Article
Biochemistry & Molecular Biology
Elodie Blondel-Tepaz, Marie Leverve, Badr Sokrat, Justine S. Paradis, Milena Kosic, Kusumika Saha, Cedric Auffray, Evelyne Lima-Fernandes, Alessia Zamborlini, Anne Poupon, Louis Gaboury, Jane Findlay, George S. Baillie, Herve Enslen, Michel Bouvier, Stephane Angers, Stefano Marullo, Mark G. H. Scott
Summary: The protein beta-arrestin2 plays a critical role in regulating the Mdm2-p53 signaling axis by promoting the nuclear-cytoplasmic shuttling of Mdm2 and enhancing p53 signaling. While beta-arrestin2 can be SUMOylated, it is the non-covalent interaction between SUMO and beta-arrestin2, mediated by a SUMO interaction motif (SIM), that is essential for its cytonuclear trafficking function. Depletion of the RanBP2/RanGAP1-SUMO nucleocytoplasmic transport hub leads to defective beta-arrestin2 nuclear entry, inhibiting its ability to displace Mdm2 from the nucleus and enhancing p53 signaling in cancer cells.
Review
Plant Sciences
Shumei Fang, Xue Hou, Xilong Liang
Summary: SUMOylation is a crucial posttranslational modification in eukaryotes, involved in metabolic regulation under various stresses. SIZ1, a SUMO E3 ligase, plays a vital role in substrate-specific recognition and facilitates the binding of SUMO proteins to target proteins. This review provides insights into the characteristics of SUMOylation and the role of SIZ1 in plant adaptation to abiotic stresses, offering valuable references for crop improvement and future research on plant responses to environmental stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Xiaoxia Shi, Yixin Du, Shujing Li, Huijian Wu
Summary: SUMOylation, a reversible post-translational modification process, plays crucial roles in cell physiology, including cell cycle regulation, DNA damage repair, and protein trafficking and turnover. Dysregulated SUMO E3 ligases may be associated with the occurrence and development of cancers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Yujiao He, Zhiguo Yang, Chen-si Zhao, Zhihui Xiao, Yu Gong, Yun-Yi Li, Yiqi Chen, Yunting Du, Dianying Feng, Amnon Altman, Yingqiu Li
Summary: The study reveals that PKC-theta phosphorylates RanGAP1 to facilitate the assembly and nuclear import of the RanBP2 subcomplex, shedding light on a new link between TCR signaling and NPC assembly. This phosphorylation-dependent sumoylation of RanGAP1 increases its binding affinity for Ubc9, leading to the formation of the RanBP2 subcomplex.
Article
Plant Sciences
Ruiqiang Lai, Jieming Jiang, Jun Wang, Jinju Du, Jianbin Lai, Chengwei Yang
Summary: SUMOylation is a crucial post-translational modification that regulates protein substrates. The maize SIZ1 homologs ZmSIZ1a, ZmSIZ1b, and ZmSIZ1c were functionally characterized in this study, showing potential roles in development regulation and stress responses. Overexpression of ZmSIZ1c in Arabidopsis resulted in early flowering and improved salt tolerance, suggesting distinct functions among the ZmSIZ1 members.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Cell Biology
Roman Gonzalez-Prieto, Karolin Eifler-Olivi, Laura A. Claessens, Edwin Willemstein, Zhenyu Xiao, Cami M. P. Talavera Ormeno, Huib Ovaa, Helle D. Ulrich, Alfred C. O. Vertegaal
Summary: This study identified proteins interacting with different SUMO isoforms, highlighting the key role of XRCC4 in DNA repair processes.
Article
Plant Sciences
Jieming Jiang, Yun Xie, Jinju Du, Chengwei Yang, Jianbin Lai
Summary: SUMOylation plays a crucial role in regulating diverse cellular processes in eukaryotes. OsMMS21, a SUMO E3 ligase in rice, is involved in developmental processes and auxin signaling transduction. The OsMMS21 mutant displays insensitivity to auxin, resulting in a short-root and dwarfism phenotype.
JOURNAL OF PLANT PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Samuel Smalley, Hanjo Hellmann
Summary: This review provides an overview of how ubiquitin and similar proteins modify target proteins in plants to rapidly change their stability and activity, with a focus on their contribution to stress tolerance. The study also explores the potential use of these pathways for generating more robust crop plants to cope with stress situations.
