Article
Biophysics
Yuanyuan Wang, Dexiang Feng, Xianwen Kan
Summary: In this study, a facile strategy for the design of highly efficient electrochemiluminescence (ECL) sensor was proposed for the sensitive and accurate detection of prostate-specific antigen (PSA). The integration of carboxylated graphitic carbon nitride (g-C3N4) nanosheet (CCN) and tris (2, 2'-Bipyridyl) ruthenium (II) (Ru(bpy)32+) encapsulated in silica nanospheres (RuSi NPs) allowed for a shortened electron-transfer path and increased resonance energy transfer (RET) efficiency. The ECL signal was remarkably decreased through the introduction of aptamer, PSA, and ferroceneboronic acid (FcBA), enabling the ultrasensitive detection of PSA with high selectivity.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yu-Ting Yang, Jia-Li Liu, Man-Fei Sun, Ruo Yuan, Ya-Qin Chai
Summary: A novel electrochemiluminescence biosensor based on MnS:CdS@ZnS QDs was developed for ultrasensitive detection of miRNA let-7a. The biosensor exhibited high stability and excellent sensitivity, with a detection limit as low as 4.1 aM.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Shuo Meng, Dongmiao Qin, Yusheng Wu, Guichun Mo, Xiaohua Jiang, Biyang Deng
Summary: Researchers prepared a composite material MnCO3/poly(diallyl dimethyl ammonium chloride) (PDDA)/Ag with excellent electrochemiluminescence (ECL) performance and high biocompatibility by adding MnCO3 and PDDA to silver nanoparticles (AgNPs). MnCO3 and Au@SiO(2)NPs were used as ECL donors and acceptors, respectively, establishing an effective ECL-resonance energy transfer (RET) sensing platform. The system showed good response to the concentration of A beta(42) in serum samples.
Article
Biophysics
Jie Liu, Jie Chen, Yue Zhang, Linlei Liu, Yuzhuo Guo, Ruo Yuan, Yaqin Chai
Summary: In this study, selenium and nitrogen co-doped carbon dots (SeN-CDs) were synthesized as a new emitter with highly efficient electrochemiluminescence (ECL) and excellent biocompatibility for sensitive detection of microRNA-221 (miRNA-221). The SeN-CDs exhibited superior ECL performance compared to N-doped CDs, with selenium playing a key role in enhancing the ECL efficiency. The biosensor constructed using the SeN-CDs and a T7 Exo-assisted double cycle amplification strategy achieved ultrasensitive detection of miRNA-221, with a low detection limit of 2.3 aM. This work provides a novel method to enhance the ECL performance of carbon dots for the development of sensitive biosensing platforms for biomarker detection and disease diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Zhiyuan Cao, Chuyue Li, Yufei Shu, Meiyi Zhu, Bin Su, Haiyan Qin, Xiaogang Peng
Summary: Using CdSe/CdS/ZnS core/shell/shell quantum dots as a model system, time- and potential-resolved spectroelectrochemical measurements were successfully applied to study the mechanisms and kinetics of electrochemiluminescence (ECL) generation. The rate constant of electron injection into a negatively charged quantum dot increased with increasing potential, and the key intermediate for ECL generation was found to be structurally stable and possess slow deionization channels.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Weiwei Cao, Ruo Yuan, Haijun Wang
Summary: By introducing high-density N vacancy into the structure of 3D g-C3N4, multipath ECL improvement can be conveniently achieved, effectively solving the disadvantages of limited luminous efficiency, low content, low reactivity, and instability of dissolved O2.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wanlu Wang, Yuanyuan Wang, Xianwen Kan
Summary: In this study, a novel sandwich ECL sensor based on a dual suppression-based signal amplification strategy was proposed for ultrasensitive detection of IgG. By integrating ECL-RET and BCP techniques, an efficient dual suppressive ECL platform was achieved. The method showed high sensitivity and a wide linear range, which may have promising applications in the development of highly efficient ECL systems for clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Dongmiao Qin, Shuo Meng, Yusheng Wu, Zhi Luo, Biyang Deng
Summary: An efficient ECL-RET method was proposed by combining luminescent materials into a composite, which greatly improved RET efficiency and ECL signal. An ECL immunosensor for sensitive detection of PCT was constructed by combining the obtained composite with sandwich immunoreaction. The biosensor exhibited high sensitivity, good selectivity, and stability, making it suitable for biological analysis and clinical diagnosis.
