Article
Materials Science, Multidisciplinary
Olga S. Ovchinnikova, Nikolay Borodinov, Artem A. Trofimov, Steven T. King, Matthias Lorenz, William Lamberti, David Abmayr, Anton Ievlev
Summary: Chemical imaging of polymers and polymer blends has traditionally been done using ToF-SIMS with limited spatial resolution, but recent work has focused on using HIM-SIMS for higher resolution. By utilizing HIM-SIMS, differentiation between PE and PP as well as imaging of phase-separated domains within PE has been achieved, demonstrating its potential for analyzing complex polymeric systems.
ACS APPLIED POLYMER MATERIALS
(2021)
Review
Polymer Science
Hao Mei, Travis S. Laws, Tanguy Terlier, Rafael Verduzco, Gila E. Stein
Summary: ToF-SIMS is a powerful tool for chemical analysis of surfaces, particularly in polymer science, due to its ability to detect a broad mass range with good resolution. It can distinguish between polymers with similar compositions or functional groups without the need for chemical labeling techniques. ToF-SIMS can generate both two-dimensional images and three-dimensional depth profiles, providing valuable insights for characterizing polymer surfaces.
JOURNAL OF POLYMER SCIENCE
(2022)
Article
Chemistry, Analytical
Michael S. Westphall, Kenneth W. Lee, Colin Hemme, Austin Z. Salome, Keaton Mertz, Timothy Grant, Joshua J. Coon
Summary: This article describes an apparatus that can cryogenically land particles from the ion beam of a mass spectrometer onto transmission electron microscope grids for cryo-electron microscopy. This system also allows for the controlled formation of thin films of amorphous ice on the grid surface. The study demonstrates that using this cryogenic landing device significantly improves the structural preservation of deposited protein-protein complexes compared to room temperature landings, and landing under cryogenic conditions can increase the diversity of particle orientations, enabling improved 3D structural interpretation.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Matthias Lorenz, Junting Zhang, Alexander G. Shard, Jean-Luc Vorng, Paulina D. Rakowska, Ian S. Gilmore
Summary: A technique for directed transfer of molecules onto a sample surface inside a ToF-SIMS instrument using GCIB sputtering is introduced, with an example application in ME SIMS. This method does not require solvents, can be interlaced with SIMS depth profiling and 3D imaging cycles, and allows fine tuning of molecular deposition. Further study and optimization are needed to enhance signal ratios for drug molecules.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Florent Penen, Rene Raave, Annemarie Kip, Sandra Heskamp, Per Malmberg
Summary: ToF-SIMS imaging is a quick and sensitive technique that can visualize HER2 specific radiotracers in ovarian and breast cancer xenograft tumor sections to reflect the heterogeneous distribution of trastuzumab.
MICROCHEMICAL JOURNAL
(2022)
Article
Microbiology
Ekaterina R. Kotlova, Svetlana V. Senik, Bairta S. Manzhieva, Anna A. Kiyashko, Natalia V. Shakhova, Roman K. Puzansky, Sergei V. Volobuev, Alexander D. Misharev, Eugeny B. Serebryakov, Nadezhda V. Psurtseva
Summary: Phosphatidylcholines (PC) are the main membrane lipid constituents, synthesized and remodeled in basidiomycetes. The molecular profile of PC is determined by genetic features and environment, with a diverse range of molecular species. Hierarchical cluster and principal component analyses revealed four main types of PC profiles, independent of taxonomic position and ecology.
Article
Biochemistry & Molecular Biology
Michal Wronski, Jakub Trawinski, Lukasz Komsta, Robert Skibinski
Summary: Chemometric analysis identified nine forced degradation products of maraviroc. This study subjected the antiretroviral drug to various stress conditions such as photolysis, oxidation, hydrolysis, and electrochemical transformation. It was found that maraviroc is susceptible to UVA, H2O2, and electrochemical degradation, while being resistant to hydrolysis. Electrochemical transformation, especially on the platinum electrode, partially mimics the forced degradation processes, particularly in terms of redox reactions. These findings suggest that electrochemical methods can be cost-effective and rapid supplements to commonly used forced degradation procedures.
