Current Status and Future Perspectives on MRNA Drug Manufacturing

BNT162b vaccines protect rhesus macaques from SARS-CoV-2

(2021) Annette B. Vogel et al.   NATURE

SARS-CoV-2 vaccines strategies: a comprehensive review of phase 3 candidates

(2021) Nikolaos C. Kyriakidis et al.   npj Vaccines

mRNA vaccines manufacturing: Challenges and bottlenecks

(2021) Sara Sousa Rosa et al.   VACCINE

Taiwan and the foundry model

(2021) Mark Liu   Nature Electronics

Scalable mRNA and siRNA Lipid Nanoparticle Production Using a Parallelized Microfluidic Device

(2021) Sarah J. Shepherd et al.   NANO LETTERS

A Combinatorial Library of Lipid Nanoparticles for RNA Delivery to Leukocytes

(2020) Srinivas Ramishetti et al.   ADVANCED MATERIALS

Using microfluidics for scalable manufacturing of nanomedicines from bench to GMP: A case study using protein-loaded liposomes

(2020) Cameron Webb et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

SARS-CoV-2 mRNA vaccine design enabled by prototype pathogen preparedness

(2020) Kizzmekia S. Corbett et al.   NATURE

Phase 1/2 study of COVID-19 RNA vaccine BNT162b1 in adults

(2020) Mark J. Mulligan et al.   NATURE

Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates

(2020) Kizzmekia S. Corbett et al.   NEW ENGLAND JOURNAL OF MEDICINE

An mRNA Vaccine against SARS-CoV-2 — Preliminary Report

(2020) Lisa A. Jackson et al.   NEW ENGLAND JOURNAL OF MEDICINE

Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice

(2020) Paul F. McKay et al.   Nature Communications

Delivery of self-amplifying mRNA vaccines by cationic lipid nanoparticles: The impact of cationic lipid selection

(2020) Gustavo Lou et al.   JOURNAL OF CONTROLLED RELEASE

COVID-19 vaccine BNT162b1 elicits human antibody and TH1 T cell responses

(2020) Ugur Sahin et al.   NATURE

Safety and Immunogenicity of Two RNA-Based Covid-19 Vaccine Candidates

(2020) Edward E. Walsh et al.   NEW ENGLAND JOURNAL OF MEDICINE

Targeted Inhibition of the NUP98-NSD1 Fusion Oncogene in Acute Myeloid Leukemia

(2020) Sagarajit Mohanty et al.   Cancers

Manufacturing Considerations for the Development of Lipid Nanoparticles Using Microfluidics

(2020) Carla B. Roces et al.   Pharmaceutics

Systemic mRNA Therapy for the Treatment of Fabry Disease: Preclinical Studies in Wild-Type Mice, Fabry Mouse Model, and Wild-Type Non-human Primates

(2019) Xuling Zhu et al.   AMERICAN JOURNAL OF HUMAN GENETICS

PTCD1 is required for mitochondrial oxidative-phosphorylation: possible genetic association with Alzheimer's disease

(2019) Daniel Fleck et al.   JOURNAL OF NEUROSCIENCE

Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts

(2019) Jacob L. Litke et al.   NATURE BIOTECHNOLOGY

Optimization of Lipid Nanoparticles for Intramuscular Administration of mRNA Vaccines

(2019) Kimberly J. Hassett et al.   Molecular Therapy-Nucleic Acids

Self-Amplifying RNA Vaccines Give Equivalent Protection against Influenza to mRNA Vaccines but at Much Lower Doses

(2018) Annette B. Vogel et al.   MOLECULAR THERAPY

A Novel Amino Lipid Series for mRNA Delivery: Improved Endosomal Escape and Sustained Pharmacology and Safety in Non-human Primates

(2018) Staci Sabnis et al.   MOLECULAR THERAPY

A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing

(2018) Jonathan D. Finn et al.   Cell Reports

Systemic messenger RNA as an etiological treatment for acute intermittent porphyria

(2018) Lei Jiang et al.   NATURE MEDICINE

Rapid and scale-independent microfluidic manufacture of liposomes entrapping protein incorporating in-line purification and at-line size monitoring

(2018) Neil Forbes et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Polymerase Chain Transcription: Exponential Synthesis of RNA and Modified RNA

