Article
Cell Biology
Matteo Vecellio, Elvezia Maria Paraboschi, Angela Ceribelli, Natasa Isailovic, Francesca Motta, Giulia Cardamone, Michela Robusto, Rosanna Asselta, Sonia Brescianini, Francesco Sacrini, Antonio Costanzo, Maria De Santis, Maria Antonietta Stazi, Stefano Duga, Carlo Selmi
Summary: A study on monozygotic twins revealed DNA methylation differences in psoriatic disease patients compared to controls, involving genes related to the TGF-beta signaling pathway. Transcriptome data further identified the IL-6/JAK/STAT3 and TNF-alpha pathways as key players in the disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Dermatology
Ashild O. Solvin, Konika Chawla, Lene C. Olsen, Siv Anita Hegre, Kjersti Danielsen, Marita Jenssen, Anne-Sofie Furberg, Marit Saunes, Kristian Hveem, Pal Saetrom, Mari Loset
Summary: This study provides a comprehensive overview of the miRNAome in psoriatic skin, identifying a miRNA signature related to psoriasis severity and uncovering functionally enriched terms related to thyroid hormone signaling, insulin resistance, and infectious diseases. The results may represent molecular links between psoriasis and related comorbidities, highlighting potential directions for future functional studies in biomarker identification and treatment targets.
EXPERIMENTAL DERMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yong Wang, Yong Yan, Huiwen Yu, Guangrong Jiang, Shiying Zhao, Meng Yi
Summary: This study aimed to investigate the expression and function of VEGFRs in normal epidermis and keratinocytes of psoriatic lesions. The results showed that compared to healthy individuals, keratinocytes in psoriatic lesion regions significantly enhanced their expression of VEGFR-1,2,3. The overexpression of VEGFR-1,2,3 in psoriatic lesions may be encouraged by VEGF and Ca ions.
CELLULAR AND MOLECULAR BIOLOGY
(2023)
Article
Veterinary Sciences
Rosanna Marsella, Rachel Wilkes, Kim Ahrens
Summary: This study compares the suitability of canine progenitor epidermal keratinocytes (CPEK) and normal keratinocytes (NK) in skin barrier studies. The results show that CPEK grown in monolayer exhibit differences in cell size, granularity, and tight junction expression compared to NK, indicating that they are not suitable for permeability studies.
VETERINARY SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Mohammad Farhadian, Seyed Aabbas Rafat, Bahman Panahi, Esmaeil Ebrahimie
Summary: The gene expression and regulation during lactation in Ghezel sheep breed were studied, revealing 75 differentially expressed genes between the before and after peak stages of lactation. DEGs were mainly involved in metabolic processes and oxidative phosphorylation. PPI network analysis highlighted the contributions of PPAR signaling and other pathways in the lactation process.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Immunology
Jared Liu, Sugandh Kumar, Julie Hong, Zhi-Ming Huang, Diana Paez, Maria Castillo, Maria Calvo, Hsin-Wen Chang, Daniel D. D. Cummins, Mimi Chung, Samuel Yeroushalmi, Erin Bartholomew, Marwa Hakimi, Chun Jimmie Ye, Tina Bhutani, Mehrdad Matloubian, Lianne S. S. Gensler, Wilson Liao
Summary: Early diagnosis of psoriatic arthritis (PSA) is important but challenging. In this study, single cell profiling was used to compare the immunocyte populations of individuals with PSA, individuals with cutaneous psoriasis (PSO) alone, and healthy individuals. Machine learning classification based on cell type-specific gene and protein expression differences achieved high accuracy in distinguishing PSA from PSO and healthy groups. These findings demonstrate the utility of machine learning-based diagnostics for PSA.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tiancheng Wang, Zhenwei Zhou, Enjing Luo, Jinghong Zhong, Daqing Zhao, Haisi Dong, Baojin Yao
Summary: This study compared the gene expression patterns between keratinocytes and fibroblasts, identifying a large number of differentially expressed genes. Keratinocytes and fibroblasts have distinct molecular characteristics, providing a resource for investigating novel biomarkers related to skin diseases.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Zhishen Jiang, Zijian Zhang, Xiaotong You, Li Ye, Weimin Lin, Liu Liu, Yubin Cao, Jian Pan
Summary: This study examined the crosstalk network between T cells, epithelial cells, and fibroblasts in the tumor microenvironment of oral squamous cell carcinoma (OSCC) and investigated their prognostic values. Key prognostic features were identified using Cox analysis by integrating bulk transcriptome data and clinical parameters. Functional analysis and immune infiltration were performed to explore the underlying mechanisms.