Article
Chemistry, Analytical
Yu-Ting Yang, Yu-Zhuo Guo, Zhao-Chen Shen, Jia-Li Liu, Ruo Yuan, Ya-Qin Chai
Summary: In this study, novel alloyed silver gold sulfur quantum dots (AgAuSQDs) were successfully prepared for ultrasensitive detection of microRNA-222 (miRNA-222). The AgAuS QDs showed excellent electrochemiluminescence (ECL) emission efficiency at 707 nm, benefiting from abundant surface defects and narrow bandgaps due to Au incorporation. An improved localized catalytic hairpin self-assembly (L-CHA) system was developed to enhance the reaction speed and address the limitations of traditional CHA systems. Using AgAuS QDs as an ECL emitter and the improved L-CHA system as a signal amplification strategy, a highly sensitive biosensor with a detection limit of 10.5 aM for miRNA-222 was successfully developed.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jicui Hu, Yue Zhang, Yaqin Chai, Ruo Yuan
Summary: A novel Ru(II)-based nanocomposite was developed for ultrasensitive detection of MTB DNA fragment, showing a low detection limit of 1.4 aM. The ECL signal was controlled by coreaction and quenching effects, providing important application value in the detection of biomarkers in clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Engineering, Environmental
Mengqi Pan, Weiwei Cao, Ruo Yuan, Haijun Wang
Summary: In this study, lamellar nanostructures (LNs) of [-TPE-(COOH)4-Al-]nLNs were synthesized through the coordination between Al3+ and carboxyl groups of TPE-(COOH)4. The obtained [-TPE-(COOH)4-Al-]nLNs showed excellent aggregation-induced electrochemiluminescence (AIECL) and higher electrochemiluminescence (ECL) efficiency compared to TPE-(COOH)4. They also demonstrated better cost and biocompatibility compared to coordination aggregation structures with heavy metal or rare metal ions. Furthermore, the [-TPE-(COOH)4-Al-]nLNs were used as a luminescent material to fabricate an ultrasensitive ECL biosensor for miRNA-141 detection using the cascaded strand displacement amplification (CSDA) strategy. The biosensor exhibited excellent detection performance with a wide linear range and low detection limit. This study provides a convenient and effective AIECL strategy for TPE derivatives and expands the application of ECL technology in ultra-sensitive biochemical detection.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Yu-Zhuo Guo, Jia-Li Liu, Yi-Fei Chen, Ya-Qin Chai, Zhao-Hui Li, Ruo Yuan
Summary: By utilizing boron and nitrogen-codoped carbon dots as highly efficient ECL emitters, a novel ternary ECL system was prepared for ultrasensitive detection of HBV-DNA. The BN-CDs showed superior ECL efficiency compared to both nondoped and single-doped CDs. Combining the Exo III-induced DNA amplification strategy, an ECL biosensor was developed for the ultrasensitive detection of HBV-DNA at extremely low concentrations.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Chaonan Yang, Ya Tian, Baoying Wang, Qingfu Guo, Guangming Nie
Summary: A novel "signal-on" molecular imprinting-aptamer electrochemiluminescence (ECL) platform has been developed for ultrasensitive bioanalysis of thrombin (TB) using SiO2 loaded with Ru(bpy)32+/polyetherimide-graphene oxide (RuNP/PEI-GO) nanocomposite as signal labels. The platform combines molecular imprinting technology and "signal-on" ECL sensing strategy, providing high sensitivity, signal strength, and stability for TB detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Yi Zhang, Yuanyuan Cui, Mengmeng Sun, Tanke Wang, Tao Liu, Xianxiang Dai, Ping Zou, Ying Zhao, Xianxiang Wang, Yanying Wang, Man Zhou, Gehong Su, Chun Wu, Huadong Yin, Hanbing Rao, Zhiwei Lu
Summary: A novel, portable, and smartphone-based molecularly imprinted polymer electrochemiluminescence (MIP-ECL) sensing platform was constructed for sensitive and selective determination of furosemide (FSM). The platform utilized MoSe2/BC nanocomposites, Luc, CdS QDs, and MIP to enhance the sensitivity and specificity of the system based on ECL-RET mechanism. Deep learning enabled batch processing of ECL signals. This method showed outstanding performance in terms of detection range and limit, and allowed real-time monitoring of biochemical analytes.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Nanoscience & Nanotechnology
