From classical mutagenesis to nuclease-based breeding - directing natural DNA repair for a natural end-product

Precise Editing of a Target Base in the Rice Genome Using a Modified CRISPR/Cas9 System

(2017) Yuming Lu et al.   Molecular Plant

Generation of targeted mutant rice using a CRISPR-Cpf1 system

(2017) Rongfang Xu et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

Origins of Programmable Nucleases for Genome Engineering

(2016) Srinivasan Chandrasegaran et al.   JOURNAL OF MOLECULAR BIOLOGY

CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications

(2016) Xingliang Ma et al.   Molecular Plant

Gene-edited CRISPR mushroom escapes US regulation

(2016) Emily Waltz   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

CRISPR-edited crops free to enter market, skip regulation

(2016) Emily Waltz   NATURE BIOTECHNOLOGY

Precision Targeted Mutagenesis via Cas9 Paired Nickases in Rice

(2016) Masafumi Mikami et al.   PLANT AND CELL PHYSIOLOGY

Regulatory hurdles for genome editing: process- vs. product-based approaches in different regulatory contexts

(2016) Thorben Sprink et al.   PLANT CELL REPORTS

Homology-based double-strand break-induced genome engineering in plants

(2016) Jeannette Steinert et al.   PLANT CELL REPORTS

Site-directed mutagenesis in Petunia × hybrida protoplast system using direct delivery of purified recombinant Cas9 ribonucleoproteins

(2016) Saminathan Subburaj et al.   PLANT CELL REPORTS

Using CRISPR/Cas in three dimensions: towards synthetic plant genomes, transcriptomes and epigenomes

(2016) Holger Puchta   PLANT JOURNAL

Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas

(2016) Simon Schiml et al.   Plant Methods

Oligonucleotide-Mediated Genome Editing Provides Precision and Function to Engineered Nucleases and Antibiotics in Plants

(2016) Noel J. Sauer et al.   PLANT PHYSIOLOGY

The CRISPR-Cas9 technology: Closer to the ultimate toolkit for targeted genome editing

(2016) Francis Quétier   PLANT SCIENCE

Targeted Mutagenesis in Plant Cells through Transformation of Sequence-Specific Nuclease mRNA

(2016) Thomas J. Stoddard et al.   PLoS One

Repair of adjacent single-strand breaks is often accompanied by the formation of tandem sequence duplications in plant genomes

(2016) Simon Schiml et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

(2016) K. Nishida et al.   SCIENCE

CRISPR/Cas9: an advanced tool for editing plant genomes

(2016) Milan Kumar Samanta et al.   TRANSGENIC RESEARCH

Fine-Tuning Next-Generation Genome Editing Tools

(2016) Chidananda Nagamangala Kanchiswamy et al.   TRENDS IN BIOTECHNOLOGY

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

(2016) Sergei Svitashev et al.   Nature Communications

Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

(2016) Yi Zhang et al.   Nature Communications

Highly specific targeted mutagenesis in plants using Staphylococcus aureus Cas9

(2016) Hidetaka Kaya et al.   Scientific Reports

Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida

(2016) Akira Endo et al.   Scientific Reports

The CRISPR/Cas9 system for plant genome editing and beyond

(2015) Luisa Bortesi et al.   BIOTECHNOLOGY ADVANCES

Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System

(2015) Zahir Ali et al.   Molecular Plant

Non-transgenic Plant Genome Editing Using Purified Sequence-Specific Nucleases

(2015) Song Luo et al.   Molecular Plant

USDA approves next-generation GM potato

(2015) Emily Waltz   NATURE BIOTECHNOLOGY

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

Oligonucleotide-directed mutagenesis for precision gene editing

(2015) Noel J. Sauer et al.   PLANT BIOTECHNOLOGY JOURNAL

The Regulatory Status of Genome-edited Crops

(2015) Jeffrey D. Wolt et al.   PLANT BIOTECHNOLOGY JOURNAL

Highly efficient heritable plant genome engineering using Cas9 orthologues fromStreptococcus thermophilusandStaphylococcus aureus

(2015) Jeannette Steinert et al.   PLANT JOURNAL

CRISPR/Cas9-mediated genome editing and gene replacement in plants: Transitioning from lab to field

(2015) Scott M. Schaeffer et al.   PLANT SCIENCE

An Ethylmethane Sulfonate Mutant Resource in Pre-Green Revolution Hexaploid Wheat

