Review
Cell Biology
Amity F. Eaton, Maria Merkulova, Dennis Brown
Summary: The primary function of H+-ATPase is to create an electrochemical proton gradient across eukaryotic cell membranes to energize cellular processes. It acidifies intracellular vesicles and organelles for essential cell biological events. Additionally, V-ATPases play central roles in normal and pathophysiological processes that impact human health, influencing areas such as cancer, neurodegenerative diseases, and sensory perception.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Paula Gutierrez-Perez, Emilio M. Santillan, Thomas Lendl, Jingkui Wang, Anna Schrempf, Thomas L. Steinacker, Mila Asparuhova, Marlene Brandstetter, David Haselbach, Luisa Cochella
Summary: The study reveals the vulnerability of muscle cells by miR-1, which represses multiple subunits of the V-ATPase complex, impacting muscle function. This sheds light on a previously unknown role for microRNAs in regulating protein complexes and V-ATPase assembly as a conserved muscle cell vulnerability.
Article
Biochemistry & Molecular Biology
Qing Tang, Mingming Liu, Yang Liu, Ran-Der Hwang, Tao Zhang, Jiou Wang
Summary: The study identified NDST3 as a potent regulator of lysosomal functions, with implications in neurodegenerative diseases associated with C9orf72. Lack of NDST3 increases organelle acidification, while its downregulation exacerbates protein toxicity linked to C9orf72.
Review
Cell Biology
Johannes Westman, Sergio Grinstein
Summary: The ability of phagosomes to halt microbial growth is closely linked to their ability to acidify their luminal pH, but certain pathogens can survive and replicate inside phagosomes by targeting the pH-regulatory machinery of host cells to survive or escape.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Plant Sciences
Thorsten Seidel
Summary: V-ATPase is the dominant proton pump in plant cells, playing crucial roles in cytosolic pH homeostasis, endomembrane transport processes, and stress responses. Its complex structure and multiple subunit isoforms provide structural flexibility, but also require sophisticated assembly machinery and transport routes in cells.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Endocrinology & Metabolism
Jiefu Wang, Yasuhiro Onogi, Martin Krueger, Josef Oeckl, Ruth Karlina, Inderjeet Singh, Stefanie M. Hauck, Regina Feederle, Yongguo Li, Siegfried Ussar
Summary: PAT2 serves as an important sensor of extracellular amino acids and regulates lysosomal acidification in brown adipocytes. Loss or overexpression of PAT2 affects lysosomal acidification and starvation-induced S6K re-phosphorylation, as PAT2 facilitates the assembly of lysosomal vATPase by recruiting the cytoplasmic V1 subunit to the lysosome.
MOLECULAR METABOLISM
(2022)
Article
Multidisciplinary Sciences
Rong Wang, Yu Qin, Xiao-Song Xie, Xiaochun Li
Summary: This study identifies mEAK7 as an endogenous modulator of V-ATPase and determines its structure in complex with V-ATPase. The findings suggest that mEAK7 may function as an inhibitor of V-ATPase and potentially link mTOR signaling with V-ATPase activity.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Luis Mauricio T. R. Lima, Thayna Sisnande
Summary: Amyloids, organized suprastructural polypeptide arrangements, play a role in aging-related degenerative diseases. Non-genetic factors, particularly transition metals, contribute to the regulation of amyloidogenic processes. These processes have replicative nature and systemic distribution, indicating their metastatic nature. Amyloidosis acts as nutrient sensors and may participate in epigenetic metabolic programming and viability determination.
Article
Biochemistry & Molecular Biology
Hanlin Wang, John L. Rubinstein
Summary: Vacuolar-type ATPases (V-ATPases) play a crucial role in acidifying intracellular compartments and have been linked to various diseases. Recent studies have revealed the structures and mechanisms of V-ATPases, as well as their assembly, regulation, and modulation by proteins and small molecules. This review highlights these recent developments.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Hanlin Wang, Stephanie A. Bueler, John L. Rubinstein
Summary: Before the assembly of V-ATPase, Vma12-22p and Vma21p play a role in preventing the acidification of the endoplasmic reticulum, ensuring that acidification only occurs in the intended cellular targets.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Yong Zi Tan, Yazan M. Abbas, Jing Ze Wu, Di Wu, Kristine A. Keon, Geoffrey G. Hesketh, Stephanie A. Bueler, Anne-Claude Gingras, Carol Robinson, Sergio Grinstein, John L. Rubinstein
Summary: CryoEM analysis revealed an interaction between mEAK-7 and V-ATPase, which is disrupted by ATP-induced rotation. Exogenous mEAK-7 does not inhibit V-ATPase activity unlike its yeast homolog.