Jiajia Li, Wenjing Lai, Chaoyun Ma, Chulei Zhao, Pengli Li, Mingzhe Jiang, Min Wang, Siyu Chen, Chenglin Hong
Summary: In this study, a novel double-quenching electrochemical luminescence (ECL) strategy based on the double-quenching effect of Ru(bpy)32+-functionalized three-dimensional metal-organic framework (MOF) by MnO2/ PDA was proposed. An ECL immunosensor for ultrasensitive detection of alphafetoprotein (AFP) was designed. This work demonstrates the detection and application of a signal-quenched ECL immunosensor and ECL immunoassay based on a luminescence-functionalized MOF system for biomarker detection. It indicates a great prospect in the analysis of other biomarkers and clinical analysis.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Xi Zhang, Junlun Zhu, Zhen Wu, Wei Wen, Xiuhua Zhang, Shengfu Wang
Summary: An electrochemical sensor based on gold nano-particles and COFs was developed for the detection of BPA, showing high sensitivity and satisfactory recovery in real water samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Xi Chen, Wen-Tao Wu, Yu-Ting Jiao, Yi-Ran Kang, Xin-Wei Zhang, Wei-Hua Huang
Summary: Intracellular H2S is crucial for regulating cell metabolism, but its quantification is hindered by the limited sensing materials and sensor passivation. In this study, we functionalized conductive nanowires with MoS2 and quercetin to develop single nanowire sensors with excellent electrocatalytic and anti-poisoning performance, enabling accurate quantification of H2S within single cells.
CHEMICAL COMMUNICATIONS
(2023)
Review
Chemistry, Multidisciplinary
Yao Xiao, Chengyi Xiong, Miao-Miao Chen, Shengfu Wang, Lei Fu, Xiuhua Zhang
Summary: With the development of two-dimensional materials, transition metal dichalcogenides (TMDs) have become popular model materials for both fundamental sciences and practical applications. This review provides a comprehensive overview of the structure modulation of TMDs, including various modulated structures and their characteristics. It also summarizes the recent progress in modulating methods, mechanisms, properties, and applications of modulated TMD structures, as well as the challenges and prospects in this field.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Chemistry, Physical
Wenbo Li, Duoling Cao, Xiaorui Zhu, Zhiguang Guo, Li Wan, Xu Zhang, Xiuhua Zhang, Yuebin Li, Xiaoming Ren, Xianbao Wang, Dominik Eder, Shimin Wang
Summary: This study demonstrates a facile approach of optimizing the SnO2-ETL and CsPbI3 film to construct efficient carbon CE-based CsPbI3 solar cells, resulting in a relatively high power conversion efficiency of 12.85%.
ACS APPLIED ENERGY MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Lebao Mao, Yao Xiao, Hao Liu, Xiuhua Zhang, Shengfu Wang, Wei-Hua Huang, Miao-Miao Chen
Summary: Hydrolysis-resistant CsPbBr3/reduced graphene oxide nanoscrolls (CsPbBr3/rGO NSs) with excellent optical properties and stability were obtained through a solvent-assisted self-rolling process. The CsPbBr3 QDs embedded in rGO nanosheets prevent water infiltration and maintain superb optical properties. The CsPbBr3/rGO NSs exhibit enhanced anode photocurrent response and demonstrate high sensitivity and anti-interference ability in molecular imprinted PEC sensors for mycotoxin detection.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Applied
Qian Wang, Chengyi Xiong, Jingwen Li, Qianchun Deng, Xiuhua Zhang, Shengfu Wang, Miao-Miao Chen
Summary: In this study, methylamine perovskite quantum dots (MP QDs) encapsulated by ZIF-8 metal-organic frameworks (MP QDs@ZIF-8) were prepared for the detection of aflatoxin B1 (AFB1). The constructed electrochemiluminescence (ECL) sensor showed good selectivity and ultra-sensitivity, with a wide linear range for AFB1 quantification. The satisfactory recoveries in corn samples demonstrated the reliable practicability of the proposed sensor for AFB1 assay. This work provided a novel pathway for designing high-performance ECL sensing platforms in food safety.
