Article
Biotechnology & Applied Microbiology
Minami Ogawa, Pablo Carmona-Jimenez, Teresa Garcia-Martinez, Jesus Valentin Jorrin-Novo, Juan Moreno, Maria Dolores Rey, Jaime Moreno-Garcia
Summary: Immobilized cell technologies (ICT) are commonly used in brewing to improve cell stability, flexibility, and control. Yeast biocapsules, a new form of ICT, were found to be more suitable for cell reutilization than alginate beads but had a greater impact on beer clarity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Chemistry, Analytical
Jiri Salplachta, Anna Kubesova, Dana Moravcova, Filip Dusa
Summary: This article mainly focuses on the application of capillary electrophoresis (CE) in the analysis of yeasts and filamentous molds. It provides a brief introduction to the theoretical basis of CE, discusses the surface properties of fungal cells in relation to their charge, and presents the major difficulties encountered in CE of fungal species and the corresponding approaches to overcome these difficulties. The article also highlights some recent advances in fungal CE.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Rafaella Silva Aredes, Fernando Cunha Peixoto, Leandro Alcoforado Sphaier, Flavia Ferreira de Carvalho Marques
Summary: This study used capillary zone electrophoresis method with UV-vis detection in combination with Principal Component Analysis to classify craft beer, monitoring six amino acids in wort and finished beer. Differentiation among samples based on AA profiles and protein cleavage mechanism was successfully achieved.
Article
Food Science & Technology
Marek Zdaniewicz, Aneta Pater, Aleksandra Knapik, Robert Dulinski
Summary: The research shows that adding 10% oat malt does not negatively impact the brewing process, but higher oat malt content can result in decreased wort quantity, extract content, alcohol concentration, and intensified beer color.
JOURNAL OF CEREAL SCIENCE
(2021)
Article
Food Science & Technology
Martina Daute, Frances Jack, Barry Harrison, Graeme Walker
Summary: The study examined the impact of different wort pretreatments on fermentation performance and flavor development in Scotch whisky distillates, finding that boiled and autoclaved wort led to increased levels of esters and higher alcohols, while filtered wort had lower congeners. However, all three pretreatments resulted in decreased alcohol yields, with frozen or fresh wort recommended for small-scale fermentation trials.
Article
Food Science & Technology
Li Li, Ye Zhang, Jia Zheng, Mao Li, Hong Wang, Jianhang Wu, Xuyan Zong
Summary: This study explored the impact of different mashing conditions on the quality of qingke wort. It was found that smaller crushed particle sizes resulted in higher fermentable sugar content in the wort. Enzyme treatment further increased the fermentable sugar content, enhancing the fermentation capacity. Additionally, crushing particle size also affected the antioxidant properties of the wort.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Jingjing Xue, Yiren Cao, Genhan Zha, Zixian Yu, Yuxin Wang, Weiwen Liu, Jicun Ren, Hua Xiao, Qiang Zhang, Chengxi Cao, Li Wei
Summary: In this study, a quadruple UV LED array was designed to directly irradiate gel electrophoresis chips at 275 nm. Simple detection setup using a 365 nm filter, UV camera lens, and CCD was integrated with the small gel electrophoresis chip to construct the IFI-GEC device. The LED array exhibited good evenness and enabled online imaging of the entire gel electrophoresis chip. The reproducible IFI-GEC detection was completed within 10 minutes with a low LOD of 40 ng for lysozyme detection, demonstrating the potential of the IFI-GEC device for stain-free online and portable protein detection.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Weiwen Liu, Ziqi Liang, Yuanyu Wang, Jun Cao, Qiang Zhang, Xiaoping Liu, Yuxing Wang, Chengxi Cao
Summary: A novel online multi-gear capacitively coupled contactless conductivity detection (M-(CD)-D-4) sensor was proposed in this study, with successful development of the model, theory, and digital computation, demonstrating promising results in liquid chromatography experiments.
