Article
Chemistry, Analytical
Yu-Ting Yang, Jia-Li Liu, Man-Fei Sun, Ruo Yuan, Ya-Qin Chai
Summary: A novel electrochemiluminescence biosensor based on MnS:CdS@ZnS QDs was developed for ultrasensitive detection of miRNA let-7a. The biosensor exhibited high stability and excellent sensitivity, with a detection limit as low as 4.1 aM.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Duo-Duo Chai, Ying Zhuo, Ting-Ting Tu, Hong-Ling Li, Ruo Yuan, Sha-Ping Wei
Summary: In this study, Ag@pyrenecarboxaldehyde nanocapsules were used as emitters to construct an ultrasensitive ECL biosensor for the detection of APE1 activity. The biosensor exhibited an exceptional linear working range and a low limit of detection, providing a new approach for biomolecule detection and early disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jie Zhao, Chuan-Xiang Chen, Jia-Wan Zhu, Hui-Long Zong, Yong-Hong Hu, Yin-Zhu Wang
Summary: A new ECL platform was constructed for detecting the prostate cancer marker miRNA-141, providing a potential method for the detection of low-concentration nucleic acids with high sensitivity and a wide linear range.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Jie Liu, Jie Chen, Yue Zhang, Linlei Liu, Yuzhuo Guo, Ruo Yuan, Yaqin Chai
Summary: In this study, selenium and nitrogen co-doped carbon dots (SeN-CDs) were synthesized as a new emitter with highly efficient electrochemiluminescence (ECL) and excellent biocompatibility for sensitive detection of microRNA-221 (miRNA-221). The SeN-CDs exhibited superior ECL performance compared to N-doped CDs, with selenium playing a key role in enhancing the ECL efficiency. The biosensor constructed using the SeN-CDs and a T7 Exo-assisted double cycle amplification strategy achieved ultrasensitive detection of miRNA-221, with a low detection limit of 2.3 aM. This work provides a novel method to enhance the ECL performance of carbon dots for the development of sensitive biosensing platforms for biomarker detection and disease diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Jin-Ling Zhang, Yang Yang, Wen-Bin Liang, Li-Ying Yao, Ruo Yuan, Dong-Rong Xiao
Summary: A novel pyrene-based sp(2) carbon-conjugated covalent organic framework nanosheet was constructed, exhibiting strong and stable electrochemiluminescence emission for the first time. The Py-sp(2)c-CON showed higher ECL intensity and efficiency compared to other materials, and when combined with Bu4NPF6, the ECL signal was further amplified, allowing for the detection of microRNA-21 with broad linear response range and low detection limit. Overall, this work demonstrates the potential of sp(2) carbon COFs as high-performance ECL emitters with expanded applications in biosensing.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Mohammad Ali Kamyabi, Mohsen Moharramnezhad
Summary: An innovative enzyme free electrochemiluminescence chlorpyrifos sensor was developed using a ternary nanocomposite for signal enhancement. The modified electrode showed superior electrocatalytic performance and successfully allowed for trace analysis of chlorpyrifos with wide linear range and low detection limit.
