Maximizing Cumulative Trypsin Activity with Calcium at Elevated Temperature for Enhanced Bottom-Up Proteome Analysis

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

(2022) Jessica L. Nickerson et al.   Jove-Journal of Visualized Experiments

The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences

(2021) Yasset Perez-Riverol et al.   NUCLEIC ACIDS RESEARCH

Immobilized-enzyme reactors integrated into analytical platforms: Recent advances and challenges

(2021) B. Wouters et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Evaluation and minimization of nonspecific tryptic cleavages in proteomic sample preparation

(2020) Zhilong Lin et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry

(2020) Xiaoqin Zhong et al.   Nature Communications

BAC-DROP: Rapid Digestion of Proteome Fractionated via Dissolvable Polyacrylamide Gel Electrophoresis and Its Application to Bottom-Up Proteomics Workflow

(2020) Ayako Takemori et al.   JOURNAL OF PROTEOME RESEARCH

Cysteine alkylation methods in shotgun proteomics and their possible effects on methionine residues

(2020) Ksenia G. Kuznetsova et al.   Journal of Proteomics

Temperature-induced aggregation behavior in bovine pancreas trypsin solutions

(2019) Sofia Trampari et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Enhanced trypsin on a budget: Stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications

(2019) Søren Heissel et al.   PLoS One

The benefits (and misfortunes) of SDS in top-down proteomics

(2018) Carolyn Kachuk et al.   Journal of Proteomics

Immobilized enzyme-based analytical tools in the -omics era: Recent advances

(2018) Marina Naldi et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Label-free protein quantification after ultrafast digestion of complex proteomes using ultrasonic energy and immobilized-trypsin magnetic nanoparticles

(2018) Gonçalo Martins et al.   TALANTA

The Impact of Commonly Used Alkylating Agents on Artifactual Peptide Modification

(2017) Peter G. Hains et al.   JOURNAL OF PROTEOME RESEARCH

imFASP: An integrated approach combining in-situ filter-aided sample pretreatment with microwave-assisted protein digestion for fast and efficient proteome sample preparation

(2016) Qun Zhao et al.   ANALYTICA CHIMICA ACTA

On the Temperature Dependence of Enzyme-Catalyzed Rates

(2016) Vickery L. Arcus et al.   BIOCHEMISTRY

Controlling nonspecific trypsin cleavages in LC-MS/MS-based shotgun proteomics using optimized experimental conditions

(2015) Pan Fang et al.   ANALYST

Insight into Trypsin Miscleavage: Comparison of Kinetic Constants of Problematic Peptide Sequences

(2015) Tereza Šlechtová et al.   ANALYTICAL CHEMISTRY

Surfactant-Aided Precipitation/on-Pellet-Digestion (SOD) Procedure Provides Robust and Rapid Sample Preparation for Reproducible, Accurate and Sensitive LC/MS Quantification of Therapeutic Protein in Plasma and Tissues

(2015) Bo An et al.   ANALYTICAL CHEMISTRY

Quantitative proteomics reveals the kinetics of trypsin-catalyzed protein digestion

(2014) Yanbo Pan et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Critical assessment of the spectroscopic activity assay for monitoring trypsin activity in organic–aqueous solvent

(2013) Andrew M.J. Crowell et al.   ANALYTICAL BIOCHEMISTRY

Getting intimate with trypsin, the leading protease in proteomics

(2013) Elien Vandermarliere et al.   MASS SPECTROMETRY REVIEWS

Using Guanidine-Hydrochloride for Fast and Efficient Protein Digestion and Single-step Affinity-purification Mass Spectrometry

(2012) Jon W. Poulsen et al.   JOURNAL OF PROTEOME RESEARCH

Implications of partial tryptic digestion in organic–aqueous solvent systems for bottom-up proteome analysis

(2011) Mark J. Wall et al.   ANALYTICA CHIMICA ACTA

Rapid Characterization of Protein Chips Using Microwave-Assisted Protein Tryptic Digestion and MALDI Mass Spectrometry

(2011) Na Young Ha et al.   LANGMUIR

A Quantitative Study of the Effects of Chaotropic Agents, Surfactants, and Solvents on the Digestion Efficiency of Human Plasma Proteins by Trypsin

(2010) Jennifer L. Proc et al.   JOURNAL OF PROTEOME RESEARCH

High-Throughput Middle-Down Analysis Using an Orbitrap

(2010) Joe Cannon et al.   JOURNAL OF PROTEOME RESEARCH

Rapid and efficient protein digestion using trypsin-coated magnetic nanoparticles under pressure cycles

(2010) Byoungsoo Lee et al.   PROTEOMICS

An improved trypsin digestion method minimizes digestion-induced modifications on proteins

(2009) Da Ren et al.   ANALYTICAL BIOCHEMISTRY

The molecular basis of the effect of temperature on enzyme activity

(2009) Roy M. Daniel et al.   BIOCHEMICAL JOURNAL

Digestion completeness of microwave-assisted and conventional trypsin-catalyzed reactions

(2009) P. Muralidhar Reddy et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Sodium-deoxycholate-assisted tryptic digestion and identification of proteolytically resistant proteins

(2008) Yong Lin et al.   ANALYTICAL BIOCHEMISTRY