Advancements in microfluidic technologies for isolation and early detection of circulating cancer-related biomarkers

Integrated Analysis of Exosomal Protein Biomarkers on Alternating Current Electrokinetic Chips Enables Rapid Detection of Pancreatic Cancer in Patient Blood

(2018) Jean M. Lewis et al.   ACS Nano

Exosome levels in human body fluids: A tumor marker by themselves?

(2017) Francesco Cappello et al.   EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip

(2017) Myoung-Hwan Park et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine

(2017) Jose C. Contreras-Naranjo et al.   LAB ON A CHIP

An integrated microfluidic chip for one-step isolation of circulating tumor cells

(2017) Tae Yoon Lee et al.   SENSORS AND ACTUATORS B-CHEMICAL

The utilization of optically-induced-dielectrophoresis (ODEP)-based virtual cell filters in a microfluidic system for continuous isolation and purification of circulating tumour cells (CTCs) based on their size characteristics

(2017) Wen-Pin Chou et al.   SENSORS AND ACTUATORS B-CHEMICAL

Exosomes as Reconfigurable Therapeutic Systems

(2017) R. Steven Conlan et al.   TRENDS IN MOLECULAR MEDICINE

Exosome-Based Cancer Therapy: Implication for Targeting Cancer Stem Cells

(2017) Jinheng Wang et al.   Frontiers in Pharmacology

Labeling Extracellular Vesicles for Nanoscale Flow Cytometry

(2017) Aizea Morales-Kastresana et al.   Scientific Reports

Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices

(2016) Jina Ko et al.   ANALYST

Cancer: a global concern that demands new detection technologies

(2016) Steven A. Soper et al.   ANALYST

Isolation and enrichment of circulating biomarkers for cancer screening, detection, and diagnostics

(2016) Kyung-A Hyun et al.   ANALYST

FAST: Size-Selective, Clog-Free Isolation of Rare Cancer Cells from Whole Blood at a Liquid–Liquid Interface

(2016) Tae-Hyeong Kim et al.   ANALYTICAL CHEMISTRY

Exosomes and breast cancer: a comprehensive review of novel therapeutic strategies from diagnosis to treatment

(2016) C-Y Wu et al.   CANCER GENE THERAPY

Circulating exosomes and exosomal microRNAs as biomarkers in gastrointestinal cancer

(2016) R Nedaeinia et al.   CANCER GENE THERAPY

Circulating Tumor Cells: A Review of Non-EpCAM-Based Approaches for Cell Enrichment and Isolation

(2016) M. T. Gabriel et al.   CLINICAL CHEMISTRY

Ultrasensitive microfluidic analysis of circulating exosomes using a nanostructured graphene oxide/polydopamine coating

(2016) Peng Zhang et al.   LAB ON A CHIP

A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

(2016) Zheng Zhao et al.   LAB ON A CHIP

Cell-free DNA (cfDNA): Clinical Significance and Utility in Cancer Shaped By Emerging Technologies

(2016) S. Volik et al.   MOLECULAR CANCER RESEARCH

Nanoscale lateral displacement arrays for the separation of exosomes and colloids down to 20 nm

(2016) Benjamin H. Wunsch et al.   Nature Nanotechnology

Isolation of extracellular vesicle from blood plasma using electrophoretic migration through porous membrane

(2016) Siwoo Cho et al.   SENSORS AND ACTUATORS B-CHEMICAL

Continuous Flow Deformability-Based Separation of Circulating Tumor Cells Using Microfluidic Ratchets

(2016) Emily S. Park et al.   Small

Detecting individual extracellular vesicles using a multicolor in situ proximity ligation assay with flow cytometric readout

(2016) Liza Löf et al.   Scientific Reports

Efficient Capture and Isolation of Tumor-Related Circulating Cell-Free DNA from Cancer Patients Using Electroactive Conducting Polymer Nanowire Platforms

(2016) SeungHyun Jeon et al.   Theranostics

Real time and label free profiling of clinically relevant exosomes

(2016) Abu Ali Ibn Sina et al.   Scientific Reports

Acoustic Purification of Extracellular Microvesicles

(2015) Kyungheon Lee et al.   ACS Nano

Exosomes: improved methods to characterize their morphology, RNA content, and surface protein biomarkers

(2015) Yueting Wu et al.   ANALYST

Rapid inertial solution exchange for enrichment and flow cytometric detection of microvesicles

(2015) Jaideep S. Dudani et al.   Biomicrofluidics

Exosomes in cancer: small particle, big player

(2015) Xu Zhang et al.   Journal of Hematology & Oncology

Do Circulating Tumor Cells, Exosomes, and Circulating Tumor Nucleic Acids Have Clinical Utility?

