Article
Biochemistry & Molecular Biology
Wenting Xie, Dong Guo, Jieyin Li, Lei Yue, Qi Kang, Guimiao Chen, Tingwen Zhou, Han Wang, Kai Zhuang, Lige Leng, Huifang Li, Zhenyi Chen, Weiwei Gao, Jie Zhang
Summary: Alzheimer's disease is a common neurodegenerative disease with unclear underlying mechanism. The neuronal specific protein CEND1 plays an important role in Alzheimer's disease by regulating mitochondrial function and affecting cognitive function.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Oncology
Miao Wang, Ranru Wei, Guohui Li, Hai-Lian Bi, Zhaojun Jia, Mengjie Zhang, Mengyao Pang, Xiaona Li, Liming Ma, Ying Tang
Summary: The fusion protein SYNJ2BP-COX16 promotes breast cancer progression through the phosphorylation of DRP1 and subsequent induction of mitochondrial fission. SUMOylation of SYNJ2BP-COX16 recruits DRP1 to mitochondria and enhances its SUMOylation and phosphorylation, leading to mitochondrial fission.
Review
Immunology
Yewei Yang, Wenbo Lei, Lanhua Zhao, Yating Wen, Zhongyu Li
Summary: Mitochondria play crucial roles in energy production, metabolism, apoptosis, and intrinsic immunity within cells. The infection of Chlamydia, an intracellular bacterium, is closely associated with the host cell mitochondria, affecting mitochondrial fusion and fission to create a favorable environment for Chlamydia replication and growth.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cell Biology
Rong Yu, Shao-Bo Jin, Maria Ankarcrona, Urban Lendahl, Monica Nister, Jian Zhao
Summary: Drp1 exists as a mixture of self-assembly forms in mammalian cells, and its assembly state affects its binding with MIEFs and Mff. Mff binds preferentially to higher order oligomers of Drp1, while MIEFs interact with a wider range of Drp1 assembly subunits.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Srimanta Patra, Amruta Singh, Prakash P. Praharaj, Nitish K. Mohanta, Mrutyunjay Jena, Birija S. Patro, Ali Abusharha, Shankargouda Patil, Sujit K. Bhutia
Summary: SIRT1 is downregulated in oral cancer cells, leading to drug resistance. Downregulation of SIRT1 inhibits mitochondrial fission and promotes hyperfused mitochondrial networks, suppressing apoptosis. Overexpression of SIRT1 reverses mitochondrial hyperfusion and induces apoptosis. Gallic acid can counteract CDDP-mediated apoptosis inhibition by inhibiting mitochondrial hyperfusion. SIRT1 overexpression resensitizes CDDP-resistant cells to apoptosis.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Nuria Martinez-Lopez, Pamela Mattar, Miriam Toledo, Henrietta Bains, Manu Kalyani, Marie Louise Aoun, Mridul Sharma, Laura Beth J. McIntire, Leslie Gunther-Cummins, Frank P. Macaluso, Jennifer T. Aguilan, Simone Sidoli, Mathieu Bourdenx, Rajat Singh
Summary: Fasting or lipid availability stimulates mTORC2 activity, leading to phosphorylation of NDRG1 and NDRG1-CDC42-mediated mitochondrial fission and respiration.
NATURE CELL BIOLOGY
(2023)
Article
Clinical Neurology
Eduardo Benarroch
Summary: Mitochondria have multiple essential functions in cells, and their shape and dynamics play a critical role in mitochondrial function. Abnormalities in mitochondrial shape and dynamics are associated with various diseases.
Article
Biology
Yaqin Zhu, Yue Wang, Ying Li, Zhongqi Li, Wenhui Kong, Xiaoxuan Zhao, Shuting Chen, Liting Yan, Lenan Wang, Yunli Tong, Huanjie Shao
Summary: CPT1A regulates mitochondrial dynamics through succinylation of MFF to promote the growth and proliferation of ovarian cancer cells. High expression of MFF is associated with poor prognosis in ovarian cancer patients.
COMMUNICATIONS BIOLOGY
(2023)
Article
Cell Biology
Pin Chen, Yanbing Lu, Binfeng He, Tao Xie, Chaolong Yan, Tengfei Liu, Silin Wu, Yuyang Yeh, Zeyang Li, Wei Huang, Xiaobiao Zhang
Summary: The expression of Rab32 is significantly upregulated in GBM and is positively correlated with tumor malignancy and poor prognosis. Knockdown of Rab32 attenuates the migration and invasion capability of GBM and suppresses the expression of invasion-related proteins and mesenchymal transition markers. Rab32 regulates ERK1/2/Drp1-dependent mitochondrial fission and promotes migration and invasion of GBM.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Xueqiang Wang, Xing Zhang, Ke Cao, Mengqi Zeng, Xuyang Fu, Adi Zheng, Feng Zhang, Feng Gao, Xuan Zou, Hao Li, Min Li, Weiqiang Lv, Jie Xu, Jiangang Long, Weijin Zang, Jinghai Chen, Jian Ding, Jiankang Liu, Zhihui Feng
Summary: This study reveals the importance of succinate dehydrogenase complex II assembly in the development of dilated cardiomyopathy and provides insights into potential therapeutic interventions for heart diseases.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Evanthia Pangou, Olga Bielska, Lucile Guerber, Stephane Schmucker, Arantxa Agote-Aran, Taozhi Ye, Yongrong Liao, Marta Puig-Gamez, Erwan Grandgirard, Charlotte Kleiss, Yansheng Liu, Emmanuel Compe, Zhirong Zhang, Ruedi Aebersold, Romeo Ricci, Izabela Sumara
Summary: This study reveals a pathway linking mitochondrial fission to mitotic progression in mammalian cells, showing that PKD/MFF-dependent mitochondrial fission is essential for chromosome segregation and cell survival during cell division.
