Article
Pharmacology & Pharmacy
Serah Kimani, Suparna Chakraborty, Ikponmwosa Irene, Jo de la Mare, Adrienne Edkins, Andre du Toit, Ben Loos, Angelique Blanckenberg, Annick Van Niekerk, Leticia Costa-Lotufo, K. N. ArulJothi, Selwyn Mapolie, Sharon Prince
Summary: The study investigated the anti-cancer activity of a palladium complex, BTC2, in human breast cancer cells, demonstrating its selectivity, inhibition of cancer cell migration, and suppression of cancer stem cell activity. BTC2 also induced DNA double strand breaks, triggered cell cycle arrests, and activated multiple cell death pathways, showing promising anti-breast cancer activity both in vitro and in vivo.
BIOCHEMICAL PHARMACOLOGY
(2021)
Review
Oncology
Chiara Pedicone, Shea T. Meyer, John D. Chisholm, William G. Kerr
Summary: This review highlights the crucial role of SHIP1 and SHIP2 in cancer signaling and immune response to cancer, as well as the potential use of SHIP inhibitors and agonists in cancer therapeutics. Small molecule inhibitors and agonists targeting SHIP1 and SHIP2 have shown promising results in pre-clinical models of disease, including cancer and tumor immunotherapy. The molecular structure, binding site, and efficacy of these SHIP inhibitors are discussed in detail for potential use in cancer treatment.
Article
Oncology
Hengrui Liu, James P. Dilger, Jun Lin
Summary: Lidocaine inhibits viability and migration of multiple breast cancer cell lines, potentially by regulating the TRPM7 channel. The study findings support the potential clinical application of lidocaine in the treatment of breast cancer.
Article
Cell Biology
Xiangchan Hou, Le Tang, Xiayu Li, Fang Xiong, Yongzhen Mo, Xianjie Jiang, Xiangying Deng, Miao Peng, Pan Wu, Mengyao Zhao, Jiawei Ouyang, Lei Shi, Yi He, Qijia Yan, Shanshan Zhang, Zhaojian Gong, Guiyuan Li, Zhaoyang Zeng, Fuyan Wang, Can Guo, Wei Xiong
Summary: Breast cancer is the most common malignant tumor in women, with KCNK6 being significantly overexpressed in breast cancer cells and promoting their proliferation, invasion, and migration abilities. Targeting KCNK6 may offer a novel therapeutic strategy for breast cancer treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Cong Zhang, Zhuowen Yang, Peng Zhou, Muxin Yu, Baorong Li, Yingmiao Liu, Jiaqi Jin, Wenhui Liu, Haijiao Jing, Jingwen Du, Jie Tian, Zhiyu Zhao, Jianxin Wang, Yinzhu Chu, ChunMei Zhang, Valerie A. Novakovic, Jialan Shi, Changjun Wu
Summary: The study found that neoadjuvant chemotherapy significantly increased the expression of PS+ BCMPs in patient plasma, which was associated with a rapid increase in procoagulant activity. Treatment with lactadherin reduced the adhesion of BCMPs and their procoagulant activity. Injection of BCMPs in mice induced a hypercoagulable state, reducing fibrinogen levels and promoting thrombus formation.
Article
Cell Biology
Mengna Zhang, Lingxian Zhang, Ajun Geng, Xiao Li, Yu Zhou, Liming Xu, Yi Arial Zeng, Jinpeng Li, Cheguo Cai
Summary: In this study, the researchers investigated the function of cyclin-dependent kinase 14 (CDK14) in mammary development and breast cancer progression. The results showed that CDK14 is expressed in mammary basal cells and elevated in triple negative breast cancer (TNBC). CDK14 knockdown or inhibition was found to suppress mammary regeneration and the progression of TNBC.