Article
Genetics & Heredity
Kai Chen, Qi Wang, Xinxin Liu, Feng Wang, Yongsu Ma, Shupeng Zhang, Zhijiang Shao, Yinmo Yang, Xiaodong Tian
Summary: This study utilized single-cell RNA sequencing and database information to identify reliable markers for malignant ductal cells in pancreatic ductal adenocarcinoma (PDAC) and developed a potential prognostic model based on immune-related signatures.
Article
Immunology
Sicheng Wan, Ulrich Aymard Ekomi Moure, Ruochen Liu, Chaolong Liu, Kun Wang, Longfei Deng, Ping Liang, Hongjuan Cui
Summary: This study investigates the role of necroptosis in glioma, constructs a necroptosis-related signature (NRS) for predicting prognosis, and explores the correlation between NRS and tumor immune environment, chemotherapy sensitivity, etc. Experimental and data analysis reveal the importance of necroptosis-related genes in glioma.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Hang Zheng, Heshu Liu, Yang Ge, Xin Wang
Summary: The study established a nine-gene prognostic CAF-related signature for CRC, which was validated in multiple cohorts. Patients with higher CAF risk scores showed adverse prognosis and higher CAF infiltrations. The potential strategy of combining chemotherapy with anti-CAF therapy was suggested to improve the efficacy of T-cell based immunotherapies.
CANCER CELL INTERNATIONAL
(2021)
Article
Oncology
Ke Wang, Mingyang Yu, Zhouzhou Zhang, Rong Yin, Qifeng Chen, Xuezhi Zhao, Hongqi Yu
Summary: A novel natural killer cell marker gene signature (NKMS) was constructed to predict prognosis and therapeutic response of clear cell renal cell carcinoma (ccRCC) patients. This signature showed exceptional predictive capability in determining prognosis and treatment response in ccRCC patients, and can serve as an independent predictive biomarker for individualized treatment selection.
TRANSLATIONAL CANCER RESEARCH
(2023)
Article
Oncology
Ke Wang, Mingyang Yu, Zhouzhou Zhang, Rong Yin, Qifeng Chen, Xuezhi Zhao, Hongqi Yu
Summary: This study constructed a NK cell marker gene signature to predict prognosis and therapeutic response of ccRCC patients. The signature showed exceptional predictive capability and was able to identify high-risk patients. High-risk patients exhibited higher tumor mutation burden and immune cell infiltration, associated with expression of genes negatively regulating anti-tumor immunity.
TRANSLATIONAL CANCER RESEARCH
(2023)
Article
Medicine, Research & Experimental
Allison C. Billi, Jessica E. Ludwig, Yi Fritz, Richard Rozic, William R. Swindell, Lam C. Tsoi, Dennis Gruzska, Shahla Abdollahi-Roodsaz, Xianying Xing, Doina Diaconu, Ranjitha Uppala, Maya Camhi, Philip A. Klenotic, Mrinal K. Sarkar, M. Elaine Husni, Jose U. Scher, Christine McDonald, J. Michelle Kahlenberg, Ronald J. Midura, Johann E. Gudjonsson, Nicole L. Ward
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Cell Biology
Cory L. Simpson, Mariko K. Tokito, Ranjitha Uppala, Mrinal K. Sarkar, Johann E. Gudjonsson, Erika L. F. Holzbaur
Summary: This study reveals that regulated breakdown of mitochondria is critical for epidermal development, with keratinocytes in the upper layers initiating mitochondrial fragmentation, depolarization, and acidification. The mitophagy receptor NIX and DRP1 play essential roles in driving epidermal morphogenesis by promoting mitochondrial degradation in the terminal differentiation of keratinocytes.
