Article
Chemistry, Analytical
Liyan Zhang, Xiaoting Liu, Nan Zhang, Xianqiang Liu, Wei Jiang
Summary: This study developed an enzyme-free and sensitive detection method for FEN1 using a magnetic separation-assisted cascade HCR strategy. The strategy achieved a low detection limit for FEN1 and has the potential to be important in early tumor diagnosis and prognosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Jingjing Zhang, Chunyuan Song, Yunfeng Zhu, Hongyu Gan, Xinyue Fang, Qian Peng, Jingrong Xiong, Chen Dong, Caiqin Han, Lianhui Wang
Summary: In this study, a novel one-pot cascade signal amplification strategy integrating the CRISPR/Cas13a system and branched hybridization chain reaction (bHCR) was proposed for highly sensitive and specific SERS assay of disease-related nucleic acids. The method showed low detection limit, good linear calibration curve, and demonstrated good practicality in clinical samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Feng Hong, Chenxi Huang, Long Wu, Miao Wang, Yiping Chen, Yongxin She
Summary: The study proposed a magnetic relaxation sensing method based on gold nanoparticles (Au NPs) for highly sensitive detection of aflatoxin B1 (AFB1). By utilizing a triple self-assembly cascade signal amplification strategy, the sensor showed high specificity and sensitivity, successfully detecting AFB1 in animal feed samples with consistent results.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Mengyan Li, Nian Luo, Xiaofei Liao, Li Zou
Summary: We developed a dual signal amplification strategy for sensitive detection of circulating tumor DNA (ctDNA) based on hybridization chain reaction (HCR) and proximity hybridization-regulated CRISPR/Cas12a. This strategy exhibits high sensitivity toward ctDNA with a low detection limit of 5.43 fM, and it has been successfully applied for ctDNA detection in serum samples, showing great potential in the clinical diagnosis of cancer.
Article
Chemistry, Analytical
Wanling Cui, Guodong Hu, Feng Song, Rui Wang, Zanxia Cao, Junye Zhang, Tiejun Wang, Fanlu Meng, Congcong Shen, Shicai Xu, Jihua Wang
Summary: DNA methyltransferase (MTase) is considered a promising biomarker and therapeutic target, playing a crucial regulatory role in various biological processes. A cascade strand displacement amplification strategy was developed for highly sensitive and label-free fluorescent detection of DNA MTase activity, showing improved detection sensitivity. The strategy also effectively evaluated the inhibitory effects of 5-fluorouracil, benzylpenicillin, and gentamycin on DNA MTase activity, suggesting great potential for new method exploration and early disease diagnosis.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Applied
Ge Wu, Zhengwei Xiong, Suk-Heung Oh, Yanrong Ren, Qiang Wang, Lizhu Yang
Summary: A two-color fluorescent DNA tweezers developed for ultrasensitive detection of OTA shows a good linear range and detection limit, effectively reducing false positive rate in practical food samples.
Article
Chemistry, Analytical
Hong Zhou, Kexin Ding, Binxiao Li, Haiyan Wang, Ningbo Zhang, Jing Liu
Summary: This study developed a nonenzymatic and ultrasensitive method for analyzing human prostate specific antigen, achieving detection of PSA concentration as low as 0.73 μg mL(-1) using a nucleic acid circuit amplification strategy. The sensing method avoids the use of sophisticated equipment and artificial enzymes, reducing experimental complexity and constraints.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biotechnology & Applied Microbiology
Xiaowei Cao, Shengjie Ge, Weiwei Hua, Xinyu Zhou, Wenbo Lu, Yingyan Gu, Zhiyue Li, Yayun Qian
Summary: In this work, a pump-free, high-throughput microfluidic chip coupled with catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) was developed for the detection of gastric cancer (GC) related circulating tumor DNA (ctDNA). The chip exhibited excellent performance in terms of detection sensitivity and speed, with an ultralow limit of detection achieved for the target ctDNAs. The chip also demonstrated good accuracy through comparison with the gold-standard qRT-PCR assay. This rapid, sensitive, and cost-effective SERS microfluidic chip has the potential to be a powerful tool for ctDNA monitoring.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Li Zou, Manjun Zhang, Mengyan Li, Zhiyou Xiao, Liansheng Ling
Summary: This study developed an enzyme-free fluorescent aptasensor using a dual signal amplification strategy for sensitive detection of aflatoxin B1. The aptasensor showed high sensitivity and selectivity, making it suitable for food safety control.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Analytical
Min-Xi Li, Yao Chen, Zeng-Ping Chen, Ru-Qin Yu
Summary: A label-free and sensitive miRNA detection method based on locked nucleic acid assisted repeated fishing amplification strategy was developed, providing a practical alternative for designing cost-effective and label-free miRNA detection methods. The proposed platform achieved a limit of detection value of 2.9 fM for miRNA-122 and obtained quantitative results comparable to qRT-PCR.
Article
Chemistry, Analytical
Hong Hai, Ciping Chen, Dongli Chen, Peijun Li, Yang Shan, Jianping Li
Summary: An ECL DNA biosensor based on Exolli exonuclease assistance and HCR amplification technology was constructed for high-sensitive detection of transgenic rice BT63 DNA. The sensor utilized ExoIII exonuclease and triple-helix DNA molecular switch, combined with HCR and AuNPs@DNA to enable multiple signal amplification. The detection limit was 3.6 x 10(-17) mol/L with good linear relationship in the concentration range 1.0 x 10(-16) to 1.0 x 10(-8) mol/L of the target DNA.
Article
Chemistry, Analytical
Feng Qiu, Xiaoyan Gan, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: This study presents a novel method for sensitive detection of thrombin in human serums. By utilizing target-promoted magnetic nanoparticle-decorated DNA polymers for proximity binding hybridization chain reaction, the detection of thrombin is significantly enhanced.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Duoduo Zhang, Kan Wang, Wei Wei, Yong Liu, Songqin Liu
Summary: The study constructed a multifunctional plasmonic core-satellites nanoprobe for clinical cancer diagnosis and therapy, which can be used for miRNA detection, targeting drug release, and therapy evaluation.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Shihua Luo, Ye Zhang, Guoni Huang, Bo Situ, Xinyi Ye, Maliang Tao, Yifang Huang, Bo Li, Xiujuan Jiang, Qian Wang, Lei Zheng
Summary: This study developed a novel electrochemical biosensor for sensitive detection of ctDNA, utilizing wheel-like catalytic hairpin assembly and frame hybridization chain reaction, which significantly enhances the sensitivity of ctDNA detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Yan-Li Zhu, Ji-Kai Wang, Zeng-Ping Chen, Yu-Jie Zhao, Ru-Qin Yu
Summary: A versatile triple cascade amplification strategy was developed for ultrasensitive simultaneous detection of multiple cancer biomarkers using single particle inductively coupled plasma mass spectrometry (spICP-MS). The spICP-MS platform achieved satisfactory quantitative results for both carcinoembryonic antigen (CEA) and a-fetoprotein (AFP) in human serum samples with accuracy comparable to that of the commercial ELISA kits. Compared with conventional biomarkers detection methods, the proposed spICP-MS platform has the advantages of operational simplicity, ultra-high sensitivity, wide dynamic range, and low background.
Article
Nanoscience & Nanotechnology
Cui-e Zhao, Jia Chen, Yuanzhao Ding, Victor Bochuan Wang, Biqing Bao, Staffan Kjelleberg, Bin Cao, Say Chye Joachim Loo, Lianhui Wang, Wei Huang, Qichun Zhang
ACS APPLIED MATERIALS & INTERFACES
(2015)
Article
Chemistry, Multidisciplinary
Dongliang Yang, Shuwei Zhang, Yanling Hu, Jia Chen, Biqing Bao, Lihui Yuwen, Lixing Weng, Yixiang Cheng, Lianhui Wang
Article
Chemistry, Analytical
Biqing Bao, Yanrui Pan, Bingbing Gu, Jia Chen, Yu Xu, Peng Su, Yunfei Liu, Li Tong, Lianhui Wang
Article
Nanoscience & Nanotechnology
Biqing Bao, Mingfeng Ma, Jia Chen, Lihui Yuwen, Lixing Weng, Quli Fan, Wei Huang, Lianhui Wang
ACS APPLIED MATERIALS & INTERFACES
(2014)
Article
Nanoscience & Nanotechnology
Jia Chen, Jingyang Shan, Yu Xu, Peng Su, Li Tong, Lihui Yuwen, Lixing Weng, Biqing Bao, Lianhui Wang
ACS APPLIED MATERIALS & INTERFACES
(2018)
Article
Chemistry, Analytical
Biqing Bao, Peng Su, Jin Zhu, Jia Chen, Yu Xu, Bingbing Gu, Yunfei Liu, Lianhui Wang
Article
Chemistry, Multidisciplinary
Yu Xu, Jia Chen, Li Tong, Peng Su, Yunfei Liu, Bingbing Gu, Biqing Bao, Lianhui Wang
JOURNAL OF CONTROLLED RELEASE
(2019)
Article
Biophysics
Biqing Bao, Zhenyuan Yang, Yunfei Liu, Yu Xu, Bingbing Gu, Jia Chen, Peng Su, Li Tong, Lianhui Wang
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Chemistry, Physical
Kennedy S. Chen, Lin Qi, Jia Chen, Ruibing Wang, Hua-Zhong Yu
Summary: In this study, alkyne-functionalized CB[7] was successfully immobilized on gold surfaces. The resulting CB[7]-functionalized SAMs showed high stability and uniformity. The study revealed that surface-bound CB[7] exhibits strong host-guest binding towards both cationic and neutral ferrocene derivatives, as well as comparable binding stability towards anionic ferrocene derivatives.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Nanoscience & Nanotechnology
Jia Chen, Qiaoxian Huang, Qingfu Wang, Yuanfu Ding, Siyu Lu, Lian-Hui Wang, Shengke Li, Ruibing Wang
Summary: This study presents a novel bioluminescent probe, based on SLA NPs, for deep-tissue inflammation imaging, offering enhanced imaging performance and biocompatibility.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Jia Chen, Shengke Li, Zeyu Wang, Yating Pan, Jianwen Wei, Siyu Lu, Qing-Wen Zhang, Lian-Hui Wang, Ruibing Wang
Summary: This study presents an AIE photosensitizer functionalized CB[7], AIECB[7], which can self-assemble into nanoaggregates and fine-tune fluorescence and singlet oxygen generation. Additionally, by loading oxaliplatin and hypoxia-activatable prodrug banoxantrone (AQ4N) into AIECB[7] nanoaggregates, synergistic antitumor effects were demonstrated, highlighting the versatile theranostic applications of this supramolecular system.
Article
Materials Science, Biomaterials
Jia Chen, Yunxiao Cui, Kewei Song, Tianqi Liu, Luyao Zhou, Biqing Bao, Ruibing Wang, Lianhui Wang
Summary: The study designed and synthesized a novel photosensitizer based on boron dipyrromethene (BODIPY) for simultaneous photodynamic therapy (PDT) and photothermal therapy (PTT), with a synergistic effect to enhance the antitumor efficiency. The hybrid photosensitizer showed improved phototherapeutic outcome compared to its counterpart, offering guidance for the development of balanced NIR nanoagents for cancer therapeutic efficacy in the future.
BIOMATERIALS SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Jia Chen, Yu Xu, Yu Gao, Dongliang Yang, Fei Wang, Lei Zhang, Biqing Bao, Lianhui Wang
ACS APPLIED MATERIALS & INTERFACES
(2018)