The path to VICTORy – a beginner's guide to success using commercial research antibodies

When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions

(2018) Andrew R. M. Bradbury et al.   mAbs

The antibody horror show: an introductory guide for the perplexed

(2018) Simon. L. Goodman   New Biotechnology

Proof of the quantitative potential of immunofluorescence by mass spectrometry

(2017) Maria I Toki et al.   LABORATORY INVESTIGATION

Identification and verification of hybridoma-derived monoclonal antibody variable region sequences using recombinant DNA technology and mass spectrometry

(2017) Lmar Babrak et al.   MOLECULAR IMMUNOLOGY

The secret war against counterfeit science

(2017) David Cyranoski   NATURE

Should we ignore western blots when selecting antibodies for other applications?

(2017) Fridtjof Lund-Johansen et al.   NATURE METHODS

Principles for computational design of binding antibodies

(2017) Dror Baran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A trust approach for sharing research reagents

(2017) Aled Edwards et al.   Science Translational Medicine

Insufficient antibody validation challenges oestrogen receptor beta research

(2017) Sandra Andersson et al.   Nature Communications

In vitro-engineered non-antibody protein therapeutics

(2017) Rudo Simeon et al.   Protein & Cell

Phage display peptide libraries in molecular allergology: from epitope mapping to mimotope-based immunotherapy

(2016) J. Luzar et al.   ALLERGY

[Letter to the Editor] The need for improved education and training in research antibody usage and validation practices

(2016) Leonard P. Freedman et al.   BIOTECHNIQUES

Antibody Validation in Bioimaging Applications Based on Endogenous Expression of Tagged Proteins

(2016) Marie Skogs et al.   JOURNAL OF PROTEOME RESEARCH

Half of samples fail protein-blot tests

(2016) Matt Landry et al.   NATURE

Perspective: Science is still too closed

(2016) Aled Edwards   NATURE

How quality control could save your science

(2016) Monya Baker   NATURE

MetaMass, a tool for meta-analysis of subcellular proteomics data

(2016) Fridtjof Lund-Johansen et al.   NATURE METHODS

A proposal for validation of antibodies

(2016) Mathias Uhlen et al.   NATURE METHODS

RRIDs: A Simple Step toward Improving Reproducibility through Rigor and Transparency of Experimental Methods

(2016) Anita E. Bandrowski et al.   NEURON

High-accuracy modeling of antibody structures by a search for minimum-energy recombination of backbone fragments

(2016) Christoffer H. Norn et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Getting controls under control: the time is now for immunohistochemistry

(2015) Emina Emilia Torlakovic et al.   JOURNAL OF CLINICAL PATHOLOGY

The European antibody network's practical guide to finding and validating suitable antibodies for research

(2015) Giovanna Roncador et al.   mAbs

Antibody anarchy: A call to order

(2015) Monya Baker   NATURE

Antibodies: validate recombinants once

(2015) Andrew M. Bradbury et al.   NATURE

Reproducibility crisis: Blame it on the antibodies

(2015) Monya Baker   NATURE

A resource for cell line authentication, annotation and quality control

(2015) Mamie Yu et al.   NATURE

Reproducibility: Standardize antibodies used in research

(2015) Andrew Bradbury et al.   NATURE

Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation

(2015) Edyta Marcon et al.   NATURE METHODS

The Economics of Reproducibility in Preclinical Research

(2015) Leonard P. Freedman et al.   PLOS BIOLOGY

Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS)

(2015) Jennifer E. Gilda et al.   PLoS One

Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins

(2015) Nan Zhong et al.   PLoS One

CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited

(2014) Matthew A Helsby et al.   BMC CELL BIOLOGY

Translational researchers beware! Unreliable commercial immunoassays (ELISAs) can jeopardize your research

(2014) Ioannis Prassas et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Method Development: Agar Cell Pellets and Cell Pellet Arrays for Immunohistochemistry andIn SituHybridization

(2014) Nila Mehta et al.   JOURNAL OF HISTOTECHNOLOGY

The functional repertoire of rabbit antibodies and antibody discovery via next-generation sequencing

(2014) Sreekumar Kodangattil et al.   mAbs

False Biomarker Discovery due to Reactivity of a Commercial ELISA for CUZD1 with Cancer Antigen CA125

(2013) I. Prassas et al.   CLINICAL CHEMISTRY

Structural insights for engineering binding proteins based on non-antibody scaffolds

(2012) Ryan N Gilbreth et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Autoreactive antibodies raised by self derived de novo peptides can identify unrelated antigens on protein microarrays. Are autoantibodies really autoantibodies?

(2012) Kaitlin Kroening et al.   EXPERIMENTAL AND MOLECULAR PATHOLOGY

Raise standards for preclinical cancer research

(2012) C. Glenn Begley et al.   NATURE

Benefits and Pitfalls of Secondary Antibodies: Why Choosing the Right Secondary Is of Primary Importance

(2012) Colleen F. Manning et al.   PLoS One

High Affinity Humanized Antibodies without Making Hybridomas; Immunization Paired with Mammalian Cell Display and In Vitro Somatic Hypermutation

(2012) Audrey D. McConnell et al.   PLoS One

Matched rabbit monoclonal antibodies against  v-series integrins reveal a novel  v 3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumors

(2012) S. L. Goodman et al.   Biology Open

A roadmap to generate renewable protein binders to the human proteome

(2011) Karen Colwill et al.   NATURE METHODS

Believe it or not: how much can we rely on published data on potential drug targets?

(2011) Florian Prinz et al.   NATURE REVIEWS DRUG DISCOVERY

Advances in the production and downstream processing of antibodies

(2011) John H. Chon et al.   New Biotechnology

Antibody validation

(2010) Jennifer Bordeaux et al.   BIOTECHNIQUES

How accurate are cancer cell lines?

(2010) Brendan Borrell   NATURE

Correlation of mRNA and protein in complex biological samples

(2009) Tobias Maier et al.   FEBS LETTERS

A Community Standard Format for the Representation of Protein Affinity Reagents

(2009) David E. Gloriam et al.   MOLECULAR & CELLULAR PROTEOMICS

Discrepancy between mRNA and protein abundance: Insight from information retrieval process in computers

(2008) Degeng Wang   COMPUTATIONAL BIOLOGY AND CHEMISTRY

Commercial Antibodies: The Good, Bad, and Really Ugly

(2008) John R. Couchman   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

A Genecentric Human Protein Atlas for Expression Profiles Based on Antibodies

(2008) Lisa Berglund et al.   MOLECULAR & CELLULAR PROTEOMICS

Antibodypedia, a Portal for Sharing Antibody and Antigen Validation Data

(2008) Erik Björling et al.   MOLECULAR & CELLULAR PROTEOMICS

The epitope space of the human proteome

(2008) Lisa Berglund et al.   PROTEIN SCIENCE