Article
Biochemistry & Molecular Biology
Wanlu Zhang, Azqa Khan, Jlenia Vitale, Annett Neuner, Kerstin Rink, Christian Luechtenborg, Britta Bruegger, Thomas H. Soellner, Elmar Schiebel
Summary: The short amphipathic alpha-helix of Apq12 regulates the function of Brl1 and Brr6, leading to defects in NPC biogenesis and NE integrity when disrupted. Overexpression of Apq12 promotes over-proliferation of the ONM/ER and accumulation of phosphatidic acid at the NE, possibly during NPC biogenesis.
Article
Biology
Luke K. Davis, Ian J. Ford, Bart W. Hoogenboom
Summary: The rapid transport of biological material to and from the cell nucleus is regulated by the nuclear pore complex (NPC), with a core permeability barrier consisting of FG Nups. Nuclear transport receptors (NTRs) facilitate transport by partitioning in the FG Nup assembly and make up a significant portion of proteins in the NPC barrier. Negative cooperativity was observed in the binding behavior of well-characterized NTRs, NTF2 and Imp-beta, to different planar assemblies of FG Nups, suggesting potential demixed phases of NTRs within the FG Nup assembly that could impact inter-NTR competition and separate transport pathways.
Article
Biochemistry & Molecular Biology
Julie Jacquemyn, Joyce Foroozandeh, Katlijn Vints, Jef Swerts, Patrik Verstreken, Natalia Gounko, Sandra F. Gallego, Rose Goodchild
Summary: Torsin affects lipid metabolism by regulating NEP1R1-CTDNEP1 and Lipin, influencing nuclear envelope remodeling and nuclear pore complex biogenesis. This mechanism explains some of the pleiotropic effects of Torsins.
Article
Multidisciplinary Sciences
Tiantian Zheng, Anton Zilman
Summary: The nuclear pore complexes (NPCs) in eukaryotic cells are responsible for macromolecular transport in and out of the nucleus. The NPCs contain intrinsically disordered proteins (IDPs) and efficiently transport cargoes in crowded conditions. A computational model of the NPC shows that self-regulating mechanisms and the spatial architecture of the IDP assembly contribute to efficient transport.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Cell Biology
Elisa Dultz, Matthias Wojtynek, Ohad Medalia, Evgeny Onischenko
Summary: Nuclear pore complexes (NPCs) are transport channels that cross the nuclear envelope and are composed of nucleoporin proteins. While the overall structure and inventory of nucleoporins are conserved, the composition and assembly pathways of NPCs exhibit significant variability. NPCs appear to be unexchangeable in post-mitotic cells. There are unresolved questions regarding the versatility of NPC assembly and composition, as well as how cells monitor their functional state.
Article
Biology
Manickam Gurusaran, Owen Richard Davies
Summary: The study proposes that the connecting structure formed by SUN-KASH is not a 3:3 complex, but a constitutive 6:6 complex composed of two 3:3 complexes, achieved through different mechanisms. The SUN-KASH 6:6 interface acts as nodes for force distribution and integration across the nuclear envelope, enabling the coordinated transduction of large forces within the cell.
Review
Biochemistry & Molecular Biology
Silvia Santana-Sosa, Emiliano Matos-Perdomo, Jessel Ayra-Plasencia, Felix Machin
Summary: The morphology of the nucleus is typically spherical in eukaryotic cells, but it can change during cell migration and division. This is important because abnormal nuclear morphology is associated with cancer and aging, and targeting nuclear shaping pathways and proteins can be used in therapies. Recent evidence also suggests a connection between nuclear shape and lysosomal function in tumor cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Arianna Penzo, Benoit Palancade
Summary: In this review, the mechanisms of nuclear pore complex (NPC) construction and its role in regulating molecular exchange between the nucleus and cytoplasm are summarized. The study of NPCs not only sheds light on the biogenesis of these complexes but also provides insight into the formation of multiprotein complexes in general.
