Review
Medicine, General & Internal
Sabina Ugovsek, Miran Sebestjen
Summary: PCSK9 monoclonal antibodies reduce LDL cholesterol levels and moderately decrease Lp(a) levels by upregulating LDL receptors. They also have the ability to decrease platelet aggregation and activation, increase platelet responsiveness to acetylsalicylic acid, decrease the incidence of venous thromboembolism, and play a role in reducing inflammation and improving endothelial function.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Claire S. Whyte, Gael B. Morrow, Julia S. Gauer, Samantha J. Montague, Philip L. R. Nicolson
Summary: The 2023 annual meeting of the British Society for Haemostasis and Thrombosis (BSHT) was held in Birmingham, United Kingdom, focusing on novel therapeutics and emerging technology. This article discusses the exciting research presented at the meeting.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Michael G. McCoy, Daniel Perez-Cremades, Nathan Belkin, Wenhui Peng, Bofang Zhang, Jingshu Chen, Madhur Sachan, A. K. M. Khyrul Wara, Rulin Zhuang, Henry S. Cheng, Mark W. Feinberg
Summary: This study demonstrates that a 4-miRNA cassette can reprogram vascular smooth muscle cells into endothelial cells, and these induced cells exhibit high similarity to native endothelial cells. Injected induced cells even restore blood flow faster than conventional endothelial cells in a mouse model, suggesting the potential of this approach for vascular disease therapy.
Article
Biochemistry & Molecular Biology
Natalie Jia Ying Yeo, Vanessa Wazny, Nhi Le Uyen Nguyen, Chun-Yi Ng, Kan Xing Wu, Qiao Fan, Chui Ming Gemmy Cheung, Christine Cheung
Summary: Age-related macular degeneration is a common cause of visual impairment in older populations worldwide. Wet AMD, the most common subtype, is characterized by pathological angiogenesis and is typically treated with anti-vascular endothelial growth factor therapy. However, long-term prevention of visual decline is still a challenge. Researchers have identified potential VEGF-independent pathways involved in wet AMD pathogenesis, including extracellular matrix remodeling, interleukin signaling, and amino acid metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Ke Ma, Sitong Liu, Hongyue Liang, Guan Wang, Tianyu Wang, Shuya Luo, Kuan Gao, Hui Wang, Mei Liu, Lichuan Bai, Qinghuan Xiao
Summary: This study reveals a novel mechanism of cholesterol-mediated TMEM16A inhibition, by which cholesterol reduces TMEM16A expression via DNMT1-mediated methylation and directly inhibits channel activities. TMEM16A channel inhibition promotes endothelial cell angiogenesis.
JOURNAL OF ADVANCED RESEARCH
(2021)
Review
Cell & Tissue Engineering
Sarah E. J. Chambers, Varun Pathak, Edoardo Pedrini, Lou Soret, Nicolas Gendron, Coralie L. Guerin, Alan W. Stitt, David M. Smadja, Reinhold J. Medina
Summary: Ischemic vascular disease is a major global cause of mortality and morbidity, and the therapeutic goal of regenerating blood vessels in perfusion-deficient tissues through delivering endothelial stem/progenitor cells has strong scientific basis. However, challenges and contradictions in clinical trials have highlighted the difficulties in developing endothelial stem cell therapies. Emerging evidence raises questions about the traditional view of bone marrow as the source of these cells and suggests the vascular wall as the niche for tissue-resident endothelial stem cells.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Isabel Vidal, Elena Fernandez-Florido, Ana Dacil Marrero, Laura Castilla, Ana R. Quesada, Beatriz Martinez-Poveda, Miguel Angel Medina
Summary: The potential of dimethyl itaconate as an anti-angiogenic compound was evaluated in this study, and the results indicate that it interferes with multiple key steps of the angiogenic process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Longwei Liu, Yunjia Qu, Leonardo Cheng, Chi Woo Yoon, Peixiang He, Abdula Monther, Tianze Guo, Sarah Chittle, Yingxiao Wang
