Article
Biochemistry & Molecular Biology
Ting Yang, Yi Shi, Qian Yang, Yu Xin, Zhenghua Gu, Liang Zhang
Summary: The study investigated the nitrogen removal characteristics of a newly isolated aerobic denitrifier Bacillus subtilis JD-014, showing efficient denitrification performance within a certain range of nitrate concentration when using sodium succinate as the carbon source. Transcriptome analysis revealed that up-regulated differentially expressed genes were associated with cell motility, carbohydrate metabolism, and energy metabolism. Additionally, a regulator gene nirsir was identified as participating in the nitrogen removal process within JD-014.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Horticulture
Fangying Qiu, Wenhuan Liu, Lang Chen, Ya Wang, Yanyan Ma, Qiang Lyu, Shilai Yi, Rangjin Xie, Yongqiang Zheng
Summary: This study evaluated the effects of liquid Bacillus subtilis commercial microbial fertilizer (BCMF) on fruit quality and plant nutrient status of fertigated citrus groves. Results showed that BCMF applications had positive correlation with fruit quality and leaf nutrient concentrations, with different effects observed between CF100 + BCMF and CF50 + BCMF treatments. The application of BCMF contributed to reducing fertilizer input and exerting positive effects on fruit quality in fertigated Tarocco blood orange groves.
SCIENTIA HORTICULTURAE
(2021)
Article
Biochemistry & Molecular Biology
Aniruddha Tewary, Ranjit Kumar Prajapati, Jayanta Mukhopadhyay
Summary: The C-terminal domain of the α subunit of RNA polymerase is targeted by transcriptional regulators in the activation of transcription. In bacteria, the regulator binds to DNA at different sites and interacts with the α CTD to stabilize the RNA polymerase at the promoter or form a pre-recruitment complex. In B. subtilis, the binding of RNA polymerase at the promoter stabilizes the transcriptional regulator and facilitates open complex formation. Two residues of the α CTD are essential for this process.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xiumin Ding, Zhiming Zheng, Genhai Zhao, Li Wang, Han Wang, Qiang Yang, Mengxue Zhang, Luyao Li, Peng Wang
Summary: In this study, a synthetic strain with high efficiency, low carbon, and cofactor recycling for MK-7 synthesis was constructed using synthetic biology methods. The synthetic redesign of the synthesis pathway and optimization of material and energy metabolism improved the production of MK-7. The strategy used in this study can be applied to the construction of high-efficiency synthesis platforms for other terpenoids, paving the way for large-scale production of MK-7 and other valuable terpenoids.
MICROBIAL CELL FACTORIES
(2022)
Article
Microbiology
Rania Hammami, Maroua Oueslati, Marwa Smiri, Souhaila Nefzi, Mustapha Ruissi, Francesca Comitini, Gianfranco Romanazzi, Santa Olga Cacciola, Najla Sadfi Zouaoui
Summary: In this study, yeast and bacterial isolates with antagonistic activity against Penicillium digitatum and P. italicum on citrus fruits were screened and identified. These isolates significantly reduced the incidence of green and blue mold caused by these pathogens on citrus fruits, and effectively prevented fungal infection during low-temperature storage. Moreover, they did not have any negative effect on fruit quality and shelf life. These selected isolates have multiple modes of action and show promise as biocontrol agents for postharvest green and blue molds of citrus fruits.