Article
Engineering, Mechanical
Huan Yan, Peitang Wei, Lihong Su, Huaiju Liu, Dong Wei, Xiuhua Zhang, Guanyu Deng
Summary: In this study, the rolling-sliding contact fatigue (RSCF) failures of AISI 9310 gear steel were thoroughly analyzed. The fatigue life, microstructure and crystallographic orientation evolution, and microhardness and residual stress distributions were characterized. The RSCF life of AISI 9310 steel roller decreased with increasing maximum contact pressure, but could be improved by shot peening due to enhanced microhardness and compressive residual stress. Main fatigue damage near the roller surface included inclined cracks, micro-pitting, spallation, and plastic deformation. The RSCF process also resulted in preferred crystallographic orientations.
INTERNATIONAL JOURNAL OF FATIGUE
(2023)
Article
Engineering, Multidisciplinary
Xiuhua Zhang, He Ke, Mingxin Chi, Ruochen Wang
Summary: In this study, the impact of paper straw board (PSB) on the seismic performance of cold-formed thin-walled steel composite walls (CFSCWs) was investigated, and the experimental results of CFSCW with PSB cladding were compared with those of CFSCW with other wall panel cladding. The findings suggest that PSB can effectively enhance the shear bearing capacity of the composite wall, and its lightweight nature makes CFSCW with PSB cladding easy to construct. Finite element analysis was conducted using ANSYS software to simulate CFSCW with one-sided PSB cladding and analyze the relevant parameters affecting seismic performance. The results indicate that reducing stud spacing, peripheral self-tapping screw spacing, increasing wall panel and steel skeleton edge stud section thickness can effectively improve shear bearing capacity and lateral stiffness within a certain range. Additionally, decreasing the wall height-to-width ratio improves lateral stiffness but decreases unit width shear bearing capacity.
JOURNAL OF THE CHINESE INSTITUTE OF ENGINEERS
(2023)
Article
Nanoscience & Nanotechnology
Weijian Wang, Gang Yu, Lebao Mao, Sanam Attique, Zhiwei Si, Qing Yang, Yongqiang Zhang, Guiqiu Huang, Haiyan Zhang, Ximing Yan, Shengfu Wang, Xiuhua Zhang
Summary: Researchers have designed perovskite solar cells with mesoporous antireflection coatings, which significantly improve the photocurrent density of the cells. The study offers a fresh approach to the development of highly efficient perovskite and perovskite/silicon tandem solar cells.
ADVANCED ELECTRONIC MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Jinrong Liu, Yongzhong Jiang, Wei Wen, Xiuhua Zhang, Zhen Wu, Shengfu Wang
Summary: A novel and universal single-particle collision electrochemistry (SPCE) biosensor based on the collision and oxidation of single silver nanoparticles (Ag NPs) was proposed for sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The biosensor showed enhanced detection sensitivity and practicability of SPCE in biosensing by increasing the collision frequency of Ag NPs on polysulfide-functionalized gold ultra-microelectrodes (Ps-Au UMEs). Additionally, the biosensor achieved successful detection of SARS-CoV-2 in nasopharyngeal swab samples, demonstrating its potential for clinically relevant diagnosis.
Review
Chemistry, Analytical
Yi-Ke Wang, Yi-Ru Bao, Ying-Xue Liang, Yi-Jing Chen, Wei-Hua Huang, Min Xie
Summary: Exosomes, small extracellular vesicles that can carry abundant information for intercellular communication, have attracted much attention for their important roles in diseases. Microfluidic chips have shown superior performance in capturing and analyzing exosomes. This review discusses recent advances in the use of microfluidic systems for isolating and identifying exosomes, as well as developments in quantitative analysis, molecular analysis, and on-chip engineering of exosomes. The prospects of high-performance and comprehensive microfluidic platforms for exosome-related research are also proposed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Chen-Xi Bi, Kai-Qi Jin, Jing Yan, Yu Qin, Feng Hong, Wei-Hua Huang, Yan-Ling Liu
Summary: Researchers have developed a stretchable electrochemical sensor by electrospinning aligned and elastic polyurethane nanofibers on a PDMS film and modifying it with a conductive polymer. The sensor can guide the oriented growth of smooth muscle cells and maintain their contractile phenotype, while exhibiting good electrochemical sensing performance and stability under mechanical deformation. By culturing cells on the sensor surface, the oriented growth of smooth muscle cells and real-time monitoring of stretch-induced H2O2 release can be achieved. This allows for quantitative monitoring of changes in H2O2 levels released by smooth muscle cells under hypertensive conditions and intervention with the natural product resveratrol, providing insights into vascular-related diseases and pharmaceutical intervention mechanisms.