Article
Cell Biology
Shidong Lv, Zeyu Wu, Mayao Luo, Yifan Zhang, Jianqiang Zhang, Laura E. Pascal, Zhou Wang, Qiang Wei
Summary: This study investigated the pharmacological effects and targets of ivermectin in prostate cancer. Ivermectin was found to inhibit the proliferation of prostate cancer cells and interact with FOXA1 and Ku70/Ku80, leading to changes in gene expression and repair ability, ultimately resulting in cell death.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Qidong Li, Hanhui Yao, Yichun Wang, Yang Wu, Rick F. Thorne, Youming Zhu, Mian Wu, Lianxin Liu
Summary: The circular RNA circPRKAA1 derived from AMP-activated protein kinase is found to play a crucial role in lipid homeostasis and cancer growth. It enhances fatty acid synthesis, promotes lipid storage, and upregulates specific genes to support cancer cell growth.
Article
Oncology
Chao Gao, Guangxu Jin, Elizabeth Forbes, Lingegowda S. Mangala, Yingmei Wang, Cristian Rodriguez-Aguayo, Paola Amero, Emine Bayraktar, Ye Yan, Gabriel Lopez-Berestein, Russell R. Broaddus, Anil K. Sood, Fengxia Xue, Wei Zhang
Summary: Research found that IK somatic mutations are more common in high-grade and high-stage endometrial cancer patients, who have longer survival periods. These mutations are related to the pathophysiology of EC and have a role in promoting cell cycle progression.
Article
Multidisciplinary Sciences
Andrey Anisenko, Olga Shadrina, Irina Garanina, Marina Gottikh
Summary: DNA-PK is a heterotrimeric complex essential for repairing DNA double strand breaks through the non-homologous end joining pathway. In addition to its role in DNA repair, components of DNA-PK are involved in various cellular processes, with the Ku70/Ku80 heterodimer and Ku70 subunit demonstrating the most independent functions.
Article
Chemistry, Multidisciplinary
Jia-Xu Zhou, Cui-Na Li, Ya-Meng Liu, Su-Qin Lin, Ying Wang, Cen Xie, Fa-Jun Nan
Summary: In this study, novel 9,11-seco-cholesterol derivatives were designed and synthesized as FXR antagonists, with compound 9a exhibiting the best FXR antagonistic activity and reducing the expression of FXR target genes.
Review
Cell Biology
Juan Pablo Rigalli, Dirk Theile, Julie Nilles, Johanna Weiss
Summary: PXR interacts with RXRα to regulate drug metabolism, immune function, and cancer pathogenesis; its activity is regulated by coactivator and corepressor proteins; studies indicate cell- and ligand-specific differences in PXR activation.
Article
Biochemistry & Molecular Biology
Eun Young Kim, Jae Man Lee
Summary: In this study, it was found that activation of NRF2 in autophagy-deficient conditions inhibits the transactivation of FXR. Decreased inductions of FXR target genes were observed in liver-specific Atg7 knockout mice and with the treatment of autophagy inhibitors. Furthermore, enforced NRF2 activation using small molecules effectively reduced the pharmacological activation of FXR in cultured cells. These findings reveal a novel function of the basal autophagy-NRF2 axis in regulating FXR transactivation and provide insights for potential therapeutic strategies in metabolic diseases.
Article
Biochemistry & Molecular Biology
Eun Young Kim, Jae Man Lee
Summary: The FXR receptor is essential for liver function, bile acid synthesis, glucose metabolism, and enzyme activity. Research shows that FXR in intestinal epithelial cells is directly linked to the Mg2+ channel Trpm6, potentially connecting bile acid signaling to Mg2+ homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Andrew D. Huber, William C. Wright, Wenwei Lin, Kinjal Majumder, Jonathan A. Low, Jing Wu, Cameron D. Buchman, David J. Pintel, Taosheng Chen
Summary: This study found that mutating a single residue in the PXR receptor can convert the antagonist SPA70 into an agonist. Molecular dynamics simulations revealed that in certain cases, the action of SPA70 is similar to that of agonists, further elucidating the relationship between the structure and activity of the PXR receptor.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Mark D. Long, Justine J. Jacobi, Prashant K. Singh, Gerard Llimos, Sajad A. Wani, Aryn M. Rowsam, Spencer R. Rosario, Marlous Hoogstraat, Simon Linder, Jason Kirk, Hayley C. Affronti, Andries Bergman, Wilbert Zwart, Moray J. Campbell, Dominic J. Smiraglia
Summary: This study reveals the significant roles of NCOR2 in regulating prostate cancer progression, with reduced NCOR2 expression accelerating disease recurrence and inducing DNA hypermethylation. NCOR2 binds to specific transcription factors and regulates transcription in active enhancer regions.
Review
Biochemistry & Molecular Biology
Sayma Zahid, Murielle Seif El Dahan, Florence Iehl, Paloma Fernandez-Varela, Marie-Helene Le Du, Virginie Ropars, Jean Baptiste Charbonnier
Summary: DNA double-strand breaks are accidental lesions caused by various stresses and genetically programmed events. Most DSBs are repaired in mammalian cells through the classical nonhomologous end-joining pathway, with Ku playing a central role. In addition to its DNA repair function, Ku is involved in various other DNA metabolism processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Emanuela Dylgjeri, Karen E. Knudsen
Summary: DNA-PKcs is a crucial protein kinase involved in cancer development and progression, with its deregulation frequently associated with poor outcomes in various types of tumors. Therapeutic targeting of DNA-PKcs is currently being tested in preclinical and clinical settings for cancer therapy.
Article
Biochemistry & Molecular Biology
Janapriya Saha, Jinsung Bae, Shih-Ya Wang, Huiming Lu, Lori J. Chappell, Purva Gopal, Anthony J. Davis
Summary: This study demonstrates that mutating putative Ku70 phosphorylation sites results in defective DNA damage repair, leading to increased genomic instability and accelerated development of hepatocellular carcinoma.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Janapriya Saha, Jinsung Bae, Shih-Ya Wang, Huiming Lu, Lori J. Chappell, Purva Gopal, Anthony J. Davis
Summary: The study found that mutating putative Ku70 phosphorylation sites results in defective DNA damage repair, leading to genomic instability and accelerated development of HCC.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Cell Biology
Bethan L. Clifford, Leslie R. Sedgeman, Kevin J. Williams, Pauline Morand, Angela Cheng, Kelsey E. Jarrett, Alvin P. Chan, Madelaine C. Brearley-Sholto, Annika Wahlstrom, Julianne W. Ashby, William Barshop, James Wohlschlegel, Anna C. Calkin, Yingying Liu, Anders Thorell, Peter J. Meikle, Brian G. Drew, Julia J. Mack, Hanns-Ulrich Marschall, Elizabeth J. Tarling, Peter A. Edwards, Thomas Q. de Aguiar Vallim
Summary: FXR agonists are used to treat NAFLD by controlling hepatic lipid absorption and fatty acid synthesis, specifically reducing levels of MUFA and PUFA in the liver. This reduction is mediated by repression of specific genes and is independent of other regulatory factors like SHP and SREBP1c. Additionally, tissue-specific FXR KO mice studies show that hepatic FXR controls lipogenic genes while intestinal FXR controls lipid absorption.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)