Article
Biochemistry & Molecular Biology
Ajanta Chatterji, Debasmita Banerjee, Timothy R. Billiar, Rajib Sengupta
Summary: This article discusses the role of S-nitrosylation in cellular processes, as well as the importance of thioredoxin and S-nitrosoglutathione reductase in combating nitric oxide pathology and modulating inflammation.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Multidisciplinary Sciences
Petra Kameritsch, Miriam Singer, Christoph Nuernbergk, Natalia Rios, Anibal M. Reyes, Kjestine Schmidt, Julian Kirsch, Holger Schneider, Susanna Mueller, Kristin Pogoda, Ruicen Cui, Thomas Kirchner, Cor de Wit, Baerbel Lange-Sperandio, Ulrich Pohl, Marcus Conrad, Rafael Radi, Heike Beck
Summary: The study reveals the crucial role of endothelial TrxR2 in regulating the steady-state concentration of peroxynitrite and maintaining vascular integrity. Deletion of the TrxR2 gene leads to increased vascular stiffness, hypertrophy, and renal abnormalities. Loss of TrxR2 results in elevated peroxynitrite levels in both vascular endothelial cells and vessels, indicating a potential disruption of vascular homeostasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Divya Seth, Colin T. Stomberski, Precious J. McLaughlin, Richard T. Premont, Kathleen Lundberg, Jonathan S. Stamler
Summary: The aim of this study was to investigate the interactions between nitric oxide synthase (NOS) isoforms and proteins, as well as the mechanisms by which they mediate S-nitrosylation. The results showed that each NOS isoform interacts with and S-nitrosylates many proteins, but a large proportion of S-nitrosylated proteins are not associated with NOS. Additionally, the interactomes and S-nitrosylomes of individual NOS isoforms are largely unique, indicating specific roles for each isoform in S-nitrosylation.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Esha Sircar, Detcho A. Stoyanovsky, Timothy R. Billiar, Arne Holmgren, Rajib Sengupta
Summary: Intracellular reduced glutathione (GSH) is the most abundant intracellular thiol with concentrations ranging from 1-10 mM, playing a crucial role as a potent cellular antioxidant and denitrosylating agent against redox stress. Our study demonstrates GSH mediated denitrosylation of protein nitrosothiols in HepG2 cells using a unique spin-trapping mechanism and identifies two previously unknown substrates of GSH mediated S-denitrosylation.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Benjamin Lau, Hossein Fazelinia, Ipsita Mohanty, Serena Raimo, Margarita Tenopoulou, Paschalis-Thomas Doulias, Harry Ischiropoulos
Summary: This study identified and analyzed S-nitrosocysteine residues in mouse heart proteins, with a significant enrichment of these modifications in mitochondrial proteins participating in metabolism. The major functional pathways impacted by protein S-nitrosylation include regulatory proteins in glycolysis, TCA cycle, oxidative phosphorylation, and ATP production. The meta-analysis of the proteomic data supports the hypothesis that nitric oxide signaling via protein S-nitrosylation is a regulator of cardiomyocyte metabolism to ensure optimal cardiac function.
Review
Plant Sciences
Anjali Pande, Bong Gyu Mun, Waqas Rahim, Murtaza Khan, Da Sol Lee, Geun Mo Lee, Tiba Nazar Ibrahim Al Azzawi, Adil Hussain, Chang Kil Kim, Byung Wook Yun
Summary: The interaction between nitric oxide (NO) and phytohormones is mainly influenced by NO-mediated post-translational modifications (PTMs), including direct and indirect nitrosylation. This interaction plays an important role in regulating physiological processes in plants and responding to environmental stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Surupa Chakraborty, Esha Sircar, Camelia Bhattacharyya, Ankita Choudhuri, Akansha Mishra, Sreejita Dutta, Sneha Bhatta, Kumar Sachin, Rajib Sengupta
Summary: S-nitrosylation of proteins, resulting from the reaction between cysteine thiols and nitric oxide (NO), is associated with diseases and protein malfunction. It has rapidly emerged as a major cause of various metabolic, respiratory, and cardiac disorders, particularly cancer and neurodegeneration. The field of redox biochemistry has explored other covalently modified versions of proteins, but the S-nitrosoproteome, which refers to the diverse and dynamic repertoire of S-nitrosylated proteins, remains relatively unexplored. Research in this area is uncovering the clinical importance of S-nitrosylation in disease etiology and providing new avenues for prompt diagnosis.