Article
Biotechnology & Applied Microbiology
Qian Pi, Zhubing Zhu, Lei Tang
Summary: A new superfamily of heme-containing peroxidases, DyPs, has been discovered to efficiently decolorize various dyes, especially anthraquinone dyes. Using TfuDyP, it was found that the attack site on RB19 was identified and the incomplete degradation products of RB19 inhibited the growth of Bacillus subtilis. These findings provide a novel understanding of DyPs catalysis to anthraquinone dyes.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Bram Van den Bergh, Hannah Schramke, Joran Elie Michiels, Tom E. P. Kimkes, Jakub Leszek Radzikowski, Johannes Schimpf, Silke R. Vedelaar, Sabrina Burschel, Liselot Dewachter, Nikola Loncar, Alexander Schmidt, Tim Meijer, Maarten Fauvart, Thorsten Friedrich, Jan Michiels, Matthias Heinemann
Summary: Antibiotic persisters are phenotypic variants within an isogenic bacterial population that are transiently tolerant to antibiotic treatment. This study provides evidence that cytoplasmic acidification, amplified by a compromised respiratory complex I, can act as a signaling hub for perturbed metabolic homeostasis in antibiotic persisters.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Tingting Chen, Zhuzhu Zhang, Wenzhi Li, Jia Chen, Xuetang Chen, Bince Wang, Jiling Ma, Yunyun Dai, Haixia Ding, Weizhen Wang, Youhua Long
Summary: This study isolated a strain of Bacillus subtilis, named CTXW 7-6-2, from healthy kiwifruit tissue, which showed inhibitory effects on pathogenic fungi causing kiwifruit soft rot. The study identified the gene mechanisms and volatile compounds associated with the antifungal activity of CTXW 7-6-2, providing potential insights for the development of biocontrol strategies in the kiwifruit industry.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Katherine J. Wozniak, Lyle A. Simmons
Summary: Hydroxyurea (HU) functions as a ribonucleotide reductase (RNR) inhibitor to stall DNA replication by depleting deoxyribonucleoside triphosphate (dNTP) pools. In Bacillus subtilis, HU challenge leads to cell elongation, slowed replication fork progression, metabolic and DNA damage responses. Overexpression of the RNR genes nrdEF can suppress growth interference caused by HU.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Energy & Fuels
Mehlika Ozzeybek, Deniz Cekmecelioglu
Summary: This study investigates the use of mixed feedstock, including apple pomace, orange peels, and hazelnut shells, to enhance the co-production of bacterial pectinase and cellulase enzymes. The results show a 40% increase in pectinase production but no change in cellulase production compared to the use of hazelnut shell hydrolysate medium. Hazelnut shells also give slightly higher pectinase production than apple pomace and orange peel media.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biochemistry & Molecular Biology
Hye Yeon Cho, Mi Sun Nam, Ho Jeong Hong, Wan Seok Song, Sung-il Yoon
Summary: NAD(H)/NADP(H)-dependent aldehyde/alcohol oxidoreductase (AAOR) plays a crucial role in reducing aldehydes or oxidizing alcohols in various cellular processes. A unique AAOR, Bacillus subtilis YugJ, has been identified that specifically reduces furan aldehyde HMF in an NADPH- and Ni2+-dependent manner. The structural analyses of YugJ revealed key interactions with NADP and Ni2+, providing insights into its substrate-binding mode.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Colin J. Comerci, Alan L. Gillman, Leticia Galera-Laporta, Edgar Gutierrez, Alex Groisman, Joseph W. Larkin, Jordi Garcia-Ojalvo, Gurol M. Suel
Summary: This study developed an experimental platform combining a microfluidic chip with a multielectrode array to enable localized electrochemical stimulation of bacterial biofilms and simultaneous measurement of physiological response. The findings show that the stimulation of an electrode can selectively promote the proliferation of a specific bacterial cell type, revealing the potential control of biofilm composition and development through electronic interface.
Article
Microbiology
Dai Zhang, Ran Qiang, Jing Zhao, Jinglin Zhang, Jianing Cheng, Dongmei Zhao, Yaning Fan, Zhihui Yang, Jiehua Zhu
Summary: This study investigated the antagonistic mechanisms of the volatile organic compound 6-methyl-2-heptanone produced by Bacillus against Alternaria solani. The compound exhibited strong inhibitory effects on mycelial growth and conidial germination of A. solani. It also caused structural abnormalities in hyphal cells and down-regulated the expression of pathogenic genes in A. solani. These findings provide insights into the mechanisms of VOCs against A. solani.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Linxia Liu, Jinlong Li, Yuanming Gai, Zhizhong Tian, Yanyan Wang, Tenghe Wang, Pi Liu, Qianqian Yuan, Hongwu Ma, Sang Yup Lee, Dawei Zhang
Summary: Vitamin B6 is a vital nutrient widely used in medicine, food, animal feed, and cosmetics industries. Researchers have developed an engineered strain of Escherichia coli to produce vitamin B6 more efficiently and affordably. By optimizing the metabolic pathway and enzyme efficiency, the final strain achieved a high productivity of 1.4 g/L of vitamin B6 through fed-batch fermentation. These strategies are valuable for developing microbial strains for producing vitamins and other bioproducts with low metabolic fluxes.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Hannah Jacob, Hao Geng, Dasvit Shetty, Nathan Halow, Linda J. Kenney, Michiko M. Nakano
Summary: This study investigated the mechanism of ResD-dependent transcription activation of the nasD operon and identified the important roles of ResD-RNAP and ResD-DNA interactions in nasD transcription. The interaction between alpha CTD and ResD at the promoter-distal site was proposed to be essential for stimulating nasD transcription, and the absence of the -35 element might be compensated by interactions between sigma(A) and alpha CTD and between alpha CTD and ResD at the promoter-proximal ResD-binding subsite.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Chemistry, Applied
Barbara Hufnagel, Verena Muellner, Katharina Hlatky, Claudia Tallian, Robert Vielnascher, Georg M. Guebitz, Michael Wirth, Franz Gabor
Summary: This study explored a new preparation technique for pellets made from acetylated inulin to achieve specific intestinal delivery of 5-aminosalicylic acid. The acetylated inulins were insoluble in water with increased hydrophobicity, and showed high drug loading capacity and release rate, offering a promising new strategy for the treatment of Crohn's disease and ulcerative colitis.
