Article
Cell Biology
Jessica C. Hargarten, Guowu Hu, Waleed Elsegeiny, Peter R. Williamson
Summary: Macroautophagy/autophagy is a regulated cellular degradation process essential for cell survival and involved in the regulation of various cellular processes in eukaryotes. We have developed a flow cytometric assay for the quantification of intracellular SQSTM1, which offers improved sensitivity compared to conventional immunoblotting and allows for higher throughput analysis with reduced cellular material requirements. Our assay can detect changes in intracellular SQSTM1 levels following different treatments and manipulations. It utilizes readily available reagents and standard flow cytometry equipment without the need for transfection.
Article
Biochemistry & Molecular Biology
Cheong-Yong Yun, Nahyun Choi, Jae Un Lee, Eun Jung Lee, Ji Young Kim, Won Jun Choi, Sang Ho Oh, Jong-Hyuk Sung
Summary: This study demonstrated that the marliolide derivative DMF02 can induce melanosome degradation through autophagy pathway by activating Nrf2, leading to reduced melanin pigmentation. Additionally, DMF02 was effective in reducing UVB-induced hyperpigmentation and regulating melanosome degradation via suppression of p62. These findings suggest that Nrf2 activators could be promising therapeutic agents for reducing hyperpigmentation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Yinfeng Xu, Yaosen Wu, Lei Wang, Zhuo Ren, Lijiang Song, Hui Zhang, Chuying Qian, Qian Wang, Zhengfu He, Wei Wan
Summary: The study uncovered a regulatory role of autophagy in RNA metabolism, showing that autophagy-deficient cells have higher levels of 47S precursor rRNA due to accumulation of SQSTM1. This accumulation potentiates MTORC1 signaling, leading to increased rRNA transcription from rDNA and promoting protein synthesis and cell proliferation.
Article
Multidisciplinary Sciences
Min Ji Yoon, Boyoon Choi, Eun Jin Kim, Jiyeon Ohk, Chansik Yang, Yeon-Gil Choi, Jinyoung Lee, Chanhee Kang, Hyun Kyu Song, Yoon Ki Kim, Jae-Sung Woo, Yongcheol Cho, Eui-Ju Choi, Hosung Jung, Chungho Kim
Summary: The study reveals that UXT functions as an autophagy adaptor for p62-dependent aggrephagy, delaying motor neuron degeneration in a Xenopus model. Additionally, the cooperative relationship between molecular chaperones and the aggrephagy machinery is demonstrated for efficient removal of misfolded protein aggregates.
NATURE COMMUNICATIONS
(2021)
Review
Oncology
Jinlong Tang, Yuan Li, Shuli Xia, Jinfan Li, Qi Yang, Kefeng Ding, Honghe Zhang
Summary: SQSTM1/p62 is a versatile protein involved in regulating various processes in tumor cells, such as genetic stability, autophagy, and cell death, impacting tumor occurrence, development, as well as patient treatment and prognosis.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2021)
Article
Cell Biology
Heide Schatten
Summary: Selective autophagy is a targeted degradation process that eliminates specific intracellular substrates through the autophagy pathway. Blocking autophagy in cumulus granulosa cells directly affects citrate levels and thus affects oocyte maturation quality. The connection between SQSTM1 and K63-polyubiquitinated ACLY compromises citrate homeostasis and can be used as an indicator of oocyte meiotic maturation quality in cumulus granulosa cells.
Review
Cell Biology
Xiaolan Fan, Tiantian Huang, Yingdong Tong, Ziqiang Fan, Ziyue Yang, Deying Yang, Xueping Mao, Mingyao Yang
Summary: p62, also known as SQSTM1, is a crucial predictor of autophagic flux and plays a role in maintaining protein homeostasis in cells. It has the ability to resist oxidative stress, and its multiple domains allow it to interact with vital signaling pathways, influencing cell proliferation, apoptosis, and survival. Recent studies have also shown its involvement in the aging process.