Summary: Cell-based immunotherapy, such as CAR-T cell immunotherapy, has revolutionized cancer treatment, but its application for solid tumors is limited by factors such as insufficient T cell tracking, tumor heterogeneity, inhibitory tumor microenvironment, and T cell exhaustion. Developing methods to overcome these limitations is critical for improving the effectiveness of immunotherapy for solid tumors.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Peripheral Vascular Disease
Sara Petrillo, F. De Giorgio, F. Bertino, F. Garello, V Bitonto, D. L. Longo, S. Mercurio, G. Ammirata, A. L. Allocco, V Fiorito, D. Chiabrando, F. Altruda, E. Terreno, P. Provero, L. Munaron, T. Genova, A. Novoa, A. R. Carlos, S. Cardoso, M. Mallo, M. P. Soares, E. Tolosano
Summary: The Feline Leukemia Virus Subgroup C Receptor 1a (FLVCR1a) is highly expressed in angiogenic endothelial cells (ECs) and has a critical role in developmental and pathological angiogenesis. Deficiency of FLVCR1a leads to abnormal vascular networks in multiple models, including defective vessel formation in zebrafish embryos, reduced retinal vasculature expansion and EC proliferation in mice, and disorganized and dysfunctional tumor blood vessels. These findings suggest FLVCR1a as a potential therapeutic target for diseases characterized by aberrant neovascularization.
Article
Biochemistry & Molecular Biology
Joon Seok Lee, Hyun Geun Cho, Jeong Yeop Ryu, Eun Jung Oh, Hyun Mi Kim, Suin Kwak, Seok-Jong Lee, Jongmin Lee, Sang Yub Lee, Seung Huh, Ji Yoon Kim, Ho Yun Chung
Summary: This study analyzed the effects of hypoxia on angiogenesis in arteriovenous malformation (AVM) patients and normal tissues. The results showed that AVM ECs exhibited higher angiogenesis capacity and expression of angiogenesis-related genes under hypoxic conditions. These findings provide a foundation for the development of AVM treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Lucio Diaz-Flores, Ricardo Gutierrez, Maria Pino Garcia, Miriam Gonzalez-Gomez, Lucio Diaz-Flores, Jose Luis Carrasco, Juan Francisco Madrid, Aixa Rodriguez Bello
Summary: Perivascular cells, including pericytes and CD34+ stromal cells/telocytes, play an important role in angiogenesis. The behavior and changes of these cells differ depending on the type of angiogenesis, involving various cell-to-cell interactions and signaling pathways. Perivascular cells and CD34+ stromal cells/telocytes have important functions in vessel development and tissue repair, while their involvement in intussusceptive angiogenesis is limited.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yang Yu, Xuechun Li, Yimei Li, Renyue Wei, Hai Li, Zhonghua Liu, Yu Zhang
Summary: This study establishes a stable iPSC-EC induction system and investigates the regulatory mechanism of endothelial differentiation. The derived piPSC-ECs form microvessel-like structures in vitro and promote blood vessel formation under pathological conditions. The activation of TGF-beta-Smad2/3 signaling promotes mesoderm formation and prevents endothelial differentiation in this model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Gianfranco Matrone, Bo Xia, Kaifu Chen, Martin A. Denvir, Andrew H. Baker, John P. Cooke
Summary: This study identified novel up-regulated genes during endothelial development and highlighted the crucial role of PRDM16 in regulating endothelial cell differentiation and angiogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Adrian Moure, Guillermo Vilanova, Hector Gomez
Summary: This study investigates the complex interactions between interstitial flow and the affinity for matrix binding of different chemokine isoforms using a computational model. The results demonstrate that changing the matrix affinity of the chemokine isoform can invert the effect of interstitial flow on angiogenesis. These findings provide insight into the intricate mechanisms of angiogenesis in the tumor microenvironment.
