Article
Biochemistry & Molecular Biology
Max L. B. Gronloh, Janine J. G. Arts, Sebastian Palacios Martinez, Amerens A. van Der Veen, Lanette Kempers, Abraham C. van Steen, Joris J. T. H. Roelofs, Martijn A. Nolte, Joachim Goedhart, Jaap D. van Buul
Summary: Using a photoconvertible probe, researchers traced and identified the original endothelial transmigration hotspots, and found that these hotspots function to limit vascular leakage during transmigration events. The distribution of ICAM-1 determines the location of the transmigration hotspot.
Article
Biochemistry & Molecular Biology
Thomas Brock, Elisabeth Boudriot, Anke Klawitter, Marianne Grosser, Trang T. P. Nguyen, Sindy Giebe, Erik Klapproth, Achim Temme, Ali El-Armouche, Georg Breier
Summary: VE-cadherin enhances the adhesion of breast cancer cells to endothelial cells but does not affect their transmigration, indicating that cancer cell adhesion and incorporation are distinct processes governed by different molecular mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ricardo Huilcaman, Natalia Veliz-Olivos, Whitney Venturini, Alexandra Olate-Briones, Adriana Treuer, Claudio Valenzuela, Nelson Brown, Rodrigo Moore-Carrasco
Summary: The study found that platelets have the ability to migrate during atherosclerosis, and factors secreted by monocytes can enhance the migratory and aggregating capabilities of platelets.
Article
Medicine, Research & Experimental
Cong Zhang, Zhuowen Yang, Peng Zhou, Muxin Yu, Baorong Li, Yingmiao Liu, Jiaqi Jin, Wenhui Liu, Haijiao Jing, Jingwen Du, Jie Tian, Zhiyu Zhao, Jianxin Wang, Yinzhu Chu, ChunMei Zhang, Valerie A. Novakovic, Jialan Shi, Changjun Wu
Summary: The study found that neoadjuvant chemotherapy significantly increased the expression of PS+ BCMPs in patient plasma, which was associated with a rapid increase in procoagulant activity. Treatment with lactadherin reduced the adhesion of BCMPs and their procoagulant activity. Injection of BCMPs in mice induced a hypercoagulable state, reducing fibrinogen levels and promoting thrombus formation.
Article
Biology
Janine J. G. Arts, Eike K. Mahlandt, Max L. B. Gronloh, Lilian Schimmel, Ivar Noordstra, Emma Gordon, Abraham C. van Steen, Simon Tol, Barbara Walzog, Jos van Rijssel, Martijn A. Nolte, Marten Postma, Satya Khuon, John M. Heddleston, Eric Wait, Teng Leong Chew, Mark Winter, Eloi Montanez, Joachim Goedhart, Jaap D. van Buul
Summary: During inflammation, leukocytes rapidly transmigrate across the endothelium by choosing membrane protrusions at cell junctions, which depend on Rac1 activity. They cross the junction by migrating underneath the protruding endothelial cell, facilitating their exit from inflamed vessel walls.
Article
Microbiology
Zhaolei Zou, Juan Fang, Wanting Ma, Junyi Guo, Zhongyan Shan, Da Ma, Qiannan Hu, Liling Wen, Zhi Wang
Summary: In this study, it was found that gingipains from P. gingivalis can degrade PECAM-1 to facilitate bacterial penetration and at the same time reduce leukocyte TEM capacity. The role of gingipains in modulating vascular barrier permeability and TEM processes provides a new understanding of the distal colonization of P. gingivalis and its associated systemic diseases.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Qi Zhang, Changli Wang, Yufei Wu, Jingjing Liu, Tianyi Wang, Bing Wang
Summary: This study investigated the functions and molecular mechanism of BAP31 on the miR-206/133b cluster in colorectal cancer (CRC). The results showed that BAP31 overexpression led to a reduction in the expression of the miR-206/133b cluster, which was correlated with the transendothelial migration capability of CRC cells. The miR-206/133b cluster was found to directly regulate cell division cycle 42 (CDC42) and actin-related protein 2/3 complex subunit 5 (ARPC5) in the tight junction pathway (hsa04530), and the potential transcription regulator of the miR-206/133b cluster was identified as Homeobox D10 (HOXD10). The study further elucidated the molecular mechanisms and functional mechanisms of BAP31's regulatory role in the expression levels of the miR-206/133b cluster by inhibiting HOXD10 translocation from the cytoplasm to the nucleus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cardiac & Cardiovascular Systems