(2015) Bert Gold et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

Size and deformability based separation of circulating tumor cells from castrate resistant prostate cancer patients using resettable cell traps

(2015) Xi Qin et al.   LAB ON A CHIP

A microfluidic device for rapid quantification of cell-free DNA in patients with severe sepsis

(2015) Jun Yang et al.   LAB ON A CHIP

Exosome isolation: a microfluidic road-map

(2015) A. Liga et al.   LAB ON A CHIP

Methods of isolating extracellular vesicles impact down-stream analyses of their cargoes

(2015) Douglas D. Taylor et al.   METHODS

A microfluidic device for label-free, physical capture of circulating tumor cell clusters

(2015) A Fatih Sarioglu et al.   NATURE METHODS

Extracellular Vesicles in Cancer: Exosomes, Microvesicles and the Emerging Role of Large Oncosomes

(2015) Valentina R. Minciacchi et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol

(2015) Mikhail A. Livshits et al.   Scientific Reports

Exosome and Exosomal MicroRNA: Trafficking, Sorting, and Function

(2015) Jian Zhang et al.   GENOMICS PROTEOMICS & BIOINFORMATICS

A review of exosome separation techniques and characterization of B16-F10 mouse melanoma exosomes with AF4-UV-MALS-DLS-TEM

(2014) Kevin E. Petersen et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging

(2014) Ling Zhu et al.   ANALYTICAL CHEMISTRY

Detecting Exosomes Specifically: A Multiplexed Device Based on Alternating Current Electrohydrodynamic Induced Nanoshearing

(2014) Ramanathan Vaidyanathan et al.   ANALYTICAL CHEMISTRY

Determination of Exosome Concentration in Solution Using Surface Plasmon Resonance Spectroscopy

(2014) Déborah L. M. Rupert et al.   ANALYTICAL CHEMISTRY

Microfluidic isolation of cancer-cell-derived microvesicles from hetergeneous extracellular shed vesicle populations

(2014) Steven M. Santana et al.   BIOMEDICAL MICRODEVICES

Circulating Tumor DNA as a Liquid Biopsy for Cancer

(2014) E. Heitzer et al.   CLINICAL CHEMISTRY

Dielectrophoretic isolation and detection of cancer-related circulating cell-free DNA biomarkers from blood and plasma

(2014) Avery Sonnenberg et al.   ELECTROPHORESIS

Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012

(2014) Jacques Ferlay et al.   INTERNATIONAL JOURNAL OF CANCER

Liquid Biopsies: Genotyping Circulating Tumor DNA

(2014) Luis A. Diaz et al.   JOURNAL OF CLINICAL ONCOLOGY

Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing

(2014) E. van der Pol et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

A multi-scale PDMS fabrication strategy to bridge the size mismatch between integrated circuits and microfluidics

(2014) Melaku Muluneh et al.   LAB ON A CHIP

Rare cell isolation and analysis in microfluidics

(2014) Yuchao Chen et al.   LAB ON A CHIP

Integrated immunoisolation and protein analysis of circulating exosomes using microfluidic technology

(2014) Mei He et al.   LAB ON A CHIP

Microfluidic device (ExoChip) for on-chip isolation, quantification and characterization of circulating exosomes

(2014) Shailender Singh Kanwar et al.   LAB ON A CHIP

Inertial microfluidic physics

(2014) Hamed Amini et al.   LAB ON A CHIP

Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor

(2014) Hyungsoon Im et al.   NATURE BIOTECHNOLOGY

Microfluidic, marker-free isolation of circulating tumor cells from blood samples

(2014) Nezihi Murat Karabacak et al.   Nature Protocols

Ex vivo culture of circulating breast tumor cells for individualized testing of drug susceptibility

(2014) M. Yu et al.   SCIENCE

Blood-Based Analyses of Cancer: Circulating Tumor Cells and Circulating Tumor DNA

(2014) D. A. Haber et al.   Cancer Discovery

Antibody-coupled monolithic silica microtips for highthroughput molecular profiling of circulating exosomes

(2014) Koji Ueda et al.   Scientific Reports

Circulating cell-free DNA as a promising biomarker in patients with gastric cancer: diagnostic validity and significant reduction of cfDNA after surgical resection

(2014) Kyongchol Kim et al.   Annals of Surgical Treatment and Research

Clinical Applications of Circulating Tumor Cells in Lung Cancer Patients by CellSearch System

(2014) Anna Truini et al.   Frontiers in Oncology

Magnetic Nanosensor for Detection and Profiling of Erythrocyte-Derived Microvesicles

(2013) Junsung Rho et al.   ACS Nano

Label-Free Cancer Cell Separation from Human Whole Blood Using Inertial Microfluidics at Low Shear Stress

(2013) Myung Gwon Lee et al.   ANALYTICAL CHEMISTRY

Surface Acoustic Wave Microfluidics

(2013) Leslie Y. Yeo et al.   Annual Review of Fluid Mechanics

Exosomes as new vesicular lipid transporters involved in cell–cell communication and various pathophysiologies

(2013) Michel Record et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Label-free isolation of circulating tumor cells in microfluidic devices: Current research and perspectives

(2013) Igor Cima et al.   Biomicrofluidics

Detection and isolation of circulating tumor cells: Principles and methods

(2013) Hadi Esmaeilsabzali et al.   BIOTECHNOLOGY ADVANCES

Exosomes in cancer development, metastasis, and drug resistance: a comprehensive review

(2013) Asfar S. Azmi et al.   CANCER AND METASTASIS REVIEWS

Rapid Electrokinetic Isolation of Cancer-Related Circulating Cell-Free DNA Directly from Blood

(2013) A. Sonnenberg et al.   CLINICAL CHEMISTRY

Dielectrophoretic isolation and detection of cfc-DNA nanoparticulate biomarkers and virus from blood

(2013) Avery Sonnenberg et al.   ELECTROPHORESIS

Exosomes as Intercellular Signaling Organelles Involved in Health and Disease: Basic Science and Clinical Applications

(2013) Chiara Corrado et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Exosomes in tumor microenvironment influence cancer progression and metastasis

(2013) Christoph Kahlert et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles

(2013) Zongxing Wang et al.   LAB ON A CHIP

Advances and critical concerns with the microfluidic enrichments of circulating tumor cells

(2013) Kyung-A Hyun et al.   LAB ON A CHIP

Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells

(2013) Majid Ebrahimi Warkiani et al.   LAB ON A CHIP

Size-selective collection of circulating tumor cells using Vortex technology

(2013) Elodie Sollier et al.   LAB ON A CHIP

Liquid biopsy: monitoring cancer-genetics in the blood

(2013) Emily Crowley et al.   Nature Reviews Clinical Oncology

Influence of Plasma Processing on Recovery and Analysis of Circulating Nucleic Acids

(2013) Karen Page et al.   PLoS One

Circulating Breast Tumor Cells Exhibit Dynamic Changes in Epithelial and Mesenchymal Composition

(2013) M. Yu et al.   SCIENCE

Inertial Focusing for Tumor Antigen-Dependent and -Independent Sorting of Rare Circulating Tumor Cells

(2013) E. Ozkumur et al.   Science Translational Medicine

The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo

(2013) Jeoffrey Schageman et al.   Biomed Research International

Aptamer-Enabled Efficient Isolation of Cancer Cells from Whole Blood Using a Microfluidic Device

(2012) Weian Sheng et al.   ANALYTICAL CHEMISTRY

Exosomes: Current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials

(2012) Alexander V. Vlassov et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Circulating tumour cells escape from EpCAM-based detection due to epithelial-to-mesenchymal transition

(2012) Tobias M Gorges et al.   BMC CANCER

Nanoparticle tracking analysis monitors microvesicle and exosome secretion from immune cells

(2012) Chin Y. Soo et al.   IMMUNOLOGY

Single vs. swarm detection of microparticles and exosomes by flow cytometry

(2012) E. Van Der POL et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Microfluidic filtration system to isolate extracellular vesicles from blood

(2012) Ryan T. Davies et al.   LAB ON A CHIP

A combined micromagnetic-microfluidic device for rapid capture and culture of rare circulating tumor cells

(2012) Joo H. Kang et al.   LAB ON A CHIP

Fluorescent labeling of nano-sized vesicles released by cells and subsequent quantitative and qualitative analysis by high-resolution flow cytometry

(2012) Els J van der Vlist et al.   Nature Protocols

Ultrasensitive Clinical Enumeration of Rare Cells ex Vivo Using a Micro-Hall Detector

