New traits in crops produced by genome editing techniques based on deletions

Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System

(2017) Jingying Li et al.   Molecular Plant

Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing

(2017) Wen Zhi Jiang et al.   PLANT BIOTECHNOLOGY JOURNAL

Targeted modification of plant genomes for precision crop breeding

(2016) Julia Hilscher et al.   Biotechnology Journal

CRISPR/Cas9: a promising way to exploit genetic variation in plants

(2016) Reema Rani et al.   BIOTECHNOLOGY LETTERS

Superheroes and masterminds of plant domestication

(2016) Natalia E. Martínez-Ainsworth et al.   COMPTES RENDUS BIOLOGIES

Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq

(2016) Daesik Kim et al.   GENOME RESEARCH

DNA Breaks and End Resection Measured Genome-wide by End Sequencing

(2016) Andres Canela et al.   MOLECULAR CELL

Engineering of CRISPR/Cas9-mediated potyvirus resistance in transgene-freeArabidopsisplants

(2016) Douglas E. Pyott et al.   MOLECULAR PLANT PATHOLOGY

Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology

(2016) Jeyabharathy Chandrasekaran et al.   MOLECULAR PLANT PATHOLOGY

Reducing phosphorus accumulation in rice grains with an impaired transporter in the node

(2016) Naoki Yamaji et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Will Europe toast GM wheat for gluten sufferers?

(2016) Lucas Laursen   NATURE BIOTECHNOLOGY

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells

(2016) Daesik Kim et al.   NATURE BIOTECHNOLOGY

Subgenome parallel selection is associated with morphotype diversification and convergent crop domestication in Brassica rapa and Brassica oleracea

(2016) Feng Cheng et al.   NATURE GENETICS

Advancing Crop Transformation in the Era of Genome Editing

(2016) Fredy Altpeter et al.   PLANT CELL

Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts

(2016) Mariette Andersson et al.   PLANT CELL REPORTS

CRISPR/Cas9 for plant genome editing: accomplishments, problems and prospects

(2016) Joseph W. Paul et al.   PLANT CELL REPORTS

Applications of CRISPR/Cas9 technology for targeted mutagenesis, gene replacement and stacking of genes in higher plants

(2016) Ming Luo et al.   PLANT CELL REPORTS

Regulatory hurdles for genome editing: process- vs. product-based approaches in different regulatory contexts

(2016) Thorben Sprink et al.   PLANT CELL REPORTS

Site-directed mutagenesis in Petunia × hybrida protoplast system using direct delivery of purified recombinant Cas9 ribonucleoproteins

(2016) Saminathan Subburaj et al.   PLANT CELL REPORTS

Gene targeting and transgene stacking using intra genomic homologous recombination in plants

(2016) Sandeep Kumar et al.   Plant Methods

Silencing of six susceptibility genes results in potato late blight resistance

(2016) Kaile Sun et al.   TRANSGENIC RESEARCH

Opportunities for Products of New Plant Breeding Techniques

(2016) Jan G. Schaart et al.   TRENDS IN PLANT SCIENCE

DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

(2016) Kwangryul Baek et al.   Scientific Reports

Targeted modification of plant genomes for precision crop breeding

(2016) Julia Hilscher et al.   Biotechnology Journal

Genome-Wide Study of the Tomato SlMLO Gene Family and Its Functional Characterization in Response to the Powdery Mildew Fungus Oidium neolycopersici

(2016) Zheng Zheng et al.   Frontiers in Plant Science

Genome-Wide Assessment of Efficiency and Specificity in CRISPR/Cas9 Mediated Multiple Site Targeting in Arabidopsis

(2016) Brenda A. Peterson et al.   PLoS One

First successful reduction of clinical allergenicity of food by genetic modification:Mal d 1-silenced apples cause fewer allergy symptoms than the wild-type cultivar

(2015) A. E. J. Dubois et al.   ALLERGY

Principle and application of plant mutagenesis in crop improvement: a review

(2015) Yusuff Oladosu et al.   BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT

Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing

(2015) Mai Tsuda et al.   BMC GENOMICS

Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System

(2015) Zahir Ali et al.   Molecular Plant

High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System

(2015) Liuhua Yan et al.   Molecular Plant

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

(2015) Daesik Kim et al.   NATURE METHODS

Exploiting SNPs for biallelic CRISPR mutations in the outcrossing woody perennialPopulusreveals 4-coumarate:CoA ligase specificity and redundancy

(2015) Xiaohong Zhou et al.   NEW PHYTOLOGIST

Oligonucleotide-directed mutagenesis for precision gene editing

(2015) Noel J. Sauer et al.   PLANT BIOTECHNOLOGY JOURNAL

Improving cold storage and processing traits in potato through targeted gene knockout

(2015) Benjamin M. Clasen et al.   PLANT BIOTECHNOLOGY JOURNAL

Creation of fragrant rice by targeted knockout of theOsBADH2gene using TALEN technology

(2015) Qiwei Shan et al.   PLANT BIOTECHNOLOGY JOURNAL

Targeting of prolamins by RNAi in bread wheat: effectiveness of seven silencing-fragment combinations for obtaining lines devoid of coeliac disease epitopes from highly immunogenic gliadins

(2015) Francisco Barro et al.   PLANT BIOTECHNOLOGY JOURNAL

Multiplexed, targeted gene editing inNicotiana benthamianafor glyco-engineering and monoclonal antibody production

(2015) Jin Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Silencing of vacuolar invertase and asparagine synthetase genes and its impact on acrylamide formation of fried potato products

(2015) Xiaobiao Zhu et al.   PLANT BIOTECHNOLOGY JOURNAL

Highly efficient heritable plant genome engineering using Cas9 orthologues fromStreptococcus thermophilusandStaphylococcus aureus

(2015) Jeannette Steinert et al.   PLANT JOURNAL

Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA

(2015) Sergei Svitashev et al.   PLANT PHYSIOLOGY

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Down-regulation of Arabidopsis DND1 orthologs in potato and tomato leads to broad-spectrum resistance to late blight and powdery mildew

(2015) Kaile Sun et al.   TRANSGENIC RESEARCH

Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

(2015) Zhi-Ping Wang et al.   GENOME BIOLOGY

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Magical mystery tour: MLO proteins in plant immunity and beyond

(2014) Johanna Acevedo-Garcia et al.   NEW PHYTOLOGIST

Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice

(2014) Huanbin Zhou et al.   NUCLEIC ACIDS RESEARCH

Adaptation to the Local Environment by Modifications of the Photoperiod Response in Crops

(2014) Norihito Nakamichi   PLANT AND CELL PHYSIOLOGY

Multigene Knockout Utilizing Off-Target Mutations of the CRISPR/Cas9 System in Rice

(2014) Masaki Endo et al.   PLANT AND CELL PHYSIOLOGY

Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family

(2014) William Haun et al.   PLANT BIOTECHNOLOGY JOURNAL

The CRISPR/Cas system can be used as nuclease forin plantagene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny

(2014) Simon Schiml et al.   PLANT JOURNAL

A qRT-PCR assay for the expression of all Mal d 1 isoallergen genes

(2013) Giulia Pagliarani et al.   BMC PLANT BIOLOGY

Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System

(2013) Zhen Liang et al.   Journal of Genetics and Genomics

Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing

(2013) Nicola Crosetto et al.   NATURE METHODS

TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants

(2013) Toni Wendt et al.   PLANT MOLECULAR BIOLOGY

Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase

(2013) R. Van Acker et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Celiac disease T-cell epitopes from gamma-gliadins: immunoreactivity depends on the genome of origin, transcript frequency, and flanking protein variation

(2012) Elma MJ Salentijn et al.   BMC GENOMICS

Deployment of new biotechnologies in plant breeding

(2012) Maria Lusser et al.   NATURE BIOTECHNOLOGY

High-efficiency TALEN-based gene editing produces disease-resistant rice

(2012) Ting Li et al.   NATURE BIOTECHNOLOGY

Removing celiac disease-related gluten proteins from bread wheat while retaining technological properties: a study with Chinese Spring deletion lines

(2009) Hetty C van den Broeck et al.   BMC PLANT BIOLOGY

A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat

(2009) Cristobal Uauy et al.   BMC PLANT BIOLOGY

Loss of susceptibility as a novel breeding strategy for durable and broad-spectrum resistance

(2009) Stefano Pavan et al.   MOLECULAR BREEDING

Alleviating peanut allergy using genetic engineering: the silencing of the immunodominant allergen Ara h 2 leads to its significant reduction and a decrease in peanut allergenicity

(2007) Hortense W. Dodo et al.   PLANT BIOTECHNOLOGY JOURNAL