Structural and Functional Insights to Ubiquitin-Like Protein Conjugation

Activation of UbcH5c∼Ub Is the Result of a Shift in Interdomain Motions of the Conjugate Bound to U-Box E3 Ligase E4B

(2013) Sarah E. Soss et al.   BIOCHEMISTRY

Macromolecular juggling by ubiquitylation enzymes

(2013) Sonja Lorenz et al.   BMC BIOLOGY

Molecular and structural insight into lysine selection on substrate and ubiquitin lysine 48 by the ubiquitin-conjugating enzyme Cdc34

(2013) Randy Suryadinata et al.   CELL CYCLE

Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine

(2013) Ranabir Das et al.   EMBO JOURNAL

Structure of the human Parkin ligase domain in an autoinhibited state

(2013) Tobias Wauer et al.   EMBO JOURNAL

Tripartite Motif Ligases Catalyze Polyubiquitin Chain Formation through a Cooperative Allosteric Mechanism

(2013) Frederick C. Streich et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

E6AP/UBE3A Ubiquitin Ligase Harbors Two E2∼ubiquitin Binding Sites

(2013) Virginia P. Ronchi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

PINK1 drives Parkin self-association and HECT-like E3 activity upstream of mitochondrial binding

(2013) Michael Lazarou et al.   JOURNAL OF CELL BIOLOGY

Structure of a Ubiquitin E1-E2 Complex: Insights to E1-E2 Thioester Transfer

(2013) Shaun K. Olsen et al.   MOLECULAR CELL

Ubc9 Sumoylation Controls SUMO Chain Formation and Meiotic Synapsis in Saccharomyces cerevisiae

(2013) Helene Klug et al.   MOLECULAR CELL

A Structurally Unique E2-Binding Domain Activates Ubiquitination by the ERAD E2, Ubc7p, through Multiple Mechanisms

(2013) Meredith B. Metzger et al.   MOLECULAR CELL

A conserved asparagine has a structural role in ubiquitin-conjugating enzymes

(2013) Christopher E Berndsen et al.   Nature Chemical Biology

Structure of a ubiquitin-loaded HECT ligase reveals the molecular basis for catalytic priming

(2013) Elena Maspero et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Essentiality of a non-RING element in priming donor ubiquitin for catalysis by a monomeric E3

(2013) Hao Dou et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of Parkin Reveals Mechanisms for Ubiquitin Ligase Activation

(2013) J.-F. Trempe et al.   SCIENCE

Structure of HHARI, a RING-IBR-RING Ubiquitin Ligase: Autoinhibition of an Ariadne-Family E3 and Insights into Ligation Mechanism

(2013) David M. Duda et al.   STRUCTURE

A molecular explanation for the recessive nature of parkin-linked Parkinson’s disease

(2013) Donald E. Spratt et al.   Nature Communications

Structure and function of Parkin E3 ubiquitin ligase reveals aspects of RING and HECT ligases

(2013) B.E. Riley et al.   Nature Communications

A disulphide bond in the E2 enzyme Pex4p modulates ubiquitin-conjugating activity

(2013) Chris Williams et al.   Scientific Reports

Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3

(2013) Hari B Kamadurai et al.   eLife

Structural Insights into the Conformation and Oligomerization of E2∼Ubiquitin Conjugates

(2012) Richard C. Page et al.   BIOCHEMISTRY

LUBAC synthesizes linear ubiquitin chains via a thioester intermediate

(2012) Benjamin Stieglitz et al.   EMBO REPORTS

Mechanistic Studies on Activation of Ubiquitin and Di-ubiquitin-like Protein, FAT10, by Ubiquitin-like Modifier Activating Enzyme 6, Uba6

(2012) James M. Gavin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Selective Recruitment of an E2∼Ubiquitin Complex by an E3 Ubiquitin Ligase

(2012) Donald E. Spratt et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of an E3:E2∼Ub Complex Reveals an Allosteric Mechanism Shared among RING/U-box Ligases

(2012) Jonathan N. Pruneda et al.   MOLECULAR CELL

The RanBP2/RanGAP1∗SUMO1/Ubc9 Complex Is a Multisubunit SUMO E3 Ligase

(2012) Andreas Werner et al.   MOLECULAR CELL

Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis

(2012) Anna Plechanovová et al.   NATURE

Noncanonical recognition and UBL loading of distinct E2s by autophagy-essential Atg7