LIFE SCIENCE ALLIANCE
(2022)
Article
Cell Biology
Michelle E. Maxson, Yazan M. Abbas, Jing Ze Wu, Jonathan D. Plumb, Sergio Grinstein, John L. Rubinstein
Summary: This study shows that proton-pumping vacuolar H+ ATPases (V-ATPases) are responsible for the acidic environment in secretory and endocytic organelles. The researchers developed a new probe derived from SidK protein to visualize and quantify V-ATPases with high specificity. They found that the density of V-ATPases increases during phagosome maturation and their distribution varies in lysosomes depending on their subcellular localization.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michael C. Jaskolka, Maureen Tarsio, Anne M. Smardon, Md Murad Khan, Patricia M. Kane
Summary: The vacuolar H+-ATPase (V-ATPase) is a proton pump responsible for acidifying organelles in cells. The RAVE complex plays a crucial role in the reassembly of V-ATPases through the rapid and reversible disassembly and reassembly processes. Native RAVE and RAVE-V1 complexes purified using an inducible overexpression system contain substoichiometric levels of subunit C, suggesting their importance in V-ATPase reassembly.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Plant Sciences
Ying Wang, Huimin Feng, Jia Du, Xinxin Liu, Haiya Wang, Xiaoli Dai, Guohua Xu, Ling Yu
Summary: In this study, it was found that HtVHA-c and HtNHX2 have a synergistic function in regulating the pH of endosomes, improving plant mineral nutrition and salt tolerance.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Review
Biochemistry & Molecular Biology
Farzana Tuli, Patricia M. Kane
Summary: Vacuolar H+-ATPases (V-ATPases) are multi-subunit enzymes that acidify organelles and export protons in eukaryotic cells. The largest membrane subunit, the a-subunit, interacts with other subunits and bridges the V-1 and V-o subcomplexes. Multiple isoforms of the a-subunit exist, allowing for specific interactions and regulation. Understanding the regulatory mechanisms of V-ATPases, specifically the a-subunit isoforms, is important for the treatment of diseases associated with altered V-ATPase function.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Meeting Abstract
Oncology
Patricia E. Mccoon, Rich Woessner, Shaun Grosskurth, Chris Womack, Mason Yamashita, Gene Hung, Robert MacLeod, Kirsten Bell, Mike Collins, Rachel DuPont, Vivian Jacobs, Michele Johnstone, Margaret Veldman-Jones, Paul Lyne
Meeting Abstract
Oncology
Rich Woessner, Patricia McCoon, Kirsten Bell, Rachel DuPont, Mike Collins, Deborah Lawson, Prasad Nadella, Lourdes Pablo, Corinne Reimer, Vasu Sah, Paul Lyne
CANCER IMMUNOLOGY RESEARCH
(2015)
Article
Oncology
Garrett W. Rhyasen, Maureen M. Hattersley, Yi Yao, Austin Dulak, Wenxian Wang, Philip Petteruti, Ian L. Dale, Scott Boiko, Tony Cheung, Jingwen Zhang, Shenghua Wen, Lillian Castriotta, Deborah Lawson, Michael Collins, Larry Bao, Miika J. Ahdesmaki, Graeme Walker, Greg O'Connor, Tammie C. Yeh, Alfred A. Rabow, Jonathan R. Dry, Corinne Reimer, Paul Lyne, Gordon B. Mills, Stephen E. Fawell, Michael J. Waring, Michael Zinda, Edwin Clark, Huawei Chen
MOLECULAR CANCER THERAPEUTICS