AGEING RESEARCH REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Yasukazu Takanezawa, Ryohei Harada, Yoshio Shibagaki, Yui Kashiwano, Ryosuke Nakamura, Yuka Ohshiro, Shimpei Uraguchi, Masako Kiyono
Summary: p62 interacts with NEDD4 to alleviate MeHg toxicity through the formation of a complex.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Chao Zhang, Hong-Liang Zhang, Shan -Ling Liu, Jun-Mei Yang, Feng-Hou Gao
Summary: Inactive CDK2 protein is degraded through the autophagy-lysosome pathway. CDK2 degradation is mediated by the inhibition of PI3K alpha/AKT(T308). Additionally, P62/SQSTM1 binds to the inactive CDK2 protein to facilitate its entry into autophagy-lysosome degradation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Daniela Liskiewicz, Arkadiusz Liskiewicz, Mateusz Grabowski, Marta Maria Nowacka-Chmielewska, Konstancja Jablonska, Anna Wojakowska, Lukasz Marczak, Jaroslaw J. Barski, Andrzej Malecki
Summary: The study evaluated the influence of the ketogenic diet on hepatic autophagy, showing an upregulation of autophagy in animals fed with the diet. The magnitude of this effect appeared to vary depending on the diet composition. Additionally, markers of regulatory pathways linking the ketogenic diet to autophagy were measured, revealing treatment-specific effects such as an upregulation of SIRT1 and downregulation of FOXO3 and p53.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Article
Oncology
Sheng Wang, Wei Feng, Wulin Wang, Xiaoman Ye, Hao Chen, Chunzhao Yu
Summary: This study aims to increase the sensitivity of gemcitabine chemotherapy for pancreatic cancer by silencing the Girdin gene, showing that Girdin may enhance chemotherapy resistance to gemcitabine through regulating autophagy activity.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Xue Shi, Wenfeng Wu, Zhenhuan Feng, Peiyang Fan, Ruona Shi, Xiaofei Zhang
Summary: This study reveals a mechanism of regulating autophagy by demonstrating that MARCH7 inhibits autophagy through ubiquitinating ATG14, leading to reduced interactions between ATG14 and STX17 and decreased autophagy flux.
Article
Food Science & Technology
Shangyun Lu, Jiali Xu, Yang Xu, Yang Liu, Dongxing Shi, Jia Wang, Fubin Qiu
Summary: Maintaining the integrity and stability of the gut tract is crucial for overall body health. This study focused on using autophagy to balance gut homeostasis and found that glycyrol (GC), a coumarin compound from Glycyrrhiza uralensis Fisch, can activate autophagy and attenuate colon injury. By inhibiting the interaction between SQSTM1/p62 and ubiquitin-specific protease 8 (USP8), GC promotes the ubiquitination of p62 and activates autophagy. These findings suggest that GC has potential as a novel colon protector and should be further investigated.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Cell Biology
Joo-Hui Han, Keun-Woo Jang, Chang-Seon Myung
Summary: Garcinia cambogia attenuates the expression of CEBPB, a key factor in adipogenesis, and inhibits autophagic flux, potentially providing new therapeutic targets for the treatment of obesity.
Article
Biochemistry & Molecular Biology
Toshinaru Fukae, Takeshi Miyatsuka, Miwa Himuro, Yuka Wakabayashi, Hitoshi Iida, Shuhei Aoyama, Tomoya Mita, Fuki Ikeda, Hidenori Haruna, Noriyuki Takubo, Yuya Nishida, Toshiaki Shimizu, Hirotaka Watada
Summary: Autophagy plays a crucial role in intracellular quality control, while the accumulation of p62 under autophagy-deficient conditions has little effect on 0 cell function.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Jihoon Jo, Sunkyung Choi, Jooseong Oh, Sung-Gwon Lee, Song Yi Choi, Kee K. Kim, Chungoo Park
BMC BIOINFORMATICS
(2019)
Article
Biochemistry & Molecular Biology
Sunkyung Choi, Ki-Jung Kim, Seongmin Cheon, Eun-Mi Kim, Yong-An Kim, Chungoo Park, Kee K. Kim
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Polymer Science
JunJie Yu, SuJeong Lee, Sunkyung Choi, Kee K. Kim, Bokyeong Ryu, C-Yoon Kim, Cho-Rok Jung, Byoung-Hyun Min, Yuan-Zhu Xin, Su A. Park, Wandoo Kim, Donghyun Lee, JunHee Lee
Article
Cell Biology
Arnoldo Cambronero-Urena, Sunkyung Choi, Seri Choi, Kee K. Kim, Eun-Mi Kim
Summary: This study aimed to confirm the effects of certain substances on cell behavior. SG formation was not observed after treatments with the substances, and there was no change in the phosphorylation of the translation initiation factor eIF2 alpha. These findings could help determine the biological hazards of these materials.
