Article
Oncology
Yiwei He, Gang Wang, Qian Wang, Zheng Zhao, Lu Gan, Shirong Yang, Yongxing Wang, Shanshan Guo, Jiaze An, Jian Zhang, Zhaohui Zhang, Feng Zhou
Summary: This study suggests that NPAS2 gene polymorphisms may serve as an independent prognostic marker for NSCLC patients. Specifically, SNP rs2305158 is significantly associated with NSCLC overall survival, and lymph node metastasis is significantly correlated with increased risk of death in patients with the homozygous wildtype (WW) genotype of rs2305158. Additionally, SNP rs2305158 interacts with lymph node metastasis stage and modulates the effects of lymph node metastasis stage on NSCLC death.
Article
Biochemistry & Molecular Biology
Justyna Basak, Danuta Piotrzkowska, Ireneusz Majsterek, Ewa Kucharska
Summary: Multiple sclerosis (MS) is an autoimmune disorder of the central nervous system with no cure and unclear etiology. This study aimed to identify SNPs associated with an increased risk of developing MS. The findings suggest that the GG AGO1 rs636832 and AA GEMIN4 rs7813 genotypes are associated with an increased risk of MS.
Article
Oncology
Xianghan Li, Yiran Zou, Teng Li, Thomas K. F. Wong, Ryan T. Bushey, Michael J. Campa, Elizabeth B. Gottlin, Hongliang Liu, Qingyi Wei, Allen Rodrigo, Edward F. Patz
Summary: SNPs identified in the CLPP and M1AP genes may be useful in risk prediction models for lung cancer, and the previously established association of the CLPP gene with cancer progression lends relevance to our findings.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, Research & Experimental
Yuan Gu, Gianni Pais, Vivien Becker, Christina Korbel, Emmanuel Ampofo, Elke Ebert, Johannes Hohneck, Nicole Ludwig, Eckart Meese, Rainer M. Bohle, Yingjun Zhao, Michael D. Menger, Matthias W. Laschke
Summary: The research demonstrates that miR-22 is highly expressed in ECs and significantly downregulated in NSCLC tissues. Endothelial miR-22 acts as an angiogenesis inhibitor by targeting sirtuin 1 and FGFR1 to inhibit ECs' angiogenic activities, thus suppressing NSCLC growth.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Oncology
Anni Qiu, Huiwen Xu, Liping Mao, Buyun Xu, Xiaoyu Fu, Jingwen Cheng, Rongrong Zhao, Zhounan Cheng, Xiaoxuan Liu, Jingsheng Xu, Yan Zhou, Yang Dong, Tian Tian, Guangyu Tian, Minjie Chu
Summary: This study identified a novel apaQTL-SNP, rs10138506, which may affect lung adenocarcinoma (LUAD) risk by modulating the 3' UTR length of CHURC1. Further experiments showed that rs10138506, in perfect LD with rs1127968, can mediate changes in the lengths of the 3' UTR of CHURC1 isoforms by choosing different poly(A) sites.
Article
Multidisciplinary Sciences
Karolina Hanna Prazanowska, Su Bin Lim
Summary: As the importance of single-cell RNA sequencing (scRNA-seq) in studying cellular heterogeneity grows, the number of available scRNA-seq datasets has also increased rapidly. However, reusing such data is often difficult due to small cohort size, limited cell types, and insufficient information on cell type classification. Here, we present a large integrated scRNA-seq dataset containing 224,611 cells from human primary non-small cell lung cancer (NSCLC) tumors. We preprocess and integrate seven independent scRNA-seq datasets using an anchor-based approach, with validation using two additional datasets. This integrated data serves as a valuable resource for studying NSCLC transcriptome at the single cell level.