Article
Microbiology
Ishak D. Irwan, Hal P. Bogerd, Bryan R. Cullen
Summary: The SMC5/6 complex plays a direct role in epigenetically silencing integration-competent HIV-1 proviruses before integration.
NATURE MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rahul M. Nair, Ayshwarya Seenivasan, Bing Liu, Dan Chen, Edward D. Lowe, Sonja Lorenz
Summary: This study demonstrates the use of an activity-based probe, ubiquitin-propargylamine, for reconstituting and analyzing the interactions between ubiquitin and certain HECT ligases. The crystal structure reveals a catalytically critical conformation of the C-terminal tail of the ligase for ubiquitin transfer. Additionally, the probe shows selectivity among HECT domains, suggesting potential for developing specific inhibitors and reporters of HECT ligase activities.
ACS CHEMICAL BIOLOGY
(2021)
Article
Plant Sciences
Hyunhee Joo, Chae Woo Lim, Sung Chul Lee
Summary: This study investigates the influence of SUMOylation on the stability and hormone signaling of the CaDRHB1 protein. The results show that CaDSIZ1-mediated SUMOylation enhances the stability of CaDRHB1 and positively regulates abscisic acid signaling and drought response.
Article
Multidisciplinary Sciences
Laura A. Claessens, Matty Verlaan-de Vries, Ilona J. de Graaf, Alfred C. O. Vertegaal
Summary: The SUMO protease SENP6 plays a crucial role in maintaining genomic stability by disassembling SUMO2/3 polymers from DNA damage response proteins, preventing their trapping at sites of DNA damage and nuclear condensates.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Sujuan Gao, Xueqin Zeng, Jianhao Wang, Yingchao Xu, Chunwei Yu, Yishui Huang, Feng Wang, Keqiang Wu, Songguang Yang
Summary: The study reveals that the Arabidopsis SUMO E3 ligase SIZ1 interacts with HDA6 and negatively regulates the function of HDA6 by affecting the acetylation level of histone H3 and the binding of HDA6 to its target genes.
Article
Cell Biology
Ying Liu, Kejia Liu, Rick F. Thorne, Ronghua Shi, Qingyuan Zhang, Mian Wu, Lianxin Liu
Summary: This article investigates the role of SUMOylation and deSUMOylation of Succinate dehydrogenase (SDH) in regulating cellular metabolism. The study reveals that SDHA is SUMOylated by PIAS3 and deSUMOylated by SENP2, which affects the assembly and activity of the SDH complex. Additionally, acetylation of SENP2 by CBP negatively regulates its deSUMOylation activity, thereby regulating the TCA cycle and electron transport chain activity. This mechanism avoids excessive ROS production and promotes cancer cell survival.
Review
Biochemistry & Molecular Biology
Xiao P. Peng, Xiaolan Zhao
Summary: In this article, the authors discuss the composition, architecture, functions, and mechanisms of the SMC complex Smc5/6 in chromosomal replication and repair, as well as its involvement in disease. The importance of Smc5/6 for growth, genotoxin resistance, and host defense in plants and animals is highlighted. Recent advancements, including structural and single-molecule studies, have provided greater insights into the mechanisms underlying Smc5/6 functions. The integration of recent studies on Smc5/6 has identified emerging features of this unique SMC complex and explains its diverse cellular functions and roles in disease pathogenesis, while also pointing out key areas requiring further investigation.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Stefanie Sommer, Tobias Ritterhoff, Frauke Melchior, Henning D. Mootz
Article
Biochemistry & Molecular Biology
Nicolas Stankovic-Valentin, Katarzyna Drzewicka, Cornelia Koenig, Elmar Schiebel, Frauke Melchior
Article
Multidisciplinary Sciences
Tobias Ritterhoff, Hrishikesh Das, Goetz Hofhaus, Rasmus R. Schroeder, Annette Flotho, Frauke Melchior