Junqi Wang, Xiaoping Chen
Summary: This review summarizes the roles of JAMs in the development of atherosclerosis and predicts future research directions.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Chiou-Mei Lee, Ming-Ling Chang, Ren-Hao Chen, Fan-Wen Chen, Jo-Chuan Liu, Shun-Li Kuo, Hsin-Hsin Peng
Summary: This study demonstrates that thrombin-activated platelets (TAPLT) can protect endothelial cells against tumor-related pro-inflammatory changes, inhibiting angiogenesis and metastasis. The protection is likely mediated by VCAM-1-mediated endothelial cell linkage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Marie Cade, Javier Munoz-Garcia, Antoine Babuty, Louis Pare, Denis Cochonneau, Karim Fekir, Mathias Chatelais, Marie-Francoise Heymann, Anna Lokajczyk, Catherine Boisson-Vidal, Dominique Heymann
Summary: This study discovered that recombinant human FVIII has direct biological activities on endothelial cells, including decreased cell adhesion, regulation of transcriptomic and protein profiles, increased vascular permeability, and enhanced monocyte adhesion. The findings highlight new functions of FVIII in regulating vascular permeability and leukocyte adhesion and migration.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Zexu Wang, Yufang Guo, Yulei Zhang, Liangquan Wu, Li Wang, Qiuqi Lin, Bing Wan
Summary: While the role and function of neutrophils are well known, the mechanisms by which they migrate from blood vessels to inflammatory sites are still not fully understood. Neutrophil migration involves complex processes and multiple steps. This review focuses on critical structures and chemotactic agents that guide the precise directional movement of neutrophils and allow them to overcome the endothelial barrier for efficient transmembrane migration.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Hematology
ShengPeng Wang, Bianbian Wang, Yue Shi, Tanja Moeller, Rebekka Stegmeyer, Boris Strilic, Ting Li, Zuyi Yuan, Changhe Wang, Nina Wettschureck, Dietmar Vestweber, Stefan Offermanns
Summary: The extravasation of leukocytes, a critical step in inflammation, requires the close interaction between leukocytes and adhesion molecules on the endothelial cells. A recent study shows that mechanical forces generated by leukocyte-induced clustering of adhesion molecule ICAM-1 synergize with fluid shear stress from flowing blood to activate the mechanosensitive cation channel PIEZO1 on the endothelial plasma membrane. This leads to downstream signaling events that result in the opening of the endothelial barrier and facilitates leukocyte diapedesis.
Article
Biochemistry & Molecular Biology
Vincent Yi-Fong Su, Shih-Hwa Chiou, Wei-Chih Chen, Wen-Kuang Yu, Huai-Hsuan Wu, Hao Chen, Kuang-Yao Yang
Summary: The study demonstrated that induced pluripotent stem cell-conditioned medium (iPSC-CM) can mitigate acute lung injury (ALI) induced by lipopolysaccharide (LPS) through promoting endogenous leukemia inhibitory factor (LIF) to inhibit neutrophil transendothelial migration and reduce the severity of sepsis-induced ALI.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cardiac & Cardiovascular Systems
Carina Mauersberger, Julia Hinterdobler, Heribert Schunkert, Thorsten Kessler, Hendrik B. Sager
Summary: This article comprehensively reviews the process of leukocyte recruitment relevant to atherosclerosis, with a focus on endothelial cells, monocytes and macrophages, neutrophils, T lymphocytes and platelets. Therapeutic options targeting leukocyte recruitment into atherosclerotic lesions, as well as potential insights into its precise mechanisms, are also highlighted.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Medicine, Research & Experimental
Bee Kee Ooi, Su Wen Phang, Phelim Voon Chen Yong, Dinesh Kumar Chellappan, Kamal Dua, Kooi-Yeong Khaw, Bey Hing Goh, Priyia Pusparajah, Wei Hsum Yap
Summary: The study aimed to investigate the role of Nrf2 in the anti-inflammatory effects of MA in atheroma pathogenesis. Opposing effects of MA on Nrf2 expression were observed in HUVECs and THP-1 macrophages. Combined effects of MA and Nrf2 inhibitor ML385 suppressed the expression of MCP-1, VCAM-1, and SR-A.
