Article
Neurosciences
Tong Zhu, Yingao Li, Lilin Zhu, Jinyuan Xu, Zijun Feng, Hao Chen, Si Shi, Caiying Liu, Qingjian Ou, Furong Gao, Jieping Zhang, Caixia Jin, Jingying Xu, Jiao Li, Jingfa Zhang, Yanlong Bi, Guo-tong Xu, Juan Wang, Haibin Tian, Lixia Lu
Summary: This study identified GMFB as a sensitive and functional gliosis marker for diabetic retinal degeneration. Knockout of Gmfb improved visual function, attenuated gliosis, and reduced apoptosis in diabetic retinas. RNA sequencing analysis revealed the involvement of TGF-beta 3 in the protective effect of Gmfb knockout. The study provides a novel therapeutic strategy for treating retinal degeneration in patients with diabetic retinopathy.
Article
Cell & Tissue Engineering
Honghu Li, Qian Luo, Shuyang Cai, Ruxiu Tie, Ye Meng, Wei Shan, Yulin Xu, Xiangjun Zeng, Pengxu Qian, He Huang
Summary: Gmfg plays a crucial role in hematopoietic stem and progenitor cell (HSPC) development. It is regulated by blood flow and modulates HSPC maintenance through YAP and Notch signaling pathways.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Immunology
Ryota Yamagishi, Fumitaka Kamachi, Masaru Nakamura, Shota Yamazaki, Tomonori Kamiya, Masaki Takasugi, Yi Cheng, Yoshiki Nonaka, Yoshimi Yukawa-Muto, Le Thi Thanh Thuy, Yohsuke Harada, Tatsuya Arai, Tze Mun Loo, Shin Yoshimoto, Tatsuya Ando, Masahiro Nakajima, Hayao Taguchi, Takamasa Ishikawa, Hisaya Akiba, Sachiko Miyake, Masato Kubo, Yoichiro Iwakura, Shinji Fukuda, Wei-Yu Chen, Norifumi Kawada, Alexander Rudensky, Susumu Nakae, Eiji Hara, Naoko Ohtani
Summary: In this study, the release mechanism of senescence-associated secretory phenotype (SASP) factors from senescent hepatic stellate cells (HSCs) was identified in a mouse model of obesity-induced hepatocellular carcinoma (HCC). IL-33 release from HSCs promoted HCC development via the activation of ST2-positive T-reg cells in the liver tumor microenvironment. These findings highlight the therapeutic potential of inhibitors of gasdermin D-mediated pore formation for HCC treatment.
SCIENCE IMMUNOLOGY
(2022)
Article
Immunology
Peter W. West, Rajia Bahri, Karen M. Garcia-Rodriguez, Georgia Sweetland, Georgia Wileman, Rajesh Shah, Angeles Montero, Laura Rapley, Silvia Bulfone-Paus
Summary: Aberrant mast cell responses and complement activation both contribute to allergic diseases. IL-33 plays a critical role in regulating mast cell responses to complement anaphylatoxins, enhancing mast cell reactivity to C3a and C5a. This cross-regulation may aggravate Th2 immune responses and targeting anti-IL33 therapeutically could provide a rationale in allergic diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Zuleyha Doganyigit, Kaan Erbakan, Enes Akyuz, Ayse Kristina Polat, Alina Arulsamy, Mohd Farooq Shaikh
Summary: Traumatic brain injury (TBI) is a severe neurological disorder with long-term risks of various neurological and neurodegenerative diseases. Neuroinflammatory mediators, particularly inflammatory cytokines, play a crucial role in the functional and pathological outcomes of TBI. Early identification and treatment of these mediators can contribute to the development of more effective treatment options for TBI.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Jingjing Liu, Yongsheng Hou, Lili Lin, Nannan Yu, Yanyan Zhang
Summary: The study found that miR-5195-3p plays an important role in high glucose-induced injury in human retinal pigment epithelial cells, and targeting GMFB may become a potential therapeutic target for diabetic retinopathy.