SCIENTIFIC REPORTS
(2022)
Review
Oncology
Wenzhe Duan, Shengkai Xia, Mengyi Tang, Manqing Lin, Wenwen Liu, Qi Wang
Summary: Interactions between endothelial cells (ECs) and tumor cells are crucial in brain tumor development. The heterogeneity of ECs and the use of new research models have received increasing attention in brain tumors. Targeting ECs is of particular importance due to the high degree of vascularization in brain tumors.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Biochemical Research Methods
Meenu Wadhwa, Hye-Na Kang, Hugo Hamel, Teeranart Jivapaisarnpong
Article
Hematology
C. Thelwell, P. Rigsby, M. Locke, S. Bevan, C. Longstaff
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2018)
Article
Medicine, Research & Experimental
Clive Metcalfe, Thomas Dougall, Chris Bird, Peter Rigsby, Marie-Emmanuelle Behr-Gross, Meenu Wadhwa, Melanie Morris, Lea-Ellen Hogie, Keith Mortimer, Haibin Wang, Lei Li, Bingjie Hao, Meng Li, Lan Wang, Jaana Vesterinen, Sylvie Jorajuria, Marie-Emmanuelle Behr-Gross, Jean-Claude Ourlin, Michael Tovey, Christophe Lallemand, Ulrike Herbrand, Kerstin Brack, Shubrata Khedkar, Prabhavathy Munagala, Ranjan Chakrabarti, Ezra Mulugeta, Charlotta Mark, Cornelius Fritsch, Ruzica Puljic, Chris Bird, Paula Dilger, Clive Metcalfe, Stuart Dunn, Guoping Wu, Todd Geders, Yong Suk Yang, Gye Mee Jang, Jia-Ming Yang, Yucai Peng, Hiroko Shibata, Masato Kiyoshi, Akiko Ishii-Watabe, Yeonjoo Hong, Omer Dushek, Marcus Bridge, John Nguyen, Chaomei Lin, Joseph Albanese, Ton Geurts, James Kessels, Raf Berghmans, Annick de Vries, Tom Lourens, Daniel Nagore, Susana Catarino, Melissa Snyder, Maria Willrich, Zehra Arkir, Ermis Parussini, Simon Daviere
Article
Hematology
Anthony R. Hubbard, Craig Thelwell, Peter Rigsby, P. Baker, J. Beavis, R. Gebauer, A. Riddell, S. Park, K. Jung, W. Kaar, S. Rosen, P. Bryngelhed, I Mackie, B. Young, A. Stroobants, A. Hunfeld, M. Kusch, G. Praefcke, S. Rosenkranz, A. Schroda, G. Moore, C. Dunsmore, R. Zadro, C. Lawrence, S. Bevan, D. Foulon, G. Teramura, A. Bowyer, S. Kitchen, M. Doyle, N. Binder, M. Ovanesov, Y. Liang, S. Surov, L. Parunov, J. Rigano, N. Gottschalk, E. Demaistre, F. Peyvandi, C. Novembrino, E. Jeanpierre, J. Grenet, C. Aime, M-B Maes, J. Sidelmann, N. Martineau, M. Grimaux, L. Johnson
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2019)
Letter
Cell Biology
Amanda E. I. Proudfoot, Meenu Wadhwa
JOURNAL OF LEUKOCYTE BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Shalom A. Gurjar, Jun X. Wheeler, Meenu Wadhwa, Robin Thorpe, Ian Kimber, Jeremy P. Derrick, Rebecca J. Dearman, Clive Metcalfe
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemical Research Methods
Meenu Wadhwa, Robin Thorpe
Editorial Material
Biochemical Research Methods
Meenu Wadhwa, Robin Thorpe
Article
Pharmacology & Pharmacy
Francesca Civoli, Aparna Kasinath, Xiao-Yan Cai, Meenu Wadhwa, Andrew Exley, Philip Oldfield, Safa Alvandkouhi, Gregor Schaffar, John Chappell, Ronald Bowsher, Viswanath Devanarayan, Joseph Marini, Shannon Rebarchak, Michael Anderson, Vera Koppenburg, Todd Lester
