Review
Biochemistry & Molecular Biology
Anthony L. Sylvester, David X. Zhang, Sophia Ran, Natalya S. Zinkevich
Summary: Reactive oxygen species (ROS) play important roles in health and disease, with both beneficial and harmful effects. Different sources of ROS have different roles in various pathologies. NOX1 is associated with hypertension through ROS production, making it a promising therapeutic target. NOX2 and NOX4 produce hydrogen peroxide, influencing cellular processes. Increased ROS production from NOX5 contributes to atherosclerosis.
Article
Biochemistry & Molecular Biology
Marta Massari, Callum R. Nicoll, Sara Marchese, Andrea Mattevi, Maria Laura Mascotti
Summary: This article investigates the phylogenetic analysis of the NADPH oxidase family in eukaryotes and finds that all known members share a common ancestor. Calcium-dependency is identified as the earliest form of NOX regulation, while the protein-protein mode of regulation is a more recent event generated by gene duplication.
Article
Oncology
Andrew J. Robinson, Sara Davies, Richard L. Darley, Alex Tonks
Summary: In AML patients, over 60% exhibit constitutive activation of NOX2 and increased ROS production. This leads to increased glucose uptake and proliferation in AML cells, as well as changes in carbohydrate metabolism. Metabolomic profiling revealed significant ROS-specific metabolic alterations in sphingolipid metabolism, fatty acid oxidation, purine metabolism, amino acid homeostasis, and glycolysis, providing further evidence of ROS rewiring metabolism in AML.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Tiziana Pecchillo Cimmino, Rosario Ammendola, Fabio Cattaneo, Gabriella Esposito
Summary: Reactive oxygen species (ROS) are highly reactive molecules that can induce cytotoxicity or regulate cellular physiology. Among the ROS, the superoxide anion radical (O2 center dot-) plays a key role in redox signaling, generated by the NADPH oxidase (NOX) enzyme family and the mitochondrial electron transport chain. In this review, the authors discuss the emerging roles of NOX enzymes in modulating cancer cell metabolism and highlight their potential as therapeutic targets for rewiring tumor metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Ajmal Ahmad, Mohd Imtiaz Nawaz, Mohammad Mairaj Siddiquei, Ahmed M. Abu El-Asrar
Summary: NADPH oxidase (NOX) is a major producer of reactive oxygen species (ROS) which may play a role in the early pathogenesis of diabetic retinopathy (DR). NOX4 could downregulate the expression of neuro-survival factors BDNF/SIRT1, while treatment with a plant-derived NOX inhibitor apocynin (APO) may alleviate the deleterious effects caused by hyperglycemia and/or diabetic mimic hypoxic condition in the early stages of DR.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Natia Qipshidze Kelm, Jane C. Solinger, Kellianne M. Piell, Marsha P. Cole
Summary: Connexin 43 (Cx43) plays an important role in conducting physiological responses in microvasculature. Previous studies have shown that the NADPH oxidase (NOX) enzymes, especially NOX2, are involved in cardiac redox biology and left ventricular remodeling after myocardial infarction. This study found that treatment with conjugated linoleic acid (cLA) significantly decreased survival in a murine model of myocardial infarction, possibly due to the disruption of Connexin 43 between myocytes in the ventricle. The mechanism underlying this effect involves the stimulation of NOX2 and the subsequent decrease in Cx43 levels, leading to gap junction dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Yoon-Seok Seo, Jung-Min Park, Jae-Hyeong Kim, Moo-Yeol Lee
Summary: Smoking is a significant risk factor for various diseases due to the production of excessive reactive oxygen species (ROS). This review focuses on how cigarette smoke promotes the formation of ROS and provides key mechanisms involved.
Article
Neurosciences
Daniel Sobrido-Camean, Matthew C. W. Oswald, David M. D. Bailey, Amrita Mukherjee, Matthias Landgraf
Summary: Neurons respond to changes in activity levels through structural changes at pre- and postsynaptic terminals, which are regulated by reactive oxygen species (ROS). In a Drosophila larval neuromuscular junction model, NADPH oxidases Nox and Duox are essential for the growth of presynaptic motor terminals, while Nox is excluded from postsynaptic dendrites. Aquaporins Bib and Drip serve as ROS conduits in the presynaptic motoneuron, and different sources of ROS may regulate different aspects of activity-regulated structural changes in neurons.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Cell Biology
Marc Herb, Alexander Gluschko, Michael Schramm
Summary: Reactive oxygen species (ROS) play important roles in cellular processes but can also lead to diseases, their effects depending on cell type and ROS production conditions. Cellular redox balance, like cell pH and calcium levels, is tightly controlled, with evidence showing that cells regulate ROS production by controlling activation and localization factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Alberto Nasce, Karim Gariani, Francois R. Jornayvaz, Ildiko Szanto
Summary: Nonalcoholic fatty liver disease (NAFLD) is closely associated with insulin resistance and type 2 diabetes mellitus (T2DM), mainly due to cellular oxidative stress. NADPH oxidase enzymes (NOX-es) play a crucial role in regulating cellular events related to metabolism, receptor signal transmission, proliferation, and apoptosis. This review evaluates the potential relevance of NOX enzymes as pharmacological targets to alleviate fatty liver disease.
Article
Biochemistry & Molecular Biology
Ziting Guo, Jinjin Zhao, Meiping Wang, Song Song, Zongliang Xia
Summary: Recent studies have shown that sulfur dioxide (SO2) plays a positive role in stress defense responses in plants, especially in seed germination. SO2 treatment can significantly promote seed germination and vigor in maize by increasing reactive oxygen species levels and NADPH oxidase activities, leading to elevated alpha-amylase activity and transcripts. These findings suggest that SO2 may function as a signaling molecule in plant growth and development, specifically in seed germination control.