(2012) Masaya Yamaguchi et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

BIRC7–E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer

(2012) Hao Dou et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7–Atg3 and Atg7–Atg10 structures

(2012) Stephen E Kaiser et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl

(2012) Hao Dou et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural Insights into Atg10-Mediated Formation of the Autophagy-Essential Atg12-Atg5 Conjugate

(2012) Masaya Yamaguchi et al.   STRUCTURE

Structural Conservation of Distinctive N-terminal Acetylation-Dependent Interactions across a Family of Mammalian NEDD8 Ligation Enzymes

(2012) Julie K. Monda et al.   STRUCTURE

The Cullin-RING Ubiquitin-Protein Ligases

(2011) Zhihua Hua et al.   Annual Review of Plant Biology

Ubiquitin in Motion: Structural Studies of the Ubiquitin-Conjugating Enzyme∼Ubiquitin Conjugate

(2011) Jonathan N. Pruneda et al.   BIOCHEMISTRY

The Mechanism of Linkage-Specific Ubiquitin Chain Elongation by a Single-Subunit E2

(2011) Katherine E. Wickliffe et al.   CELL

An Allosteric Inhibitor of the Human Cdc34 Ubiquitin-Conjugating Enzyme

(2011) Derek F. Ceccarelli et al.   CELL

Structural regulation of cullin-RING ubiquitin ligase complexes

(2011) David M Duda et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Autoregulation of Parkin activity through its ubiquitin-like domain

(2011) Viduth K Chaugule et al.   EMBO JOURNAL

Structure of the HECT:ubiquitin complex and its role in ubiquitin chain elongation

(2011) Elena Maspero et al.   EMBO REPORTS

Structure and function of a HECT domain ubiquitin-binding site

(2011) Hyung Cheol Kim et al.   EMBO REPORTS

Flexible Cullins in Cullin-RING E3 Ligases Allosterically Regulate Ubiquitination

(2011) Jin Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

E1-E2 Interactions in Ubiquitin and Nedd8 Ligation Pathways

(2011) Zeynep Tokgöz et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Determinants of Small Ubiquitin-like Modifier 1 (SUMO1) Protein Specificity, E3 Ligase, and SUMO-RanGAP1 Binding Activities of Nucleoporin RanBP2

(2011) Jaclyn R. Gareau et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Neddylation-Induced Conformational Control Regulates Cullin RING Ligase Activity In Vivo

(2011) Boon Kim Boh et al.   JOURNAL OF MOLECULAR BIOLOGY

Regulation of Ubiquitin Chain Initiation to Control the Timing of Substrate Degradation

(2011) Adam Williamson et al.   MOLECULAR CELL

Essential Role for Ubiquitin-Ubiquitin-Conjugating Enzyme Interaction in Ubiquitin Discharge from Cdc34 to Substrate

(2011) Anjanabha Saha et al.   MOLECULAR CELL

Atg8 Transfer from Atg7 to Atg3: A Distinctive E1-E2 Architecture and Mechanism in the Autophagy Pathway

(2011) Asad M. Taherbhoy et al.   MOLECULAR CELL

Structural Basis of Atg8 Activation by a Homodimeric E1, Atg7

(2011) Nobuo N. Noda et al.   MOLECULAR CELL

UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids

(2011) Dawn M. Wenzel et al.   NATURE

A RING E3–substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases

(2011) Matthew F Calabrese et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Insights into noncanonical E1 enzyme activation from the structure of autophagic E1 Atg7 with Atg8

(2011) Seung Beom Hong et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The Relative Rates of Thiol–Thioester Exchange and Hydrolysis for Alkyl and Aryl Thioalkanoates in Water

(2011) Paul J. Bracher et al.   ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES

N-Terminal Acetylation Acts as an Avidity Enhancer Within an Interconnected Multiprotein Complex

(2011) D. C. Scott et al.   SCIENCE

Structural assembly of cullin-RING ubiquitin ligase complexes

(2010) Erik S Zimmerman et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Ser120of Ubc2/Rad6 Regulates Ubiquitin-dependent N-end Rule Targeting by E3α/Ubr1

(2010) Brajesh Kumar et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Designed Semisynthetic Protein Inhibitors of Ub/Ubl E1 Activating Enzymes

