Review
Biochemistry & Molecular Biology
Ellen Youngsoo Rim, Hans Clevers, Roel Nusse
Summary: The Wnt pathway plays a central role in developmental and disease-related processes, regulating the generation and spatial arrangement of different cell types. Through regulation of cell fate, mitotic activity, and cell polarity, Wnt signaling coordinates development and tissue homeostasis, and its dysregulation is implicated in various disorders. This review summarizes the advances in our understanding of this pathway, from Wnt protein production to signal transduction in receiving cells. The evolutionary history of this pathway, as well as its regulators, and the remaining gaps in knowledge are discussed, highlighting directions for future research.
ANNUAL REVIEW OF BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
S. Zachary Swartz, Tzer Han Tan, Margherita Perillo, Nikta Fakhri, Gary M. Wessel, Athula H. Wikramanayake, Iain M. Cheeseman
Summary: In meiotic oocytes of the sea star Patifia miniata, the distribution dynamics of the Disheveled protein are regulated by meiotic cell cycle progression and influenced by association with Lamp1+ endosomes and pre-localized Frizzled receptors within the oocyte cortex.
Article
Cell & Tissue Engineering
Yiwei Li, Maorong Chen, Jiliang Hu, Ren Sheng, Qirong Lin, Xi He, Ming Guo
Summary: Intracellular crowding, influenced by external physical cues, plays a significant role in regulating Wnt/beta-catenin signaling and promoting the growth of intestinal organoids. This indicates a potential link between physical cellular properties and cellular functionalities, with implications for stem cell and organoid expansion.
Article
Multidisciplinary Sciences
Gonzalo J. Beitia, Trevor J. Rutherford, Stefan M. Freund, Hugh R. Pelham, Mariann Bienz, Melissa Gammons
Summary: The study reveals that intermolecular contacts in the DEP dimer of Dishevelled protein play a crucial role in its stability and function in relaying canonical Wnt signals, and these contacts are mediated by a phosphorylation site that can regulate the protein's activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Editorial Material
Biochemistry & Molecular Biology
Charles A. Ettensohn
Summary: The establishment of early polarity in animal embryos is influenced by the dynamic redistribution of the Wnt signaling effector Dishevelled, a process regulated by the cell cycle and the Wnt receptor Frizzled 1.
Article
Chemistry, Multidisciplinary
Xinna Wang, Wanjing Ou, Di Feng, Bo Gao, Barbara Pui Chan
Summary: The Wnt signaling pathway plays a crucial role in various cellular processes. This study successfully immobilized a concentration gradient of Wnt3a on a protein substrate using the MMM technology. The Wnt3a gradient showed bioactivity and induced cell proliferation and Wnt/beta-catenin signaling activation in mouse L cells. Furthermore, human hair dermal papilla cells cultured in the Wnt3a gradient demonstrated Wnt3a-gradient-dependent hair-inductive phenotype. This research contributes to the development of a biochip for drug screening and therapeutics in hair regeneration.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Yuri Lee, Hai-long Piao, Jongchan Kim
Summary: The Wnt signaling pathway is crucial for regulating various cellular processes, and dysregulation of this pathway has been linked to human diseases, including cancer. In this study, researchers aimed to identify the deubiquitinases (DUBs) that regulate the pathway through the essential component LEF1. They discovered that OTUD7B interacts with LEF1 and activates Wnt signaling. Furthermore, OTUD7B promotes the nuclear localization of LEF1, leading to increased interaction with beta-catenin. This study suggests that OTUD7B may serve as a potential therapeutic target in diseases where Wnt signaling is dysregulated, such as cancer.
Article
Biochemistry & Molecular Biology
Min Gyu Lee, Hwamok Oh, Jong Woo Park, Jueng Soo You, Jeung-Whan Han
Summary: Nuclear S6K1 functions in the regulation of Wnt target gene expression and has an impact on cell proliferation and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Yang Zhou, Jiang Xu, Haichang Luo, Xiangjing Meng, Ming Chen, Di Zhu
Summary: Abnormal activation of the Wnt/beta-catenin signaling pathway is closely related to tumorigenesis and immune surveillance, leading to increased resistance to immunotherapy.
Article
Biochemistry & Molecular Biology
Tao Li, Xiaofei Weng, Siya Cheng, Dongxing Wang, Guanchang Cheng, Hai Gao, Yanming Li
Summary: The study found that in TGFβ1-induced cardiac hypertrophy, the Wnt/beta-catenin signaling pathway is activated, which is associated with the expression of c-Myc and the upregulation of Wnt3a.
Review
Biochemistry & Molecular Biology
Michael Latour, Nam-Gu Her, Santosh Kesari, Elmar Nurmemmedov
Summary: The WNT signaling pathway plays a crucial role in glioblastoma genesis and therapy resistance, presenting a therapeutic challenge. Current targeted therapies under clinical investigation may offer new treatment options.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Jung Ki Min, Hwee-Seon Park, Yoon-Beom Lee, Jae-Gyu Kim, Jong-Il Kim, Jae-Bong Park
Summary: Src, a non-receptor tyrosine kinase, plays a critical role in cancer progression for various tissues. It regulates Wnt signaling through multiple signaling molecules, and aberrant regulation of this interaction can lead to the development of various diseases, particularly cancer.
Article
Biochemistry & Molecular Biology
Jacob P. Mahoney, Elise S. Bruguera, Mansi Vasishtha, Lauren B. Killingsworth, Saw Kyaw, William Weis
Summary: In the Wnt-beta-catenin pathway, Fzd-Dvl binding is enhanced by increased concentration of PI(4,5)P-2, generated by Dvl-associated lipid kinases in response to Wnt. However, binding to Fzd does not promote Dvl DEP domain dimerization.