Article
Biochemistry & Molecular Biology
Caiying Liu, Wan Sun, Tong Zhu, Si Shi, Jieping Zhang, Juan Wang, Furong Gao, Qingjian Ou, Caixia Jin, Jiao Li, Jing-Ying Xu, Jingfa Zhang, Haibin Tian, Guo-Tong Xu, Lixia Lu
Summary: Diabetic retinopathy (DR) is a major cause of blindness, and timely prevention and treatment are crucial. This study found that the neurodegenerative factor GMFB is upregulated in the early stages of diabetes and plays a role in the pathogenesis of the disease. In a high glucose environment, GMFB secretion in the vitreous affects lysosome function, autophagy, and ferroptosis, leading to abnormalities in the degradation process and the accumulation of lethal lipid species in the retinal pigment epithelial cells. Targeting GMFB and related pathways could be a new therapeutic strategy for DR.
Review
Biotechnology & Applied Microbiology
Ghalya H. Banafea, Sherin Bakhashab, Huda F. Alshaibi, Peter Natesan Pushparaj, Mahmood Rasool
Summary: Mast cells play a crucial role in inflammatory diseases of the airway tract, releasing various mediators that contribute to immune cell accumulation and tissue remodeling.
Article
Immunology
Alison McCallion, Yasmin Nasirzadeh, Harshavardhan Lingegowda, Jessica E. Miller, Kasra Khalaj, SooHyun Ahn, Stephany P. Monsanto, Mallikarjun Bidarimath, Danielle J. Sisnett, Andrew W. Craig, Steven L. Young, Bruce A. Lessey, Madhuri Koti, Chandrakant Tayade
Summary: This study found that mast cell numbers significantly increase in endometriotic lesions, and the microenvironment within these lesions promotes the recruitment and differentiation of mast cells. There is cross-talk between mast cells and endocrine and immune factors, which may contribute to the pathophysiology of endometriosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Johanna Seifert, Claudia Kuechler, Sebastian Drube
Summary: Mast cells (MCs) are sentinel cells that play an important role in the immune system's first line of defense. They sense the danger-associated molecular patterns (DAMPs) IL-33 and ATP, which are released upon cell damage, and respond to pathogens and allergens. The stem cell factor (SCF) maintains the functionality of MCs by regulating their cytokine production.
Article
Cell Biology
Qiong Wu, Zongwen Liang, Jing Jiang, Xiaoming Feng, Jinming Liu, Zongfeng Zhang, Honglin Wang, Ning Wang, Yanling Gou, Zhi Li, Yingying Cao
Summary: IL-33 secreted from macrophages regulates ferroptosis in eESCs through the P38/JNK/ATF3 pathway, ultimately protecting eESCs from iron-induced cell death.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Kanan Bando, Yukinori Tanaka, Saka Winias, Shunji Sugawara, Itaru Mizoguchi, Yasuo Endo
Summary: IL-33 is released upon cell injury and induces histamine release and local inflammation. IL-33 directly and indirectly induces histamine release through histidine decarboxylase (HDC), and the newly formed histamine contributes to the negative regulation of IL-33-induced eosinophilia via H4 receptors.
INFLAMMATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Isabel Peters, Sylvia Mueller, Claudia Kuechler, Ute Jaeger, Sebastian Drube
Summary: IL-33 induces the activation of signaling pathways in MCs, leading to the production of pro-inflammatory cytokines and allergic reactions. HSP90, which is upregulated during allergic reactions, plays a role in stabilizing cytokine mRNA and facilitating cytokine production. Therefore, targeting HSP90 could be a potential therapeutic intervention for blocking IL-33-mediated inflammatory reactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
America Vera-Montecinos, Jordi Galiano-Landeira, Monica Roldan, Francisco Vidal-Domenech, Enrique Claro, Belen Ramos
Summary: METTL7A is mainly located in the endoplasmic reticulum and lipid droplets, with limited cellular expression in the brain. Reduced protein levels of METTL7A have been found in schizophrenia. Our study shows that METTL7A is highly expressed in Bergmann glia and has contacts with Purkinje neurons. The localization of METTL7A may play a role in maintaining cerebellar homeostasis and modulating cerebellar circuits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Deniz Ragipoglu, Jasmin Buelow, Kristin Hauff, Martin Voss, Melanie Haffner-Luntzer, Anne Dudeck, Anita Ignatius, Verena Fischer
Summary: Mast cells play an important role in inflammation induced by trauma, contributing to compromised bone healing.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Critical Care Medicine
Mohammad Ejaz Ahmed, Govindhasamy Pushpavathi Selvakumar, Duraisamy Kempuraj, Sudhanshu P. Raikwar, Ramasamy Thangavel, Kieran Bazley, Kristopher Wu, Osaid Khan, Klaudia Kukulka, Bret Bussinger, Iuliia Dubova, Smita Zaheer, Raghav Govindarajan, Shankar Iyer, Casey Burton, Donald James, Asgar Zaheer
JOURNAL OF NEUROTRAUMA
(2020)
Article
Cell Biology
Sudhanshu P. Raikwar, Ramasamy Thangavel, Mohammad Ejaz Ahmed, Govindhasamy Pushpavathi Selvakumar, Duraisamy Kempuraj, Kristopher Wu, Osaid Khan, Kieran Bazley, Bret Bussinger, Klaudia Kukulka, Smita Zaheer, Shankar S. Iyer, Raghav Govindarajan, Casey Burton, Donald James, Asgar Zaheer
Summary: Understanding the activation of NF kappa B signaling post-TBI is crucial for neuroinflammation and neurodegeneration. Real-time noninvasive bioluminescence molecular imaging allows rapid and precise monitoring of TBI-induced NF kappa B signaling activation.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2021)
Article
Neurosciences
Mohammad Ejaz Ahmed, Govindhasamy Pushpavathi Selvakumar, Ramasamy Thangavel, Duraisamy Kempuraj, Sudhanshu P. Raikwar, Smita Zaheer, Shankar Iyer, Asgar Zaheer
Summary: The study demonstrates that immune checkpoint blockade of GMF function with anti-GMF antibody can effectively reduce neuroinflammation and attenuate amyloid pathology in the cortex and hippocampal CA1 region of 5XFAD mouse brain. It also suggests that pharmacological immune neutralization of GMF could be a promising neuroprotective strategy for targeting neuroinflammation and neurodegeneration in Alzheimer's disease.
JOURNAL OF NEUROIMMUNE PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Duraisamy Kempuraj, Ramasamy Thangavel, Deepak D. Kempuraj, Mohammad Ejaz Ahmed, Govindhasamy Pushpavathi Selvakumar, Sudhanshu P. Raikwar, Smita A. Zaheer, Shankar S. Iyer, Raghav Govindarajan, Premkumar Nattanmai Chandrasekaran, Asgar Zaheer
Summary: Neuroinflammation caused by neurotrauma and neurodegenerative diseases can lead to cognitive decline and chronic inflammation. Luteolin, a natural polyphenol found in plants, shows neuroprotective effects by suppressing immune cell activation and inflammatory mediators, reducing neuroinflammation and severity of diseases like Alzheimer's, Parkinson's, multiple sclerosis, and TBI.
Article
Neurosciences
Duraisamy Kempuraj, Mohammad Ejaz Ahmed, Govindhasamy Pushpavathi Selvakumar, Ramasamy Thangavel, Sudhanshu P. Raikwar, Smita A. Zaheer, Shankar S. Iyer, Raghav Govindarajan, Premkumar Nattanmai Chandrasekaran, Casey Burton, Donald James, Asgar Zaheer
Summary: Acute traumatic brain injury leads to neuroinflammation and neurovascular alterations, including blood-brain barrier disruption, which can be effectively inhibited by the administration of the mast cell stabilizer drug cromolyn.
NEUROTOXICITY RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Ruchira M. Jha, Sudhanshu P. Raikwar, Sandra Mihaljevic, Amanda M. Casabella, Joshua S. Catapano, Anupama Rani, Shashvat Desai, Volodymyr Gerzanich, J. Marc Simard
Summary: Cerebral edema is a significant factor in brain injury mortality and disability. Current treatments are limited, prompting the development of targeted therapies based on a growing understanding of the molecular mechanisms. Promising molecular targets, including ion and water channels and proteins/lipids involved in edema signaling pathways, are being investigated for their potential to reduce cerebral edema and improve clinical outcomes.
EXPERT OPINION ON THERAPEUTIC TARGETS
(2021)
Review
Biochemistry & Molecular Biology
Ruchira M. Jha, Anupama Rani, Shashvat M. Desai, Sudhanshu Raikwar, Sandra Mihaljevic, Amanda Munoz-Casabella, Patrick M. Kochanek, Joshua Catapano, Ethan Winkler, Giuseppe Citerio, J. Claude Hemphill, W. Taylor Kimberly, Raj Narayan, Juan Sahuquillo, Kevin N. Sheth, J. Marc Simard
Summary: SUR1, a member of the ABC protein superfamily, is a key mediator of CNS cellular swelling through the TRPM4 channel. Inhibition of SUR1-TRPM4 pathway has shown promising results in reducing cerebral edema and hemorrhage across a range of CNS diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Suneel Gupta, K. Michael Fink, Duraisamy Kempuraj, R. Nishant Sinha, M. Lynn Martin, M. Landon Keele, R. Prashant Sinha, A. Elizabeth Giuliano, P. Nathan Hesemann, P. Sudhanshu Raikwar, S. Shyam Chaurasia, R. Rajiv Mohan
Summary: This study evaluated the potential of AAV5-mediated Id3 gene therapy to treat corneal scarring. The results showed that AAV5-Id3 gene therapy significantly inhibited corneal fibrosis/haze and reduced the expression levels of related proteins and genes. This anti-fibrotic activity was achieved by reducing the formation of myofibroblasts.
Correction
Biotechnology & Applied Microbiology
Suneel Gupta, Michael K. Fink, Duraisamy Kempuraj, Nishant R. Sinha, Lynn M. Martin, Landon M. Keele, Prashant R. Sinha, Elizabeth A. Giuliano, Nathan P. Hesemann, Sudhanshu P. Raikwar, Shyam S. Chaurasia, Rajiv R. Mohan
Review
Biochemistry & Molecular Biology
Anant Naik, Olufunmilola Adeleye, Stefan W. Koester, Ethan A. Winkler, Joelle N. Hartke, Katherine Karahalios, Sandra Mihaljevic, Anupama Rani, Sudhanshu Raikwar, Jarrod D. Rulney, Shashvat M. Desai, Lea Scherschinski, Andrew F. Ducruet, Felipe C. Albuquerque, Michael T. Lawton, Joshua S. Catapano, Ashutosh P. Jadhav, Ruchira M. Jha
Summary: Despite the high incidence and burden of stroke, biological biomarkers are not routinely used for diagnosing, determining progression, or predicting outcomes of acute ischemic stroke (AIS). However, cerebrospinal fluid (CSF) shows potential as a valuable source for biomarker development due to its direct interface with neural tissue. This systematic review identified 21 biomarkers and other multiomic proteomic markers, including S100B, inflammatory markers, and free fatty acids, that have been studied frequently. CSF has been shown to be an effective medium for acquiring biomarkers for AIS, although more large-scale trials studying the efficacy of biomarkers in altering clinical management are still needed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Critical Care Medicine
Benjamin Zusman, Yijen Wu, J. Marc Simard, Vincent Vagni, Keri Janesko-Feldman, Anupama Rani, Sudhanshu Raikwar, Shashvat Desai, Patrick Kochanek, Ruchira Jha
JOURNAL OF NEUROTRAUMA
(2021)