Article
Agriculture, Dairy & Animal Science
Telisson Ribeiro Goncalves, Jose Renaldo Vilar da Silva Filho, Gilmar Amaro Pereira, Tarcisio Martins Meira, Moema Kelly Nogueira de Sa, Glayciane Costa Gois, Crislane de Souza Silva, Fleming Sena Campos, Sandra Mari Yamamoto, Gherman Garcia Leal de Araujo
Summary: This study evaluated the effects of diets containing vitiviniculture waste on intake, digestibility, water balance, nitrogen balance, ingestive behavior, performance, and carcass traits of lambs. The results showed that feeding lambs with diets containing 216 g/kg cactus pear and 144 g/kg vitiviniculture waste resulted in higher intake and digestibility.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2024)
Article
Agronomy
Arleen Rodriguez-Declet, Antonio Castro-Marin, Luca Moretti Conti, Alessandra Lombini, Fabio Chinnici, Adamo Domenico Rombola
Summary: Volatile organic compounds (VOCs) are important for communication among plants and other organisms. This study identified 25 VOCs emitted by the grapevine cv. Isabella and revealed differences in volatile profiles between diurnal and nocturnal treatments. The presence of previously unreported compounds and their role in plant defense highlight the importance of this research in understanding pathogen tolerance mechanisms and exploring innovative cropping systems.
Article
Horticulture
Wenxin Li, Chang He, Hongli Wei, Jiakang Qian, Jiannan Xie, Zhiqian Li, Xianbo Zheng, Bin Tan, Jidong Li, Jun Cheng, Wei Wang, Xia Ye, Jiancan Feng
Summary: This study provides a comprehensive analysis of the grapevine pectin lyase gene family and reveals the important role of VvPL11 in fruit softening.
Article
Plant Sciences
Jay Wason, Martin Bouda, Eric F. Lee, Andrew J. McElrone, Ronald J. Phillips, Kenneth A. Shackel, Mark A. Matthews, Craig Brodersen
Summary: This study reconstructed and simulated grapevine xylem networks, finding that the mean pit area per intervessel connection was constant and short segments failed to capture complete network connectivity. Simulations showed that xylem network organization can increase stem resistance to embolism spread.
Article
Plant Sciences
Hilal Betul Kaya, Yildiz Dilli, Tulay Oncu-Oner, Akay uenal
Summary: Grapevine has been a significant perennial crop for thousands of years, with economic importance worldwide. Turkish grapevine cultivation dates back to ancient times, and this study provided comprehensive information on the genetic diversity and population structure of Turkish grapevine genotypes, which is crucial for genomic-assisted breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Physics, Applied
Jong Woo Kim, Mathew J. Cherukara, Ashish Tripathi, Zhang Jiang, Jin Wang
Summary: Coherent surface scattering imaging requires a mathematical conversion to visualize specimen structures, which can now be predicted using a deep learning neural network model to instantly obtain results from scattering patterns. This is highly beneficial for both experimental effectiveness and data analysis.
APPLIED PHYSICS LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Cen Chen, Ziyuan Fang, Zhen Huang
Summary: Crystallization phasing and obtaining high-quality crystals are major challenges for X-ray crystallographic analysis of nucleic acids. In this study, the authors addressed these challenges by incorporating selenium atoms into DNA duplexes, which resulted in larger and higher quality crystals compared to standard DNA crystals. These findings provide a simple strategy to overcome crystallization challenges in nucleic acid crystallography.
CRYSTAL GROWTH & DESIGN
(2022)
Article
Plant Sciences
Brian Ingel, Clarissa Reyes, Melanie Massonnet, Bailey Boudreau, Yuling Sun, Qiang Sun, Andrew J. McElrone, Dario Cantu, M. Caroline Roper
Summary: Pierce's disease in grapevines is caused by the bacterial pathogen Xylella fastidiosa. The disease induces extensive tylose-mediated vessel occlusions, particularly in susceptible V. vinifera, with significant transcriptomic reprogramming occurring during early stages. Genes associated with tylose formation and drought stress were up-regulated, while photosynthesis and carbon fixation genes were down-regulated, correlating with starch depletion in ray cells and tylose synthesis in vessels.