(2012) D. Issadore et al.   Science Translational Medicine

Aptamers and Their Biological Applications

(2012) Kyung-Mi Song et al.   SENSORS

Rare Cell Separation and Analysis by Magnetic Sorting

(2011) Maciej Zborowski et al.   ANALYTICAL CHEMISTRY

Genomic analysis of circulating cell-free DNA infers breast cancer dormancy

(2011) J. A. Shaw et al.   GENOME RESEARCH

Body fluid derived exosomes as a novel template for clinical diagnostics

(2011) Sascha Keller et al.   Journal of Translational Medicine

Miniature magnetic resonance system for point-of-care diagnostics

(2011) David Issadore et al.   LAB ON A CHIP

Circulating tumor cells: the Grand Challenge

(2011) Jaap den Toonder   LAB ON A CHIP

Forthcoming Lab on a Chip tutorial series on acoustofluidics: Acoustofluidics—exploiting ultrasonic standing wave forces and acoustic streaming in microfluidic systems for cell and particle manipulation

(2011) Henrik Bruus et al.   LAB ON A CHIP

Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis

(2011) Rebecca A. Dragovic et al.   Nanomedicine-Nanotechnology Biology and Medicine

3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood

(2010) Siyang Zheng et al.   BIOMEDICAL MICRODEVICES

Heterogeneous detection of circulating tumor cells in patients with colorectal cancer by immunomagnetic enrichment using different EpCAM-specific antibodies

(2010) Dalibor Antolovic et al.   BMC BIOTECHNOLOGY

Side-by-side analysis of five clinically tested anti-EpCAM monoclonal antibodies

(2010) Markus Münz et al.   Cancer Cell International

Utilizing circulating tumor cells: challenges and pitfalls

(2010) Gerhardt Attard et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

Standardization of platelet-derived microparticle enumeration by flow cytometry with calibrated beads: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop

(2010) R. LACROIX et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Exosomes/microvesicles as a mechanism of cell-to-cell communication

(2010) Giovanni Camussi et al.   KIDNEY INTERNATIONAL

Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)

(2010) Hadi Shafiee et al.   LAB ON A CHIP

Single-cell microfluidic impedance cytometry: a review

(2010) Tao Sun et al.   Microfluidics and Nanofluidics

Isolation of circulating tumor cells using a microvortex-generating herringbone-chip

(2010) S. L. Stott et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enrichment of Cancer Cells Using Aptamers Immobilized on a Microfluidic Channel

(2009) Joseph A. Phillips et al.   ANALYTICAL CHEMISTRY

Microdevice for the isolation and enumeration of cancer cells from blood

(2009) Swee Jin Tan et al.   BIOMEDICAL MICRODEVICES

Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT–PCR in patients with metastatic breast cancer

(2009) I Van der Auwera et al.   BRITISH JOURNAL OF CANCER

Exosomal MicroRNA: A Diagnostic Marker for Lung Cancer

(2009) Guilherme Rabinowits et al.   Clinical Lung Cancer

Exosomes – vesicular carriers for intercellular communication

(2009) Mikael Simons et al.   CURRENT OPINION IN CELL BIOLOGY

Isolation of rare cells from cell mixtures by dielectrophoresis

(2009) Peter R. C. Gascoyne et al.   ELECTROPHORESIS

Isolation of tumor cells using size and deformation

(2009) Hisham Mohamed et al.   JOURNAL OF CHROMATOGRAPHY A

Inertial microfluidics for continuous particle separation in spiral microchannels

(2009) Sathyakumar S. Kuntaegowdanahalli et al.   LAB ON A CHIP

Microfluidic isolation and transcriptome analysis of serum microvesicles

(2009) Chihchen Chen et al.   LAB ON A CHIP

Microfluidics Digital PCR Reveals a Higher than Expected Fraction of Fetal DNA in Maternal Plasma

(2008) F. M. F. Lun et al.   CLINICAL CHEMISTRY

CD44 and EpCAM: Cancer-Initiating Cell Markers

(2008) Rachid Marhaba et al.   CURRENT MOLECULAR MEDICINE

MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer

(2008) Douglas D. Taylor et al.   GYNECOLOGIC ONCOLOGY

Discrimination between exosomes and HIV-1: Purification of both vesicles from cell-free supernatants

(2008) Réjean Cantin et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Proteomic Analysis of Exosomes from Human Neural Stem Cells by Flow Field-Flow Fractionation and Nanoflow Liquid Chromatography−Tandem Mass Spectrometry

(2008) Dukjin Kang et al.   JOURNAL OF PROTEOME RESEARCH

Highly Efficient Circulating Tumor Cell Isolation from Whole Blood and Label-Free Enumeration Using Polymer-Based Microfluidics with an Integrated Conductivity Sensor

(2008) André A. Adams et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers

(2008) Johan Skog et al.   NATURE CELL BIOLOGY

Detection of Mutations inEGFRin Circulating Lung-Cancer Cells

(2008) Shyamala Maheswaran et al.   NEW ENGLAND JOURNAL OF MEDICINE