Article
Multidisciplinary Sciences
Alfredo Erazo-Oliveras, Monica Munoz-Vega, Mohamed Mlih, Venkataramana Thiriveedi, Michael L. Salinas, Jaileen M. Rivera-Rodriguez, Eunjoo Kim, Rachel C. Wright, Xiaoli Wang, Kerstin K. Landrock, Jennifer S. Goldsby, Destiny A. Mullens, Jatin Roper, Jason Karpac, Robert S. Chapkin
Summary: This study reveals the role of mutant APC in colorectal cancer by disrupting plasma membrane sterol homeostasis and modulating Wnt signaling hubs. It has been found that Wnt signaling originates from dynamic nano-assemblies at the plasma membrane. The oncogenic APC increases plasma membrane free cholesterol and rigidity, leading to overactivation of Wnt signaling in the colon. Exogenous free cholesterol can disrupt plasma membrane homeostasis and drive Wnt signaling in wildtype APC background. These findings provide a link between oncogenic APC, loss of plasma membrane homeostasis, and CRC development.
NATURE COMMUNICATIONS
(2023)
Review
Microbiology
Ines Ripa, Sabina Andreu, Jose Antonio Lopez-Guerrero, Raquel Bello-Morales
Summary: Membrane rafts are small dynamic domains enriched with cholesterol and sphingolipids that play essential roles in processes such as virus entry. They participate in virus attachment, recruitment to the cell surface, and entry mechanisms used by viruses. This review will introduce the specific role of rafts in viral entry and summarize their participation in the entry process of enveloped and non-enveloped viruses.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Cell Biology
Alessio D'Alessio
Summary: This review article provides a comprehensive account of the historical advancements in caveolae research, including the understanding of caveolin family proteins and their roles in cellular functions. It also examines the consequences of a dysfunctional caveolar network in the development of human diseases.
Article
Physics, Multidisciplinary
Taras Sych, Ramin Omidvar, Rafael Ostmann, Thomas Schubert, Annette Brandel, Ludovic Richert, Yves Mely, Josef Madl, Winfried Roemer
Summary: The dynamic reorganization of lipid rafts is important in host cell infection by human pathogens. Bacteria induce this reorganization through interactions with glycosphingolipids (GSLs). The authors studied how the bacterial lectin LecA affects the nanometric organization of membranes and found that it causes dispersion of liquid-ordered domains and surface deformation, providing insights into P. aeruginosa infection.
COMMUNICATIONS PHYSICS
(2023)
Article
Chemistry, Medicinal
Keying Liang, Shengsheng Mei, Xiangzheng Gao, Shanshan Peng, Jinbiao Zhan
Summary: The study has identified the caveolae/lipid-raft mediated endocytosis pathway of T-DM1, demonstrating that modulation of this pathway may improve the therapeutic effect of T-DM1.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Virology
Yuyang Wang, Yangyang Zhang, Wei Zuo, Zongyi Bo, Chengcheng Zhang, Xiaorong Zhang, Yantao Wu
Summary: In this study, it was demonstrated that the sigma B protein of ARV interacts with Cav-1 during lipid raft-mediated endocytosis, highlighting the significance of Cav-1 in ARV entry.
Article
Biochemistry & Molecular Biology
Xiaowei Li, Wenhua Zhu, Meiyang Fan, Jing Zhang, Yizhao Peng, Fumeng Huang, Nan Wang, Langchong He, Lei Zhang, Rikard Holmdahl, Liesu Meng, Shemin Lu
Summary: This study found that SARS-CoV-2 enters human cells through ACE2 and is sensitive to the pH of endosomes/lysosomes in HEK293T cells, with infection dependent on cholesterol-rich lipid rafts instead of other pathways. These findings expand the understanding of the entry mechanism of SARS-CoV-2 and suggest a new strategy for combating the virus.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Xue Pan, Patricio Perez-Henriquez, Jaimie M. Van Norman, Zhenbiao Yang
Summary: Cell polarity is vital for various biological processes in plants, but the underlying molecular and cellular mechanisms are not fully understood. Recent studies suggest that membrane protein/lipid nanodomains play a critical role in polarized morphogenesis. The regulation of spatiotemporal dynamics of signaling nanodomains remains a key question for achieving robust cell polarization in plants.