Article
Biochemistry & Molecular Biology
Huey-Jiun Ko, Cheng-Yu Tsai, Shean-Jaw Chiou, Yun-Ling Lai, Chi-Huei Wang, Jiin-Tsuey Cheng, Tsung-Hsien Chuang, Chi-Ying F. Huang, Aij-Lie Kwan, Joon-Khim Loh, Yi-Ren Hong
Summary: This study reveals that inducing mito-Drp1-Ser637 by PKA, rather than Drp1-Ser616, drives mitophagy to form multipolar spindles during the M-phase.
Article
Multidisciplinary Sciences
Deepshikha Ghosh, Suman Pakhira, Damayanti Das Ghosh, Susanta Roychoudhury, Sib Sankar Roy
Summary: Ovarian cancer is a leading cause of cancer-related female mortality. Ets1, a transcription factor, promotes epithelial to mesenchymal transition (EMT) and serves as a potential malignancy marker in ovarian cancer. Our study reveals that Ets1 regulates mitochondrial fission-fusion dynamics by augmenting Drp1 through direct binding at the DNM1L (DRP1) promoter. Increased Drp1 levels due to Ets1 overexpression lead to reduced mitochondrial load and compromised ATP synthase expression, resulting in a shift towards glycolysis. Additionally, inhibiting mitochondrial fission through Drp1 inhibition mitigates Ets1-associated EMT in vitro and in vivo.
Article
Food Science & Technology
Tingting Yan, Jiyang Chen, Yalin Wang, Yinuo Wang, Yuanqingzhi Zhang, Yan Zhao
Summary: This study investigated the effect of alcohol on mitochondrial function in ALDH2-deficient cells. The results showed that alcohol induced excessive mitochondrial fragmentation and impaired function, leading to increased cytotoxicity. Alcohol also caused calcium influx and activated CaMKII. Inhibition of calcium overload or CaMKII activation improved alcohol-induced mitophagy and cytotoxicity.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Endocrinology & Metabolism
Wan-Ting Dong, Li-Hong Long, Qiao Deng, Duo Liu, Jia-Lin Wang, Fang Wang, Jian-Guo Chen
Summary: This study reveals the crucial role of Drp1-dependent mitochondrial fission in neuronal energy metabolism and synaptic efficacy under chronic stress. Inhibiting or reducing Drp1 can improve depressive-like behavior, while promoting Drp1 fission increases stress susceptibility, which can be alleviated by coenzyme Q10.
Article
Oncology
Yuta Takashima, Eiki Kikuchi, Junko Kikuchi, Motofumi Suzuki, Hajime Kikuchi, Makie Maeda, Tetsuaki Shoji, Megumi Furuta, Ichiro Kinoshita, Hirotoshi Dosaka-Akita, Jun Sakakibara-Konishi, Satoshi Konno
INTERNATIONAL JOURNAL OF CANCER
(2020)
Article
Biochemistry & Molecular Biology
Tomoki Bo, Tohru Yamamori, Kumiko Yamamoto, Masaki Fujimoto, Hironobu Yasui, Osamu Inanami
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Kota Kimura, Nami Iguchi, Hitomi Nakano, Hironobu Yasui, Shingo Matsumoto, Osamu Inanami, Hiroshi Hirata
Summary: An accelerated imaging system using EPR and nitroxyl radicals was established for redox-sensitive mapping in a mouse tumor model. Sparse sampling technique was successfully applied to evaluate solution phantom and mouse xenograft models, achieving fourfold acceleration in 3D redox-sensitive mapping compared to conventional methods.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Biology
Masaki Nagane, Hironobu Yasui, Periannan Kuppusamy, Tadashi Yamashita, Osamu Inanami
Summary: Studies have shown that low-dose radiation exposure increases the risk of cardiovascular diseases, especially those related to the heart and blood vessels. The cellular senescence of vascular endothelial cells and activation of endothelial nitric oxide synthase following radiation exposure may play key roles in the pathogenesis of cardiovascular diseases.
