Journal
LAB ON A CHIP
Volume 15, Issue 19, Pages 3925-3933Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5lc00681c
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Funding
- Ontario Ministry for Research and Innovation through Ontario Research Fund - Research Excellence Program
- Canada Research Chairs Program
- Natural Sciences and Engineering Research Council of Canada (NSERC)
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A rapid and accurate method to identify severe sepsis patients at high risk of death is critically needed for clinical practice. In a recent study, the concentration of cell-free DNA (cfDNA) in blood was found to be a prognostic indicator for ICU mortality in patients with severe sepsis. However, current DNA quantification techniques are time-consuming and involve extensive sample preparation. In this paper, we demonstrate a low-cost microfluidic device capable of rapid quantification of cfDNA in a small droplet (<10 mu l) of blood plasma and whole blood in 5 min using only electrical power. The cfDNA in samples is selectively labeled by PicoGreen and is extracted and concentrated by electrophoresis into a gel by application of a DC potential of 9 V. This device has potential as a prognostic tool for early and rapid assessment of septic patients.
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