Article
Oncology
Jieyi Long, Tingting Long, Ying Li, Peihong Yuan, Ke Liu, Jiaoyuan Li, Liming Cheng
Summary: The study identified a functional variant rs13064999 contributing to non-small cell lung cancer (NSCLC) susceptibility by integrating ATAC-seq and ChIP-seq data, and validated its regulatory role through population study and biochemical experiments.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Umberto Malapelle, Paola Parente, Francesco Pepe, Martina Concetta Di Micco, Alessandro Russo, Celeste Clemente, Paolo Graziano, Antonio Rossi
Summary: B7-H3, as an immune checkpoint molecule, is overexpressed in tumor tissues and associated with poor prognosis, making it a potential target for cancer immunotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yunxia Ma, Desiree Charlotte Schroeder, Miljana Nenkov, Maryam Noor Rizwan, Mohamed Abubrig, Juergen Sonnemann, Jose M. Murrieta-Coxca, Diana M. Morales-Prieto, Martin Westermann, Nikolaus Gassler, Yuan Chen
Summary: The study suggests that decreased EMP1 expression is significantly associated with increased tumor size in NSCLC, while overexpression of EMP2 can suppress tumor cell growth, migration, and invasion, and affect cell cycle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Bing-qi Hu, Jun-feng Huang, Ke Niu, Jing Zhou, Nan-nan Wang, Yu Liu, Wen Chen
Summary: Dihydroartemisinin (DHA), an active metabolite of artemisinin, shows anticancer activities in non-small cell lung cancer (NSCLC) by suppressing tumor growth and inducing CD8+ T lymphocyte infiltration. It inhibits the expression of B7-H3, which is involved in cell proliferation, migration, and apoptosis, rather than PD-L1. These findings suggest that DHA's antitumor effects in NSCLC may be attributed to B7-H3 blockade, indicating a potential combination with anti-PD-L1/PD-1 immunotherapy.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Oncology
Xingyu Fan, Junfeng Huang, Bingqi Hu, Jing Zhou, Liwen Chen
Summary: This study found that tumor-expressed B7-H3 promotes VM formation by NSCLC cells via the PI3K/AKT signaling pathway, but has no effect on angiogenesis in NSCLC.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Nutrition & Dietetics
Laura Elena Pineda Lancheros, Susana Rojo Tolosa, Jose Maria Galvez Navas, Fernando Martinez Martinez, Almudena Sanchez Martin, Alberto Jimenez Morales, Cristina Perez Ramirez
Summary: This study found an association between the VDR BsmI rs1544410 polymorphism in the vitamin D metabolic pathway and a lower risk of non-small-cell lung cancer.
Article
Oncology
Tatsuya Ogimoto, Hiroaki Ozasa, Hironori Yoshida, Takashi Nomizo, Tomoko Funazo, Hiroshi Yoshida, Kentaro Hashimoto, Kazutaka Hosoya, Masatoshi Yamazoe, Hitomi Ajimizu, Takahiro Tsuji, Yuichi Sakamori, Kiyomitsu Kuninaga, Satoshi Morita, Toyohiro Hirai
Summary: This study investigated the association between CD47 gene polymorphisms and the therapeutic effect of nivolumab in patients with NSCLC. The results showed that the G/G genotype of CD47 polymorphisms was significantly associated with longer progression-free survival and overall survival. Therefore, CD47 gene polymorphisms may serve as a novel predictive biomarker for nivolumab efficacy in advanced NSCLC patients.
Article
Biotechnology & Applied Microbiology
Yushun Bai, Ji Zheng, Lei Cheng, Qi Liu, Genming Zhao, Jingrao Li, Yanzi Gu, Wanghong Xu, Mengyun Wang, Qingyi Wei, Ruoxin Zhang
Summary: This study identified genetic variants in the immune-activation gene set that are associated with overall survival in non-small cell lung cancer patients. Two validated variants were found to potentially affect lung cancer survival by regulating the expression of corresponding genes.
JOURNAL OF GENE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Wenwen Du, Jianjie Zhu, Yuanyuan Zeng, Ting Liu, Yang Zhang, Tingting Cai, Yulong Fu, Weijie Zhang, Ruochen Zhang, Zeyi Liu, Jian-an Huang
Summary: This study demonstrates for the first time that PD-L1 regulates NSCLC cell proliferation through the Gas6/MerTK signaling pathway, and reveals a molecular mechanism by which PD-L1 translocates into the nucleus to promote Gas6 synthesis and activate the MerTK signaling pathway. These findings provide potential implications for PD-L1 targeted immunotherapy in the clinic.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Caicun Zhou, Gongyan Chen, Yunchao Huang, Jianying Zhou, LiZhu Lin, Jifeng Feng, Zhehai Wang, YongQian Shu, Jianhua Shi, Yi Hu, QiMing Wang, Ying Cheng, Fengying Wu, Jianhua Chen, Xiaoyan Lin, Yongsheng Wang, Jianan Huang, Jiuwei Cui, Lejie Cao, Yunpeng Liu, Yiping Zhang, Yueyin Pan, Jun Zhao, LiPing Wang, Jianhua Chang, Qun Chen, Xiubao Ren, Wei Zhang, Yun Fan, Zhiyong He, Jian Fang, Kangsheng Gu, XiaoRong Dong, Faguang Jin, Hongjun Gao, Guangyu An, Cuimin Ding, Xiaodong Jiang, Jianping Xiong, Xiangdong Zhou, Sheng Hu, Ping Lu, Anwen Liu, Shuliang Guo, Jianjin Huang, Chengchu Zhu, Jian Zhao, Beili Gao, Yinglan Chen, Chengping Hu, Jian Zhang, Hongmei Zhang, Hui Zhao, Yanfei Tai, Xinjing Ma, Wei Shi, CameL Study Grp
Summary: The addition of camrelizumab to first-line chemotherapy significantly improves the progression-free survival of patients with advanced nonsquamous NSCLC. It provides long-term survival benefit with manageable toxicity and durable response. These results support the combination of camrelizumab and chemotherapy as a first-line treatment for advanced nonsquamous NSCLC.