Article
Dermatology
Shuai Shao, Lam C. Tsoi, William R. Swindell, Jiaoling Chen, Ranjitha Uppala, Allison C. Billi, Xianying Xing, Chang Zeng, Mrinal K. Sarkar, Rachael Wasikowski, Yanyun Jiang, Joseph Kirma, Jingru Sun, Olesya Plazyo, Gang Wang, Paul W. Harms, John J. Voorhees, Nicole L. Ward, Feiyang Ma, Matteo Pellegrini, Alexander Merleev, Bethany E. Perez White, Robert L. Modlin, Bogi Andersen, Emanual Maverakis, Stephan Weidinger, J. Michelle Kahlenberg, Johann E. Gudjonsson
Summary: The research reveals the critical role of IRAK2 in the severity of atopic dermatitis and psoriasis, with inhibition of IRAK2 normalizing differentiation and inflammation. These findings suggest that IRAK2 promotes feed-forward amplification of inflammatory responses in the skin through modulation of differentiation pathways and inflammatory responses.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
William R. Swindell, Manpreet Randhawa, Geovani Quijas, Krzysztof Bojanowski, Ratan K. Chaudhuri
Summary: The study showed that THDC is a poor antioxidant that degrades rapidly when exposed to singlet oxygen, but this degradation can be prevented by combining with acetyl zingerone (AZ). The combination of THDC and AZ increased expression of genes associated with phospholipid homeostasis and keratinocyte differentiation, while reducing pro-inflammatory effects and improving collagen production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Allergy
Lam C. Tsoi, Matthew T. Patrick, Shao Shuai, Mrinal K. Sarkar, Sunyi Chi, Bethany Ruffino, Allison C. Billi, Xianying Xing, Ranjitha Uppala, Cheng Zang, Joseph Fullmer, Zhi He, Emanual Maverakis, Nehal N. Mehta, Bethany E. Perez White, Spiro Getsios, Yolanda Helfrich, John J. Voorhees, J. Michelle Kahlenberg, Stephan Weidinger, Johann E. Gudjonsson
Summary: This study evaluates the use of baseline molecular expression profiling to assess treatment outcome for patients with psoriasis. The results show a correlation between clinical response and molecular changes, particularly for genes responding to IL-17A/TNF in keratinocytes. Baseline gene expressions in nonlesional skin were found to be the best marker of treatment response, and cytokine gene signatures enriched in baseline nonlesional skin had strong correlations with treatment improvement.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Review
Dermatology
Di Yan, Johann E. Gudjonsson, Stephanie Le, Emanual Maverakis, Olesya Plazyo, Christopher Ritchlin, Jose U. Scher, Roopesh Singh, Nicole L. Ward, Stacie Bell, Wilson Liao
Summary: Psoriasis is a chronic inflammatory condition characterized by systemic immune dysregulation. Recent advances in genetics, microbiology, immunology, and mouse models have shed light on the complex interplay between heritable and microenvironmental factors in driving psoriatic inflammation. These scientific advancements have paved the way for a more personalized approach to treating psoriatic disease.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Medicine, Research & Experimental
Shuai Shao, Jiaoling Chen, William R. Swindell, Lam C. Tsoi, Xianying Xing, Feiyang Ma, Ranjitha Uppala, Mrinal K. Sarkar, Olesya Plazyo, Allison C. Billi, Rachael Wasikowski, Kathleen M. Smith, Prisca Honore, Victoria E. Scott, Emanual Maverakis, J. Michelle Kahlenberg, Gang Wang, Nicole L. Ward, Paul W. Harms, Johann E. Gudjonsson
Summary: Through global transcriptomic profiling, it was found that there is a high overlap between plaque psoriasis and pityriasis rubra pilaris skin lesions, indicating a common molecular signature between these skin disorders. The study highlights the important role of phospholipases as regulators of epidermal barrier homeostasis and inflammation, identifying them as a shared pathogenic mechanism between PRP and psoriasis, and as potential therapeutic targets for both diseases.
