Review
Pharmacology & Pharmacy
Yan Yan, Ming Li, Jie Lin, Yanan Ji, Kexin Wang, Dajun Yan, Yuntian Shen, Wei Wang, Zhongwei Huang, Haiyan Jiang, Hualin Sun, Lei Qi
Summary: This review summarizes the role of AMPK in regulating mitochondrial function and its impact on skeletal muscle metabolism and health.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemical Research Methods
Gabriel Elizalde, Alma Zuniga Munoz, Albert E. Almada
Summary: This article presents a protocol for isolating mouse muscle stem cells (MuSCs) using a two-step enzymatic and mechanical dissociation method coupled with fluorescence-activated cell sorting (FACS). The FACS-isolated MuSCs can be utilized for various downstream applications, including cell culture, cell transduction, immunofluorescence, and gene expression assays.
Article
Chemistry, Multidisciplinary
Ngoc Duc Vo, Anh Thi Van Nguyen, Hoi Thi Le, Nam Hoang Nguyen, Huong Thi Thu Pham
Summary: The study developed an alternative method, the MACS-Countess system, for CD4(+) T cell counting, which combines magnetic activated cell sorting with an automated cell counting system. Compared to the FACS gold standard, the MACS-Countess system showed a mean bias of 3.5% with a narrow limit of agreement, making it a reliable and cost-effective option for laboratories in limited resource regions.
APPLIED SCIENCES-BASEL
(2021)
Article
Food Science & Technology
Wen-Juan Song, Pei -Pei Liu, Zi-Qing Meng, Yan-Yan Zheng, Guang-Hong Zhou, Hui-Xia Li, Shi-Jie Ding
Summary: Cultured fat, an essential component in cultured meat production, shows promising similarity in fatty acid composition with authentic adipose tissue. Adipose progenitor cells (APCs) are identified as a potential candidate for cultured fat production, demonstrating high efficiency proliferation and differentiation characteristics.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Computer Science, Interdisciplinary Applications
Joseph Crutison, Thomas Royston
Summary: The combination of finite-element models with medical imaging has greatly contributed to our understanding of tissue mechanics. Diffusion tensor imaging has particularly aided in modeling brain injuries and other biological systems and diagnostic techniques can benefit from this approach. Dynamic elastography is an imaging technique that links contrast to the mechanical properties of tissue and can be improved by using magnetic resonance-based diffusion tensor (DT) imaging to generate subject-specific finite-element (FE) models. This work improves the accuracy of muscle elastic and viscous moduli estimates.
ENGINEERING WITH COMPUTERS
(2022)
Review
Immunology
Chenjie Fei, Li Nie, Jianhua Zhang, Jiong Chen
Summary: Teleost fish possess a complex assortment of highly specialized immune cells capable of unleashing immune responses, including memory lymphocytes. Specific roles of fish immune cells depend on precise separation of cell types, which may require monoclonal antibodies. Emerging technologies such as fluorescence-activated cell sorting and droplet-based microfluidics show potential in identifying antigen-specific B lymphocytes for fish immunology studies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Sebastian Mathes, Alexandra Fahrner, Umesh Ghoshdastider, Hannes A. Ruediger, Michael Leunig, Christian Wolfrum, Jan Kruetzfeldt
Summary: The research indicates that FGF-2 plays a dual role in promoting muscle growth and intramuscular adipogenesis, and activates the miR29a/SPARC axis to regulate differentiation of fibro/adipogenic progenitors and intramuscular adipose tissue formation in aged skeletal muscle.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Deirdre D. Scripture-Adams, Kevin N. Chesmore, Florian Barthelemy, Richard T. Wang, Shirley Nieves-Rodriguez, Derek W. Wang, Ekaterina Mokhonova, Emilie D. Douine, Jijun Wan, Isaiah Little, Laura N. Rabichow, Stanley F. Nelson, M. Carrie Miceli
Summary: This study develops a method to isolate and sequence individual nuclei from frozen muscle, allowing for the exploration of cellular and transcriptomic mechanisms in Duchenne muscular dystrophy. The findings reveal the effects of exon skipping therapy on muscle nuclei and demonstrate partial rescue of muscle fibers, expansion of myeloid population, recovery of M2 macrophages, and repression of inflammatory fibroblasts.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jacqueline A. Larouche, Paula M. Fraczek, Sarah J. Kurpiers, Benjamin A. Yang, Carol Davis, Jesus A. Castor-Macias, Kaitlyn Sabin, Shannon Anderson, Julia Harrer, Matthew Hall, Susan V. Brooks, Young C. Jang, Nick Willett, Lonnie D. Shea, Carlos A. Aguilar
Summary: This study compares the cellular and molecular mechanisms of fibrotic degeneration or regeneration of skeletal muscle (SkM) following volumetric muscle loss (VML) injuries. The researchers found that degenerative VML injuries led to increased infiltration of natural killer (NK) cells and persistent presence of neutrophils. The persistent infiltration of neutrophils impaired the regenerative function of muscle stem cells, which can be alleviated by blocking TGF beta signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Anirban Roy, Meiricris Tomaz da Silva, Raksha Bhat, Kyle R. Bohnert, Takao Iwawaki, Ashok Kumar
Summary: IRE1 alpha, along with its downstream target XBP1, play a crucial role in regulating skeletal muscle regeneration by promoting proliferation of satellite cells. The ablation of IRE1 alpha diminishes Notch signaling and canonical NF-kappa B pathway, leading to reduced satellite cell abundance and impaired muscle regeneration in both normal and Duchenne muscular dystrophy models.
