Strategies for Enhancing the Yield of the Potent Insecticide Spinosad in Actinomycetes

Heterologous Expression of Spinosyn Biosynthetic Gene Cluster in Streptomyces Species Is Dependent on the Expression of Rhamnose Biosynthesis Genes

(2017) Chen Zhao et al.   JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY

Rational synthetic pathway refactoring of natural products biosynthesis in actinobacteria

(2017) Gao-Yi Tan et al.   METABOLIC ENGINEERING

Heterologous Biosynthesis of Spinosad: An Omics-Guided Large Polyketide Synthase Gene Cluster Reconstitution in Streptomyces

(2017) Gao-Yi Tan et al.   ACS Synthetic Biology

Toward Systems Metabolic Engineering of Streptomycetes for Secondary Metabolites Production

(2017) Helene Lunde Robertsen et al.   Biotechnology Journal

Identification and Engineering of Post-PKS Modification Bottlenecks for Ansamitocin P-3 Titer Improvement in Actinosynnema pretiosum subsp. pretiosum ATCC 31280

(2017) Xinjuan Ning et al.   Biotechnology Journal

High Level of Spinosad Production in the Heterologous Host Saccharopolyspora erythraea

(2016) Jun Huang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Tools of pathway reconstruction and production of economically relevant plant secondary metabolites in recombinant microorganisms

(2016) Clarissa Dziggel et al.   Biotechnology Journal

Tools of pathway reconstruction and production of economically relevant plant secondary metabolites in recombinant microorganisms

(2016) Clarissa Dziggel et al.   Biotechnology Journal

Proteomic insights into metabolic adaptation to deletion of metE in Saccharopolyspora spinosa

(2015) Qi Yang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system

(2015) Hu Zeng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Characterization of the chromosomal integration of Saccharopolyspora plasmid pCM32 and its application to improve production of spinosyn in Saccharopolyspora spinosa

(2015) Jian Chen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Increasing antibiotic production yields by favoring the biosynthesis of precursor metabolites glucose-1-phosphate and/or malonyl-CoA in Streptomyces producer strains

(2015) Daniel Zabala et al.   JOURNAL OF ANTIBIOTICS

Engineering validamycin production by tandem deletion of γ-butyrolactone receptor genes in Streptomyces hygroscopicus 5008

(2015) Gao-Yi Tan et al.   METABOLIC ENGINEERING

The structure of SpnF, a standalone enzyme that catalyzes [4 + 2] cycloaddition

(2015) Christopher D Fage et al.   Nature Chemical Biology

CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

(2015) Yaojun Tong et al.   ACS Synthetic Biology

Four-stage dissolved oxygen strategy based on multi-scale analysis for improving spinosad yield bySaccharopolyspora spinosa ATCC49460

(2015) Yun Bai et al.   Microbial Biotechnology

Chemoenzymatic Synthesis of Spinosyn A

(2014) Hak Joong Kim et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Improvement of the ability to produce spinosad in Saccharopolyspora spinosa through the acquisition of drug resistance and genome shuffling

(2014) Hang Wang et al.   ANNALS OF MICROBIOLOGY

Structural Studies of the Spinosyn Forosaminyltransferase, SpnP

(2014) Eta A. Isiorho et al.   BIOCHEMISTRY

Genome-scale metabolic network reconstruction of Saccharopolyspora spinosa for Spinosad Production improvement

(2014) Xiaoyang Wang et al.   Microbial Cell Factories

Differential proteomic profiling reveals regulatory proteins and novel links between primary metabolism and spinosad production in Saccharopolyspora spinosa

(2014) Qi Yang et al.   Microbial Cell Factories

High-throughput platform for the discovery of elicitors of silent bacterial gene clusters

(2014) M. R. Seyedsayamdost   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

Stepwise increase of spinosad production in Saccharopolyspora spinosa by metabolic engineering

(2013) Chaoyou Xue et al.   BIOCHEMICAL ENGINEERING JOURNAL

Up-regulated spinosad pathway coupling with the increased concentration of acetyl-CoA and malonyl-CoA contributed to the increase of spinosad in the presence of exogenous fatty acid

(2013) Chaoyou Xue et al.   BIOCHEMICAL ENGINEERING JOURNAL

A Comparative Metabolomics Analysis ofSaccharopolyspora spinosaWT, WH124, and LU104 Revealed Metabolic Mechanisms Correlated with Increases in Spinosad Yield

(2013) Fanglong ZHAO et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Activating secondary metabolism with stress and chemicals

(2013) Vanessa Yoon et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Chemical Perturbation of Secondary Metabolism Demonstrates Important Links to Primary Metabolism

(2012) Arryn Craney et al.   CHEMISTRY & BIOLOGY

Promotion of spinosad biosynthesis by chromosomal integration of the Vitreoscilla hemoglobin gene in Saccharopolyspora spinosa

(2012) YuShuang Luo et al.   Science China-Life Sciences

Head Lice and the Use of Spinosad

(2011) Susan C. Villegas et al.   CLINICAL THERAPEUTICS

Duplication of partial spinosyn biosynthetic gene cluster in Saccharopolyspora spinosa enhances spinosyn production

(2011) Ying Tang et al.   FEMS MICROBIOLOGY LETTERS

Genome Sequence of the Spinosyns-Producing Bacterium Saccharopolyspora spinosa NRRL 18395

(2011) Y. Pan et al.   JOURNAL OF BACTERIOLOGY

Spinosad: A new natural product for stored grain protection

(2011) Mark B. Hertlein et al.   JOURNAL OF STORED PRODUCTS RESEARCH

Enzyme-catalysed [4+2] cycloaddition is a key step in the biosynthesis of spinosyn A

(2011) Hak Joong Kim et al.   NATURE

Resistance and cross-resistance to the spinosyns – A review and analysis

(2011) Thomas C. Sparks et al.   PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

Comparative Proteomic Analysis of saccharopolyspora spinosa SP06081 and PR2 strains reveals the differentially expressed proteins correlated with the increase of spinosad yield

(2011) Yushuang Luo et al.   Proteome Science

Improvement of spinosad production by overexpression of gtt and gdh controlled by promoter PermE* in Saccharopolyspora spinosa SIPI-A2090

(2010) Hai-Xue Pan et al.   BIOTECHNOLOGY LETTERS

Streptomyces and Saccharopolyspora hosts for heterologous expression of secondary metabolite gene clusters

(2010) Richard H. Baltz   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Biosynthesis of Spinosyn inSaccharopolyspora spinosa: Synthesis of Permethylated Rhamnose and Characterization of the Functions of SpnH, SpnI, and SpnK

(2010) Hak Joong Kim et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enhanced Production of Spinosad in Saccharopolyspora spinosa by Genome Shuffling

(2009) Z. H. Jin et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Functional Characterization and Substrate Specificity of Spinosyn Rhamnosyltransferase byin VitroReconstitution of Spinosyn Biosynthetic Enzymes

(2009) Yi-Lin Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Novel mode of action of spinosad: Receptor binding studies demonstrating lack of interaction with known insecticidal target sites

(2009) Nailah Orr et al.   PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

Improvement of Saccharopolyspora spinosa and the Kinetic Analysis for Spinosad Production

(2008) Yan Liang et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

SpnH from Saccharopolyspora spinosa encodes a rhamnosyl 4′-O-methyltransferase for biosynthesis of the insecticidal macrolide, spinosyn A

(2008) Ke-xue Huang et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

In VitroCharacterization of the Enzymes Involved in TDP-d-Forosamine Biosynthesis in the Spinosyn Pathway ofSaccharopolysporaspinosa

(2008) Lin Hong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY