Article
Cell Biology
Dazhuang Miao, Yan Wang, Yunhe Jia, Jinxue Tong, Shixiong Jiang, Lixiu Liu
Summary: This study identified the upregulation of ZRANB1 in CRC patients, correlating with advanced tumor stage and poor survival. ZRANB1 increased Sox9 stability in CRC cells, promoting stem-cell-like features and activating the Wnt/beta-catenin pathway. These findings suggest ZRANB1 as a potential molecular target for novel CRC therapies.
CELLULAR SIGNALLING
(2022)
Article
Anatomy & Morphology
Taozhi Deng, Ping Zhong, Runlong Lou, Xiaojun Yang
Summary: RNF220 is upregulated in gastric cancer and plays a role in its development. Inhibition of RNF220 expression can suppress the growth and stemness of gastric cancer cells by regulating the USP22/Wnt/β-catenin signaling pathway.
Article
Pharmacology & Pharmacy
Zhibao Zheng, Na Luan, Kai Tu, Feiyan Liu, Jianwei Wang, Jianguo Sun
Summary: PCDH7 expression in colorectal cancer cells is positively correlated with cell proliferation and drug resistance, while negatively correlated with cell migration and invasion. PCDH7 mediates drug resistance by inhibiting cell apoptosis through upregulating Mcl-1 expression and activating the Wnt signaling pathway. The Mcl-1 inhibitor S63845 can attenuate the anti-apoptotic effect of PCDH7 and sensitizes PCDH7-overexpressing colorectal cancer cells to ABT-263.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Plant Sciences
Weijia Zhang, Chang Peng, Jiahui Yan, Pengting Chen, Cheng Jiang, Shuyi Sang, Yuemei Yuan, Yanjun Hong, Meicun Yao
Summary: This study demonstrated that the aqueous extract of Sanguisorba officinalis Linn. could inhibit the growth and metastasis of colorectal cancer cells by suppressing the Wnt pathway, providing potential for clinical application as an anti-CRC drug.
Article
Biochemistry & Molecular Biology
Yuyuan Zi, Liyu Liu, Jie Gao, Xu Xu, Yidi Guan, Zhuoxian Rong, Zhen Cao, Mengwei Li, Zimei Zeng, Qi Fan, Feiyu Tang, Junju He, Dan Feng, Jionghuang Chen, Yuedi Dai, Yufeng Huang, Yingjie Nie, Haiping Pei, Qingping Cai, Zhi Li, Lunquan Sun, Yuezhen Deng
Summary: This study finds that pancreatic progenitor cell differentiation and proliferation factor (PPDPF) is upregulated in colorectal cancer (CRC) and is significantly correlated with TNM stages and survival time. Knockout of PPDPF in the intestinal epithelium shortens crypts, decreases the number of stem cells, and inhibits CRC occurrence. Mechanistically, PPDPF interacts with CK1 alpha, disrupting its binding to Axin and activating the Wnt/beta-catenin pathway. Inflammatory signals mediated by IL6/JAK2 phosphorylate PPDPF, stabilizing the protein. Overall, PPDPF is a key molecule connecting inflammation to the Wnt/beta-catenin signaling pathway in CRC carcinogenesis and progression, suggesting it as a potential therapeutic target.
Article
Biochemistry & Molecular Biology
Yung-Fu Wu, Chih-Yang Wang, Wan-Chun Tang, Yu-Cheng Lee, Hoang Dang Khoa Ta, Li-Chia Lin, Syu-Ruei Pan, Yi-Chun Ni, Gangga Anuraga, Kuen-Haur Lee
Summary: Colorectal cancer is a heterogeneous disease with genetic and epigenetic changes, and molecular assessment along with biomarker use for prognosis is gaining attention. This study identifies high mRNA levels of 10 upregulated genes in CRC cells and datasets, showing correlation with worse prognosis in CRC patients. CTNNB1 is found to be a key factor in the interaction with upregulated CRC-associated genes, particularly regulating NKD2, FOXQ1, and CEMIP, which when inhibited, can suppress CRC cell growth, providing efficient biomarkers for prognosis and a new molecular interaction network for CRC.
Article
Environmental Sciences
Xianzhe Li, Ye Lu, Penghao Wen, Yan Yuan, Zhenghong Xiao, Hengwei Shi, Eryan Feng
Summary: This study aimed to explore the biological role and potential underlying mechanism of the traditional Chinese medicine matrine in colorectal cancer. The results showed that matrine can inhibit colorectal cancer development by modulating AGRN and inhibiting the activation of the Wnt/beta-catenin pathway, thereby exerting its anti-tumor effects.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Medicine, Research & Experimental
Guoqiang Yan, Shiquan Li, Meng Yue, Chenyao Li, Zhenhua Kang
Summary: The study revealed that KDM5B is abundantly expressed in colorectal cancer tissues and cells, and high KDM5B expression is associated with poor prognosis. KDM5B activates the Wnt/beta-catenin pathway by downregulating CCL14, promoting the progression of colorectal cancer.
Article
Oncology
Hu Han, Yan Li, Wan Qin, Lu Wang, Han Yin, Beibei Su, Xianglin Yuan
Summary: The study found that miR-199b-3p is significantly up-regulated in CTx-resistant colorectal cancer cells. Inhibiting miR-199b-3p can improve the sensitivity of CTx-resistant cells to CTx by regulating the Wnt/β-catenin signaling pathway. Mouse models confirmed that down-regulating miR-199b-3p can restore the inhibitory effect of CTx on tumor growth.
CANCER CELL INTERNATIONAL
(2022)
Article
Oncology
Yang Zhou, Jiang Xu, Haichang Luo, Xiangjing Meng, Ming Chen, Di Zhu
Summary: Abnormal activation of the Wnt/beta-catenin signaling pathway is closely related to tumorigenesis and immune surveillance, leading to increased resistance to immunotherapy.
Article
Biochemistry & Molecular Biology
Maria Abancens, Brian J. Harvey, Jean McBryan
Summary: Women have lower incidence and mortality rates for colorectal cancer compared to men. Estrogen may protect against colorectal cancer progression through GPER-mediated modulation of the Wnt/beta-catenin pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Hui Zhao, Shun Tang, Qiu Tao, Tianqi Ming, Jiarong Lei, Yuanjing Liang, Yuhui Peng, Minmin Wang, Maolun Liu, Han Yang, Shan Ren, Haibo Xu
Summary: Overwhelming evidence supports the role of the Wnt/beta-catenin signaling pathway in colorectal cancer (CRC) pathogenesis. Ursolic acid (UA), a pentacyclic triterpenoid found in various fruits and plants, has shown potent anticancer activity against CRC, although its mechanism of action is not fully understood. This study aimed to investigate the effects of UA on CRC and its impact on the Wnt/beta-catenin signaling pathway. The study demonstrated that UA significantly inhibited proliferation, migration, and clonality, induced apoptosis, and arrested the cell cycle in CRC cells. Moreover, UA suppressed tumor growth, improved pathological features, induced apoptosis, and arrested the cell cycle in CRC tissue, by diminishing the Wnt/beta-catenin signaling pathway. These findings suggest that UA has the potential to inhibit the malignant phenotype of CRC by attenuating the Wnt/beta-catenin signaling axis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Yan Zou, Limin Liu, Jie Meng, Meiyu Dai
Summary: This study aimed to investigate the influence and mechanism of circular RNA hsa_circ_0068464 (circ_0068464) on the development of colorectal cancer (CRC). The study found that circ_0068464 was abnormally up-regulated in CRC cells and tissues, and its knockdown inhibited CRC cell migration and proliferation while promoting apoptosis. The mechanism involved the regulation of Wnt/beta-catenin pathway activation through targeting miR-383. This study provides new insights into the molecular mechanisms underlying the occurrence and development of CRC.
Article
Cell Biology
Vikash Singh, Vonn Walter, Irina Elcheva, Yuka Imamura Kawasawa, Vladimir S. Spiegelman
Summary: In this study, the role of IGF2BP1 in the post-transcriptional regulation of Wnt/beta-catenin-induced genes in colorectal cancer (CRC) cells was investigated using iCLIP and RNA sequencing techniques. The study revealed that CRC cells exhibit a stronger dependency on IGF2BP1 expression for Wnt/beta-catenin-regulated genes compared to normal cells. iCLIP analysis showed significant changes in IGF2BP1-binding sites and enrichment of 6-mer motifs associated with IGF2BP1 binding in response to Wnt/beta-catenin signaling. Additionally, a signature of IGF2BP1-regulated genes significantly associated with colon cancer-free survival in humans was identified.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Oncology
Christopher Berlin, Felicie Cottard, Dominica Willmann, Sylvia Urban, Stephan M. Tirier, Lisa Marx, Karsten Rippe, Mark Schmitt, Valentina Petrocelli, Florian R. Greten, Stefan Fichtner-Feigl, Rebecca Kesselring, Eric Metzger, Roland Schuele
Summary: This study identifies lysine methyltransferase 9 (KMT9) as an important regulator in colorectal tumorigenesis. KMT9 alpha and KMT9 beta are overexpressed in colorectal cancer and colocalize with H4K12me1 at promoters of proliferation-related target genes. Ablation of KMT9 alpha significantly reduces colorectal tumorigenesis and prevents the growth of tumor organoids. Loss of KMT9 alpha impairs the maintenance and function of colorectal cancer stem/initiating cells and induces apoptosis specifically in this cellular compartment. These findings suggest that KMT9 could be a promising therapeutic target for colorectal cancer treatment.