(2015) Amandeep K. Dhaliwal et al.   PLoS One

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

(2014) Hui-Li Xing et al.   BMC PLANT BIOLOGY

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

Multigene Knockout Utilizing Off-Target Mutations of the CRISPR/Cas9 System in Rice

(2014) Masaki Endo et al.   PLANT AND CELL PHYSIOLOGY

Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family

(2014) William Haun et al.   PLANT BIOTECHNOLOGY JOURNAL

DNA Replicons for Plant Genome Engineering

(2014) N. J. Baltes et al.   PLANT CELL

The CRISPR/Cas system can be used as nuclease forin plantagene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny

(2014) Simon Schiml et al.   PLANT JOURNAL

Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering inArabidopsis thaliana

(2014) Friedrich Fauser et al.   PLANT JOURNAL

Targeted Genome Editing of Sweet Orange Using Cas9/sgRNA

(2014) Hongge Jia et al.   PLoS One

Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis

(2014) Z. Feng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Precision Genome Engineering and Agriculture: Opportunities and Regulatory Challenges

(2014) Daniel F. Voytas et al.   PLOS BIOLOGY

Plant Genome Engineering with Sequence-Specific Nucleases

(2013) Daniel F. Voytas   Annual Review of Plant Biology

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Gene targeting in plants: 25 years later

(2013) Holger Puchta et al.   INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

(2013) Vladimir Nekrasov et al.   NATURE BIOTECHNOLOGY

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

Comparative regulatory approaches for groups of new plant breeding techniques

(2013) Maria Lusser et al.   New Biotechnology

Engineering nucleases for gene targeting: safety and regulatory considerations

(2013) Katia Pauwels et al.   New Biotechnology

Targeted molecular trait stacking in cotton through targeted double-strand break induction

(2013) Kathleen D'Halluin et al.   PLANT BIOTECHNOLOGY JOURNAL

Synthetic nucleases for genome engineering in plants: prospects for a bright future

(2013) Holger Puchta et al.   PLANT JOURNAL

Precise plant breeding using new genome editing techniques: opportunities, safety and regulation in the EU

(2013) Frank Hartung et al.   PLANT JOURNAL

Nonhomologous End Joining-Mediated Gene Replacement in Plant Cells

(2013) D. M. Weinthal et al.   PLANT PHYSIOLOGY

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Site-directed nucleases: a paradigm shift in predictable, knowledge-based plant breeding

(2013) Nancy Podevin et al.   TRENDS IN BIOTECHNOLOGY

RNA-Guided Genome Editing for Target Gene Mutations in Wheat

(2013) Santosh Kumar Upadhyay et al.   G3-Genes Genomes Genetics

Transgenic or not? No simple answer!

(2012) Nancy Podevin et al.   EMBO REPORTS

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

Transcription activator-like (TAL) effectors targetingOsSWEETgenes enhance virulence on diverse rice (Oryza sativa) varieties when expressed individually in a TAL effector-deficient strain ofXanthomonas oryzae

(2012) Valérie Verdier et al.   NEW PHYTOLOGIST

In planta gene targeting

(2012) F. Fauser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Targeted chromosomal duplications and inversions in the human genome using zinc finger nucleases

(2011) H. J. Lee et al.   GENOME RESEARCH

Induction and repair of DNA double strand breaks: The increasing spectrum of non-homologous end joining pathways

(2011) Emil Mladenov et al.   MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS

Pathways to meiotic recombination in Arabidopsis thaliana

(2011) Kim Osman et al.   NEW PHYTOLOGIST

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

THE GENETICAL EFFECTS OF MUSTARD GAS SUBSTANCES AND NEUTRONS

(2010) Å. GUSTAFSSON et al.   HEREDITAS

Zinc finger nuclease-mediated transgene deletion

(2010) Joseph F. Petolino et al.   PLANT MOLECULAR BIOLOGY

The I-CreI meganuclease and its engineered derivatives: applications from cell modification to gene therapy

(2010) S. Arnould et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Precise genome modification in the crop species Zea mays using zinc-finger nucleases

(2009) Vipula K. Shukla et al.   NATURE

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

A Simple Cipher Governs DNA Recognition by TAL Effectors

(2009) M. J. Moscou et al.   SCIENCE