Article
Chemistry, Analytical
Ming Yan, Haixiao Fang, Xiaoqing Wang, Jiajia Xu, Chengwu Zhang, Li Xu, Lin Li
Summary: Endoplasmic reticulum (ER) stress is involved in diseases like Parkinson's disease, with peroxynitrite being a pivotal factor. A two-photon fluorescence probe, ER-PN, was designed and synthesized for visualizing ER peroxynitrite in PD models. The probe showed rapid and highly selective response to peroxynitrite in vitro, making it a promising tool for imaging peroxynitrite in ER stress-related diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Hua Wei, Xiaochan Tang, Qingxian Chen, Tao Yue, Baoli Dong
Summary: In this study, an ER-targeting fluorescent probe was developed to detect viscosity changes in the endoplasmic reticulum (ER) during ferroptosis. The results showed that erastin-induced ferroptosis led to an increase in ER viscosity, which could be inhibited by Fer-1 and VE.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Applied
Shane M. Hickey, Ian R. D. Johnson, Elena Dallerba, Mark J. Hackett, Massimiliano Massi, Joanna Lazniewska, Lauren A. Thurgood, Frederick M. Pfeffer, Douglas A. Brooks, Trent D. Ashton
Summary: We introduce a highly fluorescent, solvatochromic, 1,8-naphthalimide probe that enables the simultaneous imaging of lipid droplets and endoplasmic reticulum, with distinguishable emission maxima. By delineating organelles based on their emission profiles, we are able to visualize lipid droplet biogenesis and intercellular trafficking events. Furthermore, this probe demonstrates potential histochemical applications by staining myelin lipids in ex vivo brain tissue.
Article
Spectroscopy
Xiuxiu Yue, Jiali Chen, Wenqiang Chen, Benhua Wang, Hui Zhang, Xiangzhi Song
Summary: The endoplasmic reticulum, known as the largest organelle in many eukaryotic cells, plays a vital role in maintaining normal cell function. A novel endoplasmic reticulum-targeting fluorescent probe, ER-CP, has been developed for distinguishing and imaging Cys, Hcy, and GSH with favorable sensitivity and selectivity. ER-CP shows good ER-targeting property and has been successfully applied in imaging these biothiols in HeLa cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biophysics
Li Qiao, Xinxin Shao, Shijie Gao, Zheng Ming, Xianjun Fu, Qingcong Wei
Summary: The endoplasmic reticulum (ER) plays crucial roles in protein synthesis, lipid biosynthesis, and calcium homeostasis, making it an important target for diagnosing and treating diseases. Excessive ER stress (ERS) has a killing effect on malignant cells and can mediate cell apoptosis, providing new perspectives for treatment of diseases, including cancer. Targeting ER and proper modulation of ERS are potential strategies in cancer therapy.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Analytical
Abdul Hadi Mehmood, Baoli Dong, Yaru Lu, Wenhui Song, Yaru Sun, Weiying Lin
Summary: 1,4-Dithiothreitol (DTT) is a crucial reducing agent involved in protein folding and ER homeostasis. Abnormally high levels of DTT can cause ER stress and cell death. A newly designed ER-targeting fluorescent probe, ER-DTT, based on naphthalimide, p-toluenesulfonamide, and sulfoxide, showed good selectivity and sensitivity for imaging DTT in living cells.
ANALYTICAL METHODS
(2021)
Review
Biochemistry & Molecular Biology
Sanaz Dastghaib, P. Sravan Kumar, Sajjad Aftabi, Gautam Damera, Azadeh Dalvand, Adel Sepanjnia, Mohammad Kiumarsi, Mohamad-Reza Aghanoori, Sukhwinder Singh Sohal, Sudharsana R. Ande, Javad Alizadeh, Pooneh Mokarram, Saeid Ghavami, Pawan Sharma, Amir A. Zeki
Summary: Lung cells are exposed to internal and external stressors, leading to disruption of protein homeostasis and activation of the UPR. Dysregulation of the UPR is associated with disease development and various human conditions. Compounds targeting the UPR pathway show potential for future therapeutic interventions.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Pavel E. Z. Klier, Anneliese M. M. Gest, Julia G. Martin, Ryan Roo, Marisol X. Navarro, Lauren Lesiak, Parker E. Deal, Neville Dadina, Jonathan Tyson, Alanna Schepartz, Evan W. Miller
Summary: This study presents the design, synthesis, and application of LUnAR RhoVR, an optical detector for monitoring membrane potential changes in the endoplasmic reticulum (ER) of living cells. By combining a fluorescence probe with molecules that target the ER, voltage changes in the ER can be accurately observed. The study validates the electroneutrality of calcium release and provides direct evidence of the ER potential response to plasma membrane potential changes. This research offers a new tool for exploring organelle physiology.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Tienan Zang, Shudong Wang, Sa Su, Mengxu Gao, Qianqian Chen, Chenlu Liang, Jing Jing, Rubo Zhang, Xiaoling Zhang
Summary: A new fluorescent probe was designed to detect the key enzyme SQLE in living cells, showing specific response and ability to rapidly assess antifungal drug susceptibility. The probe provides a powerful tool for monitoring the content and distribution of SQLE.