(2016)
Article
Oncology
Brendon Ladd, Anne Marie Mazzola, Teeru Bihani, Zhongwu Lai, James Bradford, Michael Collins, Evan Barry, Anne U. Goeppert, Hazel M. Weir, Kelly Hearne, Jonathan G. Renshaw, Morvarid Mohseni, Elaine Hurt, Sanjoo Jalla, Haifeng Bao, Robert Hollingsworth, Corinne Reimer, Michael Zinda, Stephen Fawell, Celina M. D'Cruz
Article
Oncology
Wei Yang, Muneeb Ahmed, Beenish Tasawwar, Tatyana Levchenko, Rupa R. Sawant, Michael Collins, Sabina Signoretti, Vladimir Torchilin, S. Nahum Goldberg
INTERNATIONAL JOURNAL OF HYPERTHERMIA
(2011)
Article
Chemistry, Medicinal
Maria-Elena Theoclitou, Brian Aquila, Michael H. Block, Patrick J. Brassil, Lillian Castriotta, Erin Code, Michael P. Collins, Audrey M. Davies, Tracy Deegan, Jayachandran Ezhuthachan, Sandra Filla, Ellen Freed, Haiqing Hu, Dennis Huszar, Muthusamy Jayaraman, Deborah Lawson, Paula M. Lewis, Murali V. P. Nadella, Vibha Oza, Maniyan Padmanilayam, Timothy Pontz, Lucienne Ronco, Daniel Russell, David Whitston, Xiaolan Zheng
JOURNAL OF MEDICINAL CHEMISTRY
(2011)
Article
Multidisciplinary Sciences
Wenliang Lia, Nanping Ai, Suming Wang, Nandita Bhattacharya, Vladimir Vrbanac, Michael Collins, Sabina Signoretti, Yanhui Hu, Frederick M. Boyce, Karsten Gravdal, Ole J. Halvorsen, Hawa Nalwoga, Lars A. Akslen, Ed Harlow, Randolph S. Watnick
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Oncology
Scott A. Ezell, Michele Mayo, Teeru Bihani, Suprawee Tepsuporn, Suping Wang, Melissa Passino, Shaun E. Grosskurth, Mike Collins, Julie Parmentier, Corinne Reimer, Kate F. Byth
Article
Oncology
Mary E. Loveless, Deborah Lawson, Michael Collins, Murali V. Prasad Nadella, Corinne Reimer, Dennis Huszar, Jane Halliday, John C. Waterton, John C. Gore, Thomas E. Yankeelov
Article
Biochemistry & Molecular Biology
Christina M. McGuire, Michael P. Collins, GeHong Sun-Wada, Yoh Wade, Michael Forgac
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Michael P. Collins, Laura A. Stransky, Michael Forgac
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Oncology
Theresa A. Proia, Maneesh Singh, Richard Woessner, Larissa Carnevalli, Gayathri Bommakanti, Lukasz Magiera, Srimathi Srinivasan, Shaun Grosskurth, Mike Collins, Chris Womack, Matthew Griffin, Minwei Ye, Susan Cantin, Deanna Russell, Mingchao Xie, Adina Hughes, Nanhua Deng, Deanna A. Mele, Stephen Fawell, Simon Barry, Corinne Reimer, J. Carl Barrett, Patricia McCoon
CLINICAL CANCER RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Kevin Su, Michael P. Collins, Christina M. McGuire, Mohammed A. Alshagawi, Mariam K. Alamoudi, Zhen Li, Michael Forgac
Summary: The a4 isoform of the V-ATPase plays a significant role in the migration and invasion of breast cancer cells, as well as in the growth of tumors in vivo. Targeting this isoform may represent a novel therapeutic strategy to limit breast cancer growth and metastasis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Naomi Laing, Brenda McDermott, Shenghua Wen, David Yang, Deborah Lawson, Mike Collins, Corinne Reimer, Peter A. Hall, Harriet Andersen, Michael Snaith, Xin Wang, Vahe Bedian, Zhu A. Cao, David Blakey
MOLECULAR PHARMACOLOGY
(2013)