(2017) Tingjian Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Efficient Targeting and Activation of Antigen-Presenting Cells In Vivo after Modified mRNA Vaccine Administration in Rhesus Macaques

(2017) Frank Liang et al.   MOLECULAR THERAPY

Lipid Nanoparticle Systems for Enabling Gene Therapies

(2017) Pieter R. Cullis et al.   MOLECULAR THERAPY

Preclinical and Clinical Demonstration of Immunogenicity by mRNA Vaccines against H10N8 and H7N9 Influenza Viruses

(2017) Kapil Bahl et al.   MOLECULAR THERAPY

Boosting Intracellular Delivery of Lipid Nanoparticle-Encapsulated mRNA

(2017) Siddharth Patel et al.   NANO LETTERS

Systemic delivery of factor IX messenger RNA for protein replacement therapy

(2017) Suvasini Ramaswamy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Safety Evaluation of Lipid Nanoparticle–Formulated Modified mRNA in the Sprague-Dawley Rat and Cynomolgus Monkey

(2017) Maja Sedic et al.   VETERINARY PATHOLOGY

Systemic Messenger RNA Therapy as a Treatment for Methylmalonic Acidemia

(2017) Ding An et al.   Cell Reports

Unmodified mRNA in LNPs constitutes a competitive technology for prophylactic vaccines

(2017) Johannes Lutz et al.   npj Vaccines

Non-Viral CRISPR/Cas Gene Editing In Vitro and In Vivo Enabled by Synthetic Nanoparticle Co-Delivery of Cas9 mRNA and sgRNA

(2016) Jason B. Miller et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Immune modulatory nanoparticle therapeutics for intracerebral glioma

(2016) Nasser K. Yaghi et al.   NEURO-ONCOLOGY

Intrathecal delivery of frataxin mRNA encapsulated in lipid nanoparticles to dorsal root ganglia as a potential therapeutic for Friedreich’s ataxia

(2016) Joseph F. Nabhan et al.   Scientific Reports

Expression kinetics of nucleoside-modified mRNA delivered in lipid nanoparticles to mice by various routes

(2015) Norbert Pardi et al.   JOURNAL OF CONTROLLED RELEASE

A Cationic Nanoemulsion for the Delivery of Next-generation RNA Vaccines

(2014) Luis A Brito et al.   MOLECULAR THERAPY

Identification of a T7 RNA polymerase variant that permits the enzymatic synthesis of fully 2′-O-methyl-modified RNA

(2013) Jenny Ibach et al.   JOURNAL OF BIOTECHNOLOGY

Production of pure and functional RNA for in vitro reconstitution experiments

(2013) Franziska Theresia Edelmann et al.   METHODS

Biodegradable Lipids Enabling Rapidly Eliminated Lipid Nanoparticles for Systemic Delivery of RNAi Therapeutics

(2013) Martin A Maier et al.   MOLECULAR THERAPY

A short adaptive path from DNA to RNA polymerases

(2012) C. Cozens et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nonviral delivery of self-amplifying RNA vaccines

(2012) A. J. Geall et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Systemic RNAi-mediated Gene Silencing in Nonhuman Primate and Rodent Myeloid Cells

(2012) Tatiana I Novobrantseva et al.   Molecular Therapy-Nucleic Acids

Microfluidic Synthesis of Highly Potent Limit-size Lipid Nanoparticles for In Vivo Delivery of siRNA

(2012) Nathan M Belliveau et al.   Molecular Therapy-Nucleic Acids

A brief review of DNA and RNA chemical synthesis: Figure 1

(2011) Marvin H. Caruthers   BIOCHEMICAL SOCIETY TRANSACTIONS

Large scale expression and purification of recombinant RNA in Escherichia coli

(2011) Luc Ponchon et al.   METHODS

Silencing or Stimulation? siRNA Delivery and the Immune System

(2011) Kathryn A. Whitehead et al.   Annual Review of Chemical and Biomolecular Engineering

Rational design of cationic lipids for siRNA delivery

(2010) Sean C Semple et al.   NATURE BIOTECHNOLOGY

A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold

(2009) Luc Ponchon et al.   Nature Protocols

Microfluidic Platform for Controlled Synthesis of Polymeric Nanoparticles

(2008) Rohit Karnik et al.   NANO LETTERS