Article
Biotechnology & Applied Microbiology
Karen Julie la Cour Karottki, Hooman Hefzi, Songyuan Li, Lasse Ebdrup Pedersen, Philipp N. Spahn, Chintan Joshi, David Ruckerbauer, Juan A. Hernandez Bort, Alex Thomas, Jae Seong Lee, Nicole Borth, Gyun Min Lee, Helene Faustrup Kildegaard, Nathan E. Lewis
Summary: Media and feed optimization have improved mammalian biopharmaceutical production, but genome editing offers a new avenue for enhancing cell metabolism. A comprehensive pooled CRISPR screen identified a glutamine response network in CHO cells, and knockout of a poorly characterized lipase gene significantly increased cell growth in glutamine-free media. The screen provides a targeted platform to study genes involved in metabolic traits and elucidate novel regulators of metabolism.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Shingo Noguchi, Kenichi Wakita, Fumio Matsuda, Hiroshi Shimizu
Summary: This study compared the metabolic phenotypes of cancer cells cultured in 2D and 3D environments using C-13 metabolic flux analysis. The results demonstrated distinct metabolic characteristics of cells cultured in 3D, showing significant differences in metabolic pathways compared to 2D cultures.
METABOLIC ENGINEERING
(2022)
Article
Immunology
Tiphaine Sancerni, Ophelie Renoult, Angele Luby, Cedric Caradeuc, Veronique Lenoir, Mikael Croyal, Celine Ransy, Esther Aguilar, Catherine Postic, Gildas Bertho, Renaud Dentin, Carina Prip-Buus, Claire Pecqueur, Marie-Clotilde Alves-Guerra
Summary: In this study, researchers identified UCP2 as a metabolite transporter controlling T-ALL cell proliferation. UCP2 plays a crucial role in maintaining cellular metabolism for cell growth and mitochondrial respiration. Interfering with UCP2 function may be a strategy to decrease metabolic efficiency and proliferation rate of leukemia cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Environmental Sciences
Ruijia Zhang, Wenhua Lu, Lanyin Tu, Yingshi Lin, Jin Sun, Baowei Chen, Tiangang Luan
Summary: This study used metabolic flux analysis to investigate the interconnections of glucose, glutamine, and fatty acids in PFOA-treated human colorectal cancer cells. The results showed that PFOA treatment led to metabolic remodeling in intestinal cells, potentially related to the loss of glucose in biomass synthesis and energy metabolism.
ENVIRONMENTAL POLLUTION
(2023)
Article
Oncology
Xianlei Cai, Chao Liang, Miaozun Zhang, Yuan Xu, Yihui Weng, Xueying Li, Weiming Yu
Summary: N6-methyladenosine (m6A) modification plays a significant role in the metabolic reprogramming of digestive system malignancies.
Article
Oncology
Anna Reustle, Lena-Sophie Menig, Patrick Leuthold, Ute Hofmann, Viktoria Stuhler, Christian Schmees, Michael Becker, Mathias Haag, Verena Klumpp, Stefan Winter, Florian A. Buttner, Steffen Rausch, Jorg Hennenlotter, Falko Fend, Marcus Scharpf, Arnulf Stenzl, Jens Bedke, Matthias Schwab, Elke Schaeffeler
Summary: The study found that NNMT protein was overexpressed in ccRCC and correlated with worse survival. NNMT depletion led to intracellular glutamine accumulation, negatively affecting mitochondrial function and cell survival, while NNMT inhibitors provide a novel therapeutic strategy for ccRCC.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Plant Sciences
Yue Yang, Pingya He, Yuhao Hou, Zhicheng Liu, Xiaoping Zhang, Ning Li
Summary: The study found that Osmundacetone (OSC) selectively inhibits the proliferation of non-small cell lung cancer (NSCLC) cells, and suppresses tumorigenesis and cell proliferation by modulating glutamine metabolism and mitochondrial energy metabolism.
Article
Biotechnology & Applied Microbiology
Benjamin Strain, James Morrissey, Athanasios Antonakoudis, Cleo Kontoravdi
Summary: This study evaluates the reliability of Chinese hamster ovary (CHO) cell metabolic models and introduces a new model that outperforms the existing ones in predicting extracellular phenotypes and intracellular fluxes. However, there is still room for improvement in predicting intracellular metabolic states. This work provides an updated CHO cell model and lays the foundation for further research in flux analysis techniques.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Jones Gyamfi, Jinyoung Kim, Junjeong Choi
Summary: Cancer is not only a genetic disease but also a result of metabolic dysregulation. Metabolic alterations in cancer cells, including key changes in glucose, glutamine, and fatty acid metabolism, are more common among different subtypes and types of cancer. Recognizing cancer as a metabolic disorder can reveal important diagnostic and treatment markers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Appalaraju Jaggupilli, Stanley Ly, Khoa Nguyen, Vivek Anand, Bin Yuan, Fouad El-Dana, Yuanqing Yan, Zoe Arvanitis, Danthasinghe Waduge Badrajee Piyarathna, Nagireddy Putluri, Helen Piwnica-Worms, Henry Charles Manning, Michael Andreeff, V. Lokesh Battula
Summary: This study demonstrates that metabolic stress induces the GD2(+) BCSC phenotype in triple-negative breast cancer (TNBC) and highlights the role of glutamine in this phenotype. Inhibiting glutamine transporters can reduce BCSC characteristics in TNBC cells, suggesting a potential complementary approach to conventional chemotherapy.
BRITISH JOURNAL OF CANCER
(2022)
Article
Fisheries
Xue Li, Shidong Wang, Muzi Zhang, Yangping Yu, Ming Li
Summary: In this study, the role of GS in ammonia detoxification was investigated in yellow catfish. The results showed that GS plays a crucial role in regulating glutamine synthesis and ammonia detoxification in yellow catfish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Kai Liao, Qian Zhang, Yangyang Qiu, Yuxiang Zhu, Yang Liu, Hailong Huang, Deshui Chen, Bin Ma, Lin Zhang, Zhaoshou Ran, Xiaojun Yan, Jilin Xu
Summary: It is important to enhance the growth of bivalve aquaculture through nutritional strategies. This study investigated the effects of phosphatidic acid (PA) on the mTOR signaling pathway and metabolic pathways in bivalve mollusk Sinonovacula constricta. The results showed that PA increased glycogen concentration and decreased triglyceride concentration in the muscles. PA also affected the concentrations of various amino acids. Furthermore, PA activated the mTOR signaling pathway and influenced the mRNA levels of key enzymes involved in glycolysis and fatty acid synthesis.
AQUACULTURE RESEARCH
(2023)
Article
Gastroenterology & Hepatology
Tuo Hu, Surendra K. Shukla, Enza Vernucci, Chunbo He, Dezhen Wang, Ryan J. King, Kanupriya Jha, Kasturi Siddhanta, Nicholas J. Mullen, Kuldeep S. Attri, Divya Murthy, Nina Chaika, Ravi Thakur, Scott E. Mulder, Camila G. Pacheco, Xiao Fu, Robin R. High, Fang Yu, Audrey Lazenby, Clemens Steegborn, Ping Lan, Kamiya Mehla, Dante Rotili, Sarika Chaudhary, Sergio Valente, Marco Tafani, Antonello Mai, Johan Auwerx, Eric Verdin, David Tuveson, Pankaj K. Singh
Summary: SIRT5 functions as a key tumor suppressor in PDAC, inhibiting tumor cell proliferation and correlating with a favorable prognosis. Loss of SIRT5 promotes PDAC development and malignant transformation.
Review
Oncology
Nikita Choudhary, Robert C. Osorio, Jun Y. Oh, Manish K. Aghi
Summary: Glioblastoma (GBM) is an aggressive brain tumor with limited prognosis despite multimodal treatment approaches. Various immunotherapies have been investigated to address the need for novel therapeutic options in GBM with limited success. Recently, alterations in the metabolism of cancer cells which allow for tumor proliferation, but simultaneously alter immune populations leading to an immunosuppressive tumor microenvironment, have been investigated as contributory to therapeutic resistance.
Review
Cardiac & Cardiovascular Systems
Xuhan Liu, Liping Zhang, Weihua Zhang
Summary: This article discusses the abnormal metabolism of pulmonary arterial hypertension (PAH) and how metabolic therapy can be used to combat remodeling.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)