Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

CXCR4 signaling is controlled by immobilization at the plasma membrane

(2016) Elena Beletkaia et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Targeted Elimination of G Proteins and Arrestins Defines Their Specific Contributions to Both Intensity and Duration of G Protein-coupled Receptor Signaling

(2016) Elisa Alvarez-Curto et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The β-Arrestins: Multifunctional Regulators of G Protein-coupled Receptors

(2016) Jeffrey S. Smith et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mutations of Vasopressin Receptor 2 Including Novel L312S Have Differential Effects on Trafficking

(2016) Anatoly Tiulpakov et al.   MOLECULAR ENDOCRINOLOGY

The conformational signature of β-arrestin2 predicts its trafficking and signalling functions

(2016) Mi-Hye Lee et al.   NATURE

β-Arrestin biosensors reveal a rapid, receptor-dependent activation/deactivation cycle

(2016) Susanne Nuber et al.   NATURE

Versatile protein tagging in cells with split fluorescent protein

(2016) Daichi Kamiyama et al.   Nature Communications

Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET

(2016) Yoon Namkung et al.   Nature Communications

A simple method to generate stable cell lines for the analysis of transient protein-protein interactions

(2016) Emilia Elizabeth Savage et al.   BIOTECHNIQUES

Fast and high resolution single-cell BRET imaging

(2016) Elise Goyet et al.   Scientific Reports

NanoBRET—A Novel BRET Platform for the Analysis of Protein–Protein Interactions

(2015) Thomas Machleidt et al.   ACS Chemical Biology

Self-Assembling NanoLuc Luciferase Fragments as Probes for Protein Aggregation in Living Cells

(2015) Jia Zhao et al.   ACS Chemical Biology

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Application of BRET to monitor ligand binding to GPCRs

(2015) Leigh A Stoddart et al.   NATURE METHODS

Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

(2015) Kathrin Schumann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Target engagement and drug residence time can be observed in living cells with BRET

(2015) Matthew B. Robers et al.   Nature Communications

A generic strategy for CRISPR-Cas9-mediated gene tagging

(2015) Daniel H. Lackner et al.   Nature Communications

CXCR4 over-expression and survival in cancer: A system review and meta-analysis

(2015) Hongli Zhao et al.   Oncotarget

Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9

(2015) Alejandro Rojas-Fernandez et al.   Scientific Reports

Genome editing of CXCR4 by CRISPR/cas9 confers cells resistant to HIV-1 infection

(2015) Panpan Hou et al.   Scientific Reports

CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells

(2015) Michael Ratz et al.   Scientific Reports

Efficient CRISPR-Cas9-Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus

(2015) Florian T. Merkle et al.   Cell Reports

A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging

(2014) Marvin E. Tanenbaum et al.   CELL

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

(2014) Pankaj K. Mandal et al.   Cell Stem Cell

Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells

(2014) Robert Mahen et al.   MOLECULAR BIOLOGY OF THE CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

DNA repair mechanisms in dividing and non-dividing cells

(2013) Teruaki Iyama et al.   DNA REPAIR

The Bile Acid Receptor TGR5 Does Not Interact with β-Arrestins or Traffic to Endosomes but Transmits Sustained Signals from Plasma Membrane Rafts

(2013) Dane D. Jensen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Conformational biosensors reveal GPCR signalling from endosomes

(2013) Roshanak Irannejad et al.   NATURE

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

The transience of transient overexpression

(2013) Toby J Gibson et al.   NATURE METHODS

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate

(2012) Mary P. Hall et al.   ACS Chemical Biology

Effect of enhanced Renilla luciferase and fluorescent protein variants on the Förster distance of Bioluminescence resonance energy transfer (BRET)

(2012) Helen Dacres et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Sensitive and High Resolution Localization and Tracking of Membrane Proteins in Live Cells with BRET

(2012) Tien-Hung Lan et al.   TRAFFIC

Heteromerization of angiotensin receptors changes trafficking and arrestin recruitment profiles

(2011) Enzo R. Porrello et al.   CELLULAR SIGNALLING

CXCR7/CXCR4 Heterodimer Constitutively Recruits β-Arrestin to Enhance Cell Migration

(2011) Fabien M. Décaillot et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Efficient mutagenesis of the rhodopsin gene in rod photoreceptor neurons in mice

(2011) Fung Chan et al.   NUCLEIC ACIDS RESEARCH

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

Internalization Dissociates β2-Adrenergic Receptors

(2011) Tien-Hung Lan et al.   PLoS One

Application of G Protein-Coupled Receptor-Heteromer Identification Technology to Monitor β-Arrestin Recruitment to G Protein-Coupled Receptor Heteromers

(2010) Heng B. See et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Multiplexing of Multicolor Bioluminescence Resonance Energy Transfer

(2010) Billy Breton et al.   BIOPHYSICAL JOURNAL

Protein-protein interactions monitored in cells from transgenic mice using bioluminescence resonance energy transfer

(2010) Martin Audet et al.   FASEB JOURNAL

Site-specific Phosphorylation of CXCR4 Is Dynamically Regulated by Multiple Kinases and Results in Differential Modulation of CXCR4 Signaling

(2010) John M. Busillo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

β2-Adrenergic Receptor Signaling in the Cardiac Myocyte is Modulated by Interactions With CXCR4

(2010) Thomas J LaRocca et al.   JOURNAL OF CARDIOVASCULAR PHARMACOLOGY

Rab GTPases Bind at a Common Site within the Angiotensin II Type I Receptor Carboxyl-Terminal Tail: Evidence that Rab4 Regulates Receptor Phosphorylation, Desensitization, and Resensitization

(2010) J. L. Esseltine et al.   MOLECULAR PHARMACOLOGY

CXCR7 heterodimerizes with CXCR4 and regulates CXCL12-mediated G protein signaling

(2009) A. Levoye et al.   BLOOD

Agonist-Independent Interactions between β-Arrestins and Mutant Vasopressin Type II Receptors Associated with Nephrogenic Syndrome of Inappropriate Antidiuresis

(2009) Martina Kocan et al.   MOLECULAR ENDOCRINOLOGY

Functional complementation of high-efficiency resonance energy transfer: a new tool for the study of protein binding interactions in living cells

(2007) Paola Molinari et al.   BIOCHEMICAL JOURNAL