Article
Chemistry, Multidisciplinary
Xiaoyu Xu, Chang Liu, Shengyi Wang, Ermei Makila, Jiali Wang, Oliver Koivisto, Junnian Zhou, Jessica M. Rosenholm, Yilai Shu, Hongbo Zhang
Summary: In this study, a microfluidic-assisted metal-organic framework (MOF) based biomineralization strategy was developed for the efficient and remote control delivery of CRISPR/Cas9 RNP gene editing. The strategy involved growing thermo-responsive EuMOFs onto a photothermal template Prussian blue (PB) and loading RNP during MOFs crystallization in microfluidic channels. The resulting nanocarrier showed high loading efficiency, protection of RNP, and NIR-stimulated release capacity. Upon laser exposure, the nanocarrier demonstrated effective endosomal escape and precise gene knockout. The gene-editing activity could be programmed by tuning exposure times, providing a more controllable editing modality compared to the control group without laser irradiation.
Article
Chemistry, Physical
Mengna Zhang, Shujing Zhang, Kun Zhang, Zongyuan Zhu, Yalei Miao, Yudian Qiu, Panke Zhang, Xubo Zhao
Summary: A novel strategy was proposed to enhance the functionality of polymer-based nanostructures through the design of medicated hydrophobic chains, improving the efficiency of drug delivery systems and the suppression against cancer cells.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Nanoscience & Nanotechnology
Pan He, Bingtong Tang, Yusheng Li, Yu Zhang, Xinming Liu, Xin Guo, Dong Wang, Peng She, Chunsheng Xiao
Summary: This study demonstrates the feasibility of a novel oxidation-sensitive nanocarrier for curcumin in anti-inflammatory therapy, with significant potential shown in both in vitro and in vivo anti-inflammatory effects.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Chemistry, Physical
Amir Pourabed, Tayyaba Younas, Chang Liu, Bhuvana K. Shanbhag, Lizhong He, Tuncay Alan
Summary: This study successfully tuned the size of protein nanoparticles through a mechanical route by modifying the mixing time and strength, while keeping the chemical parameters constant. Using an acoustically actuated micro-mixer allowed for the assembly of protein particles with tunable sizes, leading to smaller particles with a more uniform size distribution than traditional batch processes.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Biophysics
Jiagen Li, Yaqi Li, Zhanqiong Zhong, Xiaohong Fu, Zhonghui Li
Summary: Chitosan-coated PMAA hollow spheres with high drug loading capacity and low drug leakage were successfully prepared through a novel self-assembly process. The drug release behavior of the spheres was pH and GSH responsive, allowing for controlled drug delivery. This study demonstrates the effectiveness of the one-pot two-step fabrication process in producing high-performance drug delivery systems.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Rujie Sun, Xin Song, Kun Zhou, Yuyang Zuo, Richard Wang, Omar Rifaie-Graham, David J. Peeler, Ruoxiao Xie, Yixuan Leng, Hongya Geng, Giulia Brachi, Yun Ma, Yutong Liu, Lorna Barron, Molly M. Stevens
Summary: This study presents a versatile platform for fabricating fillable microrobots using microfluidic loading and dip sealing (MLDS), which allows for encapsulating different types of cargoes within hollow microrobots and protecting the cargo integrity. The technique is supported by high-resolution 3D printing and an integrated microfluidic loading system, enabling a highly precise loading process and improved cargo loading capacity. The MLDS platform offers new opportunities for targeted drug delivery, environmental sensing, and chemically powered micromotor applications.
ADVANCED MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Federica Sommonte, Ilaria Arduino, Rosa Maria Iacobazzi, Mattia Tiboni, Federico Catalano, Roberto Marotta, Martina Di Francesco, Luca Casettari, Paolo Decuzzi, Angela Assunta Lopedota, Nunzio Denora
Summary: After 20 years of research, nanoscale delivery systems for biologicals in nanomedicine have become clinically relevant. Microfluidic-based production of solid lipid nanoparticles (SLNs) is shown to be efficient in encapsulating and delivering lysozyme (LZ), with smaller size and higher encapsulation efficacy compared to conventional methods. This study demonstrates the potential of microfluidic production of SLNs for encapsulation and delivery of complex biological molecules.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Huaiyou Chen, Ali Emre Celik, Angela Mutschler, Antoine Combes, Anne Runser, Andrey S. Klymchenko, Sebastien Lecommandoux, Christophe A. Serra, Andreas Reisch
Summary: Nanoprecipitation is a facile and efficient method for assembling loaded polymer nanoparticles in bioimaging and targeted drug delivery applications. Microfluidics is an attractive technique for reproducible and scalable synthesis. The study compares different types of microfluidic mixers in terms of nanoparticle formation and properties, and provides guidance for choosing the optimal microfluidic setup for producing nanomaterials for biomedical applications.
Article
Polymer Science
Anna Mielanczyk, Maria Kupczak, Olesya Klymenko, Lukasz Mielanczyk, Sebastian Arabasz, Krystian Madej, Dorota Neugebauer
Summary: Well-defined miktoarm star-shaped polymers and linear block copolymers have been successfully synthesized based on a heterofunctional glucose derivative initiator, N,N'-dimethylaminoethyl methacrylate (DMAEMA), using a combination of atom transfer radical polymerization (ATRP) and ring-opening polymerization (ROP) techniques followed by click reactions. The presence of salts in polymer solutions affects the phase transformation and aggregate sizes, with the critical aggregation concentration (CAC) decreasing with decreasing DMAEMA unit content. TEM and AFM analysis reveal the microstructure changes in samples with different compositions of polyester arms.
Article
Chemistry, Multidisciplinary
Yi Jin, Mhd Anas Tomeh, Peng Zhang, Mingzhu Su, Xiubo Zhao, Zhiqiang Cai
Summary: This study utilized a newly developed microfluidic swirl mixer to fabricate temperature-sensitive liposomes (TSL) for the treatment of breast cancer through near-infrared stimulation. The results demonstrated that AP-3/ICG@TSL significantly inhibited the growth of breast cancer both in vitro and in vivo without causing damage to other organs. These findings suggest that the AP-3/ICG@TSL manufactured using the microfluidic swirl mixer is a promising drug delivery system for breast cancer therapy.
Article
Polymer Science
Eva Mueller, Sebastian Himbert, Madeline J. Simpson, Markus Bleuel, Maikel C. Rheinstadter, Todd Hoare
Summary: The study demonstrates a novel approach for preparing degradable temperature/pH dual-responsive microgels with potential applications in drug delivery. These microgels exhibit dual-responsive swelling properties and degradability, making them suitable for drug delivery applications requiring dual pH/temperature-specific delivery at infection sites or cancer.
Article
Nanoscience & Nanotechnology
Guolong Gu, Chen Chen, Shichao Zhang, Bo Yin, Jianxin Wang
Summary: A novel strategy of delivering nitric oxide (NO) for cancer chemotherapy was proposed using prodrug dimer self-assembly nanoparticles of NO donors, which significantly improved drug loading and delivery efficiencies, ultimately inducing tumor cell apoptosis. This approach opened up a new avenue for cancer chemotherapy and laid a theoretical basis for clinical translation of NO-based gas chemotherapy.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Multidisciplinary Sciences
Jian-Shuang Guo, Juan-Juan Li, Ze-Han Wang, Yang Liu, Yu-Xin Yue, Hua-Bin Li, Xiu-He Zhao, Yuan-Jun Sun, Ya-Hui Ding, Fei Ding, Dong-Sheng Guo, Liang Wang, Yue Chen
Summary: This study presents a supramolecular dual hypoxia-responsive BE-based complex that achieves efficient drug delivery within tumors, demonstrating promising results in inhibiting tumor growth in pancreatic cancer cells xenograft murine model.
NATURE COMMUNICATIONS
(2023)
Article
Robotics
Hongyue Zhang, Zesheng Li, Changyong Gao, Xinjian Fan, Yuxin Pang, Tianlong Li, Zhiguang Wu, Hui Xie, Qiang He
Summary: Neutrophil-based microrobots, constructed by phagocytosis of drug-loaded magnetic nanogels enveloped in Escherichia coli membrane, exhibit controllable intravascular movement and can autonomously aggregate in the brain by positive chemotactic motion along the gradient of inflammatory factors. These dual-responsive neutrobots show promise for targeted drug delivery to inhibit tumor cell proliferation compared to traditional drug injection, leveraging the biological characteristics of natural neutrophils that artificial microrobots cannot replicate.
Article
Chemistry, Multidisciplinary
Hongxu Liu, Chiara Lionello, Jenna Westley, Annalisa Cardellini, Uyen Huynh, Giovanni M. Pavan, S. Thayumanavan
Summary: Understanding the structural factors that control the dynamics of supramolecular assemblies and modulating the dynamics transition point by subtle changes in hydrophobic patches on the hydrophilic face help in the rational design of enzyme-responsive assemblies with tunable temperature responsive profiles.
Article
Chemistry, Multidisciplinary
Fan Yang, Artiom Skripka, Antonio Benayas, Xianke Dong, Sung Hwa Hong, Fuqiang Ren, Jung Kwon Oh, Xinyu Liu, Fiorenzo Vetrone, Dongling Ma
ADVANCED FUNCTIONAL MATERIALS
(2018)
Article
Chemistry, Multidisciplinary
Fan Yang, Artiom Skripka, Maryam Sadat Tabatabaei, Sung Hwa Hong, Fuqiang Ren, Antonio Benayas, Jung Kwon Oh, Sylvain Martel, Xinyu Liu, Fiorenzo Vetrone, Dongling Ma
Article
Chemistry, Multidisciplinary
Na Re Ko, Sung Hwa Hong, Md Nafiujjaman, Seong Yeong An, Vishnu Revuri, Sang Ju Lee, Il Keun Kwon, Yong-Kyu Lee, Seung Jun Oh
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Shell Ip, Christina M. MacLaughlin, Michelle Joseph, Nisa Mullaithilaga, Guisheng Yang, Chen Wang, Gilbert C. Walker
Article
Chemistry, Physical
Fan Yang, Artiom Skripka, Maryam Sadat Tabatabaei, Sung Hwa Hong, Fuqiang Ren, Yue Huang, Jung Kwon Oh, Sylvain Martel, Xinyu Liu, Fiorenzo Vetrone, Dongling Ma
CHEMISTRY OF MATERIALS
(2019)
Article
Medicine, Research & Experimental
Nobuhito Hamano, Roland Bottger, Suen Ern Lee, Yang Yang, Jayesh A. Kulkarni, Shell Ip, Pieter R. Cullis, Shyh-Dar Li
MOLECULAR PHARMACEUTICS
(2019)
Article
Chemistry, Multidisciplinary
Na Re Ko, Se Young Van, Sung Hwa Hong, Seog-Young Kim, Miran Kim, Jae Seo Lee, Sang Ju Lee, Yong-kyu Lee, Il Keun Kwon, Seung Jun Oh
Review
Immunology
Anna K. Blakney, Shell Ip, Andrew J. Geall
Summary: This review explores the four major pillars required for design and development of an saRNA vaccine, discussing antigen design, vector design, non-viral delivery systems, and manufacturing. It reports on major innovations, preclinical and clinical data from the last five years, and provides insights into future prospects.
Article
Medicine, Research & Experimental
Cameron Webb, Shell Ip, Nuthan Bathula, Petya Popova, Shekinah K. Soriano, Han Han Ly, Burcu Eryilmaz, Viet Anh Nguyen Huu, Richard Broadhead, Martin Rabel, Ian Villamagna, Suraj Abraham, Vahid Raeesi, Anitha Thomas, Samuel Clarke, Euan C. Ramsay, Yvonne Perrie, Anna K. Blakney
Summary: The COVID-19 pandemic has led to the rapid development of mRNA vaccines, thanks to the modular nature of mRNA sequences and lipid nanoparticle (LNP) technology. The success of mRNA vaccines opens up possibilities for future RNA medicines, but there is already a strain on manufacturing capacity, which will be exacerbated by the development of other mRNA drugs in the near future.
MOLECULAR PHARMACEUTICS
(2022)
Article
Materials Science, Multidisciplinary
HaoTian Harvey Shi, Sung Hwa Hong, Hani E. Naguib
Summary: A multi-functional photo-reduced graphene oxide fabric has been developed using a laser direct-write technique. This fabric is created without the need for binders or supporting materials and exhibits tuneable microscale porosity. The technique offers potential applications in Joule heating, temperature sensing, humidity sensing, and other fields.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Chemistry, Physical
Sung Hwa Hong, HaoTian Harvey Shi, Hani E. Naguib
Summary: Polypyrrole-based electrodes with desired pseudocapacitive storage capabilities have been widely studied, but their utilization in flexible supercapacitor electrodes has been limited. In this study, a surface-modified thermoplastic polyurethane (TPU) was used as a flexible substrate and coated with carbon nanotubes (CNTs) to improve conductivity and interface with PPy nanofoam (PPyNF) structures. The resulting TPU/CNT/PPyNF electrodes demonstrated good flexibility and charge storage capability.
ACS APPLIED ENERGY MATERIALS
(2022)
Article
Engineering, Environmental
Sung Hwa Hong, Tianhao Chen, GuoRui Wang, Simon M. Popovic, Tobin Filleter, Hani E. Naguib
Summary: A self-healing polymer network based on poly (ODMS-co-MAA) is reported for soft piezoresistive pressure sensors. The incorporation of dynamic Fe(III)-carboxylate linkages enables the sensor to recover functionality and electrical resistance after mechanical damage. The microdome structure of the sensor exhibits superior pressure sensitivity and stability compared to planar features.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Stephanie N. N. Iwasa, HaoTian H. H. Shi, Sung Hwa Hong, Tianhao Chen, Melissa Marquez-Chin, Christian Iorio-Morin, Suneil K. K. Kalia, Milos R. R. Popovic, Hani E. E. Naguib, Cindi M. M. Morshead
Article
Polymer Science
Arman Moini Jazani, Newsha Arezi, Chaitra Shetty, Sung Hwa Hong, Haowen Li, Xiangtao Wang, Jung Kwon Oh