Article
Chemistry, Multidisciplinary
Jiahui He, Zhiya Fan, Yinping Tian, Weiwei Yang, Yichao Zhou, Qiang Zhu, Wanjun Zhang, Weijie Qin, Wen Yi
Summary: Light control of OGT activity in cells has been achieved by replacing a catalytically essential lysine residue with a genetically encoded photocaged lysine, providing a valuable chemical tool to control cellular O-GlcNAc with spatiotemporal precision and aiding in a better understanding of O-GlcNAc function.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Haihong Bai, Baoying Zhang, Xiaoqiang Cheng, Ju Liu, Xinghe Wang, Weijie Qin, Man Zhang
Summary: In this study, a new hydrophilic material GO-pDMAPS was prepared and successfully applied for N-glycopeptide enrichment from human urine, identifying a total of 1426 N-glycosylated sites corresponding to 766 N-glycoproteins from healthy subjects and patients with lung adenocarcinoma. This new material shows great potential for large-scale and in-depth N-glycoproteome research.
Article
Multidisciplinary Sciences
Liming Wu, Yaxian Cheng, Didi Geng, Zhiya Fan, Bingyi Lin, Qiang Zhu, Jingchao Li, Weijie Qin, Wen Yi
Summary: The O-GlcNAc modification plays a crucial role in regulating the intracellular trafficking and degradation of EGFR. It inhibits the interaction between HGS and STAM, impairing the formation of ESCRT-0 and leading to the accumulation and prolonged signaling of EGFR in cells. Additionally, glycosylation of HGS promotes tumor growth and chemoresistance in liver carcinoma cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Analytical
Bianbian Huo, Yuanyuan Liu, Lingjun Li, Weijie Qin
Summary: The study developed a novel chemical enrichment method (AFT-OCAP) for simultaneous analysis of O-GlcNAc chromatin-associated proteins (OCAPs) and mapping their binding DNA, providing valuable tools for understanding the functions of OCAPs and the regulatory machinery of O-GlcNAcylation on gene transcription.
Article
Physics, Atomic, Molecular & Chemical
Wenhui Ma, Hang Li, Yujie Wang, Qi Liu, Weijie Qin, Jiayu Liu, Huan Cheng, Wanjun Zhang
Summary: Extracellular vesicles (EVs) released from tumor cells and tissues are involved in intercellular communication and regulation of biological processes. The study introduces a TiO2-mediated multiomics EV preparation method (TiMMEP) for isolating and profiling proteins and metabolites from a small volume of plasma. By capturing EVs using TiO2 and releasing proteins and metabolites with acetonitrile, the method enables high-throughput analysis of EVs, potentially improving disease diagnosis and biomarker discovery.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Wei Fang, Zhuokun Du, Linlin Kong, Bin Fu, Guibin Wang, Yangjun Zhang, Weijie Qin
Summary: Single-cell analysis is an important method for studying the cellular heterogeneity in biological systems. However, the measurement of the proteome in single cells lags behind transcriptomics due to the lack of sensitive and high-throughput mass spectrometry methods. In this study, a strategy called SCP-MS1 was developed, which combines fast liquid chromatography separation, deep learning-based retention time prediction, and MS1-only acquisition for rapid and sensitive single-cell proteome analysis.