Review
Biology
Giuseppe Miserocchi
Summary: The lung responds promptly to conditions that cause edema through functional adaptations. This review provides evidence for early signaling transduction in endothelial lung cells in two animal models of edema. The role of membrane rafts, such as caveolae and lipid rafts, in signaling transduction is discussed. The changes in lipid composition of the plasma membrane trigger the signaling process in response to changes in the pericellular microenvironment caused by edema.
Review
Virology
Yee Jing Gee, Yi Lin Sea, Sunil Kumar Lal
Summary: This review focuses on the interactions between viruses and lipid rafts at distinct stages of infection. It discusses the mechanisms of lipid raft modification exploited by viruses and highlights the importance of virus-raft interactions in viral infections. Researchers have discovered antivirals that target virus-raft interactions and therapeutic modulations of lipid rafts as potential antiviral interventions.
REVIEWS IN MEDICAL VIROLOGY
(2023)
Review
Cell Biology
Rosa Vona, Elisabetta Iessi, Paola Matarrese
Summary: Cholesterol is crucial for cell membrane structure and plays a key role in regulating cell signaling pathways. Its homeostasis is tightly regulated and dysregulation may be linked to cardiovascular diseases and cancer development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Sachin Surve, Simon C. Watkins, Alexander Sorkin
Summary: The study demonstrated that endogenous KRAS and NRAS are predominantly localized to the plasma membrane and do not colocalize with endosomal EGFR upon EGF stimulation. It was suggested that a small pool of surface EGFRs sustain signaling within the RAS-ERK1/2 pathway.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biophysics
Sebastian V. Morales, Ahmad Mahmood, Jacob Pollard, Janice Mayne, Daniel Figeys, Paul W. Wiseman
Summary: Membrane cholesterol-rich domains play a role in regulating the activity of low-density lipoprotein receptor (LDLR), which is involved in maintaining cellular cholesterol homeostasis. High membrane cholesterol concentrations lead to sequestration of LDLR in cholesterol-rich domains, reducing its ability to bind and internalize LDL particles. Reduced membrane cholesterol levels result in increased binding affinity between LDL and its receptor, and LDLR transits to nascent clathrin-coated domains for internalization.
BIOPHYSICAL JOURNAL
(2023)
Article
Microbiology
Shuying Chen, Fan Yang, Zixiang Zhu, Weijun Cao, Kaiqi Lian, Wei Zhang, Zhijian Zhu, Jijun He, Jianhong Guo, Xiangtao Liu, Bin Zhou, Haixue Zheng
Summary: The study reveals that foot-and-mouth disease virus can enter cells through endocytosis and endosomal pathways, even in the absence of integrin and heparan sulfate receptors. Inhibition of endocytosis can block viral internalization, while the acidic environment and the presence of dynamin are crucial for infection.
VETERINARY MICROBIOLOGY
(2022)
Article
Virology
Puxian Fang, Jiansong Zhang, Huichang Zhang, Sijin Xia, Jie Ren, Liyuan Tian, Dongcheng Bai, Liurong Fang, Shaobo Xiao
Summary: This study demonstrates that PDCoV uses clathrin-mediated endocytosis and macropinocytosis to enter porcine intestinal epithelial cells, revealing new insights into the viral entry mechanisms. The research also provides important information on the cellular pathways involved in PDCoV infection, which may guide future antiviral drug development.
