Article
Oncology
Liangyi Zhu, Ying Yang, Haishuang Li, Luzheng Xu, Huanyu You, Yantao Liu, Zongran Liu, Xiaodan Liu, Danfeng Zheng, Juntao Bie, Jiaqi Li, Chao Song, Bao Yang, Jianyuan Luo, Qing Chang
Summary: This study explores the mechanism of tumor-associated microglia/macrophages (TAMs) activation/polarization in sonic hedgehog subgroup medulloblastoma (SHH-MB). M2-like macrophages were found to be enriched in SHH-MBs, and their polarization can be induced by SHH MB-derived exosomes. Downregulated let-7i-5p and miR-221-3p were identified as regulators of TAM polarization via upregulating peroxisome proliferator activated receptor gamma (PPAR gamma). The PPAR gamma antagonist improved the antitumor activity of SMO inhibitor by inhibiting M2-like TAMs. These findings suggest a potential immunotherapeutic strategy for SHH-MB.
Article
Cell Biology
Songjie Shen, Yu Song, Bin Zhao, Yali Xu, Xinyu Ren, Yidong Zhou, Qiang Sun
Summary: The study identified miR-7641 as a key miRNA in cancer-derived exosomes promoting tumor progression and metastasis via intercellular communication. Exosomal miR-7641 may serve as a non-invasive diagnostic biomarker and potential therapeutic target in breast cancer treatment.
CELL COMMUNICATION AND SIGNALING
(2021)
Review
Biochemistry & Molecular Biology
Kwang-Min Lee, Eun-Chan Seo, Jeong-Hyung Lee, Hyo-Jin Kim, Cheol Hwangbo
Summary: Syntenin serves as an important adaptor and scaffold protein, participating in multiple signaling pathways and modulating cellular physiology. It plays a significant role in regulating exosome trafficking and communication between cells. Targeting the regulation mechanism of exosomes by syntenin-1 and microRNAs may provide a novel treatment strategy for cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Siwen Qin, Jilong Cao, Xiaoxue Ma
Summary: Immunotherapy is increasingly used in clinical practice to treat tumors, but its efficacy varies and drug resistance often occurs. This review focuses on the significance of tumor-derived exosomes in clinical treatment and how modifying these exosomes may enhance immune effectiveness. It discusses exosome components, such as RNA, lipids, and proteins, as well as the role of membrane molecules. The importance of engineered exosomes for tumor immunotherapy is also highlighted, with the aim of proposing new strategies to improve efficacy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Guoqing Gu, Chenxi Hu, Kaiyuan Hui, Huiqin Zhang, Ting Chen, Xin Zhang, Xiaodong Jiang
Summary: This study revealed that high levels of plasma exosomal miR-136-5p are associated with poor clinical response to anlotinib. Anlotinib-resistant NSCLC cells transfer functional miR-136-5p to parental NSCLC cells via exosomes, promoting cell proliferation. Furthermore, miR-136-5p confers anlotinib resistance by targeting PPP2R2A and activating the Akt pathway.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Oncology
Rui Fu, Saiyue Liu, Mingjin Zhu, Jiajie Zhu, Mingxian Chen
Summary: Exosomes derived from IBS patients (IBS-exos) have regulatory effects on human colon epithelial cells. Apigenin counteracted the inhibitory effect of IBS-exo on autophagy of CACO-2 cells by regulating the miR-148b-3p/ATG14 signaling pathway.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2023)
Article
Chemistry, Analytical
Wenyu Sun, Yu Wang, Zhixue Zhu, Yeru Wang, Manru Zhang, Long Jiang, Su Liu, Jinghua Yu, Jiadong Huang
Summary: A novel double-positive EV membrane protein recognition mode was developed for accurate identification of EVs, showing excellent specificity and sensitivity. The approach integrates toehold activation and DNAzyme-catalyzed etching of nanorods, allowing for non-purified, low-cost, and visual EV identification.
ANALYTICAL CHEMISTRY
(2021)
Review
Oncology
Jiongjia Cheng, Xiaofeng Wang, Xuechun Yuan, Guangxiang Liu, Qian Chu
Summary: Cancer-derived exosomes have emerged as stable reservoirs for potential biomarkers in non-invasive cancer diagnosis. Exosomal proteins play pivotal roles in tumor development and progression through multiple signaling pathways. Furthermore, exosomal proteins have practical applications in detecting and monitoring several solid-tumor cancers.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Medical Laboratory Technology
Xinlei Zou, Ziyue Huang, Canghai Guan, Wujiang Shi, Jianjun Gao, Jiangang Wang, Yunfu Cui, Mei Wang, Yi Xu, Xiangyu Zhong
Summary: Pancreatic cancer (PC) is highly aggressive with a poor prognosis. The tumor microenvironment (TME) of PC is complex and diverse. Various cellular components in the microenvironment secrete different substances that promote tumor development. These substances can be released via exosomes, which are abundant extracellular vesicles (EVs) capable of carrying factors and enabling intercellular communication. MiRNAs are involved in regulating pathological and physiological processes and can be transported by exosomes to regulate the TME. Exosomal miRNAs hold promise as future targets for PC diagnosis and prognosis, offering potential new treatment strategies.
CLINICA CHIMICA ACTA
(2023)
Article
Biophysics
Jingjing Qian, Qinming Zhang, Mingdian Liu, Yixuan Wang, Meng Lu
Summary: This paper presents an on-chip exosomal miRNA amplification and detection system for rapid analysis, which quantitatively measures miRNAs extracted from exosomes using the EXPAR assay. The system is designed to allow storage of multiple oligonucleotide templates for the EXPAR, sample and reagent mixing, and simultaneous analysis of multiple exosomal miRNAs.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Oncology
Jingwen Jiang, Jiayu Li, Xiumei Zhou, Xueqin Zhao, Biao Huang, Yuan Qin
Summary: Exosomes play an important role in intercellular communication and can regulate the EMT process in tumor cells. They have potential applications in cancer diagnosis and treatment.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Israel Martinez-Espinosa, Jose Antonio Serrato, Blanca Ortiz-Quintero
Summary: The high mortality from lung cancer is mainly due to metastasis at the time of diagnosis. Recent research suggests that tumor cell exosomes play a significant role in tumor progression and metastasis. They release biologically active components to distant organs, directing tumor metastasis.
Review
Environmental Sciences
Esmail Pishbin, Fatemeh Sadri, Amin Dehghan, Mohammad Javad Kiani, Nader Hashemi, Iman Zare, Pegah Mousavi, Amid Rahi
Summary: Alzheimer's disease, a progressive neurological condition, requires early diagnosis for effective treatment management. This paper reviews recent advancements in detecting Alzheimer's disease related exosomal microRNAs using different methods such as electrochemical, fluorescent, and SPR. The use of microfluidic devices to isolate and detect these valuable biomarkers is also summarized. Challenges with the performance of novel technologies for isolating and detecting exosomal microRNAs are addressed.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Linyuan Shen, Jianfeng Ma, Yiting Yang, Tianci Liao, Jinyong Wang, Lei Chen, Shunhua Zhang, Ye Zhao, Lili Niu, Xiaoxia Hao, Anan Jiang, Xuewei Li, Mailin Gan, Li Zhu
Summary: In this study, exosomes from cooked meat were extracted using ultra-high-speed centrifugation, with approximately 80% of the vesicles falling within the range of 20-200 nm. Flow cytometry was used to evaluate the surface biomarkers of the isolated exosomes. Different microRNA profiles were observed in exosomes derived from cooked porcine muscle, fat, and liver. The chronic administration of cooked pork-derived exosomes to mice resulted in increased levels of miR-1, miR-133a-3p, miR-206, and miR-99a in the plasma. The study also revealed abnormal glucose metabolism, insulin resistance, and increased lipid droplets in the mice's liver, indicating that microRNAs from cooked pork may play a critical role in regulating metabolic disorders.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anelis Maria Marin, Sibelle Botogosque Mattar, Rafaela Ferreira Amatuzzi, Roger Chammas, Miyuki Uno, Dalila Luciola Zanette, Mateus Nobrega Aoki
Summary: Pancreatic cancer is a leading cause of cancer-related death worldwide, and early diagnosis is crucial. Plasma microRNAs have emerged as potential non-invasive biomarkers for diagnosis and prognosis. This study identified specific microRNAs that were overexpressed in the plasma exosomes of pancreatic cancer patients, suggesting their potential as diagnostic and prognostic markers.
Article
Biochemistry & Molecular Biology
Jose M. Murrieta-Coxca, Ruby N. Gutierrez-Samudio, Heba M. El-Shorafa, Tanja Groten, Sandra Rodriguez-Martinez, Mario E. Cancino-Diaz, Juan C. Cancino-Diaz, Rodolfo R. Favaro, Udo R. Markert, Diana M. Morales-Prieto
Summary: The study demonstrates that IL-36 cytokines are modulated by microbial components and play a role in trophoblast migration and interaction with endothelial cells. These cytokines may have a fundamental role in the placentation process and in response to infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Obstetrics & Gynecology
Rodolfo R. Favaro, Diana M. Morales-Prieto, Joerg Herrmann, Juergen Sonnemann, Ekkehard Schleussner, Udo R. Markert, Telma M. T. Zorn
Summary: This study found that exposure of human myometrium to high glucose conditions for 48 hours can regulate the expression of miRNAs, IGF1R, and its downstream targets, but does not significantly affect the expression of some related genes, indicating a certain response of the myometrium to short-term exposure to high glucose.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2021)
Review
Biochemistry & Molecular Biology
Diana M. Morales-Prieto, Paulina Fuentes-Zacarias, Jose M. Murrieta-Coxca, Ruby N. Gutierrez-Samudio, Rodolfo R. Favaro, Justine S. Fitzgerald, Udo R. Markert
Summary: Tobacco smoking is a major public health issue that has negative effects on pregnancy. This review examines the impact of smoking on immune regulation, placental function, and vascular development. The study aims to understand how maternal smoking affects placental function in both normal and pathological pregnancies. Smoking cessation is recommended for future mothers as the components of tobacco smoke can have detrimental effects on placental function.
MOLECULAR ASPECTS OF MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Maryam Noor Rizwan, Yunxia Ma, Miljana Nenkov, Lai Jin, Desiree Charlotte Schroeder, Martin Westermann, Nikolaus Gassler, Yuan Chen
Summary: Exosomes are crucial intercellular communicators that play a significant role in tumor growth, progression, and drug resistance. Lung cancer, a major clinical challenge, may benefit from lung tumor-derived exosomes in activating the tumor microenvironment and promoting metastasis.
MOLECULAR CARCINOGENESIS
(2022)
Article
Developmental Biology
Jose Martin Murrieta-Coxca, Leonie Aengenheister, Astrid Schmidt, Udo R. Markert, Tina Buerki-Thurnherr, Diana M. Morales-Prieto
Summary: The physical connection between mother and offspring during pregnancy allows for bi-directional exchange of cells, which can persist long-term in the recipient individual and play a role in immune adaptation and tolerogenic responses. However, the mechanisms of cell transfer across the placenta barrier are still poorly understood.
Article
Cell Biology
Diana M. Morales-Prieto, Jose M. Murrieta-Coxca, Milan Stojiljkovic, Celia Diezel, Priska E. Streicher, Julian A. Henao-Restrepo, Franziska Roestel, Julia Lindner, Otto W. Witte, Sebastian Weis, Christian Schmeer, Manja Marz
Summary: Extracellular vesicles, specifically small EVs, are involved in neuroinflammation and neurodegenerative diseases. Aging brains show increased neuroinflammation and glial activation, which can be influenced by EVs. Our study demonstrates that sEVs from aged mice can cross the blood-brain barrier and induce glial cell activation in young animals.