JOURNAL OF RADIATION RESEARCH
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Tomoki Bo, Hironobu Yasui, Tohru Shiga, Yuki Shibata, Masaki Fujimoto, Motofumi Suzuki, Kei Higashikawa, Naoki Miyamoto, Osamu Inanami, Yuji Kuge
Summary: Eribulin significantly reduced tumor hypoxia by improving tumor oxygenation, enhancing the antitumor activity of radiation.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Oncology
Tatsuya Deguchi, Kenji Hosoya, Shango Kim, Yusuke Murase, Kumiko Yamamoto, Tomoki Bo, Hironobu Yasui, Osamu Inanami, Mahiro Okumura
Summary: Metformin has significant radio-sensitizing effects on CSCs by inhibiting mitochondrial function, resulting in decreased oxygen consumption, decreased mitochondrial membrane potential, and decreased ATP production in SCs. Additionally, SCs have a higher mitochondrial respiration capacity than ACs, leading to differences in sensitivity to metformin and radiation therapy.
MOLECULAR THERAPY-ONCOLYTICS
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Motofumi Suzuki, Tatsuki Katayama, Chie Suzuki, Kohei Nakajima, Yasuhiro Magata, Mikako Ogawa
Summary: The study suggests that macrophage polarization is associated with nAChR expression, and imaging of nAChRs may help identify the inflammatory status of atherosclerotic plaques.
NUCLEAR MEDICINE AND BIOLOGY
(2021)
Article
Oncology
Tomoki Bo, Sho Kobayashi, Osamu Inanami, Junichi Fujii, Osamu Nakajima, Tsunekata Ito, Hironobu Yasui
Summary: LAT1 is crucial for transporting neutral amino acids into cells and is associated with cancer malignancy. JPH203, a novel drug targeting LAT1, has shown to inhibit tumor growth and increase cancer cell sensitivity to radiation. The combination of JPH203 and radiation therapy may be a potent treatment strategy for cancer.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Chemistry, Physical
Osamu Inanami, Wakako Hiraoka, Yuto Goto, Hideo Takakura, Mikako Ogawa
Summary: Photoimmunotherapy (PIT) is a novel cancer therapy that utilizes tumor-specific monoclonal antibodies and a silicon phthalocyanine derivative to induce cell death through NIR-induced formation of IR700 anion radicals. Experimental results confirm the importance of these anion radicals in inducing the aggregation of IR700 and generating O-2(.-) under certain conditions.
Article
Neurosciences
Mayuko Hashimoto, Masaki Fujimoto, Kohtarou Konno, Ming-Liang Lee, Yui Yamada, Koya Yamashita, Chitoku Toda, Michio Tomura, Masahiko Watanabe, Osamu Inanami, Hiroshi Kitamura
Summary: This study reveals that hypothalamic USP2 regulates blood glucose levels through the ROS-AMPK-sympathetic nerve axis. Inhibition of USP2 leads to mitochondrial dysfunction and AMPK phosphorylation, resulting in increased blood glucose and norepinephrine levels.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Oncology
Masaki Fujimoto, Ritsuko Higashiyama, Hironobu Yasui, Koya Yamashita, Osamu Inanami
Summary: The study showed that inhibiting glutaminolysis enhanced radiation-induced cell death in KRAS-mutated lung cancer cell lines. Combination treatment with a glutaminolysis inhibitor and a senolytic drug could be useful for efficient radiotherapy.
TRANSLATIONAL ONCOLOGY
(2022)
Article
Chemistry, Medicinal
Takuya Kato, Ryuhei Okada, Yuto Goto, Aki Furusawa, Fuyuki Inagaki, Hiroaki Wakiyama, Hideyuki Furumoto, Dagane Daar, Baris Turkbey, Peter L. Choyke, Hideo Takakura, Osamu Inanami, Mikako Ogawa, Hisataka Kobayashi
Summary: NIR-PIT utilizes molecularly targeted antibodies with a photosensitive dye to target cancer cells, causing irreversible damage and highly immunogenic cell death through near-infrared light exposure. The use of L-NaAA can suppress acute edema, accelerate ligand release, and enhance therapeutic effects without causing side effects from unnecessary ROS production.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2021)
Article
Veterinary Sciences
Yayoi Otsuka-Yamasaki, Osamu Inanami, Haruka Shino, Reeko Sato, Masahiro Yamasaki
Summary: In this study, we investigated the effects of a CYB5R3 mutation in a Pomeranian dog family with hereditary methemoglobinemia. The mutant enzyme showed decreased heat stability and increased susceptibility to trypsin hydrolysis, suggesting a higher sensitivity to temperature-dependent denaturation compared to the wild-type form. These findings suggest that the unstable mutant enzyme properties with normal enzymatic activity may be associated with hereditary methemoglobinemia in the Pomeranian dog family.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2021)
Article
Nutrition & Dietetics
Masaki Fujimoto, Tomoki Bo, Kumiko Yamamoto, Hironobu Yasui, Tohru Yamamori, Osamu Inanami
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2020)
Article
Physiology
Mayuko Hashimoto, Natsuko Saito, Haru Ohta, Kumiko Yamamoto, Asuka Tashiro, Kosuke Nakazawa, Osamu Inanami, Hiroshi Kitamura
PHYSIOLOGICAL REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