Article
Chemistry, Multidisciplinary
Velu Duraisamy, Velayutham Sudha, Kuppusamy Annadurai, Sakkarapalayam Murugesan Senthil Kumar, Rangasamy Thangamuthu
Summary: The development of active and defective sites in mesoporous carbon materials is crucial for high-performance sensors. NHCS-1000, with improved surface area, porosity, and N-doping, showed enhanced electrocatalytic activity and was successfully utilized for the simultaneous detection of multiple analytes.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Analytical
Audrius Maruska, Tomas Drevinskas, Mantas Stankevicius, Kristina Bimbiraite-Surviliene, Vilma Kaskoniene, Linas Jonusauskas, Roaldas Gadonas, Staffan Nilsson, Olga Kornysova
Summary: This work describes the design and characterization of a multi-cell capacitively coupled contactless conductivity detection system, capable of simultaneously acquiring data from multiple detector cells and easily upgradable to 64 detection cells. The system demonstrated sensitivity to low concentrations of various solutions in water and the ability to distinguish different mixtures of organic solvents. It is expected to be used in conjunction with multi-channel separation devices for monitoring simultaneous processes.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Wanxin Li, Jiaxin Sun, Yao Xin, Yu Han, Yanyang Sun, Aijun Li, Zhi Wang
Summary: A novel nanozyme was synthesized for the detection of resveratrol. This nanozyme exhibited excellent peroxidase-like activity and the colorimetric reaction could be completed within 20 minutes.
ANALYTICAL METHODS
(2023)
Review
Chemistry, Analytical
Theo Lienard-Mayor, Myriam Taverna, Stephanie Descroix, Thanh Duc Mai
Summary: This study provides a comprehensive overview of different strategies for combining droplet-based sample handling and preparation with electrophoretic separation. It focuses on three principal interfacing strategies, including droplets in immiscible phases, digital microfluidics with electrowetting-on-dielectric principle, and inkjet droplet generation. Different instrumental developments, pros and cons of designs, and application demonstrations of droplet-interfaced electrokinetic strategies are discussed.
ANALYTICA CHIMICA ACTA
(2021)
Review
Chemistry, Analytical
Sille Stepanova, Vaclav Kasicka
Summary: This paper provides a detailed overview of recent developments in separation and analysis of proteins using capillary electromigration (CE) methods. It discusses sample preparation, suppression of protein sorption, control of electroosmotic flow, specific CE methods for protein separation, and protein identification and quantification. Additionally, it showcases several novel applications of CE methods in protein analysis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Hongyu Nie, Zhihui Li, Xiaokun Wang, Rongmeng Gu, Hongyan Yuan, Yong Guo, Dan Xiao
Summary: In this study, a multi-input capacitively coupled contactless conductivity detector (MICD)-D-4 was proposed with high sensitivity, and further improved by combining it with differential detection to form (DFMICD)-D-4. The new detector showed improved performance compared to traditional single-input and double-input detectors, with a higher response-to-baseline ratio and a low limit of detection (0.7 nM). It can be applied to the detection of low-conductivity solutions and total dissolved solids (TDS) analysis, demonstrating its versatility and superiority over standard conductivity meters.