Article
Chemistry, Analytical
Yu-Ting Yang, Yu-Zhuo Guo, Zhao-Chen Shen, Jia-Li Liu, Ruo Yuan, Ya-Qin Chai
Summary: In this study, novel alloyed silver gold sulfur quantum dots (AgAuSQDs) were successfully prepared for ultrasensitive detection of microRNA-222 (miRNA-222). The AgAuS QDs showed excellent electrochemiluminescence (ECL) emission efficiency at 707 nm, benefiting from abundant surface defects and narrow bandgaps due to Au incorporation. An improved localized catalytic hairpin self-assembly (L-CHA) system was developed to enhance the reaction speed and address the limitations of traditional CHA systems. Using AgAuS QDs as an ECL emitter and the improved L-CHA system as a signal amplification strategy, a highly sensitive biosensor with a detection limit of 10.5 aM for miRNA-222 was successfully developed.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jie Liu, Yue Zhang, Ruo Yuan, Yaqin Chai
Summary: In this study, fluorine-nitrogen co-doped carbon dots (FNCDs) were used as a novel electrochemiluminescence (ECL) emitter for ultrasensitive detection of human immunodeficiency virus (HIV) DNA fragment. The doping of fluorine and nitrogen in carbon dots (CDs) improved the ECL intensity and stability of FNCDs by forming hydrogen bonds and changing the charge distribution. The ECL biosensor fabricated using exonuclease III (Exo III)-assisted amplification achieved ultrasensitive detection of HIV-DNA fragment.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Xiang Hu, Ying Wang, Xiong Zuping, Pei Song, Ai-Jun Wang, Zhaosheng Qian, Pei-Xin Yuan, Tiejun Zhao, Jiu-Ju Feng
Summary: In this study, an aggregation-enhanced PEC photosensitizer PPIX-[BMIm] was prepared by electrostatic bonding, and its PEC performance in a binary solvent of water and DMSO was studied. A label-free PEC cytosensor was built using PPIX-[BMIm], which showed a wide linear range and low limit of detection for detecting leukemia cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Duo-Duo Chai, Ying Zhuo, Ting-Ting Tu, Hong-Ling Li, Ruo Yuan, Sha-Ping Wei
Summary: In this study, Ag@pyrenecarboxaldehyde nanocapsules were prepared and used as emitters to construct an ultrasensitive ECL biosensor for the detection of APE1 activity. Ag nanoparticles on the surface of nanocapsules could promote coreactant S2O82- to generate reactive intermediates, leading to a strong ECL response. The APE1-triggered 3D DNA machine was employed to convert trace target into mimic targets, which were positively correlated with APE1 activity.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jicui Hu, Yue Zhang, Yaqin Chai, Ruo Yuan
Summary: A novel Ru(II)-based nanocomposite was developed for ultrasensitive detection of MTB DNA fragment, showing a low detection limit of 1.4 aM. The ECL signal was controlled by coreaction and quenching effects, providing important application value in the detection of biomarkers in clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Mei-Ling Lu, Wei Huang, Shuzhen Gao, Jia-Ling Zhang, Wen-Bin Liang, Yan Li, Ruo Yuan, Dong-Rong Xiao
Summary: In this study, a pyrene-based hydrogen-bonded organic framework (Py-HOF) with remarkable electrochemiluminescence (ECL) performance was prepared. The enhanced ECL emission of Py-HOF was attributed to the assembly of H4TBAPy into J-aggregates through hydrogen bonding and the high porosity of Py-HOF. Py-HOF was successfully employed as a probe for an ultrasensitive ECL sensor, demonstrating satisfactory detection performance.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Zhuoxin Ye, Yibing Liu, Meichen Pan, Xiuli Tao, Yuxuan Chen, Pinyi Ma, Ying Zhuo, Daqian Song
Summary: In this study, low-toxic quaternary AgInZnS quantum dots were synthesized and used as novel anodic electrochemiluminescence (ECL) emitters. These QDs exhibited strong and stable ECL emission, a low excitation potential, and high ECL efficiency. An on-off-on ECL biosensor for detecting microRNA-141 was designed based on a dual isothermal enzyme-free strand displacement reaction, achieving cyclic amplification of the target and ECL signal, as well as a switch function. The ECL biosensor showed a wide linear range and low detection limit, making it a promising tool for rapid and accurate diagnosis of clinical diseases.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Yu-Yi Zhou, Guan-Feng Li, Rong-Xian Ma, Yu Lin, Jia-Wen Wu, Ye-Yu Wu, Jun Yan, Shao-Gang Liu, Xue-Cai Tan, Ke-Jing Huang