Review
Cardiac & Cardiovascular Systems
JoAnn Trial, Mark L. Entman, Katarzyna A. Cieslik
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2016)
Article
Cardiac & Cardiovascular Systems
Katarzyna A. Cieslik, Joann Trial, Mark L. Entman
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2017)
Review
Cardiac & Cardiovascular Systems
JoAnn Trial, Katarzyna A. Cieslik
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2018)
Article
Geriatrics & Gerontology
Katarzyna A. Cieslik, Rajagopal V. Sekhar, Alejandro Granillo, Anilkumar Reddy, Guillermo Medrano, Celia Pena Heredia, Mark L. Entman, Dale J. Hamilton, Shumin Li, Erin Reineke, Anisha A. Gupte, Aijun Zhang, George E. Taffet
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2018)
Article
Geriatrics & Gerontology
JoAnn Trial, Rodrigo Diaz Lankenau, Aude Angelini, Jorge E. Tovar Perez, George E. Taffet, Mark L. Entman, Katarzyna A. Cieslik
Summary: Cardiac diastolic dysfunction in aging, characterized by increased ventricular stiffness due to inflammation and interstitial fibrosis, can be prevented from worsening over time in females by the anti-inflammatory agent DCSL1. The severity of diastolic dysfunction in aging female mice, associated with unique changes in macrophage polarization, can be mitigated by treatment with DCSL1. The findings suggest that the characteristics of diastolic dysfunction in aging female mice resemble similar changes in aging women.
Article
Biochemistry & Molecular Biology
Rahul Neupane, Keith Youker, Hari Krishna Yalamanchili, Katarzyna A. Cieslik, Harry Karmouty-quintana, Ashrith Guha, Rajarajan A. Thandavarayan
Summary: This study investigates the role of CstF64 in cardiac fibrosis and finds that CstF64 is upregulated in the heart tissues and cardiac fibroblasts of heart failure patients, leading to increased fibrosis gene expression and significant shortening of the 3'UTR. Knockdown of CstF64 results in downregulation of fibrotic genes and lengthening of the 3'UTR. These findings suggest that CstF64 plays an important role in myofibroblast activation and cardiac fibrosis during heart failure.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cardiac & Cardiovascular Systems
Aude Angelini, Jesus Ortiz-Urbina, JoAnn Trial, Anilkumar K. Reddy, Anna Malovannaya, Antrix Jain, Mark L. Entman, George E. Taffet, Katarzyna A. Cieslik
Summary: The incidence of diastolic dysfunction increases with age in both humans and mice. Activating AMPK using AICAR can reduce cardiac fibrosis and improve diastolic function in female hearts, but has minimal effects in male hearts. This highlights the importance of considering sex-specific differences in pharmacological intervention.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Aude Angelini, JoAnn Trial, Alexander B. Saltzman, Anna Malovannaya, Katarzyna A. Cieslik
Summary: The study focuses on the impaired myofibroblast development in aging male heart due to stiffer extracellular matrix (ECM), and shows decreased actin polymerization, α-smooth muscle actin (α-SMA), and Kindlin-2 expressions in old male cardiac fibroblasts. Manipulation of Kindlin-2 levels reversed the defective phenotype, and inhibition of overactivated ERK1/2 rescued actin polymerization and α-SMA expression. However, old female cardiac fibroblasts retained normal mechanosensing pathway.
Meeting Abstract
Cardiac & Cardiovascular Systems
Aude Angelini, JoAnn Trial, Katarzyna A. Cieslik
CIRCULATION RESEARCH
(2020)
Review
Cell Biology
Aude Angelini, JoAnn Trial, Jesus Ortiz-Urbina, Katarzyna A. Cieslik
AGEING RESEARCH REVIEWS
(2020)
Article
Cardiac & Cardiovascular Systems
JoAnn Trial, Celia Pena Heredia, George E. Taffet, Mark L. Entman, Katarzyna A. Cieslik
BASIC RESEARCH IN CARDIOLOGY
(2017)
Meeting Abstract
Geriatrics & Gerontology
S. Karim, K. A. Cieslik, T. Pham, C. Pena Heredia, P. A. Taffet, M. L. Entman, G. E. Taffet
JOURNAL OF THE AMERICAN GERIATRICS SOCIETY
(2017)