(2010) Xuequan Lu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Molecular Basis for Lysine Specificity in the Yeast Ubiquitin-Conjugating Enzyme Cdc34

(2010) M. Sadowski et al.   MOLECULAR AND CELLULAR BIOLOGY

Catalysis of Lysine 48-Specific Ubiquitin Chain Assembly by Residues in E2 and Ubiquitin

(2010) Monica C. Rodrigo-Brenni et al.   MOLECULAR CELL

A Dual E3 Mechanism for Rub1 Ligation to Cdc53

(2010) Daniel C. Scott et al.   MOLECULAR CELL

Substrate-Assisted Inhibition of Ubiquitin-like Protein-Activating Enzymes: The NEDD8 E1 Inhibitor MLN4924 Forms a NEDD8-AMP Mimetic In Situ

(2010) James E. Brownell et al.   MOLECULAR CELL

Active site remodelling accompanies thioester bond formation in the SUMO E1

(2010) Shaun K. Olsen et al.   NATURE

The SUMO pathway: emerging mechanisms that shape specificity, conjugation and recognition

(2010) Jaclyn R. Gareau et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Molecular Basis for the Association of Human E4B U Box Ubiquitin Ligase with E2-Conjugating Enzymes UbcH5c and Ubc4

(2010) Robert C. Benirschke et al.   STRUCTURE

RING Domain E3 Ubiquitin Ligases

(2009) Raymond J. Deshaies et al.   Annual Review of Biochemistry

Regulation of cullin-RING E3 ubiquitin-ligases by neddylation and dimerization

(2009) J. Merlet et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Cullin neddylation and substrate-adaptors counteract SCF inhibition by the CAND1-like protein Lag2 in Saccharomyces cerevisiae

(2009) Edyta Siergiejuk et al.   EMBO JOURNAL

A longevity protein, Lag2, interacts with SCF complex and regulates SCF function

(2009) Yuan Liu et al.   EMBO JOURNAL

Polyubiquitination by HECT E3s and the Determinants of Chain Type Specificity

(2009) H. C. Kim et al.   MOLECULAR AND CELLULAR BIOLOGY

Protection from Isopeptidase-Mediated Deconjugation Regulates Paralog-Selective Sumoylation of RanGAP1

(2009) Shanshan Zhu et al.   MOLECULAR CELL

Structure of the Siz/PIAS SUMO E3 Ligase Siz1 and Determinants Required for SUMO Modification of PCNA

(2009) Ali A. Yunus et al.   MOLECULAR CELL

Insights into Ubiquitin Transfer Cascades from a Structure of a UbcH5B∼Ubiquitin-HECTNEDD4L Complex

(2009) Hari B. Kamadurai et al.   MOLECULAR CELL

Allosteric Activation of E2-RING Finger-Mediated Ubiquitylation by a Structurally Defined Specific E2-Binding Region of gp78

(2009) Ranabir Das et al.   MOLECULAR CELL

Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways

(2009) Brenda A. Schulman et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

E2 interaction and dimerization in the crystal structure of TRAF6

(2009) Qian Yin et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural Insights into E1-Catalyzed Ubiquitin Activation and Transfer to Conjugating Enzymes

(2008) Imsang Lee et al.   CELL

Structural Insights into NEDD8 Activation of Cullin-RING Ligases: Conformational Control of Conjugation

(2008) David M. Duda et al.   CELL

Structure of the MDM2/MDMX RING domain heterodimer reveals dimerization is required for their ubiquitylation in trans

(2008) K Linke et al.   CELL DEATH AND DIFFERENTIATION

The Atg8 and Atg12 ubiquitin-like conjugation systems in macroautophagy. ‘Protein Modifications: Beyond the Usual Suspects’ Review Series

(2008) Jiefei Geng et al.   EMBO REPORTS

Structures of the cIAP2 RING Domain Reveal Conformational Changes Associated with Ubiquitin-conjugating Enzyme (E2) Recruitment

(2008) Peter D. Mace et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Dcn1 Functions as a Scaffold-Type E3 Ligase for Cullin Neddylation

(2008) Thimo Kurz et al.   MOLECULAR CELL

Multimodal Activation of the Ubiquitin Ligase SCF by Nedd8 Conjugation

(2008) Anjanabha Saha et al.   MOLECULAR CELL

Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail

(2008) K. Yamoah et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA