Article
Multidisciplinary Sciences
Jasmine Ga May Eng, Mohammadamin Shahsavarani, Daniel Patrick Smith, Josef Hajicek, Vincenzo De Luca, Yang Qu
Summary: This study discovered and characterized vindolinine synthase, an enzyme that catalyzes an unusual redox-neutral reaction, leading to alkaloid diversity. This has important implications for understanding plant specialized metabolism and alkaloid synthesis.
NATURE COMMUNICATIONS
(2022)
Review
Biotechnology & Applied Microbiology
Vyoma Mistry, Siddhi Darji, Pragya Tiwari, Abhishek Sharma
Summary: Catharanthus roseus is a medicinal plant known for biosynthesizing anticancer monoterpenoid indole alkaloids, with more than 130 bioactive compounds synthesized. Due to their cytotoxic nature, the production of these compounds is low in plants. Research has attempted to increase production through plant tissue culture and metabolic engineering.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fanfan Li, Stephen Bordeleau, Kyung Hee Kim, Jonathan Turcotte, Benjamin Davis, Lan Liu, Stephane Bayen, Vincenzo De Luca, Mehran Dastmalchi
Summary: This study described a Catharanthus roseus mutant that accumulates high levels of monoterpenoid indole alkaloids (MIAs). The mutant exhibited stunted growth and deficiency in chlorophyll biosynthesis, along with lesion formation. The lesions were enriched in MIAs and resulted from upregulation of genes involved in the MIA pathway. Furthermore, the accumulation of akuammicine, a minor constituent of the mutant, was found to be inducible by jasmonate and may have a role in plant defense.
Article
Plant Sciences
Ines Carqueijeiro, Konstantinos Koudounas, Thomas Duge de Bernonville, Liuda Johana Sepulveda, Angela Mosquera, Dikki Pedenla Bomzan, Audrey Oudin, Arnaud Lanoue, Sebastien Besseau, Pamela Lemos Cruz, Natalja Kulagina, Emily A. Stander, Sebastien Eymieux, Julien Burlaud-Gaillard, Emmanuelle Blanchard, Marc Clastre, Lucia Atehortua, Benoit St-Pierre, Nathalie Giglioli-Guivarc'h, Nicolas Papon, Dinesh A. Nagegowda, Sarah E. O'Connor, Vincent Courdavault
Summary: Deglycosylation is a crucial step in plant defense mechanisms, catalyzed by enzymes like SGD to produce reactive aglycones. Alternative splicing of the SGD gene in C. roseus leads to the shorter isoform shSGD, which disrupts SGD multimers and inhibits its activity, impacting downstream MIA synthesis. This pseudo-enzyme shSGD acts as a regulator of MIA biosynthesis by interfering with metabolic channeling and recruitment of enzymes.
Article
Plant Sciences
Vyoma Mistry, Pragya Tiwari, Paresh Patel, Gajendra Singh Vishwakarma, Geung-Joo Lee, Abhishek Sharma
Summary: Catharanthus roseus synthesizes bioactive therapeutic metabolites known as monoterpenoid indole alkaloids (MIAs), including anti-neoplastic vinblastine and vincristine, as well as antihypertensive ajmalicine. Biotechnological interventions have been used to enhance the production of MIAs in plant systems due to their low in planta concentration. Mutagenesis experiments using chemical and physical agents on C. roseus showed potential effects on the transcriptional modulation of MIA biosynthetic pathways in plants, leading to changes in plant morphology and increased content of specific bioactive compounds.
Article
Horticulture
Sima Sazegari, Ali Niazi, Farajollah Shahriari-Ahmadi, Alireza Afsharifar
Summary: In this study, CrMYC1 was cloned and overexpressed in Catharanthus roseus hairy roots. The overexpression of CrMYC1 significantly improved the accumulation of ajmalicine and catharanthine. These findings provide insight into the regulatory role of this understudied transcription factor in the TIA pathway and highlight further research goals.
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Joana G. Guedes, Rogerio Ribeiro, Ines Carqueijeiro, Ana Luisa Guimaraes, Claudia Bispo, John Archer, Herlander Azevedo, Nuno A. Fonseca, Mariana Sottomayor
Summary: The mesophyll idioblasts of Catharanthus roseus are a hotspot for alkaloid accumulation and have a distinctive transcriptomic profile associated with stress responses. The transcriptome of idioblasts may hold the key to understanding the MIA pathway and increasing the levels of anticancer drugs.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Kotaro Yamamoto, Dagny Grzech, Konstantinos Koudounas, Emily Amor Stander, Lorenzo Caputi, Tetsuro Mimura, Vincent Courdavault, Sarah E. O'Connor
Summary: Researchers developed an improved VIGS method and discovered a new biosynthetic enzyme, serpentine synthase (SS), in Catharanthus roseus, which produces beta-carboline alkaloids with strong blue autofluorescence and potential pharmacological activity.
Article
Agricultural Engineering
Neda Tariverdizadeh, Mehdi Mohebodini, Esmaeil Chamani, Asghar Ebadi
Summary: The study showed that induction of hairy roots from fenugreek cotyledon explants using different strains of Agrobacterium rhizogenes followed by treatment with zinc oxide nanoparticles can lead to higher levels of trigonelline. The best results were obtained with the ATCC11325 strain and Zn (2x) treatment, resulting in increased fresh and dry weight of hairy roots and higher trigonelline content compared to the control group.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Dylan Levac, Paulo Cazares Flores, Vincenzo De Luca
Summary: This study describes the identification and functional expression of a N-methyltransferase in Catharanthus roseus, providing detailed information about the pathway for the formation of anticancer alkaloids.
Article
Plant Sciences
Yuekai Su, Caicai Lin, Jin Zhang, Bei Hu, Jie Wang, Jingyu Li, Shiqi Wang, Ruihao Liu, Xia Li, Zhenqiao Song, Jianhua Wang
Summary: A novel one-step hairy root regeneration method was developed for Salvia miltiorrhiza, resulting in high tanshinone content in regenerated plants. This method provides a foundation for breeding S. miltiorrhiza and sustainable development of medicinal plant resources, serving as a useful reference for the application of other species.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Diksha Bagal, Aksar Ali Chowdhary, Shakti Mehrotra, Sonal Mishra, Sonica Rathore, Vikas Srivastava
Summary: Plants are important sources of secondary metabolites, but natural plant populations cannot meet the commercial demand due to low accumulation volume. Hairy root cultures induced by plant interaction with Rhizobium rhizogenes are a practical solution for producing valuable secondary metabolites at low cost and without seasonal variations. Metabolic engineering in hairy roots, including overexpression and silencing of genes and transcription factor overexpression, can enhance metabolite production. This review focuses on alkaloids and discusses future prospects for meeting production demands through metabolic engineering in hairy roots.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Rogelio Reyes-Perez, Maribel Herrera-Ruiz, Irene Perea-Arango, Fernando Martinez-Morales, Jose De Jesus Arellano Garcia, Pilar Nicasio-Torres
Summary: Sphaeralcea angustifolia, traditionally used in Mexican medicine, has anti-inflammatory and immunomodulatory activities attributed to compounds like scopoletin. Studies have shown that certain components of S. angustifolia possess anti-inflammatory and immunomodulatory activities, with transformed roots being proposed as producers of active compounds.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Review
Plant Sciences
Sunil Kumar, Bikarma Singh, Ramesh Singh
Summary: Catharanthus roseus is a traditional medicinal plant used in the treatment of cancer and diabetes, containing various compounds with pharmacological activities. Research has shown that extracts and compounds from this plant exhibit potential anticancer and antidiabetic properties, but caution is needed as toxicity can occur beyond a certain limit.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mahdi Yahyazadeh, Gerold Jerz, Peter Winterhalter, Dirk Selmar
Summary: Medicinal plants grown under stress conditions exhibit higher concentrations of specialized metabolites, potentially due to enhanced biosynthesis, although stress also reduces biomass gain. Study on Catharanthus roseus plants showed that alkaloid concentrations were markedly higher under drought stress, but overall alkaloid content per plant increased only in the first two weeks. High salt concentrations did not affect alkaloid concentrations but significantly decreased total alkaloid content, suggesting that both increased biosynthesis and reduced biomass contribute to stress-related concentration increase.