JOURNAL OF VIROLOGY
(2021)
Article
Oncology
L. Sun, Y. Shi, G. Wang, X. Wang, S. Zeng, S. E. Dunn, G. D. Fairn, Y-J Li, D. E. Spaner
Article
Biochemistry & Molecular Biology
Minhyoung Lee, Gregory D. Fairn
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Cell Biology
Mira Sohn, Marek Korzeniowski, James P. Zewe, Rachel C. Wills, Gerald R. V. Hammond, Jana Humpolickova, Lukas Vrzal, Dominika Chalupska, Vaclav Veverka, Gregory D. Fairn, Evzen Boura, Tamas Balla
JOURNAL OF CELL BIOLOGY
(2018)
Article
Cell Biology
Stella M. Lu, Gregory D. Fairn
Editorial Material
Biology
Kevin C. Courtney, Karen Y. Y. Fung, Frederick R. Maxfield, Gregory D. Fairn, Xiaohui Zha
Article
Microbiology
Joel M. J. Tan, Nora Mellouk, Suzanne E. Osborne, Dustin A. Ammendolia, Diana N. Dyer, Ren Li, Diede Brunen, Jorik M. van Rijn, Ju Huang, Mark A. Czuczman, Marija A. Cemma, Amy M. Wons, Christopher M. Yip, Ramnik J. Xavier, Donna A. MacDuff, Fulvio Reggiori, Jayanta Debnath, Tamotsu Yoshimori, Peter K. Kim, Gregory D. Fairn, Etienne Coyaud, Brian Raught, Aleixo M. Muise, Darren E. Higgins, John H. Brumell
NATURE MICROBIOLOGY
(2018)
Article
Physiology
Hira Raheel, Siavash Ghaffari, Negar Khosraviani, Victoria Mintsopoulos, Derek Auyeung, Changsen Wang, Yun Hye Kim, Brendan Mullen, Hoon-Ki Sung, May Ho, Gregory Fairn, Dante Neculai, Maria Febbraio, Bryan Heit, Warren L. Lee
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2019)
Review
Cell Biology
Johannes Westman, Bernhard Hube, Gregory D. Fairn
CELLULAR MICROBIOLOGY
(2019)
Article
Cell Biology
Philip P. Ostrowski, Spencer A. Freeman, Gregory Fairn, Sergio Grinstein
DEVELOPMENTAL CELL
(2019)
Review
Cell Biology
Michael G. Sugiyama, Gregory D. Fairn, Costin N. Antonescu
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Yanbo Yang, Miriam Park, Masashi Maekawa, Gregory D. Fairn
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Fernando Montano-Rendon, Glenn F. W. Walpole, Matthias Krause, Gerald R. V. Hammond, Sergio Grinstein, Gregory D. Fairn
Summary: PI 3-kinase is crucial for phagocytosis, with PI3,4P2, Lamellipodin, and VASP playing essential roles in actin-driven pseudopod extension and particle engulfment. Phosphoinositides are key regulators of vesicular traffic and signaling during phagocytosis, with locally generated PtdIns(3,4)P-2 influencing pseudopod progression and phagocytosis. Lamellipodin and VASP are important actin-regulatory proteins involved in phagosome formation downstream of PtdIns(3,4)P-2.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
John Abousawan, Laura A. Orofiamma, Gregory D. Fairn, Costin N. Antonescu
Summary: The epidermal growth factor receptor (EGFR) controls many cellular functions by undergoing dimerization, phosphorylation, and activation of signals including the PI3K-Akt pathway upon ligand binding. The role of flotillin nanodomains in EGFR signaling remains unclear, as some studies suggest their involvement while others show limited effect. This study reveals that HER2 expression alters EGFR signaling duration and requires flotillin-1 and c-Src for Akt activation and cell proliferation.
JOURNAL OF CELL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Michael G. Sugiyama, Aidan I. Brown, Jesus Vega-Lugo, Jazlyn P. Borges, Andrew M. Scott, Khuloud Jaqaman, Gregory D. Fairn, Costin N. Antonescu
Summary: Using single particle tracking, the authors discovered that EGFR is more confined when bound to ligands, and this confinement is regulated by clathrin and tetraspanin nanodomains. Ligand binding to unliganded EGFR occurs mainly in tetraspanin nanodomains, and disrupting these nanodomains impairs ligand binding and alters the receptor's ectodomain conformation. This study reveals a mechanism by which EGFR confinement within tetraspanin nanodomains regulates ligand binding and signaling.
NATURE COMMUNICATIONS
(2023)