CARBOHYDRATE POLYMERS
(2021)
Article
Environmental Sciences
Klemens Kremser, Sophie Thallner, Dorina Strbik, Sabine Spiess, Jiri Kucera, Tomas Vaculovic, Dalibor Vsiansky, Marianne Haberbauer, Martin Mandl, Georg M. Guebitz
Summary: This study investigated the use of acidophilic bioleaching bacteria to recover valuable metals from incineration residues, with A. ferrooxidans showing high extraction efficiency for Zinc, Cobalt, Copper, and Manganese. Analysis using ICP-MS revealed economically attractive concentrations of various metals in the ashes and slags. The biological approach showed advantages over conventional processes in terms of acid usage, temperature requirements, and emission of toxic gases.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Engineering, Environmental
Klemens Kremser, Patrick Gerl, Alessandro Pellis, Georg M. Guebitz
Summary: This study explored the use of three bioleaching bacteria to selectively leach aluminum from the epoxy layer, with leaching efficiencies reaching around 92% after three weeks of incubation. Surface characterization showed that the nature of the epoxy resin remained unchanged after bioleaching application, allowing for recycling. Selective precipitation of dissolved aluminum from lixiviants at pH = 6.5 resulted in aluminum hydroxide precipitation efficiencies of almost 100%, demonstrating the significant potential of acidophilic bacteria in the separation and recycling of multi-layer materials.
Article
Chemistry, Multidisciplinary
Romain Schieber, Yago Raymond, Cristina Caparros, Jordi Bou, Enrique Herrero Acero, Georg M. Guebitz, Cristina Canal, Marta Pegueroles
Summary: The study investigated the effect of different functionalization strategies on PLLA surfaces to enhance endothelial cell adhesion and the fabrication of 3D printed BRS. Surface functionalization with oxygen plasma, sodium hydroxide etching, or cutinase enzyme hydrolysis increased hydroxyl and carboxyl groups, improving endothelial cells adhesion without affecting degradation. 3D printed PLLA BRS stents were successfully produced using the solvent-cast direct writing technique.
APPLIED SCIENCES-BASEL
(2021)
Review
Biotechnology & Applied Microbiology
Lucie Lalanne, Gibson S. Nyanhongo, Georg M. Guebitz, Alessandro Pellis
Summary: Of the 25 million tons of plastic waste produced every year in Europe, 40% are not reused or recycled, contributing to environmental pollution, a major challenge of the 21st century; Bio-based monomers, derived from renewable raw materials, are a possible solution for replacing oil-derived monomers, with furan derivatives having great potential for the synthesis of biobased polyesters, polyamides and their copolymers.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biotechnology & Applied Microbiology
Oskar Haske-Cornelius, Sonja Gierlinger, Robert Vielnascher, Wolfgang Gabauer, Katharina Prall, Alessandro Pellis, Georg M. Guebitz
Summary: This study investigated the cellulases-catalyzed hydrolysis of paper slurries as carbon source in combination with digestate for the cultivation of Chlorella sorokiniana. The most efficient cultivation converted glucose into biomass, but high concentrations of glucose may be inhibited by a decrease in pH.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Materials Science, Paper & Wood
Martin Nagl, Oskar Haske-Cornelius, Lukas Skopek, Alessandro Pellis, Wolfgang Bauer, Gibson Stephen Nyanhongo, Georg Guebitz
Summary: Paper, with an annual production of over 400 million tons, is the main product of the largest industrially implemented biorefinery process. Enzymes have been used to refine pulp, significantly reducing energy consumption. Different commercial refining enzyme formulations showed varying activities on model substrates, indicating the importance of understanding individual enzyme mechanisms.
