Optimal fluorescent protein tags for quantifying protein oligomerization in living cells

Full-length cellular β-secretase has a trimeric subunit stoichiometry, and its sulfur-rich transmembrane interaction site modulates cytosolic copper compartmentalization

(2017) Filip Liebsch et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phosphatidylserine Lateral Organization Influences the Interaction of Influenza Virus Matrix Protein 1 with Lipid Membranes

(2017) Sara Bobone et al.   JOURNAL OF VIROLOGY

Direct evidence of amyloid precursor–like protein 1 trans interactions in cell–cell adhesion platforms investigated via fluorescence fluctuation spectroscopy

(2017) Valentin Dunsing et al.   MOLECULAR BIOLOGY OF THE CELL

Systematic characterization of maturation time of fluorescent proteins in living cells

(2017) Enrique Balleza et al.   NATURE METHODS

Quantifying transcription factor–DNA binding in single cells in vivo with photoactivatable fluorescence correlation spectroscopy

(2017) Ziqing Winston Zhao et al.   Nature Protocols

Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity

(2017) Yi Shen et al.   PLoS One

Three-Fragment Fluorescence Complementation Coupled with Photoactivated Localization Microscopy for Nanoscale Imaging of Ternary Complexes

(2016) Minghai Chen et al.   ACS Nano

Amyloid precursor-like protein 1 (APLP1) exhibits stronger zinc-dependent neuronal adhesion than amyloid precursor protein and APLP2

(2016) Magnus C. Mayer et al.   JOURNAL OF NEUROCHEMISTRY

mScarlet: a bright monomeric red fluorescent protein for cellular imaging

(2016) Daphne S Bindels et al.   NATURE METHODS

Quantitative assessment of fluorescent proteins

(2016) Paula J Cranfill et al.   NATURE METHODS

EGFP oligomers as natural fluorescence and hydrodynamic standards

(2016) György Vámosi et al.   Scientific Reports

Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting

(2016) Bryce T. Bajar et al.   Scientific Reports

A Set of Homo-Oligomeric Standards Allows Accurate Protein Counting

(2015) Kieran Finan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Spatial Intensity Distribution Analysis Reveals Abnormal Oligomerization of Proteins in Single Cells

(2015) Antoine G. Godin et al.   BIOPHYSICAL JOURNAL

Live-cell observation of cytosolic HIV-1 assembly onset reveals RNA-interacting Gag oligomers

(2015) Jelle Hendrix et al.   JOURNAL OF CELL BIOLOGY

One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy

(2015) Franziska Fricke et al.   Scientific Reports

Dual-color dual-focus line-scanning FCS for quantitative analysis of receptor-ligand interactions in living specimens

(2015) René M. Dörlich et al.   Scientific Reports

Deciphering the Subunit Composition of Multimeric Proteins by Counting Photobleaching Steps

(2014) Ryan J. Arant et al.   CHEMPHYSCHEM

Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein

(2014) Jun Chu et al.   NATURE METHODS

Quantitative Measurement of Brightness from Living Cells in the Presence of Photodepletion

(2014) Kwang-Ho Hur et al.   PLoS One

Near-infrared fluorescent proteins for multicolor in vivo imaging

(2013) Daria M Shcherbakova et al.   NATURE METHODS

N-Way FRET Microscopy of Multiple Protein-Protein Interactions in Live Cells

(2013) Adam D. Hoppe et al.   PLoS One

Factors Affecting the Quantification of Biomolecular Interactions by Fluorescence Cross-Correlation Spectroscopy

(2012) Yong Hwee Foo et al.   BIOPHYSICAL JOURNAL

Automating Single Subunit Counting of Membrane Proteins in Mammalian Cells

(2012) Hugo McGuire et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cross-Correlated Fluctuation Analysis Reveals Phosphorylation-Regulated Paxillin-FAK Complexes in Nascent Adhesions

(2011) Colin K. Choi et al.   BIOPHYSICAL JOURNAL

Number and brightness image analysis reveals ATF-induced dimerization kinetics of uPAR in the cell membrane

(2011) Christian Hellriegel et al.   FASEB JOURNAL

Metadata matters: access to image data in the real world

(2010) Melissa Linkert et al.   JOURNAL OF CELL BIOLOGY

Distribution of resting and ligand-bound ErbB1 and ErbB2 receptor tyrosine kinases in living cells using number and brightness analysis

(2010) P. Nagy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Detecting Protein Complexes in Living Cells from Laser Scanning Confocal Image Sequences by the Cross Correlation Raster Image Spectroscopy Method

(2009) Michelle A. Digman et al.   BIOPHYSICAL JOURNAL

Fluorescence Fluctuation Spectroscopy of mCherry in Living Cells

(2009) Bin Wu et al.   BIOPHYSICAL JOURNAL

Accurate Determination of Membrane Dynamics with Line-Scan FCS

(2009) Jonas Ries et al.   BIOPHYSICAL JOURNAL

Lateral Distribution of the Transmembrane Domain of Influenza Virus Hemagglutinin Revealed by Time-resolved Fluorescence Imaging

(2009) Silvia Scolari et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms

(2009) Jonas Ries et al.   NATURE METHODS

Stoichiometry of molecular complexes at adhesions in living cells

(2009) M. A. Digman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bimolecular Fluorescence Complementation (BiFC) Analysis as a Probe of Protein Interactions in Living Cells

(2008) Tom K. Kerppola   Annual Review of Biophysics

Dark States in Monomeric Red Fluorescent Proteins Studied by Fluorescence Correlation and Single Molecule Spectroscopy

(2008) Jelle Hendrix et al.   BIOPHYSICAL JOURNAL

Visualization of ternary complexes in living cells by using a BiFC-based FRET assay

(2008) Y John Shyu et al.   Nature Protocols

SAM Domain-Based Protein Oligomerization Observed by Live-Cell Fluorescence Fluctuation Spectroscopy

(2008) Brian D. Slaughter et al.   PLoS One

Mapping the Number of Molecules and Brightness in the Laser Scanning Microscope

(2007) Michelle A. Digman et al.   BIOPHYSICAL JOURNAL