Article
Biochemistry & Molecular Biology
Alena Gschwind, Christian Marx, Marie D. Just, Paula Severin, Hannah Behring, Lisa Marx-Blumel, Sabine Becker, Linda Rothenburger, Martin Foester, James F. Beck, Juergen Sonnemann
Summary: This study identified the relationship between autophagy and the cell cycle, and provided clear evidence of high autophagy in G2/M-phase cells using the Cyto-ID technique.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Review
Immunology
Jose M. Murrieta-Coxca, Paulina Fuentes-Zacarias, Stephanie Ospina-Prieto, Udo R. Markert, Diana M. Morales-Prieto
Summary: The concept of biological identity has been a central issue in immunology. The expanded immunotolerance observed in pregnancy challenges the assumption that foreign entities should be rejected by the immune system. Microchimeric cells play a crucial role in enhancing immune tolerance and genetic fitness during pregnancy, but they may also cause abnormal immune activation. Studies have revealed a synergistic effect between microchimerism and cellular communication through extracellular vesicles (EVs), which participate in feto-maternal tolerance and antigen presentation. This review discusses the current understanding of microchimerism and the potential role of EVs in mediating allorecognition and tropism of microchimeric cells during human pregnancy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Christian Marx, Juergen Sonnemann, Oliver D. K. Maddocks, Lisa Marx-Bluemel, Mandy Beyer, Doerte Hoelzer, Rene Thierbach, Claudia Maletzki, Michael Linnebacher, Thorsten Heinzel, Oliver H. Kraemer
Summary: Chemotherapeutics that cause DNA replication stress increase the metabolism of colorectal cancer (CRC) cells, and glucose restriction might improve the effectiveness of classical chemotherapy against p53-positive cells.
CANCER & METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Cesar G. Pelcastre-Rodriguez, Ernesto A. Vazquez-Sanchez, Jose M. Murrieta-Coxca, Sandra Rodriguez-Martinez, Juan C. Cancino-Diaz, Mario E. Cancino-Diaz
Summary: The study found that IL-36 is involved in the activation of mesangial cells and the production of inflammation, with specific members potentially playing a key role in the inflammation associated with infections in the glomerular environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yunxia Ma, Rumyana Marinkova, Miljana Nenkov, Lai Jin, Otmar Huber, Juergen Sonnemann, Natalia Peca, Nikolaus Gassler, Yuan Chen
Summary: PD-L1 may play an oncogenic role in non-small cell lung cancer (NSCLC) by activating the Wnt/β-catenin signaling pathway and may act as a potential diagnostic marker for squamous cell carcinoma (SCC) of the lung.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Max-Johann Sturm, Julian Andres Henao-Restrepo, Sabine Becker, Hans Proquitte, James F. Beck, Juergen Sonnemann
Summary: The present study aimed to explore the effectiveness of combined inhibition of ATR and ribonucleotide reductase (RNR) as a new strategy for the treatment of Ewing's sarcoma. The results showed that the combination of ATR and RNR inhibitors had synergistic effects on Ewing's sarcoma cells in vitro, inducing apoptosis. This study reveals the potential of combining ATR and RNR inhibitors as a new treatment strategy for Ewing's sarcoma.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Obstetrics & Gynecology
Elizabeth Soczewski, Jose Martin Murrieta-Coxca, Lucas Miranda, Paulina Fuentes-Zacarias, Ruby Gutierrez-Samudio, Esteban Grasso, Marcelo Marti, Claudia Perez Leiros, Diana Morales-Prieto, Udo R. Markert, Rosanna Ramhorst
Summary: The study reveals the role of miRNAs in regulating endoplasmic reticulum stress and unfolded protein response in decidualized cells and endometrium associated with reproductive failures. The findings highlight the potential involvement of miR-17-5p, miR-21-5p, and miR-193b-3p in the regulation of ERS/UPR pathways and inflammation during embryo implantation. The expression of these miRNAs differs in stromal cells from patients with recurrent pregnancy loss and recurrent implantation failure, suggesting alterations in the regulation of ERS/UPR pathways.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