Review
Chemistry, Analytical
Laszlo Hajba, Sunkyung Jeong, Doo Soo Chung, Andras Guttman
Summary: This review provides an overview of the fundamental principles, methodologies, strengths, and weaknesses of capillary gel electrophoresis of proteins. It covers the historical background, new developments, and applications in biopharmaceutical, biomedical, food, and agriculture fields. Different subsets of the method and relevant protein labeling techniques are critically discussed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Instruments & Instrumentation
Janos Mink, Andras Stirling, Dickson O. Ojwang, Gunnar Svensson, Judith Mihaly, Csaba Nemeth, Markus Drees, Laszlo Hajba
APPLIED SPECTROSCOPY REVIEWS
(2019)
Review
Pharmacology & Pharmacy
Laszlo Hajba, Akos Szekrenyes, Beata Borza, Andras Guttman
DRUG DISCOVERY TODAY
(2018)
Review
Chemistry, Analytical
L. Hajba, A. Guttman
Summary: Capillary electrochromatography (CEC) is a powerful hybrid separation technique combining capillary electrophoresis and capillary chromatography, which can effectively address the analytical challenges in proteomics and glycomics. This paper reviews recent developments in capillary electrochromatography of proteins and carbohydrates, discussing different column types, coating methods, and porogenic solvent combinations for monolithic column fabrication. Application examples of CEC in process analytical technology for biopharmaceuticals and biomarker discovery are also presented.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2021)
Review
Medicine, General & Internal
Szabo Miklos, Kun Renata, Hajba Laszlo, Koncz Roland, Guttman Andras, Csanky Eszter
Review
Medicine, Research & Experimental
Lazlo Hajba, Andras Guttman
CURRENT MOLECULAR MEDICINE
(2020)
Review
Medicine, Research & Experimental
Beata Borza, Laszlo Hajba, Andras Guttman
CURRENT MOLECULAR MEDICINE
(2020)
Editorial Material
Medicine, Research & Experimental
Laszlo Hajba
CURRENT MOLECULAR MEDICINE
(2020)
Article
Spectroscopy
Janos Mink, Lena Staiger, Maximilian Muhr, Christian Gemel, Markus Drees, Laszlo Hajba, Judith Mihaly, Csaba Nemeth, Boris Lokshin, Karina Hemmer, Max Schutz, Mirza Cokoja, Roland A. Fischer
Summary: Through Raman and infrared spectroscopy and theoretical calculations, the structure-function relationship of two new intermetallic Ni/Ga clusters has been investigated. Cluster 1B exhibited stronger metal-ligand bonding and higher hydrogen or deuterium uptake ability compared to cluster 1A, supporting its higher hydrogenation activity.
JOURNAL OF RAMAN SPECTROSCOPY
(2021)
Article
Green & Sustainable Science & Technology
Monika Meiczinger, Bela Varga, Lana Wolmarans, Laszlo Hajba, Viola Somogyi
Summary: This study investigates the use of cyclodextrin to immobilize laccase enzyme and improve its stability for the removal of micropollutants. The results show that alpha-cyclodextrin immobilization has the best effect, increasing the half-life of laccase and maintaining its activity under various conditions. The nanocomposite of alpha-cyclodextrin-laccase also shows better removal efficiency of diclofenac compared to free laccase.
CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY
(2022)
Review
Chemistry, Analytical
Laszlo Hajba, Sunkyung Jeong, Doo Soo Chung, Andras Guttman
Summary: This review provides an overview of the fundamental principles, methodologies, strengths, and weaknesses of capillary gel electrophoresis of proteins. It covers the historical background, new developments, and applications in biopharmaceutical, biomedical, food, and agriculture fields. Different subsets of the method and relevant protein labeling techniques are critically discussed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Sunkyung Jeong, Laszlo Hajba, Andras Guttman, Jiwoong Seol, Doo Soo Chung
Summary: Capillary electrophoresis (CE) is a powerful analytical technique for complex samples, but suffers from poor sensitivity and sample destacking. This review discusses the use of in-line solid-phase microextraction (SPME) and liquid-phase microextraction (LPME) techniques in CE, without the need for complex modifications to the commercial CE instrument. In-line coupling allows for reduced sample loss and increased enrichment factors, making it a valuable tool for analyzing trace substances in complex biological matrices.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Miklos Szabo, Laszlo Hajba, Renata Kun, Andras Guttman, Eszter Csanky
CURRENT MEDICINAL CHEMISTRY
(2020)
Review
Instruments & Instrumentation
Janos Mink, Judith Mihaly, Csaba Nemeth, Peter Nemeth, Markus Drees, Boris V. Lokshin, Moritz Wolf, Ian S. Butler, Laszlo Hajba
APPLIED SPECTROSCOPY REVIEWS
(2018)
Article
Biochemical Research Methods
Gabor Jarvas, Tamas Varga, Marton Szigeti, Laszlo Hajba, Peter Furjes, Istvan Rajta, Andras Guttman
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