Article
Biochemical Research Methods
Robert P. Pellegrinelli, Lei Yue, Eduardo Carrascosa, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal, Thomas R. Rizzo
Summary: This article presents a systematic method of identifying reducing-end anomers of glycans using ultrahigh-resolution ion mobility, collision-induced dissociation, and cryogenic infrared spectroscopy. The results confirm that the infrared spectra can rapidly identify precursor glycans with a single anomer.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Agriculture, Multidisciplinary
Clara Pedrazzani, Francesca Vanara, Dhaka Ram Bhandari, Renato Bruni, Bernhard Spengler, Massimo Blandino, Laura Righetti
Summary: 5-n-Alkylresorcinols (AR) are bioactive compounds found in cereals, with varying content influenced by environmental and agronomic factors. The spatial distribution of AR in wheat kernels was mainly localized in the testa and outer pericarp.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Analytical
Hernando Rosales-Solano, Victor Galievsky, Khaled Murtada, Pavle Radovanovic, Janusz Pawliszyn
Summary: In this study, a novel on-site approach for detecting and differentiating enriched unsaturated lipids was proposed by coupling SPME probes with Raman spectroscopy. Results showed that the C18/PAN coating exhibited less interference when analyzing unsaturated lipids, and Raman spectroscopy was found to be particularly useful for analyzing lipid unsaturation. The developed protocol demonstrated great SPME activity and high detection ability for various unsaturated lipids in different complex matrixes.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Evgeny A. Idelevich, Betty Nedow, Marcus Vollmer, Karsten Becker
Summary: Over the past decade, MALDI-TOF mass spectrometry (MS) has become a standard method for identifying bacteria and yeasts. However, further optimization of the identification process is necessary to streamline workflows and save resources. This study evaluated the use of a multipurpose benchtop tool, MBT FAST Shuttle IVD, for accelerated drying of liquid assay components on a MALDI target. The results showed that using the MBT FAST Shuttle significantly reduced drying time and improved identification success.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Chemistry, Physical
Yajun Deng, Qianhong Wu, Zhenchao Li, Xin Huang, Shihang Rao, Yunfeng Liang, Hailong Lu
Summary: The wettability of quartz crystal faces is determined by the concentration of hydroxyl groups on the surfaces, with molecular dynamics simulations confirming experimental results. Surface hydroxyl groups were found to control the wettability of quartz crystal faces.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Environmental Sciences
Alexandra L. Klodt, Marley Adamek, Monica Dibley, Sergey A. Nizkorodov, Rachel E. O'Brien
Summary: Secondary organic aerosol (SOA) generated from the photooxidation of aromatic compounds in the presence of oxides of nitrogen (NOx) efficiently absorbs UV and visible radiation. The photodegradation of chromophoric compounds in SOA causes gradual photobleaching, which is influenced by the surrounding matrix.