Article
Biochemistry & Molecular Biology
Peigang Liang, Jiaqi Zhang, Yuchen Wu, Shanyuan Zheng, Zhaopeng Xu, Shuo Yang, Jinfang Wang, Suibin Ma, Li Xiao, Tianhui Hu, Wenxue Jiang, Chaoqun Huang, Qiong Xing, Mondira Kundu, Bo Wang
Summary: This study reveals a new role for the autophagy-inducing serine/threonine kinases ULK1 and ULK2 in mechanotransduction. ULK1/2 activity inhibits actin stress fiber and focal adhesion assembly, impairing cell contraction and migration. The findings provide insights into the mechanical properties of breast cancer cells and their response to mechanical stimuli.
Article
Cell Biology
Saima Usman, Ahmad Jamal, Antesar Bushaala, Naushin H. H. Waseem, Hebah Al-Dehlawi, William Andrew Yeudall, Muy-Teck Teh, Hemanth Tummala, Ahmad Waseem
Summary: In advanced metastatic cancers, the expression of vimentin is frequently observed and it is associated with reduced patient survival and poor prognosis. Vimentin can change the shape of breast cancer cells and increase cell migration by suppressing epithelial characteristics and affecting gene expression related to cell-cell adhesion. Additionally, the presence of vimentin can alter nuclear architecture and downregulate genes involved in cell-cell adhesion, leading to increased cell migration.
Article
Biochemistry & Molecular Biology
Agne Janoniene, Linas Mazutis, Daumantas Matulis, Vilma Petrikaite
Summary: Inhibition of CA IX by VD11-4-2 decreases movement speed in breast cancer cells without affecting non-cancerous cells. The inhibitor hinders cells from reaching their maximum speed and reduces cell movement towards growth factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Narendra Thapa, Tianmu Wen, Vincent L. Cryns, Richard A. Anderson
Summary: This article focuses on the processes of cell adhesion and migration in cancer cells, specifically discussing the reorganization of microtubule cytoskeleton, cell polarity, and phosphoinositide signaling and their impact on adhesion and migration.
Article
Cell Biology
Juliana Haydee Enrique Steinberg, Fabiana Alejandra Rossi, Roberto Magliozzi, Laurensia Yuniati, Matteo Santucci, Mario Rossi, Daniele Guardavaccaro, Angela Lauriola
Summary: The research demonstrates that SHARP1 functions as a suppressor of metastasis in TNBC. It inhibits the invasive phenotype of TNBC by blocking hypoxia-inducible factors. Additionally, targeting the beta TrCP-dependent degradation of SHARP1 shows potential as a therapeutic strategy in TNBC.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Mohsen Fathi, Melisa Martinez-Paniagua, Ken Chen, Ali Rezvan, Melisa J. Montalvo, Vakul Mohanty, Sendurai A. Mani, Navin Varadarajan
Summary: Excitatory cells play an important role in the neuronal circuits underlying feeding behavior in Drosophila. We show here that the activity of a subset of excitatory neurons expressing either neuropeptide F (NPF) or short neuropeptide F (sNPF) in the adult Drosophila brain is necessary for sleep homeostasis. These neurons promote sleep primarily through output signals downstream of the mushroom body output neurons.
Article
Cell Biology
Grace Cai, Anh Nguyen, Yashar Bashirzadeh, Shan-Shan Lin, Dapeng Bi, Allen P. Liu
Summary: This study reveals the importance of cell-cell adhesion and substrate traction in cellular unjamming transitions under compressive stress. Strengthened intercellular adhesion and attenuation of traction forces contribute to the fluidization of cell layer, which may impact collective cell motion in tumor development and breast cancer progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Tung-Wei Hsu, Hsin-An Chen, Po-Hsiang Liao, Yen-Hao Su, Ching-Feng Chiu, Chih-Yang Huang, Yu-Jung Lin, Chih-Chiang Hung, Ming-Hsin Yeh, Shian-Ying Sun, Chih-Ming Su
Summary: The study investigated the role of Dicer in regulating the migration, invasion, and stemness of breast cancer cells. The findings revealed that Dicer expression levels were associated with breast cancer risk, and inhibition of Dicer enhanced the migratory, invasive, and cancer stem cell properties of breast cancer cells.