Article
Multidisciplinary Sciences
Alessio Fragasso, Hendrik W. de Vries, John Andersson, Eli O. van der Sluis, Erik van der Giessen, Andreas Dahlin, Patrick R. Onck, Cees Dekker
Summary: This study successfully reconstituted the selective behavior of the NPC by introducing an artificial FG-Nup that mimics natural Nups, demonstrating that specific spacer sequences or spatial segregation of different FG-motif types are not needed to create selective NPCs.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Alessio Fragasso, Hendrik W. de Vries, John Andersson, Eli O. van der Sluis, Erik van der Giessen, Patrick R. Onck, Cees Dekker
Summary: Nuclear pore complexes (NPCs) regulate molecular transport in eukaryotic cells, and a selective barrier is formed by intrinsically disordered Phe-Gly nucleoporins (FG-Nups). By combining experiments and mathematical modeling, researchers found that Kap95 exhibits two populations when interacting with Nsp1: one population is transported across the pore rapidly, while the other population becomes stably assembled within the FG mesh of the pore.
Review
Oncology
Katherine L. B. Borden
Summary: This review highlights the importance of RNA export in cellular trafficking and the aberrant phenomena observed in cancer. Studies on RNA export machinery and relevant NPC components offer potential therapeutic strategies for targeting cancer.
Article
Biochemistry & Molecular Biology
Jiaojiao Wang, Gaofeng Pei, Yupei Wang, Dewei Wu, Xiaokang Liu, Gaoming Li, Jianfang He, Xiaolin Zhang, Xiaoyi Shan, Pilong Li, Daoxin Xie
Summary: This study revealed that the phase separation of the nuclear pore complex (NPC) central barrier plays a crucial role in regulating plant defense against various biotic stresses. The NUP62 gene was found to positively regulate plant defense against the plant pathogen Botrytis cinerea. Further investigation showed that the phase separation of the NPC central barrier regulates the nucleocytoplasmic transport of immune regulators and activates plant defense against a broad range of biotic stresses.
Article
Biochemistry & Molecular Biology
Merce Gomar-Alba, Vasilisa Pozharskaia, Bogdan Cichocki, Celia Schaal, Arun Kumar, Basile Jacquel, Gilles Charvin, J. Carlos Igual, Manuel Mendoza
Summary: Nuclear pore complexes (NPCs) mediate communication between the nucleus and the cytoplasm and regulate gene expression. Lysine acetyltransferase Esa1 acetylates the nuclear pore basket component Nup60, which recruits the mRNA export factor Sac3, to promote mRNA export. This mechanism also regulates cell entry into S phase and prevents premature commitment to a new cell division cycle in G1 daughter cells.
Article
Biochemistry & Molecular Biology
E. F. Elsiena Kuiper, Sarah M. Prophet, Christian Schlieker
Summary: This study summarizes the assembly process of the nuclear pore complex (NPC) and the defects related to human diseases, emphasizing the need for controlled condensation of phenylalanine-glycine repeat nucleoporins during NPC assembly to prevent aberrant condensation, aggregation, or amyloid formation. Defects in this process are associated with neurological disorders.
Article
Biology
Jacqueline T. Brown, Alexandra J. Haraczy, Christopher M. Wilhelm, Kenneth D. Belanger
Summary: The N-terminal 200 amino acids of Pom152 are sufficient for localization to the NPC, while alterations in other domains may impact Pom152 targeting or affinity for the NPC.
Article
Biochemistry & Molecular Biology
Margaret R. Heider, Mingyu Gu, Caroline M. Duffy, Anne M. Mirzal, Laura L. Marcotte, Alexandra C. Walls, Nicholas Farra, Zhanna Hakhverdyan, Mark C. Field, Michael P. Rout, Adam Frost, Mary Munson
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2016)
Article
Cell Biology
Paul T. Manna, Samson O. Obado, Cordula Boehm, Catarina Gadelha, Andrej Sali, Brian T. Chait, Michael P. Rout, Mark C. Field
JOURNAL OF CELL SCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Paula Upla, Seung Joong Kim, Parthasarathy Sampathkumar, Kaushik Dutta, Sean M. Cahill, Ilan E. Chemmama, Rosemary Williams, Jeffrey B. Bonanno, William J. Rice, David L. Stokes, David Cowburn, Steven C. Almo, Andrej Sali, Michael P. Rout, Javier Fernandez-Martinez
Article
Microbiology
Michael P. Rout, Samson O. Obado, Sergio Schenkman, Mark C. Field
Article
Microbiology
Cordula M. Boehm, Samson Obado, Catarina Gadelha, Alexandra Kaupisch, Paul T. Manna, Gwyn W. Gould, Mary Munson, Brian T. Chait, Michael P. Rout, Mark C. Field
Review
Biochemistry & Molecular Biology
Yinxing Ma, Yegor Vassetzky, Svetlana Dokudovskaya
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2018)
Article
Multidisciplinary Sciences
Yinxing Ma, Licia Silveri, John LaCava, Svetlana Dokudovskaya
SCIENTIFIC REPORTS
(2017)
Review
Cell Biology
Olga V. Iarovaia, Elizaveta P. Minina, Eugene V. Sheval, Daria Onichtchouk, Svetlana Dokudovskaya, Sergey V. Razin, Yegor S. Vassetzky
TRENDS IN CELL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Burkitkan Akbay, Diego Germini, Amangeldy K. Bissenbaev, Yana R. Musinova, Evgeny V. Sheval, Yegor Vassetzky, Svetlana Dokudovskaya
Summary: HIV-1 Tat activates the Akt/mTORC1 signaling pathway in B cells, leading to aberrant activation of AICDA and inhibition of AICDA transcriptional repressors c-Myb and E2F8. These disruptions may ultimately lead to increased genomic instability and proliferation, potentially causing B cell malignancies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Dawei Liu, Felix Peyre, Yahir Alberto Loissell-Baltazar, Delphine Courilleau, Sandra Lacas-Gervais, Valerie Nicolas, Eric Jacquet, Svetlana Dokudovskaya, Frederic Taran, Jean-Christophe Cintrat, Catherine Brenner
Summary: This study identified six compounds through high-throughput screening that have the potential to prevent cardiac cell death caused by chemotherapy, suggesting their promising role as cardioprotective drugs.
Review
Cell Biology
Yahir A. Loissell-Baltazar, Svetlana Dokudovskaya
Summary: The SEA complex was first described in yeast and its human homologue, the GATOR complex, two years later. Over the past decade, significant advances have been made in understanding the multiple functions of the SEA/GATOR complex in different organisms, highlighting its role as an essential upstream regulator of the mTORC1 pathway. Additionally, the consequences of GATOR mutations in cancer and neurodegenerative diseases have been emphasized.
Review
Biochemistry & Molecular Biology
Zhenrui Pan, Hanxiao Zhang, Svetlana Dokudovskaya
Summary: Cisplatin is a platinum-based drug widely used for the treatment of many solid tumors. However, resistance to cisplatin poses a major challenge in anticancer therapy. This review focuses on recent studies exploring the influence of the mTORC1 pathway and autophagy on cisplatin sensitivity and resistance, and discusses their potential for developing new therapeutic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yinxing Ma, Alexis Moors, Nadine Camougrand, Svetlana Dokudovskaya
CELLULAR AND MOLECULAR LIFE SCIENCES
(2019)
Review
Biochemistry & Molecular Biology
Yara Bou Saada, Vlada Zakharova, Boris Chernyak, Carla Dib, Gilles Carnac, Svetlana Dokudovskaya, Yegor S. Vassetzky
CELLULAR AND MOLECULAR LIFE SCIENCES
(2017)
Article
Multidisciplinary Sciences
John LaCava, Hua Jiang, Michael P. Rout
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2016)