ANALYTICA CHIMICA ACTA
(2023)
Article
Nanoscience & Nanotechnology
Yue-yang Yu, Mei Yuan, Wei-jie Qin, Hai-hong Bai, Hong-zhuo Liu, Jin-jing Che
Summary: This study developed a novel strategy for separating liposomal-encapsulated drugs from plasma using TiO2 microspheres. The method was validated and proved to be suitable for quantitation of liposomal-encapsulated drugs in plasma samples.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Biochemical Research Methods
Baoying Zhang, Hang Li, Linlin Kong, Ningli Yang, Shengjie Yang, Lu Qi, Tong Liu, Xinghe Wang, Weijie Qin
Summary: Exosomal RNA from serum has potential as a biomarker for early cancer diagnosis and prognosis, but isolating high-quality exosomes and exoRNA remains challenging. In this study, a novel tandem enrichment method using titanium dioxide was developed to isolate exoRNA from serum. This method provides a simple, economical, rapid, and safe approach for exoRNA extraction from biological samples.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Yongle Chen, Hongxian Zhao, Tong Liu, Yangjun Zhang, Wanjun Zhang, Weijie Qin
Summary: Single and rare cell analysis provides unique insights into biological processes and diseases. However, current techniques for proteome analysis in trace amounts or single cells lag behind DNA and RNA techniques. We propose a MS/MS spectra merging strategy for improved low-input and single-cell proteome data analysis, which showed improved protein identification and potential for more sensitive studies.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Ningli Yang, Chuanping Zhao, Linlin Kong, Baoying Zhang, Chunguang Han, Yangjun Zhang, Xiaohong Qian, Weijie Qin
Summary: Extracellular vesicles (sEVs) play important roles in physiological and pathological processes, and their cellular uptake is crucial for regulating recipient cells. A novel strategy using lanthanide element labeling and ICP-MS was developed for the quantification of sEVs. This method demonstrated high labeling efficiency and stability, and revealed the internalization of sEVs by HeLa cells. The strategy can be used as a potential tool for studying sEVs in intracellular communication and cargo transportation.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Linlin Kong, Fengzhi Li, Wei Fang, Zhuokun Du, Guibin Wang, Yangjun Zhang, Woo-ping Ge, Wanjun Zhang, Weijie Qin
Summary: Single-cell omics is crucial for understanding population heterogeneity, identifying unique features of individual cells, and discovering minority subpopulations of interest. Protein N-glycosylation is a major post-translational modification that plays important roles in various biological processes. However, comprehensive N-glycoproteome profiling for single cells has been challenging due to limited sample amount and incompatibility with available enrichment strategies.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wei Fang, Zhuokun Du, Linlin Kong, Guibin Wang, Yangjun Zhang, Weijie Qin
Summary: Achieving deep protein coverage in proteome profiling of low-input and single-cell samples is challenging. A three-stage search strategy combining database reduction and Delta retention time (Delta RT) filtering improved peptide/protein identification and reproducibility. This strategy resulted in over 30% improvement in proteome coverage for single-cells and similar size samples, with enhanced reproducibility and higher differential protein discovery.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Hongxian Zhao, Yongle Chen, Hang Li, Yangjun Zhang, Wanjun Zhang, Weijie Qin
Summary: Profiling proteins is crucial for understanding biological systems, and a new angled-shape tip-based strategy has been developed for rapid sample preparation and sensitive proteomic profiling. The angled-shape tip provides a reactor for the entire proteomic sample processing workflow, eliminating sample transfer-induced protein loss. This approach shows great potential for in-depth proteomic profiling of rare cells (<1000 cells) in both biological research and clinical applications.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Yuanyuan Li, Chaoshuang Xia, Hongxian Zhao, Yuping Xie, Yangjun Zhang, Wanjun Zhang, Yongliang Yu, Jianhua Wang, Weijie Qin
Summary: The cell surface membrane proteome, a class of proteins that mediate communication between cells and their environment, is challenging to purify and study. This study developed a new photolabeling probe for efficient tagging and enrichment of cell surface membrane proteins, enabling a deeper understanding of their structure and function.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Weidi Xiao, Shuai Huang, Yuan Gao, Tong Liu, Naikang Li, Xinying Sui, Yonghong Wang, Guan Yang, Lei Chang, Xianjiang Kang, Changzhu Duan, Weijie Qin, Ping Xu, Yanchang Li
Summary: The study introduces a new method TUF-WB+ that significantly improves the robustness and sensitivity of polyubiquitination signal detection, while also reducing the experimental operation time. The ThUBD-HRP probe is capable of conducting in situ polyubiquitination detection in cells, providing a powerful tool for ubiquitination signal detection.