Article
Biochemistry & Molecular Biology
Baojun Sun, Hitomi Maruta, Yun Ma, Hiromi Yamashita
Summary: Taurine is an abundant free amino acid in mammalian tissues that plays a role in skeletal muscle function and exercise capacity. This study investigated the mechanism of taurine function in skeletal muscles by examining the effects of short-term administration of taurine on rats and L6 cells. The results suggest that taurine modulates skeletal muscle function by activating AMP-activated protein kinase through the calcium signaling pathway, leading to the expression of genes and proteins associated with mitochondrial and respiratory metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Alexie A. Larson, Ahmed S. Shams, Shawna L. McMillin, Brian P. Sullivan, Cha Vue, Zachery A. Roloff, Eric Batchelor, Michael Kyba, Dawn A. Lowe
Summary: The study found that the size of the satellite cell pool is reduced in estradiol (E-2)-deficient female mice and humans. By using a combination of in vivo and in vitro approaches, they identified mechanisms whereby E-2 deficiency impairs satellite cell maintenance.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Review
Cell Biology
Magdaleena Naemi Mbadhi, Jun-ming Tang, Jing-xuan Zhang
Summary: Satellite stem cells with high regenerative capacity are ideal for treating muscular diseases. The regulation of myogenic regulatory factors plays a crucial role in the differentiation of satellite stem cells into skeletal muscles. While progress has been made in using HDACi, further research is needed on histone methylation modulations and lncRNA.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Alexander M. Morch, Falk Schneider, Edward Jenkins, Ana Mafalda Santos, Scott E. Fraser, Simon J. Davis, Michael L. Dustin
Summary: The sensitivity of the α(3) T cell receptor (TCR) is enhanced by the coreceptors CD4 and CD8 α(3). We found that the interaction between CD4 and Lck is stoichiometric and the interaction between CD8 α(3) and Lck is substantial. These findings help settle the question of kinase occupancy and suggest that the binding advantages that CD4 has over CD8 could be important when Lck levels are limiting.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Materials Science, Multidisciplinary
Matthew Lindley, Julia Gala de Pablo, Jorgen Walker Peterson, Akihiro Isozaki, Kotaro Hiramatsu, Keisuke Goda
Summary: Cell sorting is an important tool in biological research and medicine. Raman-activated cell sorting (RACS) has gained attention for its ability to discriminate cells based on their intracellular chemical content without the need for labeling. However, the widespread use of RACS has been limited by a trade-off between throughput and measurement bandwidth. In this study, this trade-off is overcome and broadband RACS in the fingerprint region with a record high throughput is demonstrated. Real-time label-free sorting of microalgal cells based on their accumulation of carotenoids and polysaccharide granules is also showcased.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Dermatology
Aleksei Orlov, Amit Gefen
Summary: This study evaluated the performance of two types of single-use negative-pressure wound therapy systems and found that continuous and consistent negative-pressure delivery through controlled fluid management technology can improve the quality of surgical wound healing.
INTERNATIONAL WOUND JOURNAL
(2022)
Article
Dermatology
Maayan Lustig, Amit Gefen
Summary: This study developed a computer modeling framework to quantify how optimal envelopment disperses tissue stress concentrations near the sacrum. Results showed that using a minimum tissue deformation mattress can reduce strains and stresses in subdermal soft tissues, leading to longer safe times for supine support.
INTERNATIONAL WOUND JOURNAL
(2022)
Review
Dermatology
Mai Dabas, Dafna Schwartz, Dimitri Beeckman, Amit Gefen
Summary: This study provides an in-depth overview of the methodologies of artificial intelligence (AI) and their potential implementation in wound care and management. The findings highlight the positive impact and prospects of AI in improving healthcare quality and efficiency, as well as the emergence of AI-based remote consultation systems.
ADVANCES IN WOUND CARE
(2023)
Article
Dermatology
Aleksei Orlov, Amit Gefen
Summary: There are two design concepts for single-use negative pressure wound therapy systems: canister-based and canisterless. The canister-based technology provides stable delivery of negative pressure, while canisterless systems may result in loss of negative pressure. Research shows that continuous delivery of the intended pressure is necessary for an effective influence zone.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Dermatology
Aleksei Orlov, Amit Gefen
Summary: Prolonged use of positive airway pressure masks can lead to facial tissue breakdown, but this risk can be reduced with suitable cushioning materials. By comparing different dressings through experiments and computational modeling, it was found that foam-based dressings generally outperformed hydrocolloid dressings.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Dermatology
Aleksei Orlov, Amit Gefen
Summary: In this study, we developed a robotic system with two venous leg ulcers to test the fluid handling performance of three wound dressing types. The results indicated that foam-based dressing technology is not effective for managing exuding venous leg ulcers. Additionally, wound dressings containing superabsorbent polymers do not demonstrate equal performance in fluid handling for different wound scenarios.