Article
Multidisciplinary Sciences
Maria De Luca, Roberta Romano, Cecilia Bucci
Summary: The decrease of V1G1 and RAB7 in highly invasive breast cancer cells plays a key role in cancer progression, while modulation of V1G1 affects cell migration and matrix metalloproteinase activation. V1G1 also modulates EGFR stability and downstream signaling pathways, influencing cell motility factors.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Lukas Balek, Pavel Nemec, Peter Konik, Michaela Kunova Bosakova, Miroslav Varecha, Iva Gudernova, Jirina Medalova, Deborah Krakow, Pavel Krejci
CELLULAR SIGNALLING
(2018)
Article
Multidisciplinary Sciences
Michaela Kunova Bosakova, Alexandru Nita, Tomas Gregor, Miroslav Varecha, Iva Gudernova, Bohumil Fafilek, Tomas Barta, Neha Basheer, Sara P. Abraham, Lukas Balek, Marketa Tomanova, Jana Fialova Kucerova, Juraj Bosak, David Potesil, Jennifer Zieba, Jieun Song, Peter Konik, Sohyun Park, Ivan Duran, Zbynek Zdrahal, David Smajs, Gert Jansen, Zheng Fu, Hyuk Wan Ko, Ales Hampl, Lukas Trantirek, Deborah Krakow, Pavel Krejci
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Tomas Gregor, Michaela Kunova Bosakova, Alexandru Nita, Sara P. Abraham, Bohumil Fafilek, Nicole H. Cernohorsky, Jan Rynes, Silvie Foldynova-Trantirkova, Daniela Zackova, Jiri Mayer, Lukas Trantirek, Pavel Krejci
CELLULAR AND MOLECULAR LIFE SCIENCES
(2020)
Article
Medicine, Research & Experimental
Michaela Bosakova, Sara P. Abraham, Alexandru Nita, Eva Hruba, Marcela Buchtova, S. Paige Taylor, Ivan Duran, Jorge Martin, Katerina Svozilova, Tomas Barta, Miroslav Varecha, Lukas Balek, Jiri Kohoutek, Tomasz Radaszkiewicz, Ganesh Pusapati, Vitezslav Bryja, Eric T. Rush, Isabelle Thiffault, Deborah A. Nickerson, Michael J. Bamshad, Rajat Rohatgi, Daniel H. Cohn, Deborah Krakow, Pavel Krejci
EMBO MOLECULAR MEDICINE
(2020)
Article
Medicine, General & Internal
Maya Barad, Fabiana Csukasi, Michaela Bosakova, Jorge H. Martin, Wenjuan Zhang, S. Paige Taylor, Ralph S. Lachman, Jennifer Zieba, Michael Bamshad, Deborah Nickerson, Jessica X. Chong, Daniel H. Cohn, Pavel Krejci, Deborah Krakow, Ivan Duran
Article
Cell Biology
Takeshi Kimura, Michaela Bosakova, Yosuke Nonaka, Eva Hruba, Kie Yasuda, Satoshi Futakawa, Takuo Kubota, Bohumil Fafilek, Tomas Gregor, Sara P. Abraham, Regina Gomolkova, Silvie Belaskova, Martin Pesl, Fabiana Csukasi, Ivan Duran, Masatoshi Fujiwara, Michaela Kavkova, Tomas Zikmund, Josef Kaiser, Marcela Buchtova, Deborah Krakow, Yoshikazu Nakamura, Keiichi Ozono, Pavel Krejci
Summary: The study demonstrates that RBM-007 can effectively inhibit FGFR3 signaling, repair detrimental effects on cartilage and skeletal development, providing a potential therapeutic approach for achondroplasia and other FGFR3-related skeletal dysplasias.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Endocrinology & Metabolism
Eva Hruba, Michaela Kavkova, Linda Dalecka, Milos Macholan, Tomas Zikmund, Miroslav Varecha, Michaela Bosakova, Jozef Kaiser, Pavel Krejci, Maria Hovorakova, Marcela Buchtova
Summary: The Sprouty family genes are associated with disruptions in primary cilia structure and Hedgehog signaling defects in chondrocytes, leading to multiple pathologies resembling ciliopathies. Deficiency of Sprouty proteins in mice may serve as valuable models for studying the relationship between RTK overactivation and primary cilia dysfunction during skeletogenesis.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Review
Cell Biology
Alexandru Nita, Sara P. Abraham, Pavel Krejci, Michaela Bosakova
Summary: Primary cilium guides cell fate decisions and is controlled by signaling molecules like FGFR. Aberrant FGFR activity leads to abnormal cilia and deregulated signaling, contributing to genetic disorders and cancer. FGFR gene fusions may drive oncogenesis by affecting centrosome proteins and causing cilia loss.
