng Luo
Summary: The ring-opening reaction of epoxides with carboxylic acids is commonly conducted in batch stirred reactors, but it suffers from low efficiency and selectivity. In this study, an advanced technology involving a microreactor connected to a stirred reactor was developed to enhance the reaction efficiency and selectivity.
Article
Biochemistry & Molecular Biology
Ryosuke Shinouchi, Keita Shibata, Shiori Jono, Keiji Hasumi, Koji Nobe
Summary: This study investigated the mechanism of the antioxidant and anti-inflammatory effects of SMTP-44D in high glucose treated Schwann cells. The results showed that SMTP-44D significantly increased the ratio of EETs to DHETs and alleviated oxidative stress, inflammation, RAGE induction, and apoptosis after high glucose treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
B. P. Gladkikh, D. Danilov, V. S. D'yachenko, V. V. Burmistrov, G. M. Butov, I. A. Novakov
Summary: A method for the synthesis of 3-fluoro-1-isocyanatoadamantane and a new series of inhibitors were developed. The absence of a methylene bridge between adamantane and urea led to higher melting points, while the introduction of a fluorine atom reduced melting points.
RUSSIAN CHEMICAL BULLETIN
(2022)
Article
Engineering, Chemical
Ye Li Mo, Michael Fischlschweiger, Tim Zeiner
Summary: This study focuses on the modeling and analysis of polymeric aqueous two-phase systems (ATPS) with an immobilized phase. By using a model system composed of polyethylene glycol (PEG) and Dextran, the ATPS with the immobilized Dextran was successfully simulated using the Koningsveldt-Kleintjens model combined with a novel incompressible elastic term. The model was validated through sensitivity analysis and experiments, showing good accordance with experimental data.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Article
Multidisciplinary Sciences
Ji Hyeon Park, Jang Hoon Kim, Seon Il Jang, Byoung Ok Cho
Summary: In this study, the anti-inflammatory effects of DK extracted from Peucedanum japonicum Thunberg were investigated. The results showed that DK reduced the production of pro-inflammatory cytokines and downregulated the expression levels of iNOS and COX-2. DK also inhibited NF-kappa B activation and suppressed the phosphorylation of p38 and JNK MAPK phosphorylation. Furthermore, DK decreased the activity and expression of soluble epoxide hydrolase. These findings suggest that DK could be a potential candidate for the treatment of inflammatory diseases.
Review
Biotechnology & Applied Microbiology
Kuo Zhao, Heng Tang, Bo Zhang, Shuping Zou, Zhiqiang Liu, Yuguo Zheng
Summary: Vitamin B5, also known as D-pantothenic acid (D-PA), is a vital micronutrient with significant physiological functions. It is widely used in various fields such as food, medicine, feed, and cosmetics. This review provides an introduction to the role and applications of D-PA, summarizes its biosynthesis pathways and enzymes, analyzes strategies for excessive D-PA production through cell factory development, and discusses the prospects of microbial production of D-PA.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yu-Qi Dong, Ji-Dong Shen, Long Pan, Ji-Hong Huang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: Fructose, a crucial precursor for rare sugar biosynthesis, was produced by thermophilic glucose isomerases (GI) from Caldicellulosiruptor acetigenus (CAGI), Thermoanaerobacter thermocopriae (TTGI), and Thermotoga petrophila (TPGI). The optimal pH for CAGI and TTGI was found to be 8.0 and maintained at 80% activity in a slightly acidic environment. After 24 hours of incubation at 90 degrees Celsius, the relative residual activities of CAGI and TTGI were 40.6% and 52.6%, respectively. Furthermore, CAGI and TTGI exhibited high catalytic performance with conversion rates of 54.2% and 54.1% after an hour at 85 degrees Celsius with 200 g/L glucose. This study provides valuable enzyme candidates for fructose production in the industry with significant cost reduction.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zhen-Yang Shen, Yi-Feng Wang, Ying Wang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study investigates the metabolic response and crucial genes in the biosynthetic pathway of iota-methionine. The relationship between different pathways and their controlling forces on metabolic flux are also examined. The findings provide insights into the biosynthesis of iota-methionine and offer a new research method for synthesizing other metabolic products in multilevel regulated and multibranched biosynthetic pathways.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Yu-Ke Cen, Ming-han Li, Qi Wang, Jia-Meng Zhang, Jia-Chen Yuan, Yuan-Shan Wang, Zhi-Qiang Liu, Yuguo Zheng
Summary: The industrially applied F. fujikuroi strains are mainly obtained through spontaneous mutagenesis, but their yield is still low. We designed an efficient strain improvement strategy by amphotericin B resistance prescreening and evolutionary engineering, and obtained a strain with a 25% increase in yield and high genetic stability for GAs production.
PROCESS BIOCHEMISTRY
(2023)
Article
Biochemical Research Methods
Kun Niu, Qiang Fu, Zi-Long Mei, Li-Rong Ge, An-Qi Guan, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: L-Methionine, the only sulfur-containing amino acid among the essential amino acids, is mainly produced by chemical methods in industry. This study focuses on the fermentation production of L-methionine by genetically engineered strains. By regulating the biosynthesis pathway and modifying central metabolic pathways and L-cysteine catabolism pathway, the researchers achieved a fermentation yield of 17.74 g/L in a 5 L bioreactor without adding exogenous amino acids. These strategies effectively balance cell growth and L-methionine production and provide insights for the industrial production of L-methionine by microbial fermentation.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Chemistry, Applied
Xiao-Ling Tang, Ning Li, Yan-Lai Liu, Jing-Peng Li, Kai-Xuan Zhao, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: A positive mutant of MetZ from CvMetZ was obtained with improved activity for L-methionine biosynthesis, and was applied for production in a fed-batch approach with optimized reaction conditions.
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Article
Biotechnology & Applied Microbiology
Haonan Wang, Xia Ke, Rui Jia, Lianggang Huang, Zhiqiang Liu, Yuguo Zheng
Summary: In this study, a high-yield GA(3) producing strain of Fusarium fujikuroi was constructed using metabolic modification, transcriptome analysis, and promoter engineering. Over-expression of AreA and Lae1 genes resulted in an initial strain with GA(3) production of 2.78 g L-1. By employing a nitrogen-responsive bidirectional promoter, the production of GA(3) was further increased to 3.02 g L-1. The top 20 upregulated genes in GA(3) over-production were characterized, providing potential targets for further strain development. This work offers an efficient microbial development platform for industrial GA(3) production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Kun Niu, Yu-Xin Qi, Hong-Wei Cai, Yi-Xin Ye, Hai-Yan Zhou, Xiao-Tian Liu, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: The effects of different amino acids on Echinocandin B biosynthesis were investigated. It was found that adding L-threonine did not significantly increase the fermentation titer of Echinocandin B. However, using methyl oleate as a carbon source resulted in a two-fold increase in Echinocandin B production. Transcription level analysis indicated that the gene an655543 related to L-threonine biosynthesis had high expression during fermentation, suggesting that exogenous addition of L-threonine had minimal impact. Additionally, the transcription level of gene ecdA appeared to be the main limiting factor for Echinocandin B biosynthesis. This study provides a foundation for future modifications of Echinocandin B-producing strains.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Han-Lin Liu, Jia-Min Wu, Xin-Tong Deng, Lan Yu, Pu-Hong Yi, Zhi-Qiang Liu, Ya-Ping Xue, Li-Qun Jin, Yu-Guo Zheng
Summary: This study developed a biocatalytic cascade for the production of PPO with high efficiency and low cost. The cascade integrated different enzymes and showed superior catalytic efficiency in the synthesis of PPO from d,l-PPT. It achieved high space-time yield and complete conversion of the substrate.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Zhong-Ce Hu, Chui-Mu Zheng, Yun-Chao Tao, Shu-Nan Wang, Yuan-Shan Wang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study aimed to increase the accumulation of S-adenosyl-L-methionine (SAM) in Saccharomyces cerevisiae by promoting ATP availability. The results showed that the lack of gene sod1 benefited ATP generation and positively regulated SAM synthesis. Additionally, improving substrate assimilation further enhanced SAM production. The SAM concentration of strain Delta SOD1 was increased from 7.3 g L-1 to 10.1 g L-1 in a 5-L fermenter.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Fang-Ying Zhu, Qing Yang, Min Cao, Ken Zheng, Xiao-Jian Zhang, Qi Shen, Xue Cai, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: D-(-)-Pantolactone (DPL) is an important intermediate for producing D-(+)-pantothenate (vitamin B5). In this study, a newly discovered L-pantolactone dehydrogenase (RhoLPLDH) was used for the oxidation of L-(+)-pantolactone (LPL) to ketopantoyl lactone (KPL). Strategies involving chaperone assistance and decreasing induction temperature were employed to achieve soluble expression of RhoLPLDH in E. coli. Directed evolution and hydrophilicity-based engineering were used to enhance the activity and thermostability of RhoLPLDH. A two stages in one-pot method was successfully used to deracemize 1 M D,L-PL with a yield of 91.2%. These results demonstrate the potential of the CM5 catalyst for enzyme cascade deracemization in the production of DPL.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Dong-Xu Jia, Hai Yu, Fan Wang, Li-Qun Jin, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: A non-characterized cellobiose 2-epimerase (CE) from Caldicellulosiruptor morganii (CmCE) was discovered in the NCBI database, and a smart mutation library was constructed to obtain a positive mutant D226G with improved thermostability and catalytic efficiency. Molecular modeling revealed that the improvement of D226G was attributed to the rigidification of the flexible loop, the compactness of the catalysis pocket, and the increment of substrate-binding capability. The yield of lactulose synthesis catalyzed by D226G reached 45.5%, higher than that achieved with CmCE. This study provides insight into the design of efficient CEs for biosynthesis of lactulose.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Biochemical Research Methods
Xia Ke, Zi-Hao Pan, Hong-Fei Du, Yi Shen, Ji-Dong Shen, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study improved the biosynthetic and secretory productivity of 7-Dehydrocholesterol (7-DHC) in Saccharomyces cerevisiae by reinforcing and reengineering the ergosterol pathway genes, introducing a biphasic fermentation system, and overexpressing lipid transporters. The selective secretion of different sterol intermediates was characterized, and the cooperation between Pr-1 and St1 in sterol transport was confirmed. The improved cell biosynthetic capacity offers a novel modification approach for 7-DHC de novo biosynthesis.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Agriculture, Multidisciplinary
Hui Yang, Bo Zhang, Zi-Dan Wu, Li-Feng Chen, Jia-Yuan Pan, Xiao-Ling Xiu, Xue Cai, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study proposed a combinatorial metabolic engineering strategy to develop an efficient Escherichia coli cell factory for L-cysteine production. The researchers investigated the crucial regulatory modes and employed multi-copy gene expression to achieve a two-stage fermentation for improved balance between production and growth. They further optimized carbon flux distribution by modifying the C1 unit metabolism and the glycolytic pathway, and demonstrated the superior performance of thiosulfate utilization pathways in enhancing L-cysteine production. The work also highlighted the vital role of synergistic enhancement of sulfur-carbon metabolism in L-cysteine overproduction. In a 5 L bioreactor, the engineered strain accumulated 12.6 g/L of L-cysteine.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Hui Yang, Bo Zhang, Zi-Dan Wu, Li-Feng Chen, Jia-Yuan Pan, Xiao-Ling Xiu, Xue Cai, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study presents a combinatorial metabolic engineering strategy for the development of an efficient Escherichia coli cell factory dedicated to L-cysteine production. By optimizing crucial regulatory modes, improving the balance between production and growth, optimizing carbon flux distribution, and modifying sulfur assimilation pathways, the study successfully achieved high L-cysteine titer.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