Summary: One highly attractive research direction in the ECL field is the regulation and improvement of ECL efficiency, which is achievable with the use of quantum dots (QDs) as ECL materials. The study presents the synthesis of MoS2 NSs@QDs, an ECL emitter, and investigates its ECL mechanism. It also develops an innovative ECL sensor for the precise and ultrasensitive detection of target miRNA-199a.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Sakda Jampasa, Nattaya Ngamrojanavanich, Sirirat Rengpipat, Orawon Chailapakul, Kurt Kalcher, Sudkate Chaiyo
Summary: An ultrasensitive ECL sensor based on nitrogen-decorated carbon dots was developed for Listeria monocytogenes detection, showing high sensitivity and specificity for food products. The sensor construction using green microwave-assisted methodology holds promising potential for effectively controlling the spread of foodborne diseases.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Chemistry, Analytical
Jingyu Hu, Yuanqiang Sun, Aaron Albert Aryee, Lingbo Qu, Ke Zhang, Zhaohui Li
Summary: This review provides an overview of both conventional and emerging mechanisms used in carbon dot (CD) based fluorescent probe designs. It discusses the applications of CDs in chemosensing, biosensing, and bioimaging, and also discusses several other mechanisms that have potential but have not been widely applied in CD-based sensing systems.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Zhijun Li, Qiannan Li, Yanan Wu, Kun Yuan, Mingqing Shi, Yiwei Li, Hong-Min Meng, Zhaohui Li
Summary: Efficient monitoring of microRNA is highly important for cancer research. In this study, a multivalent self-assembled DNA nanowire was designed for fast quantification of intracellular target miRNAs. The experimental results showed that the DNA nanowire probe successfully and quickly monitored miRNA-21 in living cells and in vivo. This developed strategy may serve as a general platform for detecting targets in living cells and has great potential for biological and diagnostic research.
Article
Chemistry, Analytical
Juan Chen, Mingyue Xie, Mingqing Shi, Kun Yuan, Yanan Wu, Hong-Min Meng, Lingbo Qu, Zhaohui Li
Summary: In this study, an accelerated DNA nanoprobe was developed for fast, in situ monitoring of miRNA in exosomes. The nanoprobe not only detects miRNA, but also distinguishes tumor exosomes from those derived from normal cells, and can be used to study exosome biological functions.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Dongfang Liu, Jinlu Tang, Hui Xu, Kun Yuan, Aaron Albert Aryee, Cuijie Zhang, Hongmin Meng, Lingbo Qu, Zhaohui Li
Summary: In this study, a split-aptamer mediated regenerable temperature-sensitive electrochemical biosensor was developed for the detection of exosomes. The sensor allowed sensitive and specific analysis of target exosomes and could be quickly regenerated for reuse.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Yue Xiao, Xiaohui Yin, Pengjuan Sun, Yuanqiang Sun, Lingbo Qu, Zhaohui Li
Summary: The development of new carbon dots for fluorescence-based cancer diagnosis is promising, with advantageous properties and the ability to accurately diagnose cancer cells based on changes in their microenvironment.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Analytical
Lingqi Kong, Zeshuai Han, Mingzhou Zhao, Xiaolong Zhang, Ying Zhuo, Yaqin Chai, Zhaohui Li, Ruo Yuan
Summary: This study presents the development of an ultrasensitive and versatile electrochemical biosensor for the detection of cancer-related biomarkers (miRNA-21 and GSH) through target-controlled capture and release of signal probe-loaded DNA nanotubes. The biosensor demonstrated high sensitivity and the ability to detect different types of biomarkers, reducing false-positive responses and improving the accuracy and precision of early cancer diagnosis. The proposed biosensor also allowed for regenerative analysis of targets, enhancing analytical efficiency.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Shuo Guo, Yuanqiang Sun, Zhaofan Wu, Ran Yang, Lingbo Qu, Zhaohui Li
Summary: In this study, a new type of fluorescent carbon dots (CDs) was developed for simultaneous monitoring of mitochondrial viscosity and membrane potential in living cells. This provides a promising fluorescent probe for in-depth studies on mitochondria-related biology.
