Review
Physiology
Pura Bolanos, Juan C. Calderon
Summary: The excitation-contraction coupling in skeletal muscle is a crucial link between membrane excitation and mechanical contraction mediated by Ca2+, involving multiple mechanisms of Ca2+ release and recycling.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Chemistry, Analytical
Teliang Zhang, Xueliang Wang, Youjun Zeng, Songfeng Huang, Xiaoqi Dai, Weifu Kong, Qian Liu, Jiajie Chen, Junle Qu, Yonghong Shao
Summary: Surface plasmon resonance microscopy (SPRM) is widely used in biological fields for its high spatial resolution and label-free detection. In this study, a home-built SPRM system based on total internal reflection (TIR) was utilized to study the imaging of a single nanoparticle. By using a ring filter and deconvolution algorithm, the parabolic tail of the nanoparticle image was removed, achieving a spatial resolution of 248 nm. Additionally, specific binding between human IgG antigen and goat anti-human IgG antibody was successfully measured using TIR-based SPRM, demonstrating its capability in imaging sparse nanoparticles and monitoring biomolecular interactions.
Article
Chemistry, Analytical
Jiying Xu, Caixin Huang, Liangju Li, Ying Zhao, Zhenpeng Guo, Yi Chen, Pengfei Zhang
Summary: ESM technology allows for the measurement of ligand interactions with membrane proteins and analysis of cell adhesion properties and cell activity changes, providing a new method for studying cellular processes and drug screening.
Article
Biochemistry & Molecular Biology
Joerg Nikolaus, Kasey Hancock, Maria Tsemperouli, David Baddeley, Erdem Karatekin
Summary: Researchers used a polarization-controlled total internal reflection fluorescence (pTIRF) microscope to monitor the fusion of proteoliposomes with planar lipid bilayers, investigating membrane fusion pore dynamics. By varying excitation polarization angle, they identified a dye-dependent optimal polarization for maximal fluorescence increase upon fusion, aiding event detection and lipid transfer kinetics analysis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Polymer Science
J. Alejandro Rivera-Moran, Peter R. Lang
Summary: This paper investigates the influence of noise effects, particle size, and sampling time on total internal reflection microscopy (TIRM) measurement results. It finds that detector shot noise and prolonged sampling times may cause erroneous results in steep parts of the interaction potential, while background noise has negligible effects. Noise does not significantly affect dynamic data, but lengthy sampling times and small probe particle radii can cause issues. Additionally, the study observes that dynamic results may differ from standard hydrodynamic predictions due to partial slip.
Article
Cell Biology
Julien Louradour, Olivier Bortolotti, Eleonora Torre, Isabelle Bidaud, Ned Lamb, Anne Fernandez, Jean-Yves Le Guennec, Matteo E. Mangoni, Pietro Mesirca
Summary: This study reveals a novel role of Ca(v)1.3 channels in initiating and maintaining automaticity in dormant SANC upon beta-adrenergic stimulation.
Article
Pharmacology & Pharmacy
Paulo A. De Oliveira, Estefania Moreno, Nil Casajuana-Martin, Veronica Casado-Anguera, Ning-Sheng Cai, Gisela Andrea Camacho-Hernandez, Hu Zhu, Alessandro Bonifazi, Matthew D. Hall, David Weinshenker, Amy Hauck Newman, Diomedes E. Logothetis, Vicent Casado, Leigh D. Plant, Leonardo Pardo, Sergi Ferre
Summary: Recent studies suggest that the heteromers of mu-opioid receptors (MORs) and galanin Gal(1) receptors (Gal(1)Rs) in the mesencephalon play a role in mediating the dopaminergic effects of opioids. This study provides evidence for a predominant homodimeric structure of MOR and Gal(1)R when expressed individually and for their preference to form functional heterotetramers when co-expressed. The results show that this heteromerization leads to a change in G-protein coupling, providing a framework for Gs-Gi antagonist interaction.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Alexander Maltsev, Edward Evdokimovskii, Yury M. Kokoz
Summary: Protein kinase C is a superfamily of intracellular effector molecules that play crucial roles in controlling cellular functions. This study identified a wide expression of PKC transcripts in left ventricular myocytes, with PKC activity tonically regulating various cellular processes, including voltage-gated Ca2+ currents and nitric oxide production. The findings suggest that PKC-mediated Ca2+ signaling may play a significant role in the development of cardiovascular pathologies and myocardial plasticity at the cardiomyocyte level.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Josette Zaklit, Alex Cabrera, Aaron Shaw, Rita Aoun, P. Thomas Vernier, Normand Leblanc, Gale L. Craviso
Summary: The study found that under certain conditions, ultra-short electric pulses can stimulate the release of catecholamines through exocytosis, with real-time observations showing that the release is Ca2+-dependent and the release events are relatively prolonged. Furthermore, the effectiveness of this stimulation method was demonstrated, indicating its potential as a novel electrostimulation modality for neurosecretion.
BIOELECTROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Dariia O. Dryn, Mariia I. Melnyk, Donal Melanaphy, Igor V. Kizub, Christopher D. Johnson, Alexander V. Zholos
Summary: This study demonstrates for the first time the biphasic contractile reaction of TRPV4 in rat pulmonary artery, showing relaxation followed by a contraction. The relaxation phase is induced by BKCa activation, while the subsequent contraction is mediated by TRPV4-dependent depolarization and Ca-L activation. Similar changes in membrane potential are observed with the activation of TRPM8 in the rat tail artery.
Article
Biochemistry & Molecular Biology
Maria Paschou, Danai Liaropoulou, Vasileia Kalaitzaki, Spiros Efthimiopoulos, Panagiota Papazafiri
Summary: In this study, the downregulation of APP in SH-SY5Y cells altered the expression of ion channels and signaling proteins involved in synaptic and Ca2+ signaling. The levels of GluR2 and GluR4 subunits of AMPAR were increased, as well as the expression of ER-residing proteins. Changes in phosphorylation states of AKT, PP2A, CaMKIV, GSK, ERK1/2, CaMKII, and CREB were observed, indicating an interconnection between Ca2+ signaling from the plasma membrane and ER to the nucleus. Interestingly, reduced responses to physiological stimuli, particularly in mobilizing Ca2+ from the ER, were detected in APP knockdown cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Kirin D. Gada, Jordie M. Kamuene, Takeharu Kawano, Leigh D. Plant
Summary: The cell surfaceome plays a crucial role in biology, but the identification and regulation of proteins on the cell membrane remains challenging. TIRFM is a precise and effective technique that helps to locate proteins on the cell membrane by limiting image depth and improving signal-to-noise ratio.
Article
Cardiac & Cardiovascular Systems
E. A. Rog-Zielinska, R. Moss, W. Kaltenbacher, J. Greiner, P. Verkade, G. Seemann, P. Kohl, M. B. Cannell
Summary: Detailed knowledge of the ultrastructure of intracellular compartments is essential for understanding cell function, particularly in cardiac muscle cells where the close apposition of transverse-tubule (TT) and sarcoplasmic reticulum (SR) membranes supports excitation-contraction coupling. Using ultra-rapid high-pressure freezing and electron microscopy, the study compared the fine structure of TT and SR in rabbit and mouse ventricular cardiomyocytes. The results suggest that HPF-preserved samples exhibit larger SR terminal cisternae and provide novel insights into the cardiac physiology of TT and SR ultrastructure.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2021)
Article
Cell Biology
Camille S. Wang, Lisa M. Monteggia, Ege T. Kavalali
Summary: Calcium (Ca2+) signaling is tightly regulated within a presynaptic bouton, involving evoked, spontaneous, and baseline signals derived from multiple sources. These signals have non-overlapping domains within the synapse and interact through synaptic vesicle turnover.