Article
Biochemistry & Molecular Biology
Filippo Fabbri, Federico A. Bertolini, Georg M. Guebitz, Alessandro Pellis
Summary: The study focused on investigating the activity of different hydrolases immobilized on polypropylene beads in short-esters and polyesters synthesis. A full-factorial design of experiments was developed to analyze the thermostability and selectivity of the enzymes towards alcohols and acids with different chain lengths. The enzymes displayed varying temperature optima and substrate preferences, which influenced the polymerization of esters and the molecular masses of the resulting polyesters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Jenny Arnling Baath, Vera Novy, Leonor Carneiro, Georg M. Guebitz, Lisbeth Olsson, Peter Westh, Doris Ribitsch
Summary: This study rigorously characterized two almost identical cutinases and demonstrated that even minor sequence differences in cutinases can drastically affect their substrate binding, substrate specificity, and catalytic efficiency.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Materials Science, Paper & Wood
Martin Nagl, Oskar Haske-Cornelius, Lukas Skopek, Florian Bausch, Alessandro Pellis, Wolfgang Bauer, Gibson S. Nyanhongo, Georg M. Guebitz
Summary: Endoglucanases are potential solutions for reducing energy consumption in the pulp and paper industry, but the performance varies due to the different enzyme formulations. This study investigated the effect of four endoglucanases on softwood and hardwood pulp, revealing that degradation of amorphous regions is beneficial for the refining process.
Article
Biochemistry & Molecular Biology
Franziska Steger, Johanna Reich, Werner Fuchs, Simon K. -M. R. Rittmann, Georg M. Guebitz, Doris Ribitsch, Guenther Bochmann
Summary: Strategies for depleting carbon dioxide (CO2) from flue gases are urgently needed, and carbonic anhydrases (CAs) can contribute to solving this problem. This current study examines four recombinantly produced CAs from different organisms, namely CAs from Acetobacterium woodii (AwCA or CynT), Persephonella marina (PmCA), Methanobacterium thermoautotrophicum (MtaCA or Cab), and Sulphurihydrogenibium yellowstonense (SspCA). The highest expression yields and activities were found for AwCA and PmCA. AwCA showed high stability in a mesophilic temperature range, while PmCA proved to be exceptionally thermostable. These results indicate the potential to utilize CAs from anaerobic microorganisms for CO2 sequestration applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Raphaela Hellmayr, Sabrina Bischof, Jasmin Wuehl, Georg M. Guebitz, Gibson S. Nyanhongo, Nikolaus Schwaiger, Falk Liebner, Rupert Wimmer
Summary: This study investigates the effect of enzymatic polymerization of lignosulfonate on the formulation of a lignosulfonate-based adhesive. The results show that the enzyme-polymerized lignosulfonate adhesive has similar mechanical properties to standard carpenter's glue, but with different failure modes at the bonding area. The pre-polymerization time has an impact on the viscosity and tensile shear strength of the adhesive.
Article
Polymer Science
Federico Di Bisceglie, Felice Quartinello, Robert Vielnascher, Georg M. Guebitz, Alessandro Pellis
Summary: Polyurethanes are widely used synthetic polymers in Europe, and their recycling poses a major challenge for the industry. This study investigated the enzymatic hydrolysis of a polyurethane-polyester copolymer to achieve decomposition under milder conditions. The results showed that the enzyme effectively hydrolysed the polymer and reduced its molecular weight, indicating the potential for environmentally friendly recycling strategies.
Article
Chemistry, Multidisciplinary
Federico A. Bertolini, Michelina Soccio, Simone Weinberger, Giulia Guidotti, Massimo Gazzano, Georg M. Guebitz, Nadia Lotti, Alessandro Pellis
Summary: In the study, thiophene-based polymers (TBPs) were synthesized and enzymatic hydrolysis experiments were conducted using cutinase enzymes from Thermobifida cellulosilytica, showing promising degradation results for PBTF, PPeTF, and PHTF with the release of TPCA. The research indicates that these thermostable cutinases could be effective biocatalysts for environmentally friendly degradation of TPCA-based polyesters, potentially facilitating sustainable recycling of plastic waste through resynthesis processes.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Renate Weiss, Sebastian Gritsch, Guenter Brader, Branislav Nikolic, Marc Spiller, Julia Santolin, Hedda K. Weber, Nikolaus Schwaiger, Sylvain Pluchon, Kristin Dietel, Georg Guebitz, Gibson Nyanhongo
Summary: Bioactive coatings based on lignosulfonate and developed using laccase as a biocatalyst were successfully created to improve soil for lawns. The addition of glycerol, xylitol, and sorbitol as plasticizers helped reduce brittleness in the coatings, while thermal enzyme inactivation at 100 degrees Celsius enabled stable coating production. The incorporation of beneficial microorganisms and the ability to reduce CO2 emissions make these coatings a promising alternative to fossil-based options.