Article
Chemistry, Analytical
Qiannan Li, Yufei Wang, Qianqian Zhu, Haifang Liu, Jianbo Liu, Hong-Min Meng, Zhaohui Li
Summary: A novel dual-mode sensing system based on molybdophosphoric heteropoly acid (PMA) and fluorescent microspheres (FMs) was developed for monitoring alkaline phosphatase (ALP) activity. The system utilized the catalytic hydrolysis of l-ascorbic acid-2-phosphate (AAP) by ALP to generate a colorimetric signal and reduce PMA to phosphorus molybdenum blue (PMB). Additionally, the fluorescence of FMs was quenched through the inner-filter effect, enabling simultaneous fluorescence detection. The system showed high sensitivity with a limit of detection of 0.27 U L-1 and 0.11 U L-1, and demonstrated good analytical performance in biological samples. A portable test kit for visual detection of ALP in serum samples was also developed using a smartphone as a visual detection platform.
Article
Engineering, Environmental
Jie Zhang, Yingying Li, Jingyu Hu, Yuanqiang Sun, Ran Yang, Zhaohui Li, Lingbo Qu
Summary: This study developed a rapid, visual, and on-site detection method for phosgene using sensitive and selective carbon dots as detection materials. The detection of phosgene was achieved by modifying the color and fluorescence of the carbon dots. Additionally, a portable droplet system was developed for on-site detection and long-term visualization of phosgene.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Zengqiang Liang, Yuanqiang Sun, Huajin Zeng, Haimei Qin, Ran Yang, Lingbo Qu, Ke Zhang, Zhaohui Li
Summary: Sensing systems based on cholinesterase and carboxylesterase combined with various transduction technologies have been widely used for pesticide screening due to their simplicity, speed, and suitability for on-site analysis. However, current enzymatic sensors face a challenge in the broad-spectrum and specific screening of pyrethroids. Human serum albumin (HSA), a multifunctional protein, has been found to have a catalytic activity in hydrolyzing the coumarin lactone bond, which enables the development of a specific and broad-spectrum pyrethroids sensing system.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yaqing Yang, Simin Wei, Jialin Wang, Junjie Li, Jinlu Tang, Albert Aryee Aaron, Qiyong Cai, Ningning Wang, Zhaohui Li
Summary: In this study, a NO2--activated ratiometric upconversion luminescence (UCL) nanosensor was designed for NO2- detection. The nanosensor utilized the inner filter effect (IFE) between NO2--sensitive carbon dots (CDs) and upconversion nanoparticles (UCNPs) to achieve detection. By taking advantage of NIR excitation and ratiometric detection signal, the nanosensor effectively eliminated autofluorescence and successfully detected NO2- quantitatively in actual samples. This nanosensor provides a simple and sensitive sensing strategy for NO2- detection and analysis, which can be utilized in food safety.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Qiyong Cai, Shuiling Jin, Hong Zong, Lu Pei, Ke Cao, Lingbo Qu, Zhaohui Li
Summary: This study developed a quadruplex ultrasensitive immunoassay for simultaneous assessment of four reproductive hormone proteins in various biofluid samples. The assay exhibited good performance and consistency with clinical test results, allowing for rapid and accurate diagnosis of reproductive endocrine disorders.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Junli Wang, Yuanqiang Sun, Jingyu Hu, Yifei Guo, Lingbo Qu, Zhaohui Li
Summary: This study developed a novel near-infrared carbon dots with dual-report function that accurately identifies the polarity and spatial characteristics of lipid droplets in hepatocellular carcinoma (HCC). The carbon dots can report the number, size, aggregation degree, and polarity variations of lipid droplets using fluorescence imaging technology, enabling high-contrast fluorescence diagnosis of HCC and evaluation of anticancer drug efficacy.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Spectroscopy
Sen Li, Jingyu Hu, Aaron Albert Aryee, Yuanqiang Sun, Zhaohui Li
Summary: Disposable medical masks are widely used to prevent respiratory infections, but their improper disposal poses a threat to the environment and human health. This study proposes a hydrothermal method to disinfect and transform waste medical masks into carbon dots with blue-emissive fluorescence, providing a sustainable and value-added solution.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