MOLECULAR PLANT PATHOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Noriyoshi Manabe, Yoshiki Yamaguchi
Summary: Beta-glucans are components of fungal and plant cell walls, and are recognized as non-self-components in the host defense system. Their long chains can form a triple helix structure, potentially affecting interactions with beta-glucan-binding proteins. While details of beta-glucan binding and receptor signaling remain unclear, X-ray crystallography and NMR analyses have provided insights into their structure and interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Giovanni Bortolami, Elena Farolfi, Eric Badel, Regis Burlett, Herve Cochard, Nathalie Ferrer, Andrew King, Laurent J. Lamarque, Pascal Lecomte, Marie Marchesseau-Marchal, Jerome Pouzoulet, Jose M. Torres-Ruiz, Santiago Trueba, Sylvain Delzon, Gregory A. Gambetta, Chloe E. L. Delmas
Summary: The study found that during esca, a grapevine disease, the stem hydraulic conductivity was affected, with severe symptoms showing a high occurrence of xylem occlusions and subsequent loss of stem hydraulic conductivity, while asymptomatic shoots did not show these effects. Tyloses only occurred when leaf symptoms appeared and resulted in significant loss of stem hydraulic conductivity.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Multidisciplinary Sciences
Akimitsu Higuchi, Wataru Shihoya, Masae Konno, Tatsuya Ikuta, Hideki Kandori, Keiichi Inoue, Osamu Nureki
Summary: SzRs, a newly identified rhodopsin family in Asgard archaea, exhibit unique kinetic behaviors in inward H+ release compared to other H+ pumps. The crystal structure of SzR AM_5_00977 revealed that key residues and structural features are different from other microbial rhodopsins, suggesting a distinct mechanism for inward H+ release.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Hai Li, Chia-Ying Huang, Elena G. Govorunova, Oleg A. Sineshchekov, Adrian Yi, Kenneth J. Rothschild, Meitian Wang, Lei Zheng, John L. Spudich
Summary: The crystal structure of bromide-bound GtACR1 reveals structural changes that relax the constrictions of the channel and involve a novel salt-bridge switch mechanism. These findings suggest that substrate binding induces a transition from an inactivated state to a pre-activated state in the dark, facilitating channel opening.
Article
Chemistry, Multidisciplinary
Matic Lozinsek, Helene P. A. Mercier, Gary J. Schrobilgen
Summary: The coordination chemistry of KrF2 is limited compared to XeF2, but in this study, new mixed krypton/xenon compounds were synthesized where KrF2 acts as a ligand coordinating to xenon atoms. This extends the XeF2-KrF2 analogy and introduces a new class of coordination compound.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Sunbin Deng, Jiaxi Cai, Stephen C. Harrison, Huilin Zhou, Stephen M. Hinshaw
Summary: Successful mitosis relies on the correct attachment of chromosomes to microtubules. The kinetochore plays a crucial role in this process by recognizing specific chromatin and ensuring the formation of only one kinetochore per chromatid at the correct location. The crystal structure of the Cse4 peptide bound to the inner kinetochore Okp1-Ame1 complex provides insights into centromere recognition and the organization of the inner kinetochore.
Article
Chemistry, Applied
Rukhsana J. J. Wani, Altaf Hussain Pandith, Arshad Hussain, Taniya Manzoor, Maryam Fatima, Mohd Nadeem Bukhari
Summary: The new eight-coordinate thorium(IV) complex was synthesized by coprecipitation method using thorium nitrate, 1,10-phenanthroline, and 2-hydroxy-5-sulfobenzoic acid. The complex was characterized by various techniques and found to be amorphous, diamagnetic, air stable, and water soluble. The FTIR studies confirmed the coordination of thorium with sulfosalicylic acid, phenanthroline, and nitrate ions through oxygen and nitrogen atoms.