Article
Dermatology
Lam C. Tsoi, Xianying Xing, Enze Xing, Rachael Wasikowski, Shuai Shao, Chang Zeng, Olesya Plazyo, Joseph Kirma, Yanyung Jiang, Allison C. Billi, Mrinal K. Sarkar, Jessica L. Turnier, Ranjitha Uppala, Kathleen M. Smith, Yolanda Helfrich, John J. Voorhees, Emanual Maverakis, Robert L. Modlin, J. Michelle Kahlenberg, Victoria E. Scott, Johann E. Gudjonsson
Summary: This study demonstrates that tape stripping with optimized high-resolution transcriptomic profiling can effectively assess and characterize inflammatory responses in the skin. By comparing different samples and sampling methods, the study shows that tape stripping can capture the transcriptome of the upper layers of the skin, providing insights into the molecular components of the immune amplification pathway in psoriasis. Additionally, the study suggests that nonlesional psoriatic skin exhibits activated, proinflammatory changes, which are not captured by full-thickness skin biopsy transcriptome profiling.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Review
Dermatology
Roopesh Singh Gangwar, Johann E. Gudjonsson, Nicole L. Ward
Summary: This comprehensive review compares and discusses genetic mouse models, xenograft approaches, and cytokine injections and topical imiquimod application in mice for studying psoriasis. It provides a detailed description and outcomes of these models and methods, serving as a valuable resource for researchers interested in working with psoriasis animal models.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Review
Genetics & Heredity
Alanna Shefler, Matthew T. Patrick, Rachael Wasikowski, Jiahan Chen, Mrinal K. Sarkar, Johann E. Gudjonsson, Lam C. Tsoi
Summary: This review characterizes prominent skin-expressing lncRNAs using large scale genomic data, providing insights into their potential roles in the pathology of inflammatory skin diseases like atopic dermatitis and psoriasis. The review also highlights the cell type specificity of lncRNAs and their potential as mediators in cytokine-stimulated pathways.
FRONTIERS IN GENETICS
(2022)
Article
Chemistry, Medicinal
William R. Swindell, Krzysztof Bojanowski, Ratan K. Chaudhuri
Summary: Dimethyl fumarate (DMF) is commonly used for treating RRMS but has adverse effects. To improve tolerability, novel derivatives have been developed. In this study, the effects of fumarates on gene expression in astrocytes were compared, and it was found that IDMF had the greatest impact on gene expression, inhibiting NF-kappa B and IRF1 expression, thus reducing inflammation and pyroptosis.
Article
Cell Biology
Arnaud Goepfert, Carmen Barske, Sylvie Lehmann, Emmanuelle Wirth, Joschka Willemsen, Johann E. Gudjonsson, Nicole L. Ward, Mrinal K. Sarkar, Rene Hemmig, Frank Kolbinger, Jean-Michel Rondeau
Summary: Through crystallography, biophysics, and mutational studies, this research demonstrates that IL-17A, IL-17F, and IL-17A/F induce IL-17RA dimerization, leading to the formation of a 2:2:2 hexameric signaling assembly. The formation of the IL-17 signalosome potentiates IL-17-induced IL-36 gamma and CXCL1 mRNA expression in human keratinocytes compared to a dimerization-defective IL-17RA variant.
Article
Multidisciplinary Sciences
Jeffrey Y. Zhou, Mrinal K. Sarkar, Ken Okamura, John E. Harris, Johann E. Gudjonsson, Katherine A. Fitzgerald
Summary: The accumulation of cytosolic DNA leads to the production of type I IFNs, maturation of IL-1 cytokines, and pyroptotic cell death. Caspase-1 cleaves pro-IL-1 beta to generate mature cytokines and gasdermin D, which facilitate the release of IL-1 and pyroptotic cell death. AIM2 serves as a sensor for dsDNA, leading to caspase-1 activation, while in human monocytes, cGAS-STING acting upstream of NLRP3 mediates the inflammasome response to dsDNA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)