Article
Biochemical Research Methods
Meenu Wadhwa, Hye-Na Kang, Teeranart Jivapaisarnpong, Lucia Rizka Andalucia, Carolina Damas Rocha Zarate Blades, Mary Casas Levano, Weihong Chang, Jing Yin Chew, Mumbi Bernice Chilufya, Parichard Chirachanakul, Heeyoun (Gloria) Cho, Yi O. Cho, Kyung Min Choi, Sannie Chong, Hui Ming Chua, Ali Vasheghani Farahani, Mumun Gencoglu, Mariam Raouf Wefky Ghobrial, Prasunkumar Guha, Maria-Teresa Gutierrez Lugo, Sung Bae Ha, Suna Habahbeh, Hugo Hamel, Yeosun Hong, Aleksei Iarutkin, Hyunsook Jang, Ramalingam Jayachandran, Teeranart Jivapaisarnpong, Hye-Na Kang, Dong-young Kim, Gi Hyun Kim, Yunjeong Kim, Hyuk-Sang Kwon, Jacob Larsen, Aileen HyoJu Lee, Jiyoung Lee, Ksenia Medvedeva, Zuma Munkombwe, Ilung Oh, Jooyoung Park, Jooyoung Park, Desi Eka Putri, Jacqueline Rodgers, Sungmun Ryu, Maria Savkina, Thomas Schreitmueller, Oleh Semeniuk, Mira Seo, Yung In Shin, Jinho Shin, Shraddha Srivastava, HyeonHo Song, Suwon Song, Joao Tavares Neto, Meenu Wadhwa, Teruhide Yamaguchi, Hyun-Jun (David) Youn, Minae Yun
Review
Biochemical Research Methods
Hye-Na Kang, Robin Thorpe, Ivana Knezevic, Carolina Damas Rocha Zarate Blades, Mary Casas Levano, Jing Yin Chew, Mumbi Bernice Chilufya, Parichard Chirachanakul, Hui Ming Chua, Ali Vasheghani Farahani, Mariam Raouf Wefky Ghobrial, Suna Habahbeh, Hugo Hamel, Gi Hyun Kim, Violeta Perez Rodriguez, Desi Eka Putri, Jacqueline Rodgers, Maria Savkina, Oleh Semeniuk, Shraddha Srivastava, Meenu Wadhwa, Teruhide Yamaguchi
Letter
Biotechnology & Applied Microbiology
Sandra Prior, Clive Metcalfe, Simon E. Hufton, Meenu Wadhwa, Christian K. Schneider, Chris Burns
NATURE BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Jun X. Wheeler, Craig Thelwell, Peter Rigsby, Gail Whiting
Summary: In this study, a quantitative mass spectrometry method was established for measuring TAFI in human plasma. Traceability was achieved by using calibrators consisting of blank plasma spiked with a known amount of purified TAFI. This method enabled reliable and accurate measurement of TAFI concentration in plasma and demonstrated its applicability for assigning a common reference standard.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Hematology
E. O. Artemenko, S. I. Obydennyi, K. S. Troyanova, G. A. Novichkova, D. Y. Nechipurenko, M. A. Panteleev
Summary: In this study, we investigated the adhesive properties of circulating plasma-derived and platelet-derived microvesicles (MVs) and their potential role in thrombus growth. The results showed that both types of MVs weakly bound fibrinogen but did not stably adhere to immobilized fibrinogen. Moreover, the presence of these MVs did not significantly affect thrombus formation. Therefore, MVs present in the plasma of healthy individuals are unlikely to directly contribute to thrombus formation under arterial flow conditions.