Article
Pharmacology & Pharmacy
Sergejs Zavadskis, Adelheid Weidinger, Dominik Hanetseder, Asmita Banerjee, Cornelia Schneider, Susanne Wolbank, Darja Marolt Presen, Andrey Kozlov
Summary: The study found that the inhibitor DPI has different effects on ROS metabolism and mitochondrial function in various types of cells, such as stem cells and differentiated cells, depending on the cell's energy metabolism. Undifferentiated cells may be a better target for DPI compared to differentiated parenchymal cells.
Article
Biology
Qin Wu, Ayse Gurpinar, Maxwell Roberts, Patrizia Camelliti, Michael R. Ruggieri Sr, Changhao Wu
Summary: This study provides evidence that the brain centers controlling urination express Nox 2 and produce ROS, suggesting the importance of Nox-associated ROS production in physiology and pathology. These findings lay the groundwork for future investigation into Nox 2 and the associated oxidative damage in brain urination control centers and consequent bladder abnormalities.
Article
Biochemistry & Molecular Biology
Stephenson B. Owusu, Elodie Hudik, Celine Ferard, Sophie Dupre-Crochet, Eric C. D. K. Addison, Kwasi Preko, Tania Bizouarn, Chantal Houee-Levin, Laura Baciou
Summary: Simulating oxidative stress with gamma irradiation leads to shortened half-lives of neutrophils but pre-activates surviving cells to produce superoxide anions. Incomplete assembly of the NADPH oxidase complex after irradiation significantly enhances neutrophil reactivity.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Chemistry, Medicinal
Seunghwan Shim, Da Un Jeong, Hyemi Kim, Chae Yun Kim, Hyejun Park, Yinglan Jin, Kyung Min Kim, Hwa Jeong Lee, Dong Hwan Kim, Yun Soo Bae, Yongseok Choi
Summary: This study designed and synthesized a series of novel Nox inhibitors, which showed regulatory effects on neuronal inflammation in vitro and in vivo experiments, and demonstrated therapeutic efficacy in a PD animal model.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Chemistry, Medicinal
Jae-Jin Lee, Yeon Seung Park, Kong-Joo Lee
ARCHIVES OF PHARMACAL RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Moon-Jung Kim, Jaeho Jeong, Jihye Jeong, Kwang Yeon Hwang, Kong-Joo Lee, Hwa-Young Kim
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2015)
Article
Oncology
Hyun Jung Kim, Venkataraman Magesh, Jae-Jin Lee, Sun Kim, Ulla G. Knaus, Kong-Joo Lee
Article
Multidisciplinary Sciences
Seong-Yeon Bae, Hyun Jung Kim, Kong-Joo Lee, Kyunglim Lee
SCIENTIFIC REPORTS
(2015)
Article
Biochemistry & Molecular Biology
Soonhwa Song, Jae-Jin Lee, Hee-Jung Kim, Jeong Yoon Lee, Jun Chang, Kong-Joo Lee
MOLECULAR AND CELLULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Jae-Jin Lee, Sura Ha, Hee-Jung Kim, Hyun Joo Ha, Hee-Yoon Lee, Kong-Joo Lee
ACS CHEMICAL BIOLOGY
(2014)
Article
Biochemical Research Methods
Mi-Sun Kim, Jaeho Jeong, Jihye Jeong, Dong-Hae Shin, Kong-Joo Lee
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2013)
Article
Engineering, Electrical & Electronic
Kong-Joo Lee, Jee-Eun Kim, Bo-Hyun Yun
Article
Biochemistry & Molecular Biology
Jae-Jin Lee, Joon Kyu Park, Jaeho Jeong, Hyesung Jeon, Jong-Bok Yoon, Eunice EunKyeong Kim, Kong-Joo Lee
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemical Research Methods
Jin Wook Kim, Sunho Lee, Kunsoo Park, Seungjin Na, Eunok Paek, Hyung Seo Park, Heejin Park, Kong-Joo Lee, Jaeho Jeong, Hwa-Young Kim
JOURNAL OF PROTEOME RESEARCH
(2012)
Review
Spectroscopy
Hee-Jung Kim, Sura Ha, Hee Yoon Lee, Kong-Joo Lee
MASS SPECTROMETRY REVIEWS
(2015)
Article
Multidisciplinary Sciences
Mi-Kyung Kim, Jin-Hwan Cho, Jae-Jin Lee, Ye-Hwang Cheong, Moon-Ho Son, Kong-Joo Lee
Article
Multidisciplinary Sciences
Hee-Jung Kim, Hyun Jung Kim, Jae-Eun Jeong, Jeong Yeob Baek, Jaeho Jeong, Sun Kim, Young-Mee Kim, Youhwa Kim, Jin Han Nam, Sue Hee Huh, Jawon Seo, Byung Kwan Jin, Kong-Joo Lee
Article
Multidisciplinary Sciences
Mi-Kyung Kim, Jin-Hwan Cho, Jae-Jin Lee, Moon-Ho Son, Kong-Joo Lee
Article
Multidisciplinary Sciences
Eun Hye Lee, Kitaik Lee, Geun-Hee Kwak, Yeon Seung Park, Kong-Joo Lee, Kwang Yeon Hwang, Hwa-Young Kim
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)