Review
Biochemistry & Molecular Biology
Hyang-Mi Lee, Ji Woong Choi, Min Sik Choi
Summary: Ischemia-reperfusion injury (IRI) is a damage process induced in hypoxic tissue when oxygen is supplied after ischemia. Restoration of reduced nitric oxide (NO) levels may alleviate reperfusion injury in ischemic organs, with the protective mechanism due to anti-inflammatory and antioxidant effects of NO, as well as regulation of cell signaling pathways. S-nitrosylation (SNO) mediates the detrimental or protective effect of NO depending on the nitrosylated target protein, which also plays a role in the IRI process.
Review
Biochemistry & Molecular Biology
Gizem Kayki-Mutlu, Walter J. Koch
Summary: Cardiac diseases, especially heart failure, are the leading cause of morbidity and mortality globally. The loss of beta-adrenoceptor-mediated inotropic reserve and the elevated activities of G protein-coupled receptor kinases and beta-arrestins after heart injury/stress play significant roles in heart failure. Nitric oxide produced by NO synthase enzymes modulates GPCR regulators profoundly through S-nitrosylation, independent of its canonical signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Doria Boulghobra, Mathilde Dubois, Beatrice Alpha-Bazin, Florence Coste, Maxime Olmos, Sandrine Gayrard, Isabelle Bornard, Gregory Meyer, Jean-Charles Gaillard, Jean Armengaud, Cyril Reboul
Summary: Exercise training increases the activation of eNOS and its localization in mitochondria, leading to enhanced nitric oxide production, which may represent a key mechanism of exercise-induced cardioprotection.
BASIC RESEARCH IN CARDIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Sonia Simao, Rafaela Ribeiro Agostinho, Antonio Martinez-Ruiz, Ines Maria Araujo
Summary: Ras proteins, as small GTPases, play important roles in cell proliferation, migration, differentiation and survival. They are often mutated in cancer and can be modified by S-nitrosylation, which affects their cellular dynamics and signaling pathways.
Article
Chemistry, Multidisciplinary
Rajib Sengupta, Lucia Coppo, Esha Sircar, Pradeep Mishra, Arne Holmgren
Summary: The S-nitrosylation of the R1 subunit inhibits RNR catalysis, while denitrosylation or an externally supplied peptide containing vicinal dithiols can restore enzyme activity. The Trx system is necessary for regeneration of oxidized vicinal dithiols in the C-terminal tail/peptide, highlighting the importance of C-terminal vicinal dithiols in regenerating the RNR activity of S-nitrosylated R1 subunits.
Article
Biochemistry & Molecular Biology
Surupa Chakraborty, Ankita Choudhuri, Akansha Mishra, Camelia Bhattacharyya, Timothy R. Billiar, Detcho A. Stoyanovsky, Rajib Sengupta
Summary: The interplay between redox-based modification and denitrosylation mechanisms affects gene expression and apoptosis in cells.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2023)
Review
Biology
Yun-Jin Ju, Hye-Won Lee, Ji-Woong Choi, Min-Sik Choi
Summary: Excessive nitrosative stress contributes to neurodegenerative diseases by producing misfolded proteins. The relationship between S-nitrosylated proteins and the accumulation of misfolded proteins was reviewed, with a focus on parkin and PDI. Therapeutic targets for protein misfolding-associated diseases include NOS and GSNOR, in addition to S-nitrosylated target proteins.
Article
Cardiac & Cardiovascular Systems
Somy Yoon, Mira Kim, Hangyeol Lee, Gaeun Kang, Kenneth Bedi, Kenneth B. Margulies, Rajan Jain, Kwang-Il Nam, Hyun Kook, Gwang Hyeon Eom
Summary: The study reveals that neuronal nitric oxide synthase induces diastolic dysfunction by S-nitrosylation of HDAC2, suggesting a new therapeutic platform for refractory heart failure with preserved ejection fraction.
Review
Biochemistry & Molecular Biology
Kifayat Ullah, Emmanuel Zubia, Mahesh Narayan, Jing Yang, Guoqiang Xu
Article
Cell Biology
Liang Zhou, Guoqiang Xu
CELL DEATH & DISEASE
(2019)
Article
Biochemistry & Molecular Biology
Kifayat Ullah, Suping Chen, Jiaqi Lu, XiaoHui Wang, Qing Liu, Yang Zhang, Yaqiu Long, Zhanhong Hu, Guoqiang Xu
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Yukang Yuan, Ying Miao, Liping Qian, Yang Zhang, Chao Liu, Jin Liu, Yibo Zuo, Qian Feng, Tingting Guo, Liting Zhang, Xiangjie Chen, Lincong Jin, Fan Huang, Hongguang Zhang, Wei Zhang, Wei Li, Guoqiang Xu, Hui Zheng
Article
Cell Biology
Ji Geng, Rong Zhang, Xiao Yuan, Haidong Xu, Zhou Zhu, Xinxin Wang, Yan Wang, Guoqiang Xu, Wenjie Guo, Junchao Wu, Zheng-Hong Qin
CELL DEATH & DISEASE
(2020)
Article
Biochemistry & Molecular Biology
Zhanhong Hu, Xiaohui Wang, Dan Li, Lindong Cao, Hongxia Cui, Guoqiang Xu
Summary: High UFBP1 expression increases progression-free survival in advanced gastric cancer patients receiving platinum-based chemotherapy, while UFBP1 knockdown decreases sensitivity of gastric cancer cells to cisplatin. UFBP1 regulates AKR1C protein levels, reactive oxygen species, and Nrf2 activity, enhancing gastric cancer cell sensitivity to cisplatin through the Nrf2/AKR1C axis.
Article
Biochemical Research Methods
Jiaqi Lu, Honglv Jiang, Dan Li, Tao Chen, Yuhong Wang, Zhongjian Pu, Guoqiang Xu
Summary: The study confirmed the inhibitory effect of indisulam on the proliferation of gastric cancer cells, showing that it promotes ubiquitination and degradation of RBM39 by enhancing its interaction with DCAF15. This mechanism highlights the potential of indisulam as a treatment for gastric cancer through proteolysis.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Chemistry, Analytical
Zhiyu Zhang, Xinyu Cheng, Honglv Jiang, Jingyu Gu, Yunfei Yin, Zhijia Shen, Changgang Xu, Zhongjian Pu, Jia-Bin Li, Guoqiang Xu
Summary: The study successfully enriched glycoproteins in 36 urine samples using magnetic ConA nanoparticles, identifying 20 suspicious proteins significantly upregulated in the urine of SCLC patients. Further experiments confirmed that glycosylated cathepsin C and transferrin could serve as potential noninvasive biomarkers for the diagnosis of SCLC patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Xiao-Ou Hou, Hai-Yue Tu, Hai-Chun Qian, Qian Li, Ya-Ping Yang, Guo-Qiang Xu, Fen Wang, Chun-Feng Liu, Ya-Li Wang, Li-Fang Hu
Summary: This study found that exogenous H2S donors can enhance autophagic flux through the AMPK-mTOR signaling pathway, thus reducing the accumulation of alpha-synuclein in dopaminergic cells.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Biochemical Research Methods
Zenghui Chang, Qianqian Duan, Chenyi Yu, Dan Li, Honglv Jiang, Fei Ge, Guoqiang Xu
Summary: This study identified that ADAM12S can enhance the migration of gastric cancer cells by upregulating the glycosylated protein CD146. The findings suggest that ADAM12 could be a potential therapeutic target for the treatment of gastric cancer patients.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jie Zhang, Yijun Yang, Xinhua Li, Gen Li, Takuya Mizukami, Yanli Liu, Yuan Wang, Guoqiang Xu, Heinrich Roder, Li Zhang, Zeng-jie Yang
Summary: PDLIM3 plays a crucial role in ciliogenesis and hedgehog signaling transduction in SHH-MB cells, promoting cell proliferation and tumor growth. It can serve as a molecular marker for defining MB.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemical Research Methods
Xiaohui Wang, Lindong Cao, Honglv Jiang, Liang Zhou, Zhanhong Hu, Guoqiang Xu
Summary: Protein aggregates are important in the development of neurodegenerative diseases, with p62 being a key protein in regulating their formation. A recent discovery shows that the depletion of certain enzymes in the UFM1-conjugation system leads to the accumulation of p62 and the formation of p62 bodies. It is unclear if UfSP1 is involved in the formation of p62 bodies and if its enzymatic activity is required for this process.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Analytical
Jiajia Li, Shuang Yue, Ziyuan Gao, Wenhua Hu, Zhaoliang Liu, Guoqiang Xu, Zhen Wu, Xumin Zhang, Guolin Zhang, Fuliang Qian, Junhong Jiang, Shuang Yang
Summary: RNA modifications, specifically glycosylation, can alter cellular function and lead to differential immune responses. A solid-phase chemoenzymatic method has been developed to specifically recognize glycosylated RNA substrates. The differential glycosylation of small RNAs in cancer cells has been observed, and it affects tumor cell proliferation and survival.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Qihui Lian, Yuan Gao, Qian Li, Xian He, Xiaogang Jiang, Zhongjian Pu, Guoqiang Xu
Article
Oncology
Hui Hou, Dan Li, Junyan Gao, Li Gao, Qin Lu, Yixin Hu, Shuiyan Wu, Xinran Chu, Yanhua Yao, Lin Wan, Jing Ling, Jian Pan, Guoqiang Xu, Shaoyan Hu
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2020)