Article
Chemistry, Physical
Jing Zeng, Wen Fan, Kangyu Jia, Shuhua Tu, Limin Wu
Summary: A novel retroreflective structural color film (RSCF) based on interference and total internal reflection (TIR) was reported. The film consists of a microscale polymer hemisphere array, displaying different colors under coaxial and noncoaxial illumination and observation. Additionally, an elastic polyurethane-based RSCF can exhibit dynamically changing colors during uniaxial tensile/compressive deformation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Cardiac & Cardiovascular Systems
Lei Li, Jing Li, Benjamin M. Drum, Yi Chen, Haifeng Yin, Xiaoyun Guo, Stephen W. Luckey, Merle L. Gilbert, G. Stanley McKnight, John D. Scott, L. Fernando Santana, Qinghang Liu
CARDIOVASCULAR RESEARCH
(2017)
Article
Physiology
Sendoa Tajada, Claudia M. Moreno, Samantha O'Dwyer, Sean Woods, Daisuke Sato, Manuel F. Navedo, L. Fernando Santana
JOURNAL OF GENERAL PHYSIOLOGY
(2017)
Article
Clinical Neurology
Benoit J. Gentil, Erin O'Ferrall, Colin Chalk, Luis F. Santana, Heather D. Durham, Rami Massie
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
(2017)
Article
Biochemistry & Molecular Biology
Debapriya Ghosh, Madeline Nieves-Cintron, Sendoa Tajada, Ingrid Brust-Mascher, Mary C. Horne, Johannes W. Hell, Rose E. Dixon, Luis F. Santana, Manuel F. Navedo
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2018)
Article
Biochemical Research Methods
Daisuke Sato, Rose E. Dixon, Luis F. Santana, Manuel F. Navedo
PLOS COMPUTATIONAL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
F. Donelson Smith, Mitchell H. Omar, Patrick J. Nygren, Joseph Soughayer, Naoto Hoshi, Ho-Tak Lau, Calvin G. Snyder, Tess C. Branon, Debapriya Ghosh, Lorene K. Langeberg, Alice Y. Ting, Luis F. Santana, Shao-En Ong, Manuel F. Navedo, John D. Scott
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Cell & Tissue Engineering
Ana De La Mata, Sendoa Tajada, Samantha O'Dwyer, Collin Matsumoto, Rose E. Dixon, Nirmala Hariharan, Claudia M. Moreno, Luis Fernando Santana
Article
Biology
Maria Paz Prada, Arsalan U. Syed, Olivia R. Buonarati, Gopireddy R. Reddy, Matthew A. Nystoriak, Debapriya Ghosh, Sergi Simo, Daisuke Sato, Kent C. Sasse, Sean M. Ward, Luis F. Santana, Yang K. Xiang, Johannes W. Hell, Madeline Nieves-Cintron, Manuel F. Navedo
Article
Medicine, Research & Experimental
Arsalan U. Syed, Gopireddy R. Reddy, Debapriya Ghosh, Maria Paz Prada, Matthew A. Nystoriak, Stefano Morotti, Eleonora Grandi, Padmini Sirish, Nipavan Chiamvimonvat, Johannes W. Hell, Luis F. Santana, Yang K. Xiang, Madeline Nieves-Cintron, Manuel F. Navedo
JOURNAL OF CLINICAL INVESTIGATION
(2019)
Article
Physiology
Daisuke Sato, Gonzalo Hernandez-Hernandez, Collin Matsumoto, Sendoa Tajada, Claudia M. Moreno, Rose E. Dixon, Samantha O'Dwyer, Manuel F. Navedo, James S. Trimmer, Colleen E. Clancy, Marc D. Binder, L. Fernando Santana
JOURNAL OF GENERAL PHYSIOLOGY
(2019)
Article
Biology
Jie-Xian Dong, Yongam Lee, Michael Kirmiz, Stephanie Palacio, Camelia Dumitras, Claudia M. Moreno, Richard Sando, L. Fernando Santana, Thomas C. Sudhof, Belvin Gong, Karl D. Murray, James S. Trimmer
Article
Cell Biology
Scott A. Tiscione, Oscar Vivas, Kenneth S. Ginsburg, Donald M. Bers, Daniel S. Ory, Luis F. Santana, Rose E. Dixon, Eamonn J. Dickson
JOURNAL OF CELL BIOLOGY
(2019)
Article
Biology
Nicholas C. Vierra, Michael Kirmiz, Deborah van der List, L. Fernando Santana, James S. Trimmer
Article
Cell Biology
Benjamin M. Drum, Can Yuan, Ana de la Mata, Nathan Grainger, L. Fernando Santana
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2020)
Article
Multidisciplinary Sciences
Samantha C. O'Dwyer, Stephanie Palacio, Collin Matsumoto, Laura Guarina, Nicholas R. Klug, Sendoa Tajada, Barbara Rosati, David McKinnon, James S. Trimmer, L. Fernando Santana
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)