INTERNATIONAL WOUND JOURNAL
(2023)
Review
Dermatology
Breda Cullen, Amit Gefen
Summary: Chronic wounds are a global issue that not only affects patients' health but also imposes a significant burden on society. This review explores the reasons for wound healing failure, the pathophysiology of chronic wounds, and the design and evaluation of dressings. It emphasizes the importance of understanding wound exudates and developing laboratory models for testing dressings. The findings have significant implications for improving dressing effectiveness, enhancing patient outcomes, and reducing the economic burden of chronic wounds.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Dermatology
Amit Gefen, Paulo Alves, Dimitri Beeckman, Jose Luis Lazaro-Martinez, Hadar Lev-Tov, Bijan Najafi, Terry Swanson, Kevin Woo
Summary: The mechanical performance of foam materials in foam-based wound dressings plays a crucial role in the clinical outcomes and cost of care. This article provides a comprehensive overview of the theoretical and practical considerations related to the mechanical performance of foams in wound dressings, based on published research and clinical experience. This information is important for clinicians, researchers, regulators, and buyers in the wound care field.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Neurosciences
Hadar Shaulian, Amit Gefen, Hen Biton, Alon Wolf
Summary: This study investigates a novel graded-stiffness offloading method for diabetic heel ulcers, which can effectively redistribute heel pressure and reduce focal stress concentration areas. The results show that the graded-stiffness offloading solution is more effective in reducing and redistributing peak pressure and pressure dose compared to existing solutions.
Article
Dermatology
Tomer Katz, Amit Gefen
Summary: Support surfaces play a crucial role in preventing pressure ulcers/injuries for at-risk patients. Hybrid support surfaces, combining reactive and active properties, provide pressure redistribution through the use of high-quality foam material inside inflatable air cells. This study uses computational modelling and simulations to investigate the effects of static and dynamic modes of a hybrid support surface on soft tissue loading at the buttocks. Results show that the dynamic mode effectively relieves deep tissue pressure under the sacral bone and generates a deep tissue offloading effect by shifting the loading towards the coccyx.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Dermatology
Charlotte Raepsaet, Paulo Alves, Breda Cullen, Amit Gefen, Jose Luis Lazaro-Martinez, Hadar Lev-Tov, Bijan Najafi, Nick Santamaria, Andrew Sharpe, Terry Swanson, Kevin Woo, Dimitri Beeckman
Summary: The aim of this project was to develop a core outcome set (COS) for clinical effectiveness studies of bordered foam dressings in the treatment of complex wounds. The project followed the COMET initiative and consisted of two phases. The outcomes were generated through a systematic review, qualitative study, Delphi consensus study, and consensus meeting.
JOURNAL OF TISSUE VIABILITY
(2023)
Article
Biology
Maayan Lustig, Yoram Epstein, Amit Gefen
Summary: This study developed a novel finite element modeling framework to evaluate and rate the biomechanical efficacy of ballistic protective plates (BPPs) in protecting the torso from non-penetrating impacts acquired on the battlefield.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Dermatology
Aleksei Orlov, Marino Ciliberti, Rosa Somma, Amit Gefen
Summary: In this study, we compared the fluid handling performance of different wound care products using advanced robotic technology. The single-use negative pressure wound therapy system emerged as the leading treatment option, while the foam dressing exhibited the poorest fluid handling performance.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Dermatology
Mai Dabas, Ida Kreychman, Tomer Katz, Amit Gefen
Summary: We investigated the reactions of healthy sacral skin to sustained mechanical loading by measuring inflammatory and thermal responses. Infrared thermography outperformed digital photography in detecting skin irritation trends. Polymeric membrane dressings were more effective in reducing inflammation and managing microclimate compared to polyurethane foam. Infrared thermography is a feasible method for monitoring skin health and risk for pressure ulcers.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Dermatology
Suzanne Kapp, Marie Gerdtz, Charne Miller, Amit Gefen, William Padula, Lauren Wilson, Michael Woodward, Nick Santamaria
Summary: Pressure injuries are common in aged care facilities and have a significant impact on healthcare systems. Remote expert wound nurse consultation shows potential in improving pressure injury outcomes, but more research is needed to determine its effectiveness and cost-effectiveness in residential aged care. This study describes a pilot randomized controlled trial investigating the intervention, with outcomes including wound healing rate, infection, quality of life, and cost of treatment. The results will inform future trials evaluating the clinical and economic benefits of remote expert wound nurse consultation in this setting.
INTERNATIONAL WOUND JOURNAL
(2023)