Article
Biochemistry & Molecular Biology
Dhammaprakash Pandhari Wankhede, Pallavi Singh, Monika Jaggi, Kudupudi Prabhakara Rao, Susheel Kumar Raina, Alok Krishna Sinha
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Vincent Ricigliano, Santosh Kumar, Scott Kinison, Christopher Brooks, S. Eric Nybo, Joe Chappell, Dianella G. Howarth
Article
Biochemistry & Molecular Biology
Santosh Kumar, Chase Kempinski, Xun Zhuang, Ayla Norris, Sibongile Mafu, Jiachen Zi, Stephen A. Bell, Stephen Eric Nybo, Scott E. Kinison, Zuodong Jiang, Sheba Goklany, Kristin B. Linscott, Xinlu Chen, Qidong Jia, Shoshana D. Brown, John L. Bowman, Patricia C. Babbitt, Reuben J. Peters, Feng Chen, Joe Chappell
Article
Microbiology
Carrie L. Shaffer, James A. D. Good, Santosh Kumar, K. Syam Krishnan, Jennifer A. Gaddy, John T. Loh, Joseph Chappell, Fredrik Almqvist, Timothy L. Cover, Maria Hadjifrangiskou
Article
Biotechnology & Applied Microbiology
Zuodong Jiang, Chase Kempinski, Santosh Kumar, Scott Kinison, Kristin Linscott, Eric Nybo, Sarah Janze, Connie Wood, Joe Chappell
PLANT BIOTECHNOLOGY JOURNAL
(2018)
Article
Multidisciplinary Sciences
Manoj Majee, Santosh Kumar, Praveen Kumar Kathare, Shuiqin Wu, Derek Gingerich, Nihar R. Nayak, Louai Salaita, Randy Dinkins, Kathleen Martin, Michael Goodin, Lynnette M. A. Dirk, Taylor D. Lloyd, Ling Zhu, Joseph Chappell, Arthur G. Hunt, Richard Vierstra, Enamul Huq, A. Bruce Downie
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Plant Sciences
Susheel Kumar Raina, Dhammaprakash Pandhari Wankhede, Monika Jaggi, Pallavi Singh, Siddhi Kashinath Jalmi, Badmi Raghuram, Arsheed Hussain Sheikh, Alok Krishna Sinha
Article
Biochemistry & Molecular Biology
Rekha Kushwaha, Taylor D. Lloyd, Kim R. Schaefermeyer, Santosh Kumar, Allan Bruce Downie
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2012)
Article
Plant Sciences
Santosh Kumar, Monika Jaggi, Jyoti Taneja, Alok Krishna Sinha
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2011)
Article
Plant Sciences
Santosh Kumar, Monika Jaggi, Alok Krishna Sinha
Article
Chemistry, Analytical
Zhentian Lei, Santosh Kumar, Saurav J. Sarma, Ritesh Kumar, Barbara W. Sumner, Minviluz G. Stacey, Lloyd W. Sumner
JOURNAL OF ANALYSIS AND TESTING
(2020)
Article
Biochemistry & Molecular Biology
Lynnette M. A. Dirk, Santosh Kumar, Manoj Majee, A. Bruce Downie
PLANT SIGNALING & BEHAVIOR
(2018)
Article
Biochemistry & Molecular Biology
Alok Krishna Sinha, Monika Jaggi, Badmi Raghuram, Narendra Tuteja
PLANT SIGNALING & BEHAVIOR
(2011)