Review
Anatomy & Morphology
Sara P. Abraham, Alexandru Nita, Pavel Krejci, Michaela Bosakova
Summary: Primary cilia are dynamic compartments that regulate various aspects of cellular signaling, including skeletal development and homeostasis. Skeletal ciliopathies are genetic disorders with a wide range of pathologies, and protein kinases in cilia play essential roles in bone physiology signaling pathways.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Endocrinology & Metabolism
Ivan Duran, Jennifer Zieba, Fabiana Csukasi, Jorge H. Martin, Davis Wachtell, Maya Barad, Brian Dawson, Bohumil Fafilek, Christina M. Jacobsen, Catherine G. Ambrose, Daniel H. Cohn, Pavel Krejci, Brendan H. Lee, Deborah Krakow
Summary: Osteogenesis imperfecta (OI) is a genetic disorder characterized by bone fragility and deformities. Endoplasmic reticulum (ER) stress has been identified as a contributing factor to OI pathogenesis. In this study, treatment with the chemical chaperone 4-phenylbutiric acid (4-PBA) was found to alleviate the phenotype of OI in mice, leading to improved growth, bone density, and reduced ER stress markers.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Review
Orthopedics
B. Fafilek, M. Bosakova, P. Krejci
Summary: Activating mutations in the FGFR3 receptor tyrosine kinase lead to the most common genetic dwarfism in humans, achondroplasia. A stable variant of the C-natriuretic peptide, vosoritide, has been approved as a treatment for achondroplasia, and other drugs targeting FGFR3 signaling are progressing through clinical trials. This review explores current therapeutics for achondroplasia, their mechanisms, potential cures, and options for repurposing these drugs for unrelated dwarfing conditions.
OSTEOARTHRITIS AND CARTILAGE
(2022)
Article
Biology
Kamila Weissova, Bohumil Fafilek, Tomasz Radaszkiewicz, Canan Celiker, Petra Machackova, Tamara Cechova, Jana Sebestikova, Ales Hampl, Vitezslav Bryja, Pavel Krejci, Tomas Barta
Summary: LuminoCell is an affordable, sensitive, and portable luminometer capable of real-time monitoring in-cell luciferase activity. It can detect the activity of major signaling pathways in cell cultures and is suitable for cytotoxicity assays and monitoring periodic circadian gene expression.
LIFE SCIENCE ALLIANCE
(2022)
Article
Genetics & Heredity
Martin Pesl, Hana Verescakova, Linda Skutkova, Jana Strenkova, Pavel Krejci
Summary: This study established a patient registry for ACH in the Czechia and Slovak Republic, collecting clinical information from 51 pediatric ACH patients over 6 years. The registry continues to collect data to monitor growth and other parameters for future therapy.
ORPHANET JOURNAL OF RARE DISEASES
(2022)
Article
Cell & Tissue Engineering
Jennifer Zieba, Kimberly N. Forlenza, Kelly Heard, Jorge H. Martin, Michaela Bosakova, Daniel H. Cohn, Stephen P. Robertson, Pavel Krejci, Deborah Krakow
Summary: Spondylocarpotarsal syndrome (SCT) is a rare musculoskeletal disorder characterized by short stature and vertebral, carpal, and tarsal fusions. This study found that SCT is caused by alterations in the TGF beta/BMP signaling pathway, and FLNB plays a key role in maintaining the balance of this signaling pathway in the intervertebral discs.
Letter
Medicine, General & Internal
Pavel Krejci
NEW ENGLAND JOURNAL OF MEDICINE
(2019)