Article
Biochemistry & Molecular Biology
Mingliang He, Xiaoyang Lv, Xiukai Cao, Zehu Yuan, Tesfaye Getachew, Yutao Li, Shanhe Wang, Wei Sun
Summary: SRY-box transcription factor 18 (SOX18) is expressed in dermal papilla cells (DPCs) of sheep hair follicles and plays a crucial role in promoting DPC proliferation through activating the Wnt/β-Catenin signaling pathway. Inhibition of the Wnt/β-Catenin signaling pathway can be rescued by SOX18, suggesting the essential role of SOX18 in governing DPC proliferation and hair growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Nives Pecina-Slaus, Sara Anicic, Anja Bukovac, Anja Kafka
Summary: In the search for improved antitumor therapies, inhibition of the Wnt signaling pathway has emerged as a promising target due to its altered functioning in human tumors. The pathway's frequent mutations and epigenetic changes in tumors have promoted the investigation of Wnt signaling inhibitors. Studies have shown that knocking down specific components of the pathway inhibits tumor growth. This review discusses the inhibitors of the canonical Wnt pathway, evaluates their effectiveness as therapeutics, and examines their potential side effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Hana Plesingerova, Pavlina Janovska, Archana Mishra, Lucie Smyckova, Lucie Poppova, Antonin Libra, Karla Plevova, Petra Ovesna, Lenka Radova, Michael Doubek, Sarka Pavlova, Sarka Pospisilova, Vitezslav Bryja
Article
Hematology
Hana Plesingerova, Pavlina Janovska, Archana Mishra, Lucie Smyckova, Lucie Poppova, Antonin Libra, Karla Plevova, Petra Ovesna, Lenka Radova, Michael Doubek, Sarka Pavlova, Sarka Pospisilova, Vitezslav Bryja
Article
Hematology
Pavlina Janovska, Jan Verner, Jiri Kohoutek, Lenka Bryjova, Michaela Gregorova, Marta Dzimkova, Hana Skabrahova, Tomasz Radaszkiewicz, Petra Ovesna, Olga Vondalova Blanarova, Tereza Nemcova, Zuzana Hoferova, Katerina Vasickova, Lucie Smyckova, Alexander Egle, Sarka Pavlova, Lucie Poppova, Karla Plevova, Sarka Pospisilova, Vitezslav Bryja
Article
Biochemistry & Molecular Biology
Jakub Harnos, Jan Rynes, Pavlina Viskova, Silvie Foldynova-Trantirkova, Lola Bajard-Esner, Lukas Trantirek, Vitezslav Bryja
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Jakub Harnos, Maria Consuelo Alonso Canizal, Miroslav Jurasek, Jitender Kumar, Cornelia Holler, Alexandra Schambony, Katerina Hanakova, Ondrej Bernatik, Zbynek Zdrahal, Kristina Gomoryova, Tomas Gybel, Tomasz Witold Radaszkiewicz, Marek Kravec, Lukas Trantirek, Jan Rynes, Zankruti Dave, Ana Iris Fernandez-Llamazares, Robert Vacha, Konstantinos Tripsianes, Carsten Hoffmann, Vitezslav Bryja
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Karol Kaiser, Daniel Gyllborg, Jan Prochazka, Alena Salasova, Petra Kompanikova, Francisco Lamus Molina, Rocio Laguna-Goya, Tomasz Radaszkiewicz, Jakub Harnos, Michaela Prochazkova, David Potesil, Roger A. Barker, Angel Gato Casado, Zbynek Zdrahal, Radislav Sedlacek, Ernest Arenas, J. Carlos Villaescusa, Vitezslav Bryja
NATURE COMMUNICATIONS
(2019)
Article
Cell & Tissue Engineering
Veronika Fedorova, Tereza Vanova, Lina Elrefae, Jakub Pospisil, Martina Petrasova, Veronika Kolajova, Zuzana Hudacova, Jana Baniariova, Martin Barak, Lucie Peskova, Tomas Barta, Marketa Kaucka, Michael Killinger, Josef Vecera, Ondrej Bernatik, Lukas Cajanek, Hana Hribkova, Dasa Bohaciakova
STEM CELL RESEARCH
(2019)
Article
Cell Biology
Katerina Hanakova, Ondrej Bernatik, Marek Kravec, Miroslav Micka, Jitender Kumar, Jakub Harnos, Petra Ovesna, Petra Paclikova, Matej Radsetoulal, David Potesil, Konstantinos Tripsianes, Lukas Cajanek, Zbynek Zdrahal, Vitezslav Bryja
CELL COMMUNICATION AND SIGNALING
(2019)
Article
Cell Biology
Petra Pejskova, Madeline Louise Reilly, Lucia Bino, Ondrej Bernatik, Linda Dolanska, Ranjani Sri Ganji, Zbynek Zdrahal, Alexandre Benmerah, Lukas Cajanek
JOURNAL OF CELL BIOLOGY
(2020)
Article
Cell Biology
Ondrej Bernatik, Petra Pejskova, David Vyslouzil, Katerina Hanakova, Zbynek Zdrahal, Lukas Cajanek
MOLECULAR BIOLOGY OF THE CELL
(2020)
Article
Cell Biology
Ondrej Bernatik, Petra Paclikova, Ranjani Sri Ganji, Vitezslav Bryja
Review
Biochemistry & Molecular Biology
Karol Kaiser, Vitezslav Bryja
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Pavlina Janovska, Emmanuel Normant, Hari Miskin, Vitezslav Bryja
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)