THROMBOSIS RESEARCH
(2024)
Letter
Hematology
Giuseppe Lippi, Camilla Mattiuzzi, Emmanuel J. Favaloro
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Marium Malik, Maha Al-Ghafry, Abraham Haimed, Julia Su, Maribel Lema, Linda Shore-Lessersson, Suchitra S. Acharya
Summary: This study analyzed and evaluated leukemia-associated coagulopathy in newly diagnosed pediatric leukemia patients using CCTs and ROTEM. The results showed that fibrinogen and platelets played a significant role in causing coagulopathies in this population. This suggests the utility of using CCTs and ROTEM in the pediatric population to evaluate hemostatic function and guide blood product replacement.
THROMBOSIS RESEARCH
(2024)
Review
Hematology
Mohammad M. Elahi, Alexandra N. Witt, Edward L. G. Pryzdial, Paul B. McBeth
Summary: Thrombotic disease may be underdiagnosed in microgravity conditions, and the underlying factors are still poorly understood. A case of internal jugular vein thrombosis was recently diagnosed in a low-risk female astronaut, and six out of ten additional crew members showed risk factors for jugular venous flow. Observations in space and studies on Earth suggest that microgravity affects blood flow, coagulation, and vascular function.
THROMBOSIS RESEARCH
(2024)
Review
Hematology
Stian Ingemann-Molden, Christina Krogner Caspersen, Nanna Rolving, Anette Arbjerg Hojen, Frederikus A. Klok, Erik L. Grove, Barbara Cristina Brocki, Jane Andreasen
Summary: This study found that no single PROM covered all aspects considered important by PE patients. PROMs recommended in the ICHOM core set covered most aspects, but worrisome thoughts, hypervigilance around symptoms, and uncertainty of illness were not covered.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Haomin Yan, Tsutomu Sasaki, Yasufumi Gon, Kumiko Nishiyama, Hideaki Kanki, Hideki Mochizuki
Summary: The incidence of cancer-associated stroke has increased with the prolonged survival times of cancer patients. In this study, the researchers focused on the driver gene KRAS and found that KRAS mutations exacerbated neurological deficits after stroke and promoted tumor growth. They also identified the upregulation of pro-inflammatory responses and the promotion of STAT3 phosphorylation in mice with KRAS mutations. Additionally, the researchers found that colorectal cancer patients with stroke onset within 3 months after cancer diagnosis had a worse prognosis.
THROMBOSIS RESEARCH
(2024)
Letter
Hematology
Tomoki Togashi, Satomi Nagaya, Makiko Meguro-Horike, Haruto Matsumoto, Yuta Imai, Koichi Yamaguchi, Yoshinari Fujii, Haruka Moriya, Yuika Kikuchi, Ibuki Yasuda, Shin-ichi Horike, Eriko Morishita
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Marta Najarro, Winnifer Briceno, Carmen Rodriguez, Alfonso Muriel, Sara Gonzalez, Ana Castillo, Ignacio Jara, Parth Rali, Catalin Toma, Behnood Bikdeli, David Jimenez
Summary: The CPES score is a useful tool in identifying the risk of hemodynamic deterioration in normotensive patients with acute PE, with acceptable C-statistic, excellent specificity, and low positive predictive value.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Lei Liu, Huifang Xu, Jian Wang, Haiyan Wang, Saisai Ren, Qian Huang, Mingyan Zhang, Hui Zhou, Chunyan Yang, Lu Jia, Yu Huang, Hao Zhang, Yanling Tao, Ying Li, Yanan Min
Summary: This study investigated the gut microbiota metabolites and cytokines, chemokines, and growth factors in patients with ITP and found their association with treatment responsiveness. The findings suggest that imbalanced secretion of cyto(chemo)kins/growth factors and inadequate levels of TMAO are characteristic of patients with ITP.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Roxanne Mcknight, Mohammad Salameh, Lee Ann Jones, Greta Anton, Lindsay Carter
Summary: This study evaluated the safety of fixed-dose heparin titration boluses in underweight patients with VTE. The results showed that although underweight patients required more frequent holding of heparin, there was no significant increase in bleeding risk.
THROMBOSIS RESEARCH
(2024)