ANALYTICAL CHEMISTRY
(2021)
Article
Biology
Michael J. Rigby, Alexis J. Lawton, Gulpreet Kaur, Varuna C. Banduseela, William E. Kamm, Aparna Lakkaraju, John M. Denu, Luigi Puglielli
Summary: Rigby et al. utilize Atase1 or Atase2-deficient transgenic mice to demonstrate the distinct roles of these endoplasmic reticulum acetyltransferases in regulating reticulophagy and macroautophagy. Their findings highlight Atase1 as a potential translational target for diseases characterized by toxic protein aggregation in the secretory pathway.
COMMUNICATIONS BIOLOGY
(2021)
Review
Chemistry, Organic
Jiarun Lin, Kylie Yang, Elizabeth J. New
Summary: Fluorescent tools are crucial for studying organelles' distinct chemical microenvironments. While many fluorescent tools have been developed, most only target a few organelles, leaving less-studied organelles with limited robust targeting strategies. Developing new, robust strategies is essential for expanding the effective study of organelles and creating new tools.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Review
Cell Biology
Elina Ikonen, Xin Zhou
Summary: This article summarizes the asymmetric distribution of cholesterol in mammalian cells and its co-transport with other lipids, discussing lipid transfer proteins at membrane contacts and membrane transport. It also reviews the physiological evidence related to this process.
DEVELOPMENTAL CELL
(2021)
Article
Biochemistry & Molecular Biology
Hyun Ku Yeo, Tae Hyun Park, Hee Yeon Kim, Hyonchol Jang, Jueun Lee, Geum-Sook Hwang, Seong Eon Ryu, Si Hoon Park, Hyun Kyu Song, Hyun Seung Ban, Hye-Jin Yoon, Byung Il Lee
Summary: The study revealed the functions of PTPIP51 in phospholipid binding/transfer, particularly of phosphatidic acid, in cells. Depletion of PTPIP51 reduces the mitochondrial cardiolipin level, and the interaction between PTPIP51 and VAPB is mediated by specific structural motifs.
Article
Chemistry, Analytical
Zhao Wang, Jiankang Gong, Pengzhan Wang, Jianhua Xiong, Fan Zhang, Zhiqiang Mao
Summary: This study developed a novel activatable AIE fluorescent probe (DPPO-PN) for the detection of ONOO- levels in rheumatoid arthritis (RA). The probe showed excellent sensing abilities for ONOO- and could capture and visualize the fluctuations of intracellular ONOO- levels in real-time using confocal imaging. Moreover, the probe enabled intravital imaging of ONOO- in LPS-induced and CFA-induced RA mouse models.