ANIMAL CELLS AND SYSTEMS
(2021)
Article
Cell Biology
Minho Won, Sunkyung Choi, Seonghye Cheon, Eun-Mi Kim, Taeg Kyu Kwon, Jaewhan Kim, Yong-Eun Kim, Kyung-Cheol Sohn, Gang Min Hur, Kee K. Kim
Summary: The novel phenolic derivative, octyl syringate (OS), has been found to selectively induce cytotoxicity in cancer cells without causing significant toxic effects on non-transformed cells. OS induces non-apoptotic cell death in cancer cells through inhibiting autophagic flux and destabilizing lysosomal function. Furthermore, OS demonstrates promising anti-tumor effects by reducing tumor growth in mouse xenograft models.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Environmental Sciences
Seri Choi, Sunkyung Choi, Yeongsoo Choi, Namjoon Cho, Seung-Yeon Kim, Chang Hyun Lee, Han-Jin Park, Won Keun Oh, Kee K. Kim, Eun-Mi Kim
Summary: The study investigated the impact of PHMG-p on human lung tissue models under oxidative stress and viral infection, revealing that PHMG-p induced SG formation and severe lung toxicity. Evaluation of toxicity under stressful conditions is crucial for accurately predicting the potential effects of chemicals on the respiratory tract.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Sunkyung Choi, Namjoon Cho, Kee K. Kim
Summary: The underlying mechanisms of splicing regulation through non-canonical splice junction processing remain largely unknown. In this study, two splicing isoforms of RBFOX2 gene were identified, which were expressed through non-canonical and canonical splice junction processing, respectively. The cytoplasmic localization and stress granule formation of these isoforms showed differences, indicating the importance of non-canonical splice junction processing in RBFOX2 gene regulation.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sunkyung Choi, Hyo Seong Lee, Namjoon Cho, Inyoung Kim, Seongmin Cheon, Chungoo Park, Eun-Mi Kim, Wantae Kim, Kee K. Kim
Summary: Alternative splicing plays a crucial role in signaling pathways, affecting the transcriptional activity and oncogenic properties of TEAD1. RBFOX2 promotes the alternative splicing of TEAD1 by binding to a conserved sequence in the downstream intron.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Environmental Sciences
Sunkyung Choi, Eun-Mi Kim, Seung-Yeon Kim, Yeongsoo Choi, Seri Choi, Namjoon Cho, Han-Jin Park, Kee K. Kim
Summary: This study investigates the effects of respiratory particulate matter (PM) on cellular stress under virus-infected conditions. The results show that PMs, specifically PM10 and diesel PM, induce stress granule formation and cell death during respiratory syncytial virus (RSV) infection, leading to severe lung toxicity. These findings suggest the importance of studying the effects of PMs under various stress conditions to accurately predict their lung toxicity.
ENVIRONMENTAL POLLUTION
(2022)
Article
Multidisciplinary Sciences
Namjoon Cho, Jaegeon Joo, Sunkyung Choi, Bu-Gyeong Kang, Andrew J. Lee, So-Yeon Youn, Su-Hyung Park, Eun-Mi Kim, Eliezer Masliah, Yuji Ko, Sun-Shin Cha, Inkyung Jung, Kee K. Kim
Summary: Based on transcriptome analysis of substantia nigra (SN) tissues, a novel alternative splicing variant of DJ-1 called DJ-1 Delta E6 was found to be frequently detected in PD patients. Skipping exon 6 of DJ-1 was found to induce mitochondrial dysfunction and impaired antioxidant capability. This study suggests that changes in DJ-1 alternative splicing may contribute to PD progression and provide insights for studying PD etiology and potential therapeutic targets.
Review
Oncology
Sunkyung Choi, Namjoon Cho, Eun-Mi Kim, Kee K. Kim
Summary: Alternative pre-mRNA splicing plays a critical role in generating multiple mRNA and increasing protein diversity. Specific splicing isoforms have been found to be important in cellular processes, particularly in regulating cell numbers. Abnormal expression of splicing isoforms and factors, as well as disruptions in splicing caused by genetic mutations, are implicated in cancer development and progression. Alternative splicing has potential as a therapeutic target for cancer.
CANCER CELL INTERNATIONAL
(2023)
Article
Toxicology
Da-Min Jung, Eunhye Kwon, Sunkyung Choi, Kee K. Kim
Summary: Household chemical products are typically evaluated for toxicity through ingestion and inhalation, with limited information on skin absorption. However, not much is known about the acute toxic effects of these products. In this study, the effects of 1,2,4-trihydroxybenzene (THB) in human keratinocytes were examined, revealing that THB induces stress granule formation and DNA double strand breaks. Repeated exposure to THB causes apoptotic cell death. These findings suggest precautions for the use of THB-containing household chemical products.
TOXICOLOGY IN VITRO
(2023)
Review
Biochemistry & Molecular Biology
Sunkyung Choi, Namjoon Cho, Kee K. Kim
Summary: Alternative splicing plays a significant role in signal transduction pathways, enhancing or attenuating signal transmission and regulating various pathways. Aberrant splicing regulation is associated with disease onset and progression, including cancer. The understanding of splicing abnormalities in diseases can lead to new treatment approaches.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Sangsoo Lee, Haesoo Jung, Sunkyung Choi, Namjoon Cho, Eun-Mi Kim, Kee Kwang Kim
Summary: This study reveals that the expression of the METTL3 gene is regulated by alternative splicing, and intron retention inhibits the export of METTL3-IR mRNA to the cytoplasm, resulting in suppressed protein expression.
Article
Chemistry, Multidisciplinary
Namjoon Cho, Boyeong Lee, Sunkyung Choi, Jaewhan Kim, Jieun Kim, Jaehyung Yu, Kee K. Kim
Summary: The study revealed that brucite demonstrated strong antibacterial activity against Escherichia coli and Staphylococcus aureus due to its ability to maintain basicity in solution. This identified brucite as a potent antibacterial agent with a unique mechanism of action.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)