APPLIED ORGANOMETALLIC CHEMISTRY
(2023)
Review
Cardiac & Cardiovascular Systems
Sina Safabakhsh, Pankaj Panwar, Scott Barichello, Sarabjit S. Sangha, Paul J. Hanson, Filip Van Petegem, Zachary Laksman
Summary: Atrial fibrillation (AF) is the most common arrhythmia worldwide and is associated with increased morbidity and mortality. The post-translational modification of proteins, especially protein phosphorylation, may explain how risk factors lead to AF. Mass spectrometry (MS) is a common and highly resolute technique for studying protein phosphorylation, and future research utilizing MS technology aims to better understand the role of phosphorylation in the pathophysiology of AF.
CARDIOVASCULAR RESEARCH
(2022)
Article
Plant Sciences
Jan Xue, Pallinti Purushotham, Justin F. Acheson, Ruoya Ho, Jochen Zimmer, Ciaran McFarlane, Filip Van Petegem, Patrick T. Martone, A. Lacey Samuels
Summary: This study functionally characterized cellulose synthases (CESAs) from the coralline alga Calliarthron, providing evidence validating the sequence annotation of red algal CESAs. The relatively low activity of the CESA, partial complementation in Arabidopsis, and presence of unique protein domains suggest functional differences between algal and land plant CESAs.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Physiology
Kellie A. Woll, Filip Van Petegem
Summary: Ca2+-release channels are membrane proteins that control the release of Ca2+ from the endoplasmic and sarcoplasmic reticulum. They are activated by cytosolic Ca2+ and have a common architecture, with additional modules in the cytosolic region for ryanodine receptors (RyRs). Their regulation involves the binding of proteins and small molecules, with major triggers including IP3 and membrane depolarization. Electron microscopic studies have provided valuable insights into their structure and mechanisms, including the binding of auxiliary proteins, regulation of channel opening, and disease-associated mutations.
PHYSIOLOGICAL REVIEWS
(2022)
Article
Biotechnology & Applied Microbiology
Julie M. Grondin, Guillaume Dejean, Filip Van Petegem, Harry Brumer
Summary: This study reveals the complementary roles of syntenic and nonsyntenic XyGUL in B. uniformis in conferring growth on a variety of XyG-derived substrates, providing evidence of glycan-binding protein microadaptation within a single species. These data serve as a comprehensive overview of the binding capacities of the SGBPs from a nonsyntenic B. uniformis XyGUL and will inform future studies on the roles of complementary loci in glycan targeting by key HGM species.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jingyan Zhang, Daniel P. Singh, Christopher Y. Ko, Roman Nikolaienko, Siobhan M. Wong King Yuen, Jacob A. Schwarz, Levy M. Treinen, Ching-Chieh Tung, Kaja Rozman, Bengt Svensson, Courtney C. Aldrich, Aleksey Zima, David D. Thomas, Donald M. Bers, Bradley S. Launikonis, Filip Van Petegem, Razvan L. Cornea
Summary: This study used NTR biosensors to screen for potential therapeutic compounds that modulate RyR channels. Several novel inhibitors and selective activators were identified, as well as a potential enhancer of excitation-contraction coupling. Additionally, further investigation of an FDA-approved antibiotic, fusidic acid, showed its ability to inhibit RyR leak but with cautionary observations of arrhythmogenic calcium transients in intact cardiomyocytes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Daniela Rossi, Stefania Lorenzini, Enrico Pierantozzi, Filip Van Petegem, David Osamwonuyi Amadsun, Vincenzo Sorrentino
Summary: Junctin is a transmembrane protein that plays a role in striated muscles by interacting with calsequestrin and the ryanodine receptor. Our study found that junctin and calsequestrin co-localize in the endoplasmic reticulum and triads, and identified specific regions of junctin that can bind calsequestrin and the RyR. Deletion of these regions affects junctin's localization at the junctional SR.
JOURNAL OF CELL SCIENCE
(2022)
Article
Chemistry, Organic
Nicholas G. S. McGregor, Chi-Lin Kuo, Thomas J. M. Beenakker, Chun-Sing Wong, Wendy A. Offen, Zachary Armstrong, Bogdan Florea, Jeroen D. C. Codee, Herman S. Overkleeft, Johannes M. F. G. Aerts, Gideon J. Davies
Summary: Researchers have synthesized beta-manno-configured cyclophellitol and its analogues for detecting exo-beta-mannosidases. These probes selectively label exo-beta-mannosidases from different GH families and structural studies confirm their mechanism of action.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Biophysics
Leo C. T. Ng, Yue Xian Li, Filip Van Petegem, Eric A. Accili
Summary: An increasing number of nonsynonymous mutations in the HCN4 channel gene are associated with disease, but the impact of these mutations on channel structure and function, and the molecular mechanisms underlying the disease, are not well understood. In this study, the S672R mutation in the cyclic nucleotide-binding domain of the channel was investigated. It was found that the mutant channel has weaker binding to cAMP and cGMP, which may contribute to the reduced resting heart rate observed in the affected family.
BIOPHYSICAL JOURNAL
(2022)
Article
Clinical Neurology
Thomas N. O'Connor, Luuk R. van den Bersselaar, Yu Seby Chen, Stefan Nicolau, Brentney Simon, Andrew Huseth, Joshua J. Todd, Filip Van Petegem, Anna Sarkozy, Michael F. Goldberg, Nicol C. Voermans, Robert T. Dirksen
JOURNAL OF NEUROMUSCULAR DISEASES
(2023)
Review
Chemistry, Multidisciplinary
Evolene Desligniere, Amber Rolland, Eduard H. T. M. Ebberink, Victor Yin, Albert J. R. Heck
Summary: Native mass spectrometry is widely used for determining the mass of intact proteins and their biomolecular assemblies. However, it can be challenging for heterogeneous protein complexes. In 2012, an Orbitrap-based mass analyzer with extended mass range was introduced, enabling high-resolution mass spectra of large protein assemblies and single ion measurements. This led to the development of single-molecule Orbitrap-based charge detection mass spectrometry in 2020, which has opened doors for innovative research in various systems.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Omid Haji-Ghassemi, Yu Seby Chen, Kellie Woll, Georgina B. Gurrola, Carmen R. Valdivia, Wenxuan Cai, Songhua Li, Hector H. Valdivia, Filip Van Petegem
Summary: Calcins are peptides from scorpion venom that can cross cell membranes and target intracellular ion channels called Ryanodine Receptors (RyR). A study using cryo-electron microscopy found that imperacalcin, a type of calcin, binds to RyR and opens the channel pore, resulting in asymmetry and extended ion conduction pathways. Phosphorylation of imperacalcin by protein kinase A prevents its binding to RyR, highlighting the role of posttranslational modifications in determining the fate of a natural toxin. The structure of imperacalcin provides a template for developing analogs with potential to treat RyR-related disorders.
Meeting Abstract
Biochemistry & Molecular Biology
Spencer Cholak, James Saville, Xing Zhu, Alison Berezuk, Omid Haji-Ghassemi, Katharine Tuttle, Filip Van Petegem, Sriram Subramaniam
Meeting Abstract
Anesthesiology
Thomas T. Joseph, Weiming Bu, Kellie Woll, Omid Haji-Ghassemi, Filip van Petegem, Grace Brannigan, Roderic G. Eckenhoff
ANESTHESIA AND ANALGESIA
(2022)
Meeting Abstract
Biophysics
Yu S. Chen, Kellie A. Woll, Omid Haji-Ghassemi, Filip Van Petegem
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Zheng Fang Yang, Pankaj Panwar, Ciaran McFarlane, Marta Campiglio, Wietske E. Tuinte, Filip Van Petegem
BIOPHYSICAL JOURNAL
(2022)