Article
Environmental Sciences
Junjun Peng, Xueyan Dai, Huiqin Fan, Chenghong Xing, Yu Zhuang, Xiaona Gao, Huabin Cao, Guoliang Hu, Fan Yang
Summary: Copper (Cu) at high levels can cause harm to the host physiology, specifically resulting in nephrotoxicity, although the exact mechanism remains unclear. This study explored the relationship between mitochondria-associated ER membranes (MAM) and Cu-induced kidney mitochondrial dysfunction and ER stress. The findings revealed that excessive Cu disrupted MAM integrity, leading to MAM dysfunction, mitochondrial dysfunction, and ER stress, contributing to Cu-induced nephrotoxicity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Applied
Sahishna Phaniraj, Zhe Gao, Digamber Rane, Blake R. Peterson
Article
Microbiology
Jennifer R. Klaus, Jacqueline Deay, Benjamin Neuenswander, Wyatt Hursh, Zhe Gao, Tiffany Bouddhara, Todd D. Williams, Justin Douglas, Kyle Monize, Patricia Martins, Charlotte Majerczyk, Mohammad R. Seyedsayamdost, Blake R. Peterson, Mario Rivera, Josephine R. Chandler
JOURNAL OF BACTERIOLOGY
(2018)
Article
Immunology
Samir El Qaidi, Congrui Zhu, Peter McDonald, Anuradha Roy, Pradip Kumar Maity, Digamber Rane, Chamani Perera, Philip R. Hardwidge
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2018)
Article
Chemistry, Multidisciplinary
Achala N. D. Punchi Hewage, Huili Yao, Baskar Nammalwar, Krishna Kumar Gnanasekaran, Scott Lovell, Richard A. Bunce, Kate Eshelman, Sahishna M. Phaniraj, Molly M. Lee, Blake R. Peterson, Kevin P. Battaile, Allen B. Reitz, Mario Rivero
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
Kelsey E. Knewtson, Chamani Perera, David Hymel, Zhe Gao, Molly M. Lee, Blake R. Peterson
Article
Biochemistry & Molecular Biology
Ruben De Coen, Lutz Nuhn, Chamani Perera, Maria Arista-Romero, Martijn D. P. Risseeuw, Alec Freyn, Raffael Nachbagauer, Lorenzo Albertazzi, Serge Van Calenbergh, David A. Spiegel, Blake R. Peterson, Bruno G. De Geest
Article
Microbiology
Saida Benomar, Patrick Lansdon, Aaron M. Bender, Blake R. Peterson, Josephine R. Chandler, Brian D. Ackley
Article
Biochemistry & Molecular Biology
Giuseppe Caruso, Cristina Benatti, Nicolo Musso, Claudia G. Fresta, Annamaria Fidilio, Giorgia Spampinato, Nicoletta Brunello, Claudio Bucolo, Filippo Drago, Susan M. Lunte, Blake R. Peterson, Fabio Tascedda, Filippo Caraci
Summary: The study showed that carnosine can protect macrophages from cell death and apoptosis induced by Aβ 1-42 oligomers, decrease oxidative stress, enhance macrophage phagocytic activity, and rescue fractalkine receptor CX3CR1. These new findings support the therapeutic potential of carnosine in the context of AD pathology, highlighting its multimodal mechanism of action.
Article
Chemistry, Medicinal
Erick J. Carlson, Himanshu Savardekar, Xiaojun Hu, Gabriella Lapurga, Courtney Johnson, Steven H. Sun, William E. Carson, Blake R. Peterson
Summary: Myeloid-derived suppressor cells (MDSCs) are immature myeloid cells that expand dramatically in cancer patients, leading to immunosuppression. MDSCs mediate immunosuppression by producing peroxynitrite (PNT), which inactivates immune effector cells. A fluorescent sensor called PS3 allows direct detection of PNT, and using this method, it was found that cancer mice and melanoma patients have higher levels of PNT due to increased numbers of MDSCs. The kinase inhibitor dasatinib can block PNT production by inhibiting phagocytosis and reducing MDSC numbers.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Zhe Gao, Krishna K. Sharma, Angelo E. Andres, Brandon Walls, Fadel Boumelhem, Zachary R. Woydziak, Blake R. Peterson
Summary: Fluorinated analogues of pyronin B, a fluorophore, were synthesized and shown to react reversibly with primary amines. These analogues undergo oxidation in the presence of proteins, yielding fluorescent pyronins, and this oxidation step can be enhanced by blue light irradiation. The fluorinated analogue 2,7-difluoropyronin B was found to localize in mitochondria in living HeLa cells and depolarize the mitochondria upon blue laser excitation. This photochemical control of mitochondrial biology is attributed to the enhanced cellular production of reactive oxygen species.
RSC MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Molly M. Lee, Zhe Gao, Blake R. Peterson
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)
Article
Chemistry, Multidisciplinary
Molly M. Lee, Blake R. Peterson
Article
Chemistry, Multidisciplinary
Thilanga N. Pahattuge, J. Matt Jackson, Rane Digamber, Harshani Wijerathne, Virginia Brown, Malgorzata A. Witek, Chamani Perera, Richard S. Givens, Blake R. Peterson, Steven A. Soper
CHEMICAL COMMUNICATIONS
(2020)