NATURE COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Linda Lauinger, Jing Li, Anton Shostak, Ibrahim Avi Cemel, Nati Ha, Yaru Zhang, Philipp E. Merkl, Simon Obermeyer, Nicolas Stankovic-Valentin, Tobias Schafmeier, Walter J. Wever, Albert A. Bowers, Kyle P. Carter, Amy E. Palmer, Herbert Tschochner, Frauke Melchior, Raymond J. Deshaies, Michael Brunner, Axel Diernfellner
NATURE CHEMICAL BIOLOGY
(2017)
Article
Biology
Hao Zhou, Katarzyna Bulek, Xiao Li, Tomasz Herjan, Minjia Yu, Wen Qian, Han Wang, Gao Zhou, Xing Chen, Hui Yang, Lingzi Hong, Junjie Zhao, Luke Qin, Koichi Fukuda, Annette Flotho, Ji Gao, Ashok Dongre, Julie A. Carman, Zizhen Kan, Bing Su, Timothy S. Kern, Jonathan D. Smith, Thomas A. Hamilton, Frauke Melchior, Paul L. Fox, Xiaoxia Li
Article
Cell Biology
Sokrates Stein, Maaike H. Oosterveer, Chikage Mataki, Pan Xu, Vera Lemos, Rick Havinga, Claudia Dittner, Dongryeol Ryu, Keir J. Menzies, Xu Wang, Alessia Perino, Sander M. Houten, Frauke Melchior, Kristina Schoonjans
Article
Cell Biology
Saskia Hutten, Georgia Chachami, Ulrike Winter, Frauke Melchior, Angus I. Lamond
JOURNAL OF CELL SCIENCE
(2014)
Review
Biochemistry & Molecular Biology
Nicolas Stankovic-Valentin, Frauke Melchior
MOLECULAR ASPECTS OF MEDICINE
(2018)
Article
Biochemical Research Methods
Georgia Chachami, Nicolas Stankovic-Valentin, Angeliki Karagiota, Angeliki Basagianni, Uwe Plessmann, Henning Urlaub, Frauke Melchior, George Simos
MOLECULAR & CELLULAR PROTEOMICS
(2019)
Article
Multidisciplinary Sciences
Annette Aichem, Carolin Sailer, Stella Ryu, Nicola Catone, Nicolas Stankovic-Valentin, Gunter Schmidtke, Frauke Melchior, Florian Stengel, Marcus Groettrup
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Sina Barysch, Nicolas Stankovic-Valentin, Tim Miedema, Samir Karaca, Judith Doppel, Thiziri Nait Achour, Aarushi Vasudeva, Lucie Wolf, Carsten Sticht, Henning Urlaub, Frauke Melchior
Summary: This study highlights the role of SUMO as a molecular switch in EGFR signaling, demonstrating the importance of transient deSUMOylation of IRF2BP proteins for the appropriate expression of immediate early genes. Comparative SUMO proteome analyses reveal novel regulators in signal transduction and transcription, such as IRF2BP1, IRF2BP2, and IRF2BPL.
Article
Biochemistry & Molecular Biology
Szymon W. Kmiecik, Katarzyna Drzewicka, Frauke Melchior, Matthias P. Mayer
Summary: The heat shock response is a transcriptional program of organisms to counteract protein homeostasis imbalance, regulated by the transcription factor Hsf1. Post-translational modifications such as phosphorylation and SUMOylation are proposed mechanisms to regulate Hsf1 activity. SUMOylation at lysine 298, enhanced by trimerization and phosphorylation, affects Hsf1 efficiency in response to stress.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Juan F. Correa-Vazquez, Francisco Juarez-Vicente, Pablo Garcia-Gutierrez, Sina Barysch, Frauke Melchior, Mario Garcia-Dominguez
Summary: Post-translational modification through Sumo plays a crucial role in regulating neurogenesis. The sumoylation of key proteins can either positively or negatively affect the process of neurogenesis, highlighting the importance of balanced sumoylation for proper development.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Jorg Schweiggert, Gregor Habeck, Sandra Hess, Felix Mikus, Roman Beloshistov, Klaus Meese, Shoji Hata, Klaus-Peter Knobeloch, Frauke Melchior
Summary: The ubiquitin E3 ligase SCFFbxw5 targets MCAK for proteasomal degradation during G(2), which promotes ciliogenesis starting in the preceding cell cycle phase. Loss of Fbxw5 results in increased MCAK levels at basal bodies and impairs ciliogenesis in the following G(1)/G(0).