ATMOSPHERIC CHEMISTRY AND PHYSICS
(2022)
Article
Chemistry, Analytical
Jiaxin Feng, Hansen Zhao, Xiaoyun Gong, Meng-Chan Xia, Lesi Cai, Huan Yao, Xu Zhao, Zihe Yan, Zhanping Li, Honggang Nie, Xiaoxiao Ma, Sichun Zhang
Summary: Research has shown that small MPs are highly toxic and current methods for detection and analysis are inadequate. A new method using SIMS imaging allows for rapid and accurate identification and spatial mapping of small MPs in organisms, offering a new approach to study their biological effects.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Paola Aguei-Gonzalez, Bao Guobin, Maria A. Gomes de Castro, Silvio O. Rizzoli, Nhu T. N. Phan
Summary: This study used secondary ion mass spectrometry imaging to investigate the lipid organization in the plasma membranes of hippocampal neurons. Distinct lipid organization and significant alterations in levels of membrane lipids, especially ceramides, phosphatidylserines, phosphatidic acids, and triacylglycerols, were observed under the treatments of tetrodotoxin (TTX) and bicuculline (BIC). Many types of membrane lipids are suggested to be affected by, and involved in, the regulation of neuronal function.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Physics, Atomic, Molecular & Chemical
Sanna Samfors, Marcus Stahlman, Martina Klevstig, Jan Boren, John S. Fletcher
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2019)
Article
Biochemistry & Molecular Biology
Mai H. Philipsen, Nhu T. N. Phan, John S. Fletcher, Per Malmberg, Andrew G. Ewing
ACS CHEMICAL NEUROSCIENCE
(2018)
Article
Biophysics
Marwa Munem, Oscar Zaar, Kelly Dimovska Nilsson, Noora Neittaanmaki, John Paoli, John S. Fletcher
Review
Radiology, Nuclear Medicine & Medical Imaging
Tiffany Porta Siegel, Gregory Hamm, Josephine Bunch, Jo Cappell, John S. Fletcher, Kristina Schwamborn
MOLECULAR IMAGING AND BIOLOGY
(2018)
Article
Chemistry, Multidisciplinary
Hua Tian, Louis J. Sparvero, Paul Blenkinsopp, Andrew A. Amoscato, Simon C. Watkins, Hulya Bayir, Valerian E. Kagan, Nicholas Winograd
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Chemistry, Analytical
Kelly Dimovska Nilsson, Martin Palm, James Hood, Jake Sheriff, Anne Farewell, John S. Fletcher
ANALYTICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Ibrahim Kaya, Eva Jennische, Johan Dunevall, Stefan Lange, Andrew G. Ewing, Per Malmberg, Ahmet Tarik Baykal, John S. Fletcher
ACS CHEMICAL NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Mai Hoang Philipsen, Nhu T. N. Phan, John Stephen Fletcher, Andrew G. Ewing
ACS CHEMICAL NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Ibrahim Kaya, Eva Jennische, Stefan Lange, Ahmet Tarik Baykal, Per Malmberg, John S. Fletcher
JOURNAL OF NEUROCHEMISTRY
(2020)
Article
Chemistry, Analytical
Sanna Samfors, John S. Fletcher
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 13
(2020)
Article
Multidisciplinary Sciences
Vidhi Pareek, Hua Tian, Nicholas Winograd, Stephen J. Benkovic
Article
Biophysics
Kelly Dimovska Nilsson, Noora Neittaanmaki, Oscar Zaar, Tina B. Angerer, John Paoli, John S. Fletcher
Article
Biochemical Research Methods
Ibrahim Kaya, Sanna Samfors, Malin Levin, Jan Boren, John S. Fletcher
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2020)
Article
Chemistry, Physical
Kelly Dimovska Nilsson, Johan Granden, Anne Farewell, John S. Fletcher
Summary: ToF-SIMS was used to analyze the chemical changes in the bacterial envelope of a fabF knock-out Escherichia coli strain. The results showed a substantial depletion of FA(18:1) in the fabF mutant during logarithmic growth, as well as a reduction in FA(cp19:0) during stationary phase. Additionally, the wild type strain exhibited a reduction in cyclopropanation at lower culture temperatures and a 'thickening' of the lipid A layer on the surface of the bacteria during stationary phase.
SURFACE AND INTERFACE ANALYSIS
(2021)
Article
Chemistry, Multidisciplinary
Inci Barut, Xiulan He, Erol Sener, Sanna Samfors, Andrew G. Ewing, John S. Fletcher
Summary: The abnormal functioning of proteasome is associated with crucial pathological conditions, such as neurodegeneration. However, the complex underlying causes at the cellular level are still unclear, and there are conflicting reports on the neuroprotective and neurodegenerative effects of proteasomal inhibitors like lactacystin, which are used as models for neurodegenerative diseases. The conflicting results may be due to different doses of lactacystin. Hence, a dose-dependent study was conducted to investigate the effects of lactacystin on cell membrane lipid profile and exocytosis dynamics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)