JOURNAL OF THORACIC ONCOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Bohang Wu, Reece W. W. Lewis, Guotai Li, Yifan Gao, Bowen Fan, Benjamin Klemm, Jianan Huang, Junyou Wang, Martien A. A. Cohen Stuart, Rienk Eelkema
Summary: By constructing a coacervate hydrogel with a Michael addition-based chemical reaction network (CRN) as a platform, the state of the coacervate materials can be easily controlled by specific chemical signals. The designed pyridine-based ABA triblock copolymer can undergo regulation through quaternization by an allyl acetate electrophile and an amine nucleophile, resulting in gel construction and collapse in the presence of polyanions. The coacervate gels exhibit highly tunable stiffness and gelation times, as well as excellent self-healing ability and injectability with different sized needles, and accelerated degradation induced by chemical signal-induced coacervation disruption. This work represents a first step towards the development of a new class of signal-responsive injectable materials.
Article
Chemistry, Multidisciplinary
Marzia Iarossi, Daniel Darvill, Aliaksandr Hubarevich, Jian-An Huang, Yingqi Zhao, Angela Federica De Fazio, Devin B. O'Neill, Francesco Tantussi, Francesco De Angelis
Summary: Solid-state nanopores can sense biomolecules at the single-molecule level, but limitations and challenges exist in current electrical and optical sensing strategies. In contrast, plasmonic nanoassemblies that support high-density nanopores have shown promising results in capturing analytes and discriminating DNA sequences using surface-enhanced Raman scattering.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Environmental
Lingling Jiang, Jiale Zhang, Hongying Li, Chaoqin He, Ruo Yuan, Yingqi Zhao, Jian-An Huang, Ying Zhuo, Xia Yang
Summary: In this study, a highly efficient SERS substrate Sn1-xCexO2 was synthesized by varying concentrations of Ce ions and introducing oxygen vacancies. The charge transfer pathway and strong interaction between the Sn1-xCexO2 nanoflowers (NFs) and methylene blue (MB) molecules were promoted, resulting in an enhancement factor (EF) as high as 1.80 x 106. This study provides an approach for the preparation of new SERS substrates that can be used in bioanalysis.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Respiratory System
Chuan-cai Xu, Zhi-song He, Wei Lei, Jin-zhou Zhu, Da-guo Zhao, Jin-dan Kong, Yao Wei, Ying Xu, Jian-An Huang
Summary: This study found that patients with breakthrough infections after receiving the COVID-19 vaccine had higher lymphocyte counts, anti-SARS-CoV-2 spike IgG and IgM antibody titers within 60 days of receiving the second dose compared to those with a 60-day or longer interval. Additionally, the IL-6 level was significantly lower in the group with a shorter interval.
CLINICAL RESPIRATORY JOURNAL
(2023)
Review
Food Science & Technology
Meng-ge Tang, Sheng Zhang, Li-gui Xiong, Jing-hui Zhou, Jian-an Huang, Ai-qing Zhao, Zhong-hua Liu, Ai-ling Liu
Summary: Polyphenol oxidase (PPO) is a metalloenzyme in tea plants that plays a crucial role in enhancing defensive responses, cultivating high-quality germplasm resources, and producing functional tea products. The oxidative polymerization of PPO in tea manufacturing can provide functional foods for preventing and treating chronic non-communicable diseases. However, more comprehensive research is needed to understand the relationship between PPO and tea. This article provides a comprehensive review of the classification, catalytic mechanism, and utilization of PPO in modulating tea flavors, compositions, and nutrition, as well as the techniques for modifying and applying PPO based on modern enzymology and synthetic biology.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Article
Infectious Diseases
Lirong Gao, Tingting Yang, Xiaohui Zhang, Wei Lei, Jian-an Huang
Summary: We retrospectively analyzed 3 cases of pulmonary nocardiosis using metagenomic next generation sequencing (mNGS) at the First Affiliated Hospital of Soochow University from January 2019 to April 2022. All patients had underlying diseases and were immunocompromised. Common symptoms included fever, cough, and expectoration. mNGS detected Nocardia spp. in bronchoalveolar lavage fluid (BALF) or blood samples. Patients were successfully treated with trimethoprim-sulfamethoxazole (TMP-SMX) and remained disease-free during follow-up.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Materials Science, Multidisciplinary
Jie Yuan, Tao Jin Wang, Jian Chen, Jian An Huang
Summary: The study of the creep properties of soils is important for settlement management and building safety. This paper analyzes the influence of mineral composition and content on the structure and creep properties of soft soil at the microscopic level. The energy scale method is proposed and used to analyze the results of direct shear creep test, revealing correlations between viscosity coefficient, adsorbed water, and soft soil creep.
FRONTIERS IN MATERIALS
(2023)
Article
Microbiology
Wei-Tao Gong, Xiao-Jie Zhao, Gao-Ming Wang, Xiao-Lin Ma, Jian-An Huang
Summary: Our study revealed the significant bactericidal effect of the zidovudine-amikacin combination against amikacin-resistant Enterobacteriales, and demonstrated the strong synergistic effect with amikacin. These findings suggest that the zidovudine-amikacin combination could be an effective alternative therapy for multidrug-resistant Enterobacteriales.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Changwei Liu, Haiyan Lin, Kuofei Wang, Zhixu Zhang, Jianan Huang, Zhonghua Liu
Summary: This study investigated the role of tea endophytes in black tea fermentation and their impact on black tea quality. The researchers collected fresh leaves and processed them into black tea, analyzing the biochemical composition and microbial community structure using high-throughput techniques. The results showed that certain bacteria and fungi dominated the fermentation process, and there were significant increases in enzymes and compounds related to fermentation. The study provides new insights into the changes in microbial communities during black tea fermentation and their impact on the quality of the final product.
Article
Chemistry, Applied
Xuexue Zheng, Tengfei Hu, He Xie, Xingchang Ou, Jianan Huang, Chao Wang, Zhonghua Liu, Qin Li
Summary: In this study, the key odor-active compounds of Fu brick tea were identified using headspace solid phase microextraction combined with gas chromatography-mass spectrometry and gas chromatography-olfactometry. A total of 43 volatile and 38 odor-active compounds were identified, with dihydroactindiolide, (E)-linalool oxide, and benzyl alcohol being the most abundant. Aroma recombination experiments verified that these compounds successfully simulated the overall aroma profile of Fu brick tea. This study provides a theoretical basis for understanding the formation of characteristic aromas in Fu brick tea.
Article
Agricultural Engineering
Chenyu Shao, Jianjiao Chen, Zhidong Lv, Xizhi Gao, Shuning Guo, Rong Xu, Zhiying Deng, Suhang Yao, Zhida Chen, Yankai Kang, Jianan Huang, Zhonghua Liu, Chengwen Shen
Summary: This year, extreme drought in China has caused a 30% reduction in the yield of tea plantations. Tea plants have the potential to resist drought through high levels of polyphenols and theanine. By regulating metabolite levels, tea plants can reduce the levels of toxic oxygen radicals under drought conditions.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Chemistry, Multidisciplinary
Xiaoyu Lu, Jianan Huang, Han Xie, Wei Huang, Zhigao Sun, Dongliang Zhang, Xiaowei Zhang, Mitang Wang
Summary: A liquid-phase reduction technique was used to obtain praseodymium-doped cuprous oxide photocatalyst. The 3% Pr : Cu2O photocatalyst showed the best photocatalytic performance due to the reduced recombination of photogenerated electron-hole pairs. The functioning mechanism of the Pr : Cu2O photocatalyst was examined.
Correction
Oncology
Yulong Fu, Anqi Wang, Jieqi Zhou, Wei Feng, Minhua Shi, Xiao Xu, Hongqing Zhao, Liming Cai, Jian Feng, Xuedong Lv, Xiaodong Zhang, Wenjing Xu, Zhengrong Zhang, Guoer Ma, Jian Wang, Tong Zhou, Dahai Zhao, Haohui Fang, Zeyi Liu, Jian-an Huang
FRONTIERS IN ONCOLOGY
(2023)
Article
Engineering, Environmental
Zhigao Sun, Xingchun Yang, Yuan Wu, Han Xie, Xiaoyu Lu, Jianan Huang, Dongliang Zhang, Mitang Wang
Summary: The focus of this work was on the process conditions and mechanism of phase separation activation treatment of an industrial byproduct called high-magnesium nickel slag (HMNS), as well as its potential as a precursor for a sustainable cement called magnesium phosphate cement (MPC). The study found that the phase separation structure in the HMNS played a crucial role in enhancing its pozzolanic activity, which is important for cement production. By optimizing the process conditions, the researchers were able to increase the dissolution of key elements in the HMNS and achieve a compressive strength comparable to ordinary Portland cement, while significantly reducing CO2 emissions.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
