Deciphering the Subunit Composition of Multimeric Proteins by Counting Photobleaching Steps

A cell-free assay to determine the stoichiometry of plasma membrane proteins

(2016) Cesar Trigo et al.   BIOTECHNIQUES

Single-Molecule Imaging of the Oligomer Formation of the Nonhexameric Escherichia coli UvrD Helicase

(2013) Hiroaki Yokota et al.   BIOPHYSICAL JOURNAL

Structural and Functional Similarities of Calcium Homeostasis Modulator 1 (CALHM1) Ion Channel with Connexins, Pannexins, and Innexins

(2013) Adam P. Siebert et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Inferring subunit stoichiometry from single molecule photobleaching

(2013) Keegan E. Hines   JOURNAL OF GENERAL PHYSIOLOGY

The Conserved Tetrameric Subunit Stoichiometry of Slc26 Proteins

(2013) Richard Hallworth et al.   MICROSCOPY AND MICROANALYSIS

Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization

(2013) Q. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

AMPA receptor/TARP stoichiometry visualized by single-molecule subunit counting

(2013) P. Hastie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ABCA1 dimer-monomer interconversion during HDL generation revealed by single-molecule imaging

(2013) K. O. Nagata et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-molecule superresolution imaging allows quantitative analysis of RAF multimer formation and signaling

(2013) X. Nan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Counting Fluorescent Dye Molecules on DNA Origami by Means of Photon Statistics

(2013) Anton Kurz et al.   Small

Single-molecule photobleaching reveals increased MET receptor dimerization upon ligand binding in intact cells

(2013) Marina S Dietz et al.   BMC Biophysics

β-Amyloid (1–40) Peptide Interactions with Supported Phospholipid Membranes: A Single-Molecule Study

(2012) Hao Ding et al.   BIOPHYSICAL JOURNAL

Dynamics of Major Histocompatibility Complex Class I Association with the Human Peptide-loading Complex

(2012) Michaela S. Panter et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Automating Single Subunit Counting of Membrane Proteins in Mammalian Cells

(2012) Hugo McGuire et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Stoichiometry of the Human Glycine Receptor Revealed by Direct Subunit Counting

(2012) N. Durisic et al.   JOURNAL OF NEUROSCIENCE

Dynamic Association of ORCA with Prereplicative Complex Components Regulates DNA Replication Initiation

(2012) Z. Shen et al.   MOLECULAR AND CELLULAR BIOLOGY

Piezo proteins are pore-forming subunits of mechanically activated channels

(2012) Bertrand Coste et al.   NATURE

A simple, versatile method for GFP-based super-resolution microscopy via nanobodies

(2012) Jonas Ries et al.   NATURE METHODS

Single-molecule pull-down for studying protein interactions

(2012) Ankur Jain et al.   Nature Protocols

One influenza virus particle packages eight unique viral RNAs as shown by FISH analysis

(2012) Y.-y. Chou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure and stoichiometry of an accessory subunit TRIP8b interaction with hyperpolarization-activated cyclic nucleotide-gated channels

(2012) J. R. Bankston et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plasticity of the asialoglycoprotein receptor deciphered by ensemble FRET imaging and single-molecule counting PALM imaging

(2012) M. Renz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-molecule analysis of fluorescently labeled G-protein-coupled receptors reveals complexes with distinct dynamics and organization

(2012) D. Calebiro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular mechanism of the assembly of an acid-sensing receptor ion channel complex

(2012) Yong Yu et al.   Nature Communications

Assembly Stoichiometry of the GluK2/GluK5 Kainate Receptor Complex

(2012) Andreas Reiner et al.   Cell Reports

Single-molecule imaging revealed enhanced dimerization of transforming growth factor β type II receptors in hypertrophic cardiomyocytes

(2011) Kangmin He et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Calmodulin overexpression does not alter Cav1.2 function or oligomerization state

(2011) Felix Findeisen et al.   Channels

Single Molecule Fluorescence Study of theBacillus thuringiensisToxin Cry1Aa Reveals Tetramerization

(2011) Nicolas Groulx et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

SUMO modification of cell surface Kv2.1 potassium channels regulates the activity of rat hippocampal neurons

(2011) Leigh D. Plant et al.   JOURNAL OF GENERAL PHYSIOLOGY

Prestin in HEK cells is an obligate tetramer

(2011) Richard Hallworth et al.   JOURNAL OF NEUROPHYSIOLOGY

Probing cellular protein complexes using single-molecule pull-down

(2011) Ankur Jain et al.   NATURE

Functional Architecture of Olfactory Ionotropic Glutamate Receptors

(2011) Liliane Abuin et al.   NEURON

Single-Molecule Analysis of PIP2;1 Dynamics and Partitioning Reveals Multiple Modes of Arabidopsis Plasma Membrane Aquaporin Regulation

(2011) X. Li et al.   PLANT CELL

Sensing cooperativity in ATP hydrolysis for single multisubunit enzymes in solution

(2011) Y. Jiang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Two synaptobrevin molecules are sufficient for vesicle fusion in central nervous system synapses

(2011) R. Sinha et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Subunit stoichiometry of human Orai1 and Orai3 channels in closed and open states

(2011) A. Demuro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Counting Bungarotoxin Binding Sites of Nicotinic Acetylcholine Receptors in Mammalian Cells with High Signal/Noise Ratios

(2010) Paul D. Simonson et al.   BIOPHYSICAL JOURNAL

Monomeric type I and type III transforming growth factor-β receptors and their dimerization revealed by single-molecule imaging

(2010) Wei Zhang et al.   CELL RESEARCH

Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching

(2010) Tchern Lenn et al.   CHEMICAL COMMUNICATIONS

Stoichiometry of a regulatory splicing complex revealed by single-molecule analyses

(2010) Dmitry Cherny et al.   EMBO JOURNAL

Multiple C-terminal tail Ca2+/CaMs regulate CaV1.2 function but do not mediate channel dimerization

(2010) Eun Young Kim et al.   EMBO JOURNAL

Aquaporin-4 (AQP4) Associations and Array Dynamics Probed by Photobleaching and Single-molecule Analysis of Green Fluorescent Protein-AQP4 Chimeras

(2010) Masato Tajima et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Resting State Orai1 Diffuses as Homotetramer in the Plasma Membrane of Live Mammalian Cells

(2010) Josef Madl et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Experimental approach to extend the range for counting fluorescent molecules based on photon-antibunching

(2010) Haisen Ta et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Near-Membrane Dynamics and Capture of TRPM8 Channels within Transient Confinement Domains

(2010) Luis A. Veliz et al.   PLoS One

One SUMO is sufficient to silence the dimeric potassium channel K2P1

(2010) L. D. Plant et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stoichiometry of the KCNQ1 - KCNE1 ion channel complex

(2010) K. Nakajo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stoichiometry and Architecture of Active DNA Replication Machinery in Escherichia coli

(2010) R. Reyes-Lamothe et al.   SCIENCE

Single-Molecule Imaging of a Fluorescent Unnatural Amino Acid Incorporated Into Nicotinic Receptors

(2009) Rigo Pantoja et al.   BIOPHYSICAL JOURNAL

Determination of the Oligomer Size of Amyloidogenic Protein β-Amyloid(1–40) by Single-Molecule Spectroscopy

(2009) Hao Ding et al.   BIOPHYSICAL JOURNAL

Single-molecule imaging reveals transforming growth factor- -induced type II receptor dimerization

(2009) W. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural and molecular basis of the assembly of the TRPP2/PKD1 complex

(2009) Yong Yu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Monitoring the earliest amyloid-β oligomers via quantized photobleaching of dye-labeled peptides

(2008) Kyle D. Dukes et al.   ANALYTICAL BIOCHEMISTRY

Monomeric CFTR in Plasma Membranes in Live Cells Revealed by Single Molecule Fluorescence Imaging

(2008) Peter M. Haggie et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The CRAC channel consists of a tetramer formed by Stim-induced dimerization of Orai dimers

(2008) Aubin Penna et al.   NATURE

The Voltage-Gated Proton Channel Hv1 Has Two Pores, Each Controlled by One Voltage Sensor

(2008) Francesco Tombola et al.   NEURON

Rules of engagement for NMDA receptor subunits

(2008) M. H. Ulbrich et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Functional stoichiometry of the unitary calcium-release